FastQCFastQC Report
Thu 26 May 2016
SRR937268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671890
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103201.5359657086725507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79131.177722543868788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65580.9760526276622661No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32300.4807334533926684No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18700.2783193677536501No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17980.26760332792570213No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.2606081352602361No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15000.22325082974891725No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14510.2159579693104526No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12110.18023783655062584No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11270.16773579008468648No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10390.15463840807275No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA10040.14942922204527528No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9660.1437735343583027No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT9610.1430293649258063No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9480.1410945244013157No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8940.13305749453035468No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8830.1314203217788626No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT8020.11936477697242108No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7980.11876944142642397No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7700.11460209260444418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG753.0751893E-431.6700154
TACCTGG4950.028.7909242
GTAGGAC1551.4078814E-624.5187233
CCTATAC1751.4827128E-724.4311543
AGTACTT71050.022.96518112-13
GAGTACT70050.022.95396612-13
GTATCAA123700.022.8218211
GTACATG74500.022.5191481
TACATGG75250.022.095362
CTATACT2858.367351E-1121.6689594
GGTATCA94200.021.6441171
TATACGC1553.9001294E-521.4538823
GTACTTT75600.021.26727314-15
CGGTTAC905.171756E-621.110228-29
TAAGGTG2509.460564E-920.902215
GCGTGCG1150.003721016720.652829
ACATGGG75400.020.6023123
TACTTTT79900.020.27134114-15
TAGGACA4000.020.1896344
CCTACAC1908.235338E-620.0021153
Produced by FastQC (version 0.11.5)