##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937268_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 671890 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22094688118591 33.0 31.0 34.0 31.0 34.0 2 32.388664811204215 34.0 31.0 34.0 31.0 34.0 3 32.416993853160484 34.0 31.0 34.0 31.0 34.0 4 36.084369465239845 37.0 35.0 37.0 35.0 37.0 5 36.031456041911625 37.0 35.0 37.0 35.0 37.0 6 36.10859366860647 37.0 35.0 37.0 35.0 37.0 7 36.051587313399516 37.0 35.0 37.0 35.0 37.0 8 36.03995892184733 37.0 35.0 37.0 35.0 37.0 9 37.725462501302296 39.0 37.0 39.0 35.0 39.0 10-11 37.64967107711084 39.0 37.0 39.0 35.0 39.0 12-13 37.55332792570212 39.0 37.0 39.0 35.0 39.0 14-15 38.62974370804744 40.0 38.0 41.0 34.0 41.0 16-17 38.655830567503614 40.0 38.0 41.0 34.5 41.0 18-19 38.76728407923916 40.0 38.0 41.0 35.0 41.0 20-21 38.740775275714775 40.0 38.0 41.0 34.5 41.0 22-23 38.595387637857385 40.0 38.0 41.0 34.0 41.0 24-25 38.50434967033294 40.0 38.0 41.0 34.0 41.0 26-27 38.3989373260504 40.0 38.0 41.0 34.0 41.0 28-29 38.265161708017686 40.0 38.0 41.0 34.0 41.0 30-31 38.1669849231273 40.0 37.0 41.0 34.0 41.0 32-33 37.953014630371044 40.0 36.5 41.0 33.5 41.0 34-35 37.74949247644704 40.0 36.0 41.0 33.0 41.0 36-37 37.52279911890339 40.0 35.5 41.0 33.0 41.0 38-39 37.355523969697416 40.0 35.0 41.0 33.0 41.0 40-41 37.10902677521618 40.0 35.0 41.0 33.0 41.0 42-43 36.78115837413863 40.0 35.0 41.0 31.5 41.0 44-45 36.29928336483651 39.0 35.0 41.0 30.5 41.0 46-47 35.65817172453824 39.0 35.0 41.0 28.0 41.0 48-49 35.11767476819122 39.0 35.0 41.0 24.5 41.0 50-51 34.81734138028546 39.0 35.0 41.0 23.5 41.0 52-53 34.574765958713485 39.0 35.0 40.0 23.0 41.0 54-55 34.351175043533914 38.0 34.5 40.0 22.5 41.0 56-57 34.085644227477715 38.0 34.0 40.0 20.5 41.0 58-59 33.83828974981024 38.0 34.0 40.0 20.0 41.0 60-61 33.50565047850094 37.5 33.5 40.0 18.5 41.0 62-63 33.16145946509101 37.0 33.0 40.0 16.0 41.0 64-65 32.761886618345265 36.0 33.0 39.5 11.5 41.0 66-67 32.35271993927577 36.0 32.5 39.0 7.5 40.5 68-69 31.926689636696484 35.0 32.0 39.0 4.5 40.0 70-71 31.52044605515784 35.0 31.5 38.0 2.0 40.0 72-73 31.06432674991442 35.0 31.0 37.0 2.0 39.5 74-75 30.610537439164148 35.0 31.0 37.0 2.0 39.0 76-77 29.336927175579333 33.5 29.5 35.5 2.0 38.0 78-79 29.893059131703104 34.0 30.0 36.0 2.0 38.0 80-81 29.69197190016223 34.0 30.0 35.5 2.0 37.0 82-83 29.34176353272113 34.0 30.0 35.0 2.0 37.0 84-85 28.98063224634985 34.0 29.5 35.0 2.0 36.5 86-87 28.583794966437956 34.0 29.0 35.0 2.0 36.0 88-89 28.112831713524535 34.0 29.0 35.0 2.0 36.0 90-91 27.619225617288546 34.0 27.0 35.0 2.0 35.0 92-93 27.323328967539332 33.5 27.0 35.0 2.0 35.0 94-95 26.925787703344298 33.0 26.0 35.0 2.0 35.0 96-97 26.494051853726056 33.0 25.0 35.0 2.0 35.0 98-99 25.848507940287845 33.0 24.0 35.0 2.0 35.0 100-101 23.594981321347245 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09869500882846438 1101 2 -0.06386794456314249 1101 3 -0.02453415336652398 1101 4 -0.032209656946399434 1101 5 0.04729481555716575 1101 6 -0.013814600355082973 1101 7 -0.2123223453550196 1101 8 -0.19473909440132076 1101 9 0.10362663110381476 1101 10-11 0.05530313739096471 1101 12-13 -0.10574098672220344 1101 14-15 -0.13612546850829688 1101 16-17 -0.020565380276501344 1101 18-19 -0.01968055706197447 1101 20-21 -0.0223534313791518 1101 22-23 -0.07598323721945377 1101 24-25 -0.08665997429253025 1101 26-27 0.07420846717018748 1101 28-29 -0.15967455135980657 1101 30-31 0.0997704003481914 1101 32-33 0.011770204374066395 1101 34-35 0.06818860947598893 1101 36-37 0.07541036167552306 1101 38-39 -0.11788988554266666 1101 40-41 -0.03494712235405473 1101 42-43 0.08389649591374138 1101 44-45 0.26403363390152634 1101 46-47 -0.050656030974899124 1101 48-49 -0.14405894651465445 1101 50-51 0.050315634779487084 1101 52-53 -0.0014785484939068283 1101 54-55 -0.17543050890287049 1101 56-57 0.10119736502264942 1101 58-59 -0.07546579503912909 1101 60-61 -0.17403882883232313 1101 62-63 -0.06020075570693706 1101 64-65 -0.018038465670812798 1101 66-67 -0.11878606668657454 1101 68-69 -0.3561421949186361 1101 70-71 -0.3675122351239857 1101 72-73 -0.1256439294000593 1101 74-75 -0.34009900778805147 1101 76-77 -0.22073647074906333 1101 78-79 -0.2174297323861616 1101 80-81 -0.04695270776245053 1101 82-83 0.0017062096785167569 1101 84-85 -0.1281596295691365 1101 86-87 -0.3256440989489775 1101 88-89 -0.26486508225589844 1101 90-91 -0.29179789079053364 1101 92-93 -0.3241728964016133 1101 94-95 -0.3992378624203816 1101 96-97 -0.5895201170278739 1101 98-99 -0.4836135723192214 1101 100-101 -0.0797643666090373 1102 1 -0.05447404565565961 1102 2 -0.0779432177318995 1102 3 -0.21762601900796597 1102 4 -0.06951034492697516 1102 5 -0.06950809824292037 1102 6 -0.06428809286823167 1102 7 -0.1826339600859015 1102 8 -0.041930514878856684 1102 9 -0.0804931584550701 1102 10-11 -0.0931003429813515 1102 12-13 -0.17832293734947768 1102 14-15 -0.1843812354042882 1102 16-17 -0.0792177477390581 1102 18-19 -0.19182543767711024 1102 20-21 -0.14118184092994568 1102 22-23 -0.24835879367432057 1102 24-25 -0.22339813697969646 1102 26-27 -0.2663034713162489 1102 28-29 -0.4928170038082058 1102 30-31 -0.3536027279399505 1102 32-33 -0.23788378186632286 1102 34-35 -0.16957950060089644 1102 36-37 -0.3913358949007488 1102 38-39 -0.3067891166232002 1102 40-41 -0.21875125023750286 1102 42-43 -0.0018906348025709008 1102 44-45 -0.3207963944270844 1102 46-47 -0.30507731790326886 1102 48-49 -0.15274975995051676 1102 50-51 0.004170349469895029 1102 52-53 0.05678733576348094 1102 54-55 0.1864877428170857 1102 56-57 0.16058020597773037 1102 58-59 0.1385730556286191 1102 60-61 -0.12137189236934631 1102 62-63 0.028573020092331092 1102 64-65 0.03826869742105288 1102 66-67 0.1409306471944447 1102 68-69 0.02042801956942597 1102 70-71 0.09707497653121777 1102 72-73 0.16221523692935946 1102 74-75 0.16859382912008058 1102 76-77 0.2844254070332113 1102 78-79 0.20926998432772237 1102 80-81 0.035602937725208506 1102 82-83 0.12382073820947426 1102 84-85 0.18930698313826966 1102 86-87 0.04981320578594506 1102 88-89 0.041203311107114615 1102 90-91 -0.12226935983141018 1102 92-93 -0.2743125159888251 1102 94-95 0.09664639496529048 1102 96-97 0.4272382803820811 1102 98-99 0.3782702803719964 1102 100-101 0.9152963375593153 1103 1 0.019876419742963947 1103 2 0.1947034840082864 1103 3 0.001180132347762708 1103 4 -0.03649537123211388 1103 5 0.004437672700021267 1103 6 0.033328256787775956 1103 7 -0.17946520249788023 1103 8 -0.0290248086870335 1103 9 0.032198059675238255 1103 10-11 0.023160280248106346 1103 12-13 0.0414018704206498 1103 14-15 0.09744596006314055 1103 16-17 0.04443461972349638 1103 18-19 -0.07468055706197418 1103 20-21 -0.11663914566486966 1103 22-23 -0.10384038007659768 1103 24-25 -0.3595171171496645 1103 26-27 -0.15293438997266406 1103 28-29 -0.15110312278837768 1103 30-31 -0.15451531393753015 1103 32-33 -0.031086938483078086 1103 34-35 -0.12395424766686602 1103 36-37 0.0696960759612466 1103 38-39 0.0835386858859053 1103 40-41 0.17005287764594357 1103 42-43 0.23603935305659718 1103 44-45 0.18403363390152805 1103 46-47 0.3093439690251003 1103 48-49 0.1380839106282039 1103 50-51 0.43174420620805876 1103 52-53 0.49495002293466683 1103 54-55 0.36385520538284766 1103 56-57 0.33691165073693696 1103 58-59 0.21453420496087716 1103 60-61 0.34167545688196554 1103 62-63 0.21051353000735418 1103 64-65 0.3055329629006138 1103 66-67 0.4590710761705665 1103 68-69 0.4081435193670764 1103 70-71 0.5182020505903004 1103 72-73 0.42149892774279607 1103 74-75 0.32061527792623323 1103 76-77 0.6306921006795072 1103 78-79 0.43899883904240866 1103 80-81 0.3116187208089798 1103 82-83 0.2759919239642308 1103 84-85 0.3546975132880057 1103 86-87 0.3093559010510205 1103 88-89 0.08584920345839109 1103 90-91 0.2724878234951795 1103 92-93 0.2951128178840996 1103 94-95 0.48504785186533184 1103 96-97 0.31047988297212825 1103 98-99 0.40924357053792093 1103 100-101 1.1609499191052493 1104 1 -0.030359363890880786 1104 2 -0.015019123481316399 1104 3 -0.19808477749967324 1104 4 -5.453018839887136E-4 1104 5 0.017308685182683803 1104 6 -0.016893657220549585 1104 7 -0.22291873925238548 1104 8 -0.06007890022655715 1104 9 -0.12863411785596668 1104 10-11 -0.20191600269225063 1104 12-13 -0.18979091737407572 1104 14-15 -0.1510214463168893 1104 16-17 -0.29057924990201656 1104 18-19 -0.027822027242272895 1104 20-21 -0.05130627465238291 1104 22-23 -0.2073147212693769 1104 24-25 -0.12948937789862924 1104 26-27 -0.3871715605690582 1104 28-29 -0.2629269785442716 1104 30-31 -0.14993140270312466 1104 32-33 -0.25542119645810857 1104 34-35 -0.2369778260302482 1104 36-37 -0.6060459490040842 1104 38-39 -0.5988468897035588 1104 40-41 -0.6243091195801256 1104 42-43 -0.5155903279420144 1104 44-45 -0.32454472948266044 1104 46-47 -0.3934784997682428 1104 48-49 -0.41413522945502024 1104 50-51 -0.2614388728488066 1104 52-53 -0.2300153030015366 1104 54-55 -0.3036551968362957 1104 56-57 -0.06560568629496544 1104 58-59 -0.1647307048865585 1104 60-61 0.0036865525823799317 1104 62-63 -0.07149063088030516 1104 64-65 -0.05668617718260549 1104 66-67 -0.12362656599308508 1104 68-69 0.00932937234904685 1104 70-71 -0.03360793554007557 1104 72-73 0.04640877517691422 1104 74-75 0.15605910594287664 1104 76-77 0.17351456947285016 1104 78-79 -0.10179172961223415 1104 80-81 -0.18496241650031564 1104 82-83 -0.19767659198583942 1104 84-85 -0.16219569059548888 1104 86-87 -0.021892365245790302 1104 88-89 0.05731938376352019 1104 90-91 -0.20767167719830226 1104 92-93 0.01799769999228573 1104 94-95 -0.06313522719153042 1104 96-97 -0.09311235003758256 1104 98-99 -0.06687986912920962 1104 100-101 0.39949360842563664 1105 1 0.12250386282174475 1105 2 0.2000168025656066 1105 3 0.055177714455361127 1105 4 0.06863459048458509 1105 5 0.10837480749751904 1105 6 -0.1294019467181684 1105 7 -0.042449284706222556 1105 8 -0.020797929783142877 1105 9 0.018319357277498227 1105 10-11 0.10268073158801627 1105 12-13 0.09085381480912957 1105 14-15 0.12748122099402792 1105 16-17 -0.06550594042156632 1105 18-19 0.11882236455801376 1105 20-21 0.0586207777685388 1105 22-23 0.030087687624394732 1105 24-25 -0.08497550925122255 1105 26-27 0.036335205030361806 1105 28-29 -0.047612290630404175 1105 30-31 -0.04250342596653667 1105 32-33 0.1850655902293994 1105 34-35 0.18977333645563732 1105 36-37 0.05949458492759163 1105 38-39 0.02309137579120346 1105 40-41 -0.18854172239435485 1105 42-43 0.08446268571828597 1105 44-45 -0.029457198256437778 1105 46-47 0.10247211732249895 1105 48-49 0.5083337595099238 1105 50-51 0.4930266966372372 1105 52-53 0.5344221164265051 1105 54-55 0.5065461181372299 1105 56-57 0.6109102402997024 1105 58-59 0.6113002738708104 1105 60-61 0.6027937321187196 1105 62-63 0.7754994256349974 1105 64-65 0.45957688794594986 1105 66-67 0.5685058938211824 1105 68-69 0.2317109181178374 1105 70-71 0.40152564519033973 1105 72-73 0.48873245585079417 1105 74-75 0.5152687133483553 1105 76-77 0.5253374764602867 1105 78-79 0.32964361035008594 1105 80-81 0.39229472322687187 1105 82-83 0.4647799553968319 1105 84-85 0.3960958610615286 1105 86-87 0.37443951982998414 1105 88-89 0.7219140835712246 1105 90-91 0.8038025524897385 1105 92-93 0.9026284334392081 1105 94-95 1.005757100303775 1105 96-97 0.7190089984264887 1105 98-99 0.8160831836751363 1105 100-101 1.1917720025225371 1106 1 -0.13246069920503345 1106 2 0.005185651770787558 1106 3 0.1517818458365099 1106 4 0.08083477421518381 1106 5 -0.0033487389357631514 1106 6 -0.044215078537988006 1106 7 -0.13879375400216531 1106 8 -0.18009275050810913 1106 9 0.04486193694162921 1106 10-11 -0.28515213258312855 1106 12-13 -0.1075142483280942 1106 14-15 -0.09873953505541522 1106 16-17 -0.05762854026853148 1106 18-19 -0.08989972422674697 1106 20-21 0.02793048972066714 1106 22-23 -0.20089058928162018 1106 24-25 -0.0241614686141034 1106 26-27 -0.16947453741239116 1106 28-29 -0.14221190939925776 1106 30-31 0.020996740256535418 1106 32-33 -0.0012114652812371673 1106 34-35 0.02804615081888784 1106 36-37 0.08031771373769914 1106 38-39 0.1375335055710991 1106 40-41 0.039316675215182784 1106 42-43 0.03528721119566569 1106 44-45 0.25970308996847535 1106 46-47 0.40504729638114867 1106 48-49 0.47871949540604675 1106 50-51 0.3621855291807563 1106 52-53 0.5354361755547075 1106 54-55 0.5570759664906362 1106 56-57 0.2650078850465647 1106 58-59 0.44624370884611153 1106 60-61 0.5014861761487523 1106 62-63 0.5563642970924221 1106 64-65 0.46727036078863904 1106 66-67 0.625663026758339 1106 68-69 0.7918344936647479 1106 70-71 0.9617475775877082 1106 72-73 0.6938320618332554 1106 74-75 0.7228557640788544 1106 76-77 0.643966657364107 1106 78-79 0.47207514906432735 1106 80-81 0.46277134085281446 1106 82-83 0.14387696082416213 1106 84-85 0.29541877250298754 1106 86-87 0.20497853504185315 1106 88-89 0.5371881155922793 1106 90-91 0.5978305212437363 1106 92-93 0.7421739854473586 1106 94-95 0.7182895334752146 1106 96-97 0.552538061892232 1106 98-99 0.6179275713348922 1106 100-101 1.1169391599898866 1107 1 -0.08408382192532571 1107 2 -0.047025223845935216 1107 3 -0.07743417285590226 1107 4 -0.06609583897719773 1107 5 -0.14990261184490095 1107 6 -0.20547704432311775 1107 7 -0.1587343508120469 1107 8 -0.11853757422981204 1107 9 -0.07568734278041944 1107 10-11 -0.0845341263003192 1107 12-13 -0.13655369379684856 1107 14-15 0.04548825201402451 1107 16-17 0.04560495688974697 1107 18-19 -0.12123386051158747 1107 20-21 -0.19242261867207588 1107 22-23 -0.14499317680238022 1107 24-25 -0.14798914989182776 1107 26-27 -0.38548847299741595 1107 28-29 -0.43503900273380935 1107 30-31 -0.3547969354537983 1107 32-33 -0.05529177184889278 1107 34-35 -0.1620345439054134 1107 36-37 -0.03219634267060201 1107 38-39 -0.38868895005250437 1107 40-41 -0.2381687165852 1107 42-43 -0.0809894912232707 1107 44-45 -0.47900183676696173 1107 46-47 -0.4448140898328248 1107 48-49 0.15403596675761122 1107 50-51 -0.14672685206518565 1107 52-53 -0.40393908250287325 1107 54-55 -0.26672850149690674 1107 56-57 -0.41642395635719964 1107 58-59 -0.2345887730161209 1107 60-61 -0.2756681408572703 1107 62-63 -0.19702398704585988 1107 64-65 -0.519892880015 1107 66-67 -0.4445295864682919 1107 68-69 -0.35515700684613805 1107 70-71 -0.36718189016199076 1107 72-73 -0.3405045803452964 1107 74-75 -0.49493627147934305 1107 76-77 -0.3182213664808877 1107 78-79 -0.4597557896849338 1107 80-81 -0.2433189131804987 1107 82-83 -0.2750049577352449 1107 84-85 -0.1483194424711023 1107 86-87 -0.18219155558511346 1107 88-89 0.08024210932468989 1107 90-91 0.02611382232581505 1107 92-93 0.25237261129669264 1107 94-95 0.06258829232528029 1107 96-97 0.3089421710251372 1107 98-99 0.3682895654706826 1107 100-101 1.213647249060422 1108 1 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0.13224966963792184 2316 72-73 0.20177715139180918 2316 74-75 0.2314472736458697 2316 76-77 0.1029366164633494 2316 78-79 -0.09804770991425116 2316 80-81 -0.27017100631782753 2316 82-83 0.13549700689627997 2316 84-85 0.2205241585528519 2316 86-87 -0.00263981858514839 2316 88-89 0.07034358548085962 2316 90-91 0.12609670525013073 2316 92-93 0.4706344872259933 2316 94-95 0.21198204127143327 2316 96-97 0.226953933266401 2316 98-99 0.35554373105156145 2316 100-101 -0.3791196368070011 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 10.0 5 21.0 6 26.0 7 89.0 8 232.0 9 411.0 10 720.0 11 1310.0 12 2227.0 13 3289.0 14 4085.0 15 6038.0 16 8860.0 17 13140.0 18 10896.0 19 5126.0 20 3926.0 21 4065.0 22 4064.0 23 4429.0 24 4798.0 25 5680.0 26 6560.0 27 7921.0 28 9728.0 29 12096.0 30 14846.0 31 18172.0 32 23131.0 33 30423.0 34 42152.0 35 63762.0 36 90903.0 37 136496.0 38 119836.0 39 12419.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.54013055891907 19.738733224012954 15.933371163547164 18.787765053520815 2 15.388233192933368 22.632871452172232 39.73596868535028 22.242926669544122 3 19.587432466624 28.68401077557338 30.647725074044857 21.080831683757758 4 11.911175936537232 17.72343687210704 37.7527571477474 32.61263004360833 5 11.822568450130678 40.31532604230543 32.5525841077818 15.309521399782103 6 28.318177082558154 37.90635371861465 17.651103603268393 16.124365595558796 7 26.58188096265758 36.15919272499963 20.147940883180283 17.11098542916251 8 27.896233014332704 32.62185774457129 19.242584351605174 20.239324889490838 9 28.438286028963073 16.5149057137329 20.54383902126836 34.50296923603566 10-11 24.852356784592715 28.27784309931685 25.613642114036523 21.25615800205391 12-13 27.595560907287204 26.29325934845479 27.088080803871108 19.0230989403869 14-15 23.788566580839127 26.047492893181918 28.719879742219707 21.444060783759248 16-17 19.761196029111908 29.907871824256947 28.922442661745222 21.40848948488592 18-19 19.705829823334177 26.757802616499728 34.30658292279986 19.229784637366233 20-21 21.65332122817723 24.862700739704415 34.36953965678906 19.114438375329296 22-23 20.696637591728937 25.53519243262793 34.218976624702606 19.549193350940527 24-25 20.451550152518546 25.70929124084386 34.65389564144486 19.185262965192727 26-27 20.251366299145097 25.578667873216965 35.01610387445527 19.153861953182673 28-29 19.603658150876026 26.07976697855759 35.05851954881119 19.25805532175519 30-31 19.746059055929667 25.816337085692048 34.904600986622036 19.533002871756253 32-33 19.5773861792853 26.183080563782763 34.57306999657682 19.66646326035512 34-35 20.17160547113367 25.708226048906813 34.63141288008454 19.48875559987498 36-37 20.42002442365895 25.5114866358233 34.51162728395071 19.55686165656704 38-39 20.096653373231003 25.963352386678977 34.46308932102271 19.476904919067312 40-41 19.827843459145377 26.26187231958874 34.32221268856365 19.588071532702227 42-43 19.74845173299474 26.48565531829065 33.48714906754173 20.278743881172883 44-45 20.188617605450904 26.321629258426064 33.01537383062815 20.474379305494885 46-47 20.68887764366191 26.949649496197292 32.215020315825505 20.14645254431529 48-49 20.614683951242018 27.185327955468903 31.544598074089507 20.655390019199572 50-51 20.484603134441652 27.57259372814002 31.331691199452294 20.611111937966037 52-53 20.68590096593192 28.129306880590573 30.43132060307491 20.753471550402598 54-55 20.178526246855885 27.811695366801114 30.650776168718092 21.35900221762491 56-57 20.910565717602584 27.915432585691107 30.029394692583605 21.144607004122697 58-59 21.261218354194884 27.275298039857717 30.450296923603563 21.013186682343836 60-61 20.906249534894105 27.63324353688848 31.153685871199155 20.30682105701826 62-63 21.0411674530057 27.612778877494826 31.026135230469272 20.3199184390302 64-65 20.599130810102846 28.018648885978358 31.011475092649093 20.370745211269703 66-67 20.8453764753159 27.968640700114598 30.313592998853977 20.87238982571552 68-69 20.87856643200524 27.945794698536965 30.437050707705133 20.73858816175267 70-71 20.966378425039814 28.236244028040304 30.13008081680037 20.667296730119514 72-73 20.784801083510693 27.898019020970693 30.33182514995014 20.985354745568472 74-75 20.374912560091683 28.96121388917829 29.76938189286937 20.894491657860662 76-77 20.36337793388799 28.233713851969817 30.371861465418448 21.03104674872375 78-79 20.283677387667623 28.206849335456695 30.355936239563025 21.153537037312656 80-81 20.390912202890355 28.77107859917546 29.922755212906875 20.91525398502731 82-83 20.276608807278006 28.38734163085338 30.051682759390523 21.284366802478093 84-85 20.05651252108801 28.158029667213135 29.862484214223684 21.922973597475167 86-87 20.233594784860617 28.08904731429252 29.658872732143653 22.01848516870321 88-89 20.211046451055974 27.87859619878254 29.68640700114602 22.223950349015464 90-91 20.079469449511446 27.596841762914902 29.96291475305155 22.3607740345221 92-93 20.091356483028427 27.843559836784753 30.095248584741025 21.969835095445795 94-95 19.754424087276192 27.451442944529607 30.259119796395247 22.535013171798955 96-97 19.566893390287103 27.3196505380345 30.40386075101579 22.709595320662608 98-99 19.75055440622721 27.456056795011087 29.8149994790814 22.978389319680304 100-101 19.440597121118866 27.115455175354835 30.483899417034383 22.96004828649191 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1239.0 1 867.0 2 901.0 3 1235.5 4 900.5 5 729.5 6 1387.5 7 3187.0 8 4790.0 9 4973.5 10 3912.0 11 2809.5 12 2559.5 13 2525.5 14 2518.0 15 2555.5 16 2563.5 17 2518.5 18 2540.0 19 2590.0 20 2711.0 21 2903.0 22 3094.0 23 3421.0 24 3842.0 25 4302.5 26 4931.5 27 5787.5 28 6475.5 29 7481.5 30 8784.5 31 9573.5 32 10891.0 33 12554.0 34 14049.0 35 15276.5 36 17271.5 37 19357.0 38 20092.0 39 20092.5 40 19852.5 41 20360.5 42 20748.5 43 21359.0 44 22370.0 45 23039.5 46 22669.0 47 24318.5 48 31393.0 49 30717.5 50 23786.0 51 20052.5 52 17207.5 53 16310.0 54 15506.0 55 14245.0 56 13007.0 57 11859.0 58 11182.0 59 10508.5 60 9346.0 61 8333.5 62 7053.0 63 4963.0 64 3219.5 65 2434.5 66 1799.5 67 1318.0 68 912.0 69 526.5 70 365.5 71 307.5 72 271.5 73 262.0 74 225.5 75 166.0 76 122.0 77 73.5 78 36.0 79 24.0 80 25.5 81 17.5 82 9.0 83 7.0 84 4.0 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06980309276816146 2 0.0 3 0.0 4 0.0 5 0.0020836744109898942 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0061021893464704045 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.185863757460298E-4 24-25 6.697524892467517E-4 26-27 2.976677729985563E-4 28-29 0.0030510946732352023 30-31 2.2325082974891723E-4 32-33 0.0 34-35 0.0 36-37 7.441694324963908E-5 38-39 6.697524892467517E-4 40-41 2.2325082974891723E-4 42-43 0.013469466728184673 44-45 2.2325082974891723E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.7208471624819537E-4 84-85 5.209186027474736E-4 86-87 0.0 88-89 0.0 90-91 0.00885561624670705 92-93 0.0024557591272380895 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.009897453452201999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 671890.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.576756634774675 #Duplication Level Percentage of deduplicated Percentage of total 1 76.8969609872688 38.89198881806857 2 12.744614558942807 12.891625379033128 3 4.291925396830266 6.512149988702793 4 1.866921239882542 3.7769128482332444 5 1.0421020220786184 2.6353070179638434 6 0.639299345492739 1.9400212448254197 7 0.44240752845566617 1.5662876530066066 8 0.3187902247121081 1.2898700490247539 9 0.23395677760686767 1.0649497503670784 >10 1.343829284558357 12.515961377129923 >50 0.11483681239873211 3.9871151905921733 >100 0.056385146598802026 5.257424147043094 >500 0.004132942682632506 1.5828113535950008 >1k 0.0029521019161660752 2.3860869957364126 >5k 5.90420383233215E-4 2.1606323080721874 >10k+ 2.952101916166075E-4 1.5408558786058304 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10320 1.5359657086725507 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7913 1.177722543868788 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6558 0.9760526276622661 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3230 0.4807334533926684 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1870 0.2783193677536501 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1798 0.26760332792570213 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1751 0.2606081352602361 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1500 0.22325082974891725 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1451 0.2159579693104526 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1211 0.18023783655062584 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1127 0.16773579008468648 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1039 0.15463840807275 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1004 0.14942922204527528 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 966 0.1437735343583027 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 961 0.1430293649258063 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 948 0.1410945244013157 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 894 0.13305749453035468 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 883 0.1314203217788626 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 802 0.11936477697242108 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 798 0.11876944142642397 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 770 0.11460209260444418 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.4883388649927816E-4 0.0 0.0 0.0 0.0 8 1.4883388649927816E-4 0.0 0.0 0.0 0.0 9 1.4883388649927816E-4 0.0 0.0 0.0 0.0 10-11 1.4883388649927816E-4 0.0 0.0 0.0 0.0 12-13 1.4883388649927816E-4 0.0 0.0 0.0 0.0 14-15 1.4883388649927816E-4 0.0 0.0 0.0 0.0 16-17 1.4883388649927816E-4 0.0 0.0 0.0 0.0 18-19 1.4883388649927816E-4 0.0 0.0 0.0 0.0 20-21 1.4883388649927816E-4 0.0 0.0 0.0 0.0 22-23 1.4883388649927816E-4 0.0 0.0 1.4883388649927816E-4 0.0 24-25 1.4883388649927816E-4 0.0 0.0 1.4883388649927816E-4 0.0 26-27 1.4883388649927816E-4 0.0 0.0 5.209186027474736E-4 0.0 28-29 1.4883388649927816E-4 0.0 0.0 0.0015627558082424208 0.0 30-31 2.976677729985563E-4 0.0 0.0 0.0035720132759826757 0.0 32-33 2.976677729985563E-4 0.0 0.0 0.006251023232969683 0.0 34-35 2.976677729985563E-4 0.0 0.0 0.009302117906204884 0.0 36-37 4.4650165949783446E-4 0.0 0.0 0.012204378692940807 0.0 38-39 4.4650165949783446E-4 0.0 0.0 0.015478724195924928 0.0 40-41 5.209186027474736E-4 0.0 0.0 0.019646073017904716 0.0 42-43 5.953355459971127E-4 0.0 0.0 0.026938933456369347 0.0 44-45 5.953355459971127E-4 0.0 0.0 0.043459494857789216 0.0 46-47 5.953355459971127E-4 0.0 0.0 0.07962612927711382 0.0 48-49 5.953355459971127E-4 0.0 0.0 0.10864873714447305 0.0 50-51 5.953355459971127E-4 0.0 0.0 0.15061989313726948 0.0 52-53 5.953355459971127E-4 0.0 0.0 0.19668398100879608 0.0 54-55 5.953355459971127E-4 0.0 0.0 0.24066439446933277 0.0 56-57 5.953355459971127E-4 0.0 0.0 0.29640268496331246 0.0 58-59 5.953355459971127E-4 0.0 0.0 0.3804938308354046 0.0 60-61 6.697524892467516E-4 0.0 0.0 0.4916727440503654 0.0 62-63 7.441694324963907E-4 0.0 0.0 0.6303115093244429 0.0 64-65 7.441694324963907E-4 0.0 0.0 0.7469972763398771 0.0 66-67 8.930033189956689E-4 0.0 0.0 0.9147330664245635 0.0 68-69 8.930033189956689E-4 0.0 0.0 1.1139472235038475 0.0 70-71 8.930033189956689E-4 0.0 0.0 1.2830969355102768 0.0 72-73 8.930033189956689E-4 0.0 0.0 1.5211567369658723 0.0 74-75 8.930033189956689E-4 0.0 0.0 1.7401658009495602 0.0 76-77 8.930033189956689E-4 0.0 0.0 1.9446635609995684 0.0 78-79 8.930033189956689E-4 0.0 0.0 2.1935138192263617 0.0 80-81 8.930033189956689E-4 0.0 0.0 2.459182306627573 0.0 82-83 8.930033189956689E-4 0.0 0.0 2.780216999806516 0.0 84-85 8.930033189956689E-4 0.0 0.0 3.18965902156603 0.0 86-87 9.67420262245308E-4 0.0 0.0 3.668606468320707 0.0 88-89 0.0010418372054949471 0.0 0.0 4.224575451338761 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACACG 75 3.0751893E-4 31.670015 4 TACCTGG 495 0.0 28.790924 2 GTAGGAC 155 1.4078814E-6 24.518723 3 CCTATAC 175 1.4827128E-7 24.431154 3 AGTACTT 7105 0.0 22.965181 12-13 GAGTACT 7005 0.0 22.953966 12-13 GTATCAA 12370 0.0 22.821821 1 GTACATG 7450 0.0 22.519148 1 TACATGG 7525 0.0 22.09536 2 CTATACT 285 8.367351E-11 21.668959 4 GGTATCA 9420 0.0 21.644117 1 TATACGC 155 3.9001294E-5 21.453882 3 GTACTTT 7560 0.0 21.267273 14-15 CGGTTAC 90 5.171756E-6 21.1102 28-29 TAAGGTG 250 9.460564E-9 20.90221 5 GCGTGCG 115 0.0037210167 20.65282 9 ACATGGG 7540 0.0 20.602312 3 TACTTTT 7990 0.0 20.271341 14-15 TAGGACA 400 0.0 20.189634 4 CCTACAC 190 8.235338E-6 20.002115 3 >>END_MODULE