##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937266_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1469892 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.409296057125285 31.0 31.0 34.0 30.0 34.0 2 31.524524931083374 33.0 31.0 34.0 30.0 34.0 3 31.560765688907757 33.0 31.0 34.0 28.0 34.0 4 35.187876388197225 37.0 35.0 37.0 33.0 37.0 5 35.199792229633196 37.0 35.0 37.0 33.0 37.0 6 35.228520190599035 37.0 35.0 37.0 32.0 37.0 7 35.1552910009715 37.0 35.0 37.0 32.0 37.0 8 35.137464521202915 37.0 35.0 37.0 32.0 37.0 9 36.72138157089092 39.0 37.0 39.0 32.0 39.0 10-11 36.584430352706185 39.0 37.0 39.0 32.0 39.0 12-13 36.44055923836581 39.0 37.0 39.0 32.0 39.0 14-15 37.55883357416735 40.0 37.0 41.0 32.0 41.0 16-17 37.507825745020725 40.0 37.0 41.0 32.0 41.0 18-19 37.45572872020529 40.0 37.0 41.0 32.0 41.0 20-21 37.37215013075791 40.0 37.0 41.0 32.0 41.0 22-23 37.211869987726985 39.5 37.0 41.0 31.0 41.0 24-25 37.04336781205694 39.0 36.0 41.0 31.0 41.0 26-27 36.87241919814517 39.0 36.0 41.0 30.5 41.0 28-29 36.93768487752841 39.0 36.0 41.0 31.0 41.0 30-31 36.7875860947607 39.0 36.0 41.0 30.0 41.0 32-33 36.59421304422366 39.0 36.0 41.0 30.0 41.0 34-35 36.47698640444332 39.0 35.5 41.0 30.0 41.0 36-37 36.46727242545711 39.0 36.0 41.0 30.0 41.0 38-39 36.36450807270194 39.0 35.0 41.0 30.0 41.0 40-41 36.184823782971804 39.0 35.0 41.0 29.5 41.0 42-43 35.94881052485489 39.0 35.0 41.0 28.5 41.0 44-45 35.73821886233819 39.0 35.0 40.0 27.5 41.0 46-47 35.44947485937742 39.0 35.0 40.0 26.5 41.0 48-49 35.15608425653041 38.5 34.5 40.0 26.0 41.0 50-51 34.298069858193664 37.5 33.5 39.5 24.5 40.5 52-53 34.39697032162907 38.0 33.5 39.5 24.0 40.5 54-55 34.70706249166606 38.0 34.0 40.0 24.5 41.0 56-57 34.482289515148054 38.0 34.0 40.0 23.5 41.0 58-59 34.218547349056934 38.0 34.0 40.0 23.0 41.0 60-61 33.916155404614756 38.0 33.0 40.0 22.5 41.0 62-63 33.559758131889964 37.0 33.0 40.0 22.0 41.0 64-65 33.18103268811586 36.5 32.5 39.5 20.5 41.0 66-67 32.79157074125174 36.0 32.0 39.0 19.5 41.0 68-69 32.29725993474351 35.5 31.0 39.0 18.0 40.0 70-71 31.8435381647087 35.0 31.0 38.5 14.5 40.0 72-73 31.445566749121703 35.0 31.0 37.5 12.0 40.0 74-75 30.96948313209406 35.0 30.5 37.0 8.0 39.0 76-77 30.514594269510958 34.5 30.0 36.5 7.0 39.0 78-79 30.02833881672939 34.0 29.5 36.0 3.5 38.5 80-81 29.531966634283336 34.0 29.0 35.5 2.0 37.0 82-83 29.027164240638086 34.0 29.0 35.0 2.0 37.0 84-85 28.564066951857686 34.0 28.5 35.0 2.0 36.5 86-87 28.095930177183085 34.0 27.0 35.0 2.0 36.0 88-89 27.64718326244377 33.0 26.0 35.0 2.0 35.5 90-91 27.185928966209765 33.0 26.0 35.0 2.0 35.0 92-93 26.685353753881238 33.0 25.0 35.0 2.0 35.0 94-95 26.098877332484292 32.0 24.5 35.0 2.0 35.0 96-97 25.384492534145366 32.0 21.5 34.0 2.0 35.0 98-99 24.52974470233187 32.0 10.0 34.0 2.0 35.0 100-101 22.540421677238875 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14429640992248594 1101 2 0.1816643920476082 1101 3 0.08684822362636524 1101 4 0.16077221950049392 1101 5 0.2541494143409224 1101 6 0.31401164938549186 1101 7 0.27712693301666746 1101 8 0.34856294580254854 1101 9 0.48369117229420766 1101 10-11 0.5099887508456717 1101 12-13 0.30696912633418805 1101 14-15 0.4468285411882107 1101 16-17 0.5464473479854561 1101 18-19 0.5161397688767622 1101 20-21 0.6176890073691723 1101 22-23 0.7833953332359229 1101 24-25 0.7695887368874352 1101 26-27 0.6585874845602504 1101 28-29 0.5795454194387943 1101 30-31 0.5727828017635872 1101 32-33 0.6237624228189134 1101 34-35 0.5875010561952578 1101 36-37 0.577269368658861 1101 38-39 0.652926757413951 1101 40-41 0.656320618813929 1101 42-43 0.7959582868968127 1101 44-45 0.7108246878460989 1101 46-47 0.7191159656226418 1101 48-49 0.817704458311745 1101 50-51 0.6202867996708008 1101 52-53 0.6760543981634868 1101 54-55 0.6366660227623342 1101 56-57 0.557135090777912 1101 58-59 0.7565570673184325 1101 60-61 0.6622824249714583 1101 62-63 0.710653736439113 1101 64-65 0.780005200903851 1101 66-67 0.7808839948400745 1101 68-69 0.7341146186592766 1101 70-71 0.7419461688768934 1101 72-73 0.5025397439428083 1101 74-75 0.5064757990700919 1101 76-77 0.6838363158319005 1101 78-79 0.7116076731624759 1101 80-81 0.6261936505610599 1101 82-83 0.7935913627904085 1101 84-85 0.7080516434713005 1101 86-87 0.7405503056921816 1101 88-89 0.8249365636690982 1101 90-91 0.8659370230085166 1101 92-93 0.8799509715354326 1101 94-95 0.9955208683154488 1101 96-97 1.1268828307503291 1101 98-99 1.1193988853612886 1101 100-101 1.4415236414056132 1102 1 0.29215200023155674 1102 2 0.3629823160257679 1102 3 0.469038466188767 1102 4 0.4036758355260943 1102 5 0.4286410839394179 1102 6 0.453232731542478 1102 7 0.5063168333988486 1102 8 0.5933490349718227 1102 9 0.7214090712112835 1102 10-11 0.6442429184561433 1102 12-13 0.6652243445825192 1102 14-15 0.8812212676898241 1102 16-17 0.9294759320822763 1102 18-19 0.9174735488115076 1102 20-21 0.867056455909605 1102 22-23 1.014383125595181 1102 24-25 0.952632743191252 1102 26-27 1.0691326595782087 1102 28-29 1.0043705942639676 1102 30-31 0.9397469885072312 1102 32-33 0.9346432718114102 1102 34-35 0.9504072747182946 1102 36-37 0.9970117214129317 1102 38-39 1.1138170043138444 1102 40-41 0.9664542136821908 1102 42-43 1.1326115715859402 1102 44-45 1.122795694096915 1102 46-47 1.266979748979189 1102 48-49 1.1018294649384686 1102 50-51 1.0045397624213521 1102 52-53 1.1787797913092817 1102 54-55 1.005043992139619 1102 56-57 1.2709991729397316 1102 58-59 1.3681622780966336 1102 60-61 1.2520652128245402 1102 62-63 1.0779740797759914 1102 64-65 1.2060174037251556 1102 66-67 1.1520856739328664 1102 68-69 1.1614890361095007 1102 70-71 1.0813190633841714 1102 72-73 1.0833058420762391 1102 74-75 1.1920251910977804 1102 76-77 1.288368275946219 1102 78-79 1.1898951334623433 1102 80-81 1.211364234628956 1102 82-83 1.3929677272942698 1102 84-85 1.5086297352623497 1102 86-87 1.6093037094214644 1102 88-89 1.4989930378634106 1102 90-91 1.4903108573065431 1102 92-93 1.7176975461186679 1102 94-95 1.490847939590143 1102 96-97 1.9542982134379407 1102 98-99 2.0487777077793936 1102 100-101 2.6118149283405927 1103 1 0.1951832909747111 1103 2 0.1801161708714858 1103 3 0.1620978079769202 1103 4 0.09809822105017219 1103 5 0.11210973393349377 1103 6 0.20841624859040309 1103 7 0.1807775421815876 1103 8 0.35049810059546616 1103 9 0.20866362456752796 1103 10-11 0.40297775706386574 1103 12-13 0.30061903463822404 1103 14-15 0.19786620467374405 1103 16-17 0.2531691879789193 1103 18-19 0.29875194555600615 1103 20-21 0.04776839933340682 1103 22-23 -0.011552238475779575 1103 24-25 0.058845950887516096 1103 26-27 0.31334219298265253 1103 28-29 -0.009790738753295614 1103 30-31 0.009763483311864718 1103 32-33 0.11547571620317854 1103 34-35 0.19326364151533681 1103 36-37 0.14816339459850525 1103 38-39 -0.0015305718388134437 1103 40-41 0.1395963258073536 1103 42-43 0.08611153207741751 1103 44-45 -0.02126271924116452 1103 46-47 0.009305713974647745 1103 48-49 0.026389438862857162 1103 50-51 -0.08006426349623297 1103 52-53 0.053876093312361206 1103 54-55 0.10015530737805278 1103 56-57 0.037038148088868184 1103 58-59 0.38119860617533163 1103 60-61 0.11695449021381421 1103 62-63 -0.03950152371656657 1103 64-65 0.025294101603542174 1103 66-67 0.0033632805070809013 1103 68-69 0.32373963861434163 1103 70-71 0.41662041457535537 1103 72-73 0.49559002681805353 1103 74-75 0.2283868520357082 1103 76-77 0.3884981601862698 1103 78-79 0.3984615670287752 1103 80-81 0.2955603351386138 1103 82-83 0.34609630775335987 1103 84-85 0.5961480079501165 1103 86-87 0.5417713124399128 1103 88-89 0.6330824653850406 1103 90-91 0.8091509861042994 1103 92-93 0.5570667926913053 1103 94-95 0.503192374815935 1103 96-97 0.8579374414415746 1103 98-99 0.9240266114181246 1103 100-101 1.2021363301026717 1104 1 0.2260934751457171 1104 2 0.20630727887105138 1104 3 0.1633178044387904 1104 4 0.3010086160034433 1104 5 0.33231462558764235 1104 6 0.23913117686797847 1104 7 0.3232207156591542 1104 8 0.3759350033006257 1104 9 0.4941023339778283 1104 10-11 0.4466762612625743 1104 12-13 0.5535111743417005 1104 14-15 0.6115574538221509 1104 16-17 0.6991854031575002 1104 18-19 0.6964206842876237 1104 20-21 0.7493703445526094 1104 22-23 0.7035397717471668 1104 24-25 0.5399435336666087 1104 26-27 0.6761161158288118 1104 28-29 0.5115907225700411 1104 30-31 0.5587306031604058 1104 32-33 0.3418398726789036 1104 34-35 0.5021676396032362 1104 36-37 0.5925582801173874 1104 38-39 0.5749897423020371 1104 40-41 0.524438634324838 1104 42-43 0.7193360396561701 1104 44-45 0.7051482515993683 1104 46-47 0.7071156956441129 1104 48-49 0.7626478936782917 1104 50-51 0.7795112266666351 1104 52-53 0.60367834378944 1104 54-55 0.690867196250629 1104 56-57 0.5899296761473778 1104 58-59 0.5917693838489697 1104 60-61 0.8586128290990018 1104 62-63 0.5917043202217585 1104 64-65 0.7217782251821845 1104 66-67 0.8426707384353094 1104 68-69 1.020819645540044 1104 70-71 1.0604588443227598 1104 72-73 1.0596833619580899 1104 74-75 0.8472238232451055 1104 76-77 0.8232184690575721 1104 78-79 0.7589712272552909 1104 80-81 0.6548667015158323 1104 82-83 0.7677067124929522 1104 84-85 0.8666178830239843 1104 86-87 0.8697699254173266 1104 88-89 0.7325366428260658 1104 90-91 0.9146750341024301 1104 92-93 1.1423512734705987 1104 94-95 1.2170752099879678 1104 96-97 1.1627890563724854 1104 98-99 1.0689060138504054 1104 100-101 1.4039034194630595 1105 1 0.23371311649146875 1105 2 0.3963568397617365 1105 3 0.30324147870502927 1105 4 0.3528375172178926 1105 5 0.30468512022441985 1105 6 0.4166219527630304 1105 7 0.4150791554141833 1105 8 0.3602319512523309 1105 9 0.34509636116363396 1105 10-11 0.37475611342716775 1105 12-13 0.34054427231207995 1105 14-15 0.3796611811899808 1105 16-17 0.25160360360752776 1105 18-19 0.42459855232205257 1105 20-21 0.4047386532303534 1105 22-23 0.38741651790568454 1105 24-25 0.32696503740307037 1105 26-27 0.40580101130679935 1105 28-29 0.44969267512821887 1105 30-31 0.4058889456130501 1105 32-33 0.4100324743090482 1105 34-35 0.4285763613211131 1105 36-37 0.4296963059047485 1105 38-39 0.47580107111718917 1105 40-41 0.3107441941167508 1105 42-43 0.41741839795902536 1105 44-45 0.4851570275425843 1105 46-47 0.5172882089261961 1105 48-49 0.5539559194745678 1105 50-51 0.3673794662249108 1105 52-53 0.4291753127891411 1105 54-55 0.5135844476680305 1105 56-57 0.6025388514673509 1105 58-59 0.5844366997927324 1105 60-61 0.4927075022620073 1105 62-63 0.5916530380332574 1105 64-65 0.6616431701942957 1105 66-67 0.6710098555076485 1105 68-69 0.7590316541340485 1105 70-71 0.9034958825489454 1105 72-73 0.7981250255701191 1105 74-75 0.745370039020937 1105 76-77 0.8614620496424017 1105 78-79 0.8233276982202078 1105 80-81 0.8810817995272409 1105 82-83 0.9314873067777079 1105 84-85 0.8734578121386214 1105 86-87 0.8349709471368918 1105 88-89 0.9047723321969201 1105 90-91 1.0160534547358857 1105 92-93 1.0834463221084114 1105 94-95 1.3558459268772864 1105 96-97 1.3926993705137924 1105 98-99 1.6350486657370986 1105 100-101 1.9592863993060377 1106 1 0.035339188469937 1106 2 0.1321605780367925 1106 3 -0.012142468917083704 1106 4 0.02841628279213637 1106 5 0.008177588390594792 1106 6 0.035929215945451176 1106 7 0.005572185555223541 1106 8 -0.0942330075590263 1106 9 -0.0034244570352157666 1106 10-11 -0.3029730055515856 1106 12-13 -0.27712869486949643 1106 14-15 -0.26872622509043254 1106 16-17 -0.1535439490604773 1106 18-19 -0.019540461069396997 1106 20-21 0.026633056783133213 1106 22-23 0.11510748655845049 1106 24-25 0.05127113551565543 1106 26-27 -0.01175590578134944 1106 28-29 0.16760002694732634 1106 30-31 0.17892214781891624 1106 32-33 0.32908227990229477 1106 34-35 0.23268608805512514 1106 36-37 0.21334146675292232 1106 38-39 0.03161933849843024 1106 40-41 0.1564384615162666 1106 42-43 0.25606559196828016 1106 44-45 0.4004180717192867 1106 46-47 0.5187996014816321 1106 48-49 0.7322442719744444 1106 50-51 0.5347433472324497 1106 52-53 0.34424674756121476 1106 54-55 0.506618015385996 1106 56-57 0.4344613080742121 1106 58-59 0.5078219358450227 1106 60-61 0.41557508179516134 1106 62-63 0.49392195442693776 1106 64-65 0.6236013556094449 1106 66-67 0.7018995119719591 1106 68-69 0.3677230751080458 1106 70-71 0.6436284263761323 1106 72-73 0.6962846721199192 1106 74-75 0.6144004267835932 1106 76-77 0.7430032314344182 1106 78-79 0.6767038878478893 1106 80-81 0.9237845787899133 1106 82-83 0.8456467888470556 1106 84-85 0.6959575951158072 1106 86-87 0.9875048598069149 1106 88-89 0.761841793203633 1106 90-91 0.940519692839608 1106 92-93 1.0387780014994696 1106 94-95 1.2255241848465914 1106 96-97 1.1917501812653342 1106 98-99 1.484353735846888 1106 100-101 1.4637004148677164 1107 1 -0.04211465964728234 1107 2 -0.17228215477808106 1107 3 -0.11610544350842034 1107 4 -0.07682288481379373 1107 5 -0.10469163231851297 1107 6 -0.15830781262545912 1107 7 -0.14244677889885082 1107 8 -0.051633910606753375 1107 9 -0.04724689228253709 1107 10-11 0.06012875333603063 1107 12-13 0.09305220831375749 1107 14-15 0.0893895719203428 1107 16-17 0.0847741414837273 1107 18-19 -0.07180067795444955 1107 20-21 -0.0801373165654411 1107 22-23 0.10699835285633696 1107 24-25 0.01516323057283131 1107 26-27 -0.002052773122777296 1107 28-29 0.20727539958439678 1107 30-31 0.08879152438168347 1107 32-33 0.02607949938264653 1107 34-35 0.08994807805790828 1107 36-37 0.10959126628897309 1107 38-39 0.16630852338379043 1107 40-41 0.03451662777372633 1107 42-43 0.10594373247600686 1107 44-45 0.1984988129502625 1107 46-47 0.1523291447852344 1107 48-49 0.19456224506371456 1107 50-51 0.2617130325661705 1107 52-53 0.13034708569590236 1107 54-55 0.11755758708206798 1107 56-57 0.20278009883050885 1107 58-59 0.20432968448243116 1107 60-61 0.3899662925522307 1107 62-63 0.11796565669675374 1107 64-65 0.2143014095935385 1107 66-67 0.32760158753612956 1107 68-69 0.238425071616021 1107 70-71 0.3823862952919086 1107 72-73 0.33723668952131547 1107 74-75 0.19923247752412365 1107 76-77 0.21597075288688217 1107 78-79 0.3371611037594171 1107 80-81 0.40047948734447303 1107 82-83 0.3779570596032613 1107 84-85 0.3640473487540845 1107 86-87 0.45229774384501553 1107 88-89 0.5420571874795108 1107 90-91 0.7243002960610383 1107 92-93 0.9498619072373664 1107 94-95 0.9109170828889361 1107 96-97 0.9943707815052143 1107 98-99 1.1007041400499666 1107 100-101 1.3103115012580808 1108 1 0.059895854315616504 1108 2 0.011753132837821312 1108 3 -0.10908135213723824 1108 4 0.011699770354148598 1108 5 -0.15229486155838146 1108 6 -0.04508570260069433 1108 7 -0.11014286669487205 1108 8 -0.044032011816440786 1108 9 -0.14835338282952648 1108 10-11 0.07820090634454857 1108 12-13 -0.029441685263925876 1108 14-15 0.09311163247967613 1108 16-17 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70-71 -2.1694999733118543 2316 72-73 -2.150680065713768 2316 74-75 -1.9886800565778593 2316 76-77 -2.0696107609622025 2316 78-79 -1.9271838391974967 2316 80-81 -2.182737639199569 2316 82-83 -2.299965459488572 2316 84-85 -2.5050852134184325 2316 86-87 -2.5272470974026895 2316 88-89 -2.7036926591275297 2316 90-91 -2.8981005035421745 2316 92-93 -2.9548648573093352 2316 94-95 -3.1618688197325397 2316 96-97 -2.9836557081150694 2316 98-99 -3.350732728633094 2316 100-101 -4.026939445607404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6705.0 3 1683.0 4 1547.0 5 1679.0 6 1815.0 7 2220.0 8 2698.0 9 3298.0 10 4072.0 11 5040.0 12 6116.0 13 7384.0 14 8214.0 15 9535.0 16 11242.0 17 13421.0 18 12048.0 19 10068.0 20 10297.0 21 11048.0 22 12153.0 23 13366.0 24 15291.0 25 17159.0 26 19843.0 27 23360.0 28 27512.0 29 32759.0 30 39729.0 31 48704.0 32 60993.0 33 78178.0 34 104332.0 35 142473.0 36 203895.0 37 270091.0 38 204658.0 39 25264.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.710081667616556 19.2009337342202 14.961998791878969 23.126985806284278 2 15.056900362961548 19.849493764156865 40.760718281800074 24.332887591081516 3 19.257995851981224 24.01047920532693 29.381344831350287 27.350180111341555 4 12.520476745717197 16.396543054553938 37.53461687249733 33.54836332723153 5 13.705853897833965 37.350213860799784 34.44342633042978 14.500505910936479 6 32.25203654984016 36.24632623384344 17.454914341851314 14.046722874465084 7 28.71416038451602 32.3184186362933 20.575506122412808 18.391914856777873 8 26.28157146655214 35.32295778200435 19.674213141910545 18.721257609532962 9 25.801409534638164 17.08435326572639 20.120530957887784 36.99370624174767 10-11 24.76638301832697 26.90763764149036 27.444843346820864 20.881135993361806 12-13 25.64792351893091 23.853668529993957 27.953318078115213 22.545089872959917 14-15 22.440551422116602 25.5326205366699 25.964927350095234 26.061900691118268 16-17 22.091807275074835 28.786883077839715 26.710905113439985 22.410404533645465 18-19 22.01711066855399 27.43180801095039 28.88114660088979 21.66993471960583 20-21 22.646518826315706 26.536760197552173 29.125346655256056 21.691374320876065 22-23 22.208827621371103 26.468003945442735 29.058887845950505 22.26428058723566 24-25 22.14739941520069 26.699189596297636 29.125288821336316 22.02812216716536 26-27 21.961567206269805 26.902887452173708 28.93528545143555 22.20025989012094 28-29 21.88548826064654 27.226983556213753 28.88271212552425 22.004816057615457 30-31 22.117727506384675 26.914242899820973 28.7725815622371 22.19544803155726 32-33 21.78686211799 26.883407850116114 28.835728243763786 22.494001788130102 34-35 22.31107938614926 27.00086277806355 28.689126167059875 21.998931668727312 36-37 22.376542736523962 26.71909065410018 28.696424487748253 22.207942121627607 38-39 21.96307389038997 27.01524512436848 28.725022016342532 22.29665896889902 40-41 22.360037828765197 26.80396350035358 28.790723432534442 22.045275238346782 42-43 22.089202461975134 26.921573365570723 28.55691434449179 22.432309827962346 44-45 22.11753478583175 27.12536532578966 28.536498496093248 22.220601392285342 46-47 22.513503371113575 27.17750486809864 28.072185219862867 22.23680654092492 48-49 22.300367623874926 27.356670149179962 27.95880976444462 22.384152462500488 50-51 22.39570550474227 27.421755267219705 27.99084815463237 22.191691073405654 52-53 22.590225481879898 27.312948502197 27.888006393244545 22.208819622678565 54-55 22.370730822745678 27.478132172957455 27.892753399900876 22.25838360439599 56-57 22.36282764228418 27.63281026890233 27.728230961697452 22.276131127116038 58-59 22.309575326233684 27.32851836692825 27.927191382816414 22.434714924021655 60-61 22.19440689223438 27.49100081685286 28.037171065074585 22.277421225838175 62-63 22.21097550294136 27.40511943845472 28.231173929150437 22.15273112945349 64-65 22.1177684577424 27.402358013630636 28.10914187782999 22.37073165079697 66-67 22.045878578353612 27.599936615794597 27.880633228658517 22.473551577193273 68-69 22.404199339116605 27.274270017586982 28.05690944152729 22.264621201769124 70-71 22.258067154606092 27.34595350625238 27.87481204698723 22.5211672921543 72-73 22.220851460894565 27.496525574430407 27.800073475836466 22.482549488838558 74-75 22.167336249780174 27.697531562652937 27.709903059270037 22.42522912829685 76-77 22.282916721338296 27.507399840160048 27.792930575270475 22.41675286323119 78-79 22.2785565063883 27.62717813970766 27.657338963353745 22.43692639055029 80-81 22.303119161498284 27.6593235738975 27.528213957806358 22.509343306797867 82-83 22.318003615118066 27.615405692038834 27.667240105454578 22.39935058738853 84-85 22.30392315553555 27.72202629976443 27.568578634346448 22.405471910353576 86-87 22.461199265985016 27.6211133242121 27.518916901938802 22.398770507864082 88-89 22.409492968430264 27.663584650599017 27.290750782658062 22.636171598312654 90-91 22.289062601172184 27.681322703395704 27.483766443994146 22.545848251437967 92-93 22.401261411263487 27.449428197258058 27.68333588346327 22.465974508015186 94-95 22.231983275204332 27.281351750600173 27.77348601922261 22.713178954972886 96-97 22.41072685123817 27.13291035934161 27.743075161085756 22.713287628334463 98-99 22.150746726946 27.258234307624306 27.639410443033334 22.951608522396356 100-101 22.278390173141542 27.0341175151662 27.99692365463559 22.690568657056666 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1837.0 1 1024.0 2 208.5 3 212.5 4 170.5 5 159.5 6 268.0 7 500.0 8 821.0 9 975.5 10 909.5 11 855.5 12 924.0 13 1044.0 14 1143.5 15 1237.0 16 1323.0 17 1426.5 18 1610.0 19 1801.5 20 2090.5 21 2514.0 22 3050.0 23 3789.5 24 4657.5 25 5818.5 26 7617.0 27 10090.0 28 12436.0 29 14556.5 30 17152.5 31 20763.5 32 25396.5 33 28484.5 34 30783.5 35 33732.5 36 37446.5 37 41066.5 38 42988.5 39 44716.0 40 46501.5 41 48152.0 42 49099.0 43 50320.0 44 52641.5 45 54972.5 46 55421.0 47 55356.0 48 56171.5 49 55751.5 50 53778.0 51 51667.5 52 50091.5 53 49118.5 54 48252.0 55 44904.0 56 40302.5 57 36219.0 58 33077.0 59 29181.5 60 23857.0 61 19887.0 62 16309.0 63 11754.0 64 7953.0 65 5812.5 66 4289.0 67 3260.5 68 2549.0 69 1970.5 70 1486.5 71 956.0 72 620.5 73 450.0 74 308.5 75 211.5 76 148.5 77 105.5 78 65.5 79 42.0 80 36.0 81 24.5 82 20.0 83 16.0 84 12.0 85 11.0 86 10.0 87 6.5 88 4.5 89 5.5 90 5.0 91 5.0 92 4.5 93 1.5 94 3.0 95 4.5 96 5.0 97 5.0 98 5.5 99 5.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3270308294759071 2 0.28383037665352284 3 0.28110908828675846 4 0.28607543955610343 5 0.28702789048447097 6 0.20906297877667204 7 0.1976335676362617 8 0.20287204774228312 9 0.1961368590345413 10-11 0.21964198730246848 12-13 0.20896093046291836 14-15 0.20368843425231242 16-17 0.20362040204314333 18-19 0.19814380920503005 20-21 0.19297336130817774 22-23 0.1961368590345413 24-25 0.18518367335831476 26-27 0.18596604376375953 28-29 0.19896019571505932 30-31 0.21076378400589976 32-33 0.2038585147752352 34-35 0.21215844429386652 36-37 0.19154468491562646 38-39 0.19049018567350526 40-41 0.18739472015631078 42-43 0.19447006990989812 44-45 0.19589874630244944 46-47 0.2002528076892724 48-49 0.20678389976950687 50-51 0.20981133307753225 52-53 0.22749970746150056 54-55 0.20484498180818725 56-57 0.1840271258024399 58-59 0.1857619471362522 60-61 0.1820201756319512 62-63 0.1903201051505825 64-65 0.18249640109613496 66-67 0.18025133819355435 68-69 0.17780217866346643 70-71 0.18844921939843198 72-73 0.18661234975086605 74-75 0.19117050776519634 76-77 0.19045616956892072 78-79 0.18756480067923356 80-81 0.19042215346433614 82-83 0.18504760893997654 84-85 0.17800627529097376 86-87 0.17821037191848108 88-89 0.17800627529097376 90-91 0.184707447894131 92-93 0.180047241566047 94-95 0.17753004982679 96-97 0.17576121238839315 98-99 0.18521768946289932 100-101 0.1746046648325183 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1469892.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.55084062149542 #Duplication Level Percentage of deduplicated Percentage of total 1 81.95491997893227 53.722138976863995 2 10.993814884393077 14.413076146181497 3 3.1375043309146684 6.169981390351173 4 1.3528619833710203 3.547249610193358 5 0.6903519527969365 2.262657541526506 6 0.43893082183146714 1.7263370607441906 7 0.2677294291927117 1.2284922399886773 8 0.1931715028188084 1.013004351511237 9 0.1501426262057411 0.8857777824814765 >10 0.7503521787439462 8.824338802049462 >50 0.04659772326290811 2.071847644591127 >100 0.021749504490665556 2.6563236621987203 >500 0.0012487220304554852 0.6111823483654012 >1k 6.243610152277426E-4 0.8675924429530736 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3813 0.2594068135618127 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2808 0.19103444334685812 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1990 0.13538409624652697 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1985 0.1350439352006814 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.803220916910902E-5 2 0.0 0.0 0.0 0.0 1.3606441833821805E-4 3 0.0 0.0 0.0 0.0 1.3606441833821805E-4 4 0.0 0.0 0.0 0.0 1.3606441833821805E-4 5 0.0 0.0 0.0 0.0 2.0409662750732707E-4 6 0.0 0.0 0.0 0.0 2.0409662750732707E-4 7 0.0 0.0 0.0 0.0 2.0409662750732707E-4 8 0.0 0.0 0.0 0.0 2.0409662750732707E-4 9 0.0 0.0 0.0 0.0 2.0409662750732707E-4 10-11 1.0204831375366354E-4 0.0 0.0 0.0 2.721288366764361E-4 12-13 2.0409662750732707E-4 0.0 0.0 0.0 3.7417715043009964E-4 14-15 2.0409662750732707E-4 0.0 0.0 0.0 4.4220935959920864E-4 16-17 2.0409662750732707E-4 0.0 0.0 3.401610458455451E-5 4.7622546418376314E-4 18-19 2.0409662750732707E-4 0.0 0.0 6.803220916910902E-5 6.122898825219812E-4 20-21 2.0409662750732707E-4 0.0 0.0 6.803220916910902E-5 7.483543008601993E-4 22-23 2.0409662750732707E-4 0.0 0.0 6.803220916910902E-5 8.163865100293083E-4 24-25 2.0409662750732707E-4 0.0 0.0 2.0409662750732707E-4 8.844187191984173E-4 26-27 2.0409662750732707E-4 0.0 0.0 2.0409662750732707E-4 8.844187191984173E-4 28-29 2.0409662750732707E-4 0.0 0.0 6.122898825219812E-4 8.844187191984173E-4 30-31 2.0409662750732707E-4 0.0 0.0 0.002245062902580598 8.844187191984173E-4 32-33 2.0409662750732707E-4 0.0 0.0 0.004660206328083968 8.844187191984173E-4 34-35 2.0409662750732707E-4 0.0 0.0 0.008095832891123974 0.0010204831375366353 36-37 2.0409662750732707E-4 0.0 0.0 0.011565475558748532 0.0010204831375366353 38-39 3.401610458455451E-4 0.0 0.0 0.015035118226373094 0.0010204831375366353 40-41 3.401610458455451E-4 0.0 0.0 0.017280181128953694 0.0010204831375366353 42-43 3.401610458455451E-4 0.0 0.0 0.01932114740402696 0.0010204831375366353 44-45 4.0819325501465414E-4 0.0 0.0 0.022348580712052313 0.0010885153467057444 46-47 4.0819325501465414E-4 0.0 0.0 0.0275190286089046 0.001190563660459408 48-49 4.0819325501465414E-4 0.0 0.0 0.0331997180745252 0.0012926119742130715 50-51 4.0819325501465414E-4 0.0 0.0 0.03826811765762382 0.0014286763925512895 52-53 4.0819325501465414E-4 0.0 0.0 0.04857499734674384 0.0016667891246431711 54-55 4.0819325501465414E-4 0.0 0.0 0.06636542004446586 0.0018028535429813891 56-57 4.0819325501465414E-4 0.0 0.0 0.09976923474649838 0.0019049018567350528 58-59 4.0819325501465414E-4 0.0 0.0 0.13500991909609686 0.001972934065904162 60-61 4.0819325501465414E-4 0.0 0.0 0.17120305437406286 0.0020069501704887164 62-63 4.0819325501465414E-4 0.0 0.0 0.2221591790417255 0.0021089984842423797 64-65 4.0819325501465414E-4 0.0 0.0 0.2834221833985082 0.0021089984842423797 66-67 4.7622546418376314E-4 0.0 0.0 0.3779529380389852 0.002143014588826934 68-69 4.7622546418376314E-4 0.0 0.0 0.49119255020096714 0.0021770306934114888 70-71 4.7622546418376314E-4 0.0 0.0 0.5913019459933111 0.002245062902580598 72-73 4.7622546418376314E-4 0.0 0.0 0.6916834706223314 0.002347111216334261 74-75 4.7622546418376314E-4 0.0 0.0 0.8122705613745771 0.0023811273209188156 76-77 4.7622546418376314E-4 0.0 0.0 0.9593221814936064 0.0023811273209188156 78-79 5.442576733528722E-4 0.0 0.0 1.086406348221502 0.0024831756346724793 80-81 6.803220916910902E-4 0.0 0.0 1.2225047826643045 0.002585223948426143 82-83 6.803220916910902E-4 0.0 0.0 1.3745567701572634 0.00278932057593347 84-85 7.143381962756447E-4 0.0 0.0 1.5764083347620097 0.00278932057593347 86-87 7.483543008601993E-4 0.0 0.0 1.8190111926590524 0.0028913688896871335 88-89 7.483543008601993E-4 0.0 0.0 2.105834986516016 0.002993417203440797 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2565 0.0 45.235794 1 GTATCAA 4510 0.0 38.48541 1 ATCAACG 5335 0.0 31.801441 3 TCAACGC 5400 0.0 31.42079 4 CAACGCA 5440 0.0 30.928709 5 TATCAAC 5510 0.0 30.877663 2 AACGCAG 5730 0.0 29.512157 6 TACATGG 4990 0.0 26.857252 2 GTACATG 5330 0.0 26.319317 1 ACATGGG 4920 0.0 26.080246 3 ACGCAGA 6520 0.0 25.71949 7 CGCAGAG 6550 0.0 25.601688 8 GTACGGG 115 1.2722821E-4 24.810366 1 GCAGAGT 7220 0.0 22.436361 9 CATGGGG 2840 0.0 21.922777 4 GAGTACT 4090 0.0 21.661242 12-13 GTGTAGG 630 0.0 21.134758 1 GTACTTT 4285 0.0 20.783518 14-15 GTGTAGC 945 0.0 20.631548 1 TATTCCG 145 6.122047E-4 19.667116 5 >>END_MODULE