FastQCFastQC Report
Thu 26 May 2016
SRR937266_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937266_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469892
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38130.2594068135618127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28080.19103444334685812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.13538409624652697No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19850.1350439352006814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25650.045.2357941
GTATCAA45100.038.485411
ATCAACG53350.031.8014413
TCAACGC54000.031.420794
CAACGCA54400.030.9287095
TATCAAC55100.030.8776632
AACGCAG57300.029.5121576
TACATGG49900.026.8572522
GTACATG53300.026.3193171
ACATGGG49200.026.0802463
ACGCAGA65200.025.719497
CGCAGAG65500.025.6016888
GTACGGG1151.2722821E-424.8103661
GCAGAGT72200.022.4363619
CATGGGG28400.021.9227774
GAGTACT40900.021.66124212-13
GTGTAGG6300.021.1347581
GTACTTT42850.020.78351814-15
GTGTAGC9450.020.6315481
TATTCCG1456.122047E-419.6671165
Produced by FastQC (version 0.11.5)