FastQCFastQC Report
Thu 26 May 2016
SRR937267_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937267_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498989
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37400.2495014973425422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28630.19099539756462522No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24100.16077502903623708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20030.1336233955019016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21200.052.23741
GTATCAA40700.043.091681
ATCAACG51900.032.865733
TATCAAC52500.032.852132
TCAACGC51800.032.747924
CAACGCA53400.031.8556885
AACGCAG55450.030.5881946
ACGCAGA65050.025.7097037
CGCAGAG65650.025.4747318
TACATGG50050.025.251882
GTACATG51850.024.841741
ACATGGG53400.023.4007993
GCAGAGT74100.021.8644169
GTGTAGC9750.021.4491231
CATGGGA27000.020.590524
AGAGTAC65200.020.58361610-11
GTATAGG4300.019.8959881
GAGTACT41400.019.7352512-13
TATACCG1507.7044277E-419.0066365
GTACCGT1250.006024474319.0040936
Produced by FastQC (version 0.11.5)