##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937267_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1498989 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.384299684654124 31.0 31.0 34.0 30.0 34.0 2 31.499774848247718 33.0 31.0 34.0 30.0 34.0 3 31.53811335506798 33.0 31.0 34.0 28.0 34.0 4 35.177385557866 37.0 35.0 37.0 33.0 37.0 5 35.179465626498924 37.0 35.0 37.0 33.0 37.0 6 35.19787470088173 37.0 35.0 37.0 32.0 37.0 7 35.12343786378686 37.0 35.0 37.0 32.0 37.0 8 35.093166127303135 37.0 35.0 37.0 32.0 37.0 9 36.64119416486712 39.0 37.0 39.0 32.0 39.0 10-11 36.50611245312675 39.0 37.0 39.0 32.0 39.0 12-13 36.338539842520525 39.0 37.0 39.0 32.0 39.0 14-15 37.377451402245114 40.0 37.0 41.0 32.0 41.0 16-17 37.27479954822884 40.0 37.0 41.0 32.0 41.0 18-19 37.16353088648415 39.0 37.0 41.0 31.0 41.0 20-21 36.98602925038142 39.0 36.0 41.0 31.0 41.0 22-23 37.072466175535645 39.0 36.5 41.0 31.0 41.0 24-25 37.02940815442942 39.0 36.0 41.0 31.0 41.0 26-27 36.93760161015191 39.0 36.0 41.0 31.0 41.0 28-29 36.77522650266279 39.0 36.0 41.0 30.0 41.0 30-31 36.50379622532253 39.0 36.0 40.5 30.0 41.0 32-33 36.2478874094473 39.0 35.0 40.0 30.0 41.0 34-35 36.22629752453153 39.0 35.0 40.0 30.0 41.0 36-37 36.0525070564227 39.0 35.0 40.0 29.0 41.0 38-39 35.87507179839211 39.0 35.0 40.0 28.5 41.0 40-41 35.61190675848855 38.5 35.0 40.0 27.0 41.0 42-43 35.32222784823638 38.0 35.0 40.0 26.0 41.0 44-45 35.077726721143385 38.0 34.0 40.0 25.5 41.0 46-47 34.696291300336426 38.0 34.0 40.0 24.0 41.0 48-49 34.344978849077606 38.0 33.0 40.0 23.5 41.0 50-51 33.383130563333026 37.5 32.0 39.5 22.5 40.5 52-53 33.489501590738826 37.5 33.0 39.5 22.0 40.0 54-55 33.73963518077851 38.0 33.0 40.0 21.5 41.0 56-57 33.40856003613102 37.5 33.0 40.0 19.5 41.0 58-59 33.06996015314322 37.0 32.0 40.0 18.0 41.0 60-61 32.67810404212439 37.0 31.5 39.5 15.5 41.0 62-63 32.222634722469614 36.0 31.0 39.0 11.0 41.0 64-65 31.719862520672265 36.0 31.0 39.0 8.0 40.0 66-67 31.22612173938568 35.0 30.0 38.5 7.0 40.0 68-69 30.668176684418633 35.0 29.0 38.0 2.0 40.0 70-71 30.135919943375168 34.5 29.0 37.5 2.0 39.5 72-73 29.572468176884556 34.0 28.0 37.0 2.0 39.0 74-75 28.952954624750415 34.0 27.5 36.0 2.0 39.0 76-77 28.349556601149175 34.0 26.0 36.0 2.0 38.5 78-79 27.72496028990206 33.0 26.0 35.0 2.0 37.5 80-81 27.05141598770905 33.0 25.0 35.0 2.0 37.0 82-83 26.404063672248427 32.0 23.5 35.0 2.0 36.0 84-85 25.717408199793326 32.0 21.0 35.0 2.0 36.0 86-87 25.01089867904301 31.0 19.0 34.0 2.0 35.5 88-89 24.31713441526255 31.0 14.0 34.0 2.0 35.0 90-91 23.620829772600068 31.0 6.0 34.0 2.0 35.0 92-93 22.8266985281413 30.0 2.0 34.0 2.0 35.0 94-95 21.924548479008184 29.5 2.0 34.0 2.0 35.0 96-97 20.92178027990866 29.0 2.0 34.0 2.0 35.0 98-99 19.617867776214503 28.0 2.0 34.0 2.0 35.0 100-101 16.69927030818772 20.0 2.0 31.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39794039725046204 1101 2 0.6108971707613726 1101 3 0.533154821898826 1101 4 0.34952070028945315 1101 5 0.5071111153073744 1101 6 0.5292162164490577 1101 7 0.5044889876861944 1101 8 0.46510207706501205 1101 9 0.49711271255738154 1101 10-11 0.7642331570796941 1101 12-13 0.5579138963129395 1101 14-15 0.716890873475073 1101 16-17 0.8437703726128944 1101 18-19 0.9531120456694495 1101 20-21 0.9473275152124003 1101 22-23 0.9107383055829246 1101 24-25 1.010163909572718 1101 26-27 0.9014165996255201 1101 28-29 0.9030623016429757 1101 30-31 0.9778046530359319 1101 32-33 0.807292684488921 1101 34-35 0.9228859928919135 1101 36-37 1.1281601310641207 1101 38-39 0.9544671358049044 1101 40-41 1.1061076190349581 1101 42-43 0.9977966807763039 1101 44-45 1.0773343163768558 1101 46-47 0.9553350048065496 1101 48-49 1.0947057379740457 1101 50-51 1.1961094185696908 1101 52-53 1.2775659413649194 1101 54-55 1.3411259749307192 1101 56-57 1.263013962835025 1101 58-59 1.4408119453837713 1101 60-61 1.5495751997540381 1101 62-63 1.7457639530844702 1101 64-65 1.5640239833560905 1101 66-67 1.8090539639051677 1101 68-69 1.9315418715910404 1101 70-71 1.7608957323601366 1101 72-73 1.69918376465942 1101 74-75 1.7414877040660066 1101 76-77 1.7330809108059526 1101 78-79 2.133365431513951 1101 80-81 2.287669178282588 1101 82-83 2.137017796025475 1101 84-85 2.236293997171284 1101 86-87 2.4760178645091884 1101 88-89 2.5940301349601 1101 90-91 2.5289241939655938 1101 92-93 2.755779111344566 1101 94-95 2.711525335510551 1101 96-97 2.6470004865175696 1101 98-99 2.911057406305382 1101 100-101 2.677818558507713 1102 1 0.4166979711938268 1102 2 0.40976833063015405 1102 3 0.19645265360915687 1102 4 0.27164043414082073 1102 5 0.15716964240635178 1102 6 0.24153642883946702 1102 7 0.16026293889503052 1102 8 0.1818433047096235 1102 9 0.27617859115545684 1102 10-11 0.25152389947581355 1102 12-13 0.208457568676792 1102 14-15 0.5575810390644378 1102 16-17 0.5835389686437864 1102 18-19 0.619714026300791 1102 20-21 0.49912038502212397 1102 22-23 0.36018874954245206 1102 24-25 0.49658139049205374 1102 26-27 0.714857600800201 1102 28-29 0.6694865333070652 1102 30-31 0.7440905666883282 1102 32-33 0.6957866903643364 1102 34-35 0.9099986766535864 1102 36-37 1.052252444918011 1102 38-39 0.9533982678862145 1102 40-41 1.0938360243934397 1102 42-43 1.0368101017523301 1102 44-45 1.1826681272858082 1102 46-47 1.0133707651736898 1102 48-49 0.9203001441459264 1102 50-51 0.8169472334754175 1102 52-53 0.9166529599098538 1102 54-55 1.1469842588377261 1102 56-57 1.1166786675879976 1102 58-59 1.218112204884882 1102 60-61 1.1162496425079311 1102 62-63 1.2103426715164787 1102 64-65 1.1356665200671365 1102 66-67 1.080622799824905 1102 68-69 1.2218791198058092 1102 70-71 1.2418573934975257 1102 72-73 1.4652322667992195 1102 74-75 1.3677498559226677 1102 76-77 1.4404361259111056 1102 78-79 1.5424254677243674 1102 80-81 1.5403271549997442 1102 82-83 1.5813525380004485 1102 84-85 1.620097577336896 1102 86-87 1.664112812598642 1102 88-89 1.799407608942893 1102 90-91 1.8351819485481542 1102 92-93 1.997955369780417 1102 94-95 1.678433267325964 1102 96-97 1.6898078466873478 1102 98-99 2.013044230998659 1102 100-101 1.8834798940816597 1103 1 0.2769088306802736 1103 2 0.3931575230176527 1103 3 0.3368669157022701 1103 4 0.2847163159979118 1103 5 0.1166968194910325 1103 6 0.2262153777436282 1103 7 0.18106114924388095 1103 8 0.27727008949758414 1103 9 0.4682955750096909 1103 10-11 0.3843227217751206 1103 12-13 0.3822611244737075 1103 14-15 0.4559476176534716 1103 16-17 0.3423159998329979 1103 18-19 0.290007478836138 1103 20-21 0.27197075705558404 1103 22-23 0.18830817714587766 1103 24-25 0.30831341292753933 1103 26-27 0.3463580546972267 1103 28-29 0.21697196402292462 1103 30-31 0.274276745523764 1103 32-33 0.1895861327194197 1103 34-35 0.38880330640329674 1103 36-37 0.40838076404004653 1103 38-39 0.48372983561414173 1103 40-41 0.444306334664482 1103 42-43 0.1376801048323415 1103 44-45 0.411731620185563 1103 46-47 0.302334988361352 1103 48-49 0.390109406076931 1103 50-51 0.37124365255206015 1103 52-53 0.35604605500127917 1103 54-55 0.38370929044840807 1103 56-57 0.33466828874608723 1103 58-59 0.444972068229454 1103 60-61 0.26805590952906755 1103 62-63 0.2603883651684029 1103 64-65 0.244617511345826 1103 66-67 0.640134475515481 1103 68-69 0.6933449032644994 1103 70-71 0.7019473826364155 1103 72-73 0.9246809237503264 1103 74-75 0.7247798901270528 1103 76-77 0.579192853313522 1103 78-79 0.44483760622780366 1103 80-81 0.6330842552461462 1103 82-83 0.7643576402893864 1103 84-85 0.894618148598731 1103 86-87 1.2298686119437363 1103 88-89 1.2481643566085694 1103 90-91 1.068402449293842 1103 92-93 1.1567666458794967 1103 94-95 1.1184048784983176 1103 96-97 0.8279100707827958 1103 98-99 1.0537997263948462 1103 100-101 1.2037690913323402 1104 1 0.2709243251092417 1104 2 0.3606512714349712 1104 3 0.25679522033635394 1104 4 0.31643716304779446 1104 5 0.33387403207650124 1104 6 0.27247313287151087 1104 7 0.3874741651972897 1104 8 0.24431673105240748 1104 9 0.395842501446765 1104 10-11 0.5317403828918685 1104 12-13 0.5189640476821111 1104 14-15 0.7757338768330087 1104 16-17 0.5984152496430752 1104 18-19 0.5481365146679948 1104 20-21 0.6429101958375512 1104 22-23 0.6622701374522819 1104 24-25 0.6379736117120345 1104 26-27 0.5120012555075562 1104 28-29 0.6019636153903605 1104 30-31 0.5351853616291038 1104 32-33 0.6233826634867015 1104 34-35 0.7398890243065566 1104 36-37 0.8647611372357886 1104 38-39 0.9717400518081121 1104 40-41 0.9720526786756309 1104 42-43 0.9043645106945917 1104 44-45 0.9191731797576068 1104 46-47 0.8205081018077891 1104 48-49 0.8920630256641715 1104 50-51 0.6070213019569692 1104 52-53 0.7820924037604655 1104 54-55 1.0011510082883746 1104 56-57 1.0161214651831614 1104 58-59 1.1014960695588982 1104 60-61 1.0859884557432977 1104 62-63 0.953122087313254 1104 64-65 1.093965249697309 1104 66-67 1.2542466320741035 1104 68-69 1.2731801859310004 1104 70-71 1.1068095708486219 1104 72-73 1.3238355752443773 1104 74-75 1.4173949113349522 1104 76-77 1.5224419353596055 1104 78-79 1.57832076652155 1104 80-81 1.6459989171858744 1104 82-83 1.8410809800589512 1104 84-85 1.7763010113487923 1104 86-87 1.9160698258586528 1104 88-89 2.121353178145668 1104 90-91 2.248567720565049 1104 92-93 2.6182679753299887 1104 94-95 2.434041550757115 1104 96-97 2.186932924669854 1104 98-99 2.2689689147969716 1104 100-101 1.9854859816843238 1105 1 0.41244010183802615 1105 2 0.28224618169310034 1105 3 0.42820012337137925 1105 4 0.4020639417134291 1105 5 0.46805543569710295 1105 6 0.4140817915430972 1105 7 0.3156783093349773 1105 8 0.27629923718639304 1105 9 0.4347831380982612 1105 10-11 0.3490250995217892 1105 12-13 0.3976536296285005 1105 14-15 0.4925856315945012 1105 16-17 0.4973681825436529 1105 18-19 0.4737524586785682 1105 20-21 0.5716772894153976 1105 22-23 0.5728847047355927 1105 24-25 0.6336725176853335 1105 26-27 0.6623240791940503 1105 28-29 0.5841741301920678 1105 30-31 0.731075500678557 1105 32-33 0.8541280481499598 1105 34-35 0.7071967021071117 1105 36-37 0.8006332279564532 1105 38-39 0.6992246673523113 1105 40-41 0.6145045828026028 1105 42-43 0.6551662657389699 1105 44-45 0.8082723757866006 1105 46-47 0.8723892676129026 1105 48-49 0.8286569552398504 1105 50-51 0.5849682055464669 1105 52-53 0.8173425155016858 1105 54-55 1.0742764432304668 1105 56-57 1.0713958865333737 1105 58-59 1.1691843747321897 1105 60-61 1.2550717816900843 1105 62-63 0.9630875654053668 1105 64-65 0.997743328804436 1105 66-67 1.1474173104234602 1105 68-69 1.2054930594625688 1105 70-71 1.2774591598106646 1105 72-73 1.288723371971539 1105 74-75 1.292583032630283 1105 76-77 1.3010443874428965 1105 78-79 1.523436732220297 1105 80-81 1.410689351571623 1105 82-83 1.5709529444224586 1105 84-85 1.6653325497189364 1105 86-87 1.773152804794723 1105 88-89 1.835202300151991 1105 90-91 2.049590082010649 1105 92-93 2.186397116120151 1105 94-95 1.8866257904359074 1105 96-97 1.8351017830989136 1105 98-99 1.9594198024088776 1105 100-101 1.9707106805488088 1106 1 0.08767150550544756 1106 2 -0.535786969989342 1106 3 -0.17770913324877924 1106 4 0.03032383348043055 1106 5 0.08145862019033245 1106 6 0.04712883928208811 1106 7 0.047669540852275816 1106 8 0.013557239847237668 1106 9 0.04354688619849867 1106 10-11 -0.3754620247283711 1106 12-13 -0.15156208356825118 1106 14-15 -0.08621802919967791 1106 16-17 -0.048975521146026324 1106 18-19 0.17351927953543367 1106 20-21 0.025560136426214797 1106 22-23 -0.05568767075622105 1106 24-25 0.0655468045359271 1106 26-27 0.07328113162030547 1106 28-29 0.10317716507186958 1106 30-31 0.09095069657271893 1106 32-33 0.05995326558655023 1106 34-35 -0.03191347681349299 1106 36-37 0.005632730823258214 1106 38-39 0.15908494925049865 1106 40-41 0.24429540809105532 1106 42-43 0.04854658210507523 1106 44-45 0.11036033522053401 1106 46-47 0.2068585897599462 1106 48-49 0.5052372469860202 1106 50-51 0.23519688681779627 1106 52-53 0.29120230500128486 1106 54-55 0.34333013835050963 1106 56-57 0.06854612965517504 1106 58-59 -0.10732797547684214 1106 60-61 0.009817273165431573 1106 62-63 0.3282915557278443 1106 64-65 0.3599420305765939 1106 66-67 0.3348405506903056 1106 68-69 0.2724696847330321 1106 70-71 0.32945503123781705 1106 72-73 0.2953376108132666 1106 74-75 0.343688325441736 1106 76-77 0.3679821889778552 1106 78-79 0.3714110328012268 1106 80-81 0.39497236713425465 1106 82-83 0.3710720196600157 1106 84-85 0.24616972202530363 1106 86-87 0.2744107885171623 1106 88-89 0.7784036485666093 1106 90-91 0.8871032797134184 1106 92-93 0.9421250374878873 1106 94-95 0.9767363907360789 1106 96-97 0.7922020288247538 1106 98-99 1.0243198312899509 1106 100-101 0.5254474130106601 1107 1 0.14879839459505462 1107 2 0.30071354680411844 1107 3 0.20536611374124902 1107 4 0.20429513514813635 1107 5 0.09984901807474245 1107 6 0.13709745921076433 1107 7 0.0839633402619171 1107 8 0.0018711895460086225 1107 9 0.21449098870052552 1107 10-11 0.12148330690403952 1107 12-13 0.0631558216955952 1107 14-15 0.1052967808903631 1107 16-17 0.33740115144902205 1107 18-19 0.2846405816687181 1107 20-21 0.38619677009879894 1107 22-23 0.2441451861338919 1107 24-25 0.2057323846016672 1107 26-27 0.18881054228953076 1107 28-29 0.24870853117580793 1107 30-31 0.26109167410383805 1107 32-33 0.2460487280468513 1107 34-35 0.37667553736806525 1107 36-37 0.16475436817391653 1107 38-39 0.44248889550398474 1107 40-41 0.5490312847310648 1107 42-43 0.6703538693584363 1107 44-45 0.4831718778722873 1107 46-47 0.5746404749852445 1107 48-49 0.6568895893174584 1107 50-51 0.4454354498526314 1107 52-53 0.6873528920972731 1107 54-55 0.7594880816056531 1107 56-57 0.6911281415181492 1107 58-59 0.9419103361449501 1107 60-61 0.7029476447911449 1107 62-63 0.7457880406011625 1107 64-65 0.6453535564674873 1107 66-67 0.8639388159403687 1107 68-69 1.0495957544023788 1107 70-71 1.0753760875450205 1107 72-73 1.2830719137939077 1107 74-75 1.084774802213964 1107 76-77 1.138721436414837 1107 78-79 1.1396990031531153 1107 80-81 1.2469627203230118 1107 82-83 1.4344504521463044 1107 84-85 1.3854655792971933 1107 86-87 1.306746694954267 1107 88-89 1.459965761553491 1107 90-91 1.536740593132361 1107 92-93 1.6520418849546665 1107 94-95 1.6933026473822288 1107 96-97 1.529554090877216 1107 98-99 1.563462978422443 1107 100-101 1.5589924299112035 1108 1 0.30397374895912677 1108 2 0.33364673173255355 1108 3 0.3088851930386767 1108 4 0.1903475322633028 1108 5 0.19245802772076104 1108 6 0.3278731116874667 1108 7 0.2698400321355052 1108 8 0.18351203488541756 1108 9 0.19685561882678115 1108 10-11 0.3275336787009593 1108 12-13 0.3468158560371748 1108 14-15 0.4368131757528104 1108 16-17 0.45269972155657 1108 18-19 0.41763470380429624 1108 20-21 0.3298756008150576 1108 22-23 0.4709002176728987 1108 24-25 0.385297500804306 1108 26-27 0.5813025631574078 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0.05698037482891394 2316 74-75 0.18136285430270505 2316 76-77 0.32495267611783163 2316 78-79 0.23491464783026927 2316 80-81 0.41772215971456106 2316 82-83 0.6986967677932405 2316 84-85 1.056687678644952 2316 86-87 1.0657110137156955 2316 88-89 1.0852283492896149 2316 90-91 1.1623385529147967 2316 92-93 1.201317878545133 2316 94-95 1.1270255930591802 2316 96-97 1.056101519164919 2316 98-99 1.1415202579819024 2316 100-101 1.737051063597363 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7495.0 3 1795.0 4 1796.0 5 1896.0 6 2201.0 7 2727.0 8 3368.0 9 4162.0 10 5342.0 11 6587.0 12 7950.0 13 9240.0 14 10793.0 15 12228.0 16 14618.0 17 16924.0 18 15962.0 19 14589.0 20 15313.0 21 16692.0 22 18470.0 23 20542.0 24 23075.0 25 26171.0 26 29935.0 27 34188.0 28 39739.0 29 46042.0 30 54172.0 31 63552.0 32 77024.0 33 94894.0 34 119029.0 35 151526.0 36 190499.0 37 208192.0 38 120413.0 39 9848.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7926839956886 19.152157608415664 14.879091367090828 23.176067028804912 2 15.098806616177693 19.809676547774142 40.8743322160129 24.21718462003526 3 19.227592442856274 24.055106235131795 29.398864783199276 27.31843653881266 4 12.570515954412334 16.39541501669644 37.477737523817424 33.55633150507381 5 13.708332274404736 37.32502598276376 34.44344719379463 14.523194549036866 6 32.308373248608646 36.27141852336214 17.468649459639437 13.951558768389777 7 28.770732887991755 32.30315498943256 20.54343618178548 18.382675940790207 8 26.289783694911605 35.351316077156014 19.591219421523796 18.767680806408585 9 25.83586097692756 17.062931638608486 20.176413474346425 36.92479391011753 10-11 24.78568634203918 26.867800622594633 27.472305907292917 20.874207128073266 12-13 25.586325785361936 23.85137118619927 27.973689648047383 22.58861338039141 14-15 22.440398661677825 25.548440630006336 25.982713338557534 26.028447369758307 16-17 22.114504516009116 28.786190084904582 26.697874343358436 22.401431055727866 18-19 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26.85280546984136 28.55156774240347 22.450979114038933 44-45 22.16177965441663 27.049471576932625 28.524483418275683 22.264265350375062 46-47 22.47993289961452 27.187991732130662 28.096180339306244 22.235895028948573 48-49 22.364276957814056 27.286956620577907 27.949153148659576 22.399613272948464 50-51 22.449730850245743 27.365943361529975 27.924303721287906 22.260022066936376 52-53 22.56322445559459 27.362612156241994 27.847416017820613 22.226747370342803 54-55 22.37277063611673 27.424702762625984 27.91199687618263 22.290529725074652 56-57 22.39652463283797 27.60409372421075 27.72438923871557 22.274992404235714 58-59 22.344396655302216 27.336611098837967 27.83713264255389 22.481859603305928 60-61 22.23172534482799 27.44225516118154 28.060938523040953 22.265080970949523 62-63 22.281327669906158 27.39258406566153 28.098640697259334 22.227447567172977 64-65 22.151928348599384 27.40015013158238 28.023871322661915 22.424050197156323 66-67 22.08501564212947 27.563905452017433 27.840550549479932 22.510528356373165 68-69 22.40930624937748 27.23041884673259 28.006986906569175 22.353287997320752 70-71 22.308053795807755 27.261819054475644 27.830386382459878 22.599740767256726 72-73 22.22609203704638 27.410533465115012 27.812095459047125 22.551279038791478 74-75 22.21727904003476 27.652112876135405 27.67333773428817 22.457270349541663 76-77 22.32782853068468 27.412419618326407 27.715377088954103 22.544374762034813 78-79 22.276316392060014 27.554854753031183 27.679675452809317 22.489153402099486 80-81 22.357056545624413 27.530982014971645 27.496318927149023 22.615642512254922 82-83 22.404632036469632 27.53799560741332 27.633488075474144 22.423884280642906 84-85 22.400078070013027 27.59888589813601 27.552698929812518 22.448337102038444 86-87 22.56434199827078 27.45201776546683 27.49008471316271 22.493555523099687 88-89 22.54036046581197 27.44819177158221 27.298785935529544 22.712661827076282 90-91 22.39506771991676 27.477788228279053 27.47190564333754 22.655238408466644 92-93 22.542918454935624 27.24058401187277 27.644064284090756 22.57243324910085 94-95 22.379577077121407 27.083622308685506 27.678630218393508 22.858170395799583 96-97 22.54677872122229 26.870998429622833 27.802427967554898 22.77979488159998 98-99 22.348616149727725 27.069273907722813 27.46066759214164 23.121442350407822 100-101 22.937163017597616 26.195188763621342 28.0925843483748 22.775063870406242 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2179.0 1 1204.5 2 213.5 3 204.0 4 186.5 5 171.0 6 256.0 7 463.5 8 753.5 9 960.5 10 929.0 11 855.0 12 916.5 13 1027.5 14 1114.0 15 1182.0 16 1304.0 17 1445.5 18 1631.0 19 1832.0 20 2042.5 21 2437.0 22 3054.5 23 3848.0 24 4747.5 25 6015.0 26 7862.5 27 10236.5 28 12501.0 29 14736.5 30 17398.0 31 21001.0 32 25571.0 33 28968.0 34 31393.5 35 34280.5 36 37952.0 37 41624.0 38 43877.5 39 45619.5 40 47390.0 41 48871.5 42 49602.0 43 50911.5 44 53372.5 45 55803.5 46 56449.5 47 56170.0 48 56674.5 49 56417.0 50 54839.5 51 52643.5 52 50938.0 53 49816.5 54 48747.0 55 45618.5 56 41402.5 57 37408.5 58 33983.0 59 29971.5 60 24756.5 61 20794.5 62 17181.5 63 12681.5 64 8714.0 65 6371.0 66 4813.0 67 3656.5 68 2809.5 69 2172.0 70 1628.0 71 1096.0 72 730.0 73 503.5 74 356.5 75 256.0 76 157.0 77 112.5 78 74.0 79 46.5 80 38.0 81 28.5 82 17.0 83 9.5 84 9.5 85 10.5 86 8.5 87 5.5 88 4.0 89 3.5 90 2.5 91 0.5 92 0.5 93 1.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2900621685682817 2 0.24983505549406965 3 0.251369422991096 4 0.25083572994865205 5 0.25130271136079047 6 0.22154932424454082 7 0.22401765456584402 8 0.22481819412950999 9 0.23515849682686132 10-11 0.24243006453015997 12-13 0.23562547823899976 14-15 0.22581886858409234 16-17 0.22081549631118041 18-19 0.20917431682287196 20-21 0.21637917289586517 22-23 0.21240983089268833 24-25 0.21537849844128273 26-27 0.20984143312592685 28-29 0.22248328706881773 30-31 0.233357282808613 32-33 0.22718645700535492 34-35 0.2271531011902022 36-37 0.21674608686254537 38-39 0.20687276557733247 40-41 0.20490477248332042 42-43 0.2150449402897553 44-45 0.21150922388356422 46-47 0.2214492567990826 48-49 0.22418443364160778 50-51 0.23609245965113818 52-53 0.24296375757260394 54-55 0.2256854453234814 56-57 0.20610558182881927 58-59 0.1967659535860503 60-61 0.19943441879827004 62-63 0.20513826318938966 64-65 0.19800011874670193 66-67 0.20206952819533697 68-69 0.20333704917114134 70-71 0.20253650960747543 72-73 0.20550517715606986 74-75 0.20690612139248518 76-77 0.2157787682231157 78-79 0.21637917289586517 80-81 0.21090881921081472 82-83 0.20410423291965454 84-85 0.19319688136470647 86-87 0.1961655489133009 88-89 0.20437107944087646 90-91 0.20333704917114134 92-93 0.20874069122588626 94-95 0.20297013520446114 96-97 0.21147586806841145 98-99 0.2121763401866191 100-101 0.19953448624372827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1498989.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.7223012132111 #Duplication Level Percentage of deduplicated Percentage of total 1 82.2573899400206 54.06144958650595 2 10.777192940738956 14.166038413682763 3 3.0971464371261397 6.106547731266831 4 1.3243234008148739 3.481503258082369 5 0.6833744626935961 2.245647113928241 6 0.41886300484365824 1.6517184342843365 7 0.2877730304397707 1.3239174051320826 8 0.18862363047039432 0.9917423246163743 9 0.14286281885027619 0.845034589138164 >10 0.7534908840870466 8.92586272987303 >50 0.04522702886501255 2.024005500361831 >100 0.021491696775498215 2.551122811707776 >500 0.0016296176539583246 0.7474396339864969 >1k 6.111066202343717E-4 0.877970467433897 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3740 0.2495014973425422 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2863 0.19099539756462522 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2410 0.16077502903623708 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2003 0.1336233955019016 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.671163030549257E-5 2 0.0 0.0 0.0 0.0 6.671163030549257E-5 3 0.0 0.0 0.0 0.0 6.671163030549257E-5 4 0.0 0.0 0.0 0.0 6.671163030549257E-5 5 0.0 0.0 0.0 0.0 1.3342326061098513E-4 6 0.0 0.0 0.0 0.0 2.6684652122197026E-4 7 0.0 0.0 0.0 0.0 2.6684652122197026E-4 8 0.0 0.0 0.0 0.0 2.6684652122197026E-4 9 0.0 0.0 0.0 0.0 2.6684652122197026E-4 10-11 0.0 0.0 0.0 0.0 2.6684652122197026E-4 12-13 0.0 0.0 0.0 0.0 9.673186394296423E-4 14-15 6.671163030549257E-5 0.0 0.0 0.0 0.0011007419000406272 16-17 6.671163030549257E-5 0.0 0.0 3.335581515274628E-5 0.0012675209758043588 18-19 6.671163030549257E-5 0.0 0.0 6.671163030549257E-5 0.001400944236415344 20-21 6.671163030549257E-5 0.0 0.0 6.671163030549257E-5 0.0017011465727900603 22-23 6.671163030549257E-5 0.0 0.0 6.671163030549257E-5 0.0018012140182482993 24-25 6.671163030549257E-5 0.0 0.0 1.3342326061098513E-4 0.0018012140182482993 26-27 6.671163030549257E-5 0.0 0.0 2.001348909164777E-4 0.001867925648553792 28-29 6.671163030549257E-5 0.0 0.0 8.338953788186571E-4 0.001867925648553792 30-31 1.0006744545823885E-4 0.0 0.0 0.0018345698334010455 0.001867925648553792 32-33 1.3342326061098513E-4 0.0 0.0 0.004202832709246032 0.001867925648553792 34-35 1.3342326061098513E-4 0.0 0.0 0.0072382118881459435 0.001867925648553792 36-37 1.3342326061098513E-4 0.0 0.0 0.010106811991282124 0.001867925648553792 38-39 1.3342326061098513E-4 0.0 0.0 0.01327561443079302 0.0019012814637065383 40-41 1.3342326061098513E-4 0.0 0.0 0.01581065638240174 0.0019346372788592845 42-43 1.3342326061098513E-4 0.0 0.0 0.018779323930996156 0.0019679930940120307 44-45 1.3342326061098513E-4 0.0 0.0 0.022248328706881773 0.002001348909164777 46-47 3.0020233637471656E-4 0.0 0.0 0.027585259131321177 0.002001348909164777 48-49 3.3355815152746285E-4 0.0 0.0 0.033322459337593535 0.0020680605394702697 50-51 3.3355815152746285E-4 0.0 0.0 0.03935986188024061 0.002201483800081255 52-53 3.6691396668020915E-4 0.0 0.0 0.0503005692503414 0.0022681954303867477 54-55 4.002697818329554E-4 0.0 0.0 0.06988043274500347 0.0023682628758449863 56-57 4.002697818329554E-4 0.0 0.0 0.10803948527974522 0.0024349745061504786 58-59 4.002697818329554E-4 0.0 0.0 0.14503108428414085 0.002468330321303225 60-61 4.002697818329554E-4 0.0 0.0 0.18235624144006393 0.002468330321303225 62-63 4.002697818329554E-4 0.0 0.0 0.23532527590262503 0.0025350419516087176 64-65 4.66981412138448E-4 0.0 0.0 0.298567901432232 0.0026351093970669566 66-67 4.66981412138448E-4 0.0 0.0 0.3942990909206138 0.002701821027372449 68-69 4.66981412138448E-4 0.0 0.0 0.508776248524839 0.002735176842525195 70-71 4.66981412138448E-4 0.0 0.0 0.616715666359126 0.0028018884728306876 72-73 4.66981412138448E-4 0.0 0.0 0.7284242913056733 0.0030353791788999118 74-75 4.66981412138448E-4 0.0 0.0 0.8434684977674953 0.003268869884969136 76-77 4.66981412138448E-4 0.0 0.0 0.9975390079580304 0.0033355815152746283 78-79 4.66981412138448E-4 0.0 0.0 1.1312291150902376 0.003402293145580121 80-81 5.336930424439405E-4 0.0 0.0 1.2676877548801224 0.003402293145580121 82-83 5.336930424439405E-4 0.0 0.0 1.4225588046343236 0.0034356489607328673 84-85 5.670488575966868E-4 0.0 0.0 1.6256623630993956 0.0034690047758856135 86-87 6.004046727494331E-4 3.335581515274628E-5 0.0 1.8670583973598205 0.0035357164061911063 88-89 6.004046727494331E-4 6.671163030549257E-5 0.0 2.1477142260550277 0.0036024280364965987 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2120 0.0 52.2374 1 GTATCAA 4070 0.0 43.09168 1 ATCAACG 5190 0.0 32.86573 3 TATCAAC 5250 0.0 32.85213 2 TCAACGC 5180 0.0 32.74792 4 CAACGCA 5340 0.0 31.855688 5 AACGCAG 5545 0.0 30.588194 6 ACGCAGA 6505 0.0 25.709703 7 CGCAGAG 6565 0.0 25.474731 8 TACATGG 5005 0.0 25.25188 2 GTACATG 5185 0.0 24.84174 1 ACATGGG 5340 0.0 23.400799 3 GCAGAGT 7410 0.0 21.864416 9 GTGTAGC 975 0.0 21.449123 1 CATGGGA 2700 0.0 20.59052 4 AGAGTAC 6520 0.0 20.583616 10-11 GTATAGG 430 0.0 19.895988 1 GAGTACT 4140 0.0 19.73525 12-13 TATACCG 150 7.7044277E-4 19.006636 5 GTACCGT 125 0.0060244743 19.004093 6 >>END_MODULE