FastQCFastQC Report
Thu 26 May 2016
SRR937266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469892
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46370.3154653539171585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38890.264577261458665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26490.18021732208896982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24900.051.5359041
GTATCAA45550.040.1714741
ATCAACG57300.031.087493
TCAACGC57750.030.845254
CAACGCA59200.030.2502275
TATCAAC59650.029.9423872
AACGCAG62000.028.881146
TAGCCCG700.00839086727.1438185
ACGCAGA70350.025.2523467
CGCAGAG70350.025.1173088
TGGACCG1601.8595711E-623.7508415
GCAGAGT80750.021.8823859
TACATGG48050.021.6500912
TGGTATC12950.021.6416932
GTACATG49150.021.5638771
GGTAAGG8100.021.111863
GTAAGGT8600.020.9891154
GAGTACT50250.020.79605112-13
ACATGGG47950.020.7045923
GTGGTAT14650.020.114051
Produced by FastQC (version 0.11.5)