FastQCFastQC Report
Thu 26 May 2016
SRR937267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937267_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498989
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45580.3040716109324351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36600.2441645669181028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26430.17631883889741687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29300.047.1943441
GTATCAA49750.038.970111
ATCAACG61050.031.1218263
TCAACGC61350.030.9696394
CAACGCA62100.030.595615
TATCAAC63400.030.0431772
AACGCAG64600.029.557646
ACGCAGA75300.025.2347627
CGCAGAG76300.024.717258
TATCCCG1401.7800417E-523.7498425
GTACATG49800.023.4730951
GGTAAGG6700.023.3953673
GTAAGGT7550.022.6488554
TACATGG49150.022.2277242
TAAGGTG8550.022.2220755
GCAGAGT86350.021.6204349
GAGTACT51550.021.6096812-13
CAACGTA1100.002876211721.5929269
AGGTAAG7700.021.5907652
TGCACCG2205.642505E-821.5907635
Produced by FastQC (version 0.11.5)