##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937266_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1469892 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09644858261695 31.0 31.0 34.0 31.0 34.0 2 32.32317816547066 34.0 31.0 34.0 31.0 34.0 3 32.41572101895922 34.0 31.0 34.0 31.0 34.0 4 36.047295991814366 37.0 35.0 37.0 35.0 37.0 5 35.894353462703386 37.0 35.0 37.0 35.0 37.0 6 35.98084281022007 37.0 35.0 37.0 35.0 37.0 7 35.94419317881858 37.0 35.0 37.0 35.0 37.0 8 35.90962873462812 37.0 35.0 37.0 35.0 37.0 9 37.61106394211276 39.0 37.0 39.0 35.0 39.0 10-11 37.52753841778852 39.0 37.0 39.0 35.0 39.0 12-13 37.44515005184054 39.0 37.0 39.0 34.5 39.0 14-15 38.66839332413538 40.0 38.0 41.0 34.0 41.0 16-17 38.636550168311686 40.0 38.0 41.0 34.0 41.0 18-19 38.62551058172981 40.0 38.0 41.0 34.0 41.0 20-21 38.52725234234896 40.0 38.0 41.0 34.0 41.0 22-23 38.39825408941609 40.0 38.0 41.0 34.0 41.0 24-25 38.24896965219213 40.0 38.0 41.0 34.0 41.0 26-27 38.033693631913096 40.0 37.0 41.0 33.0 41.0 28-29 38.03319223453151 40.0 37.0 41.0 33.0 41.0 30-31 38.17233068824104 40.0 38.0 41.0 34.0 41.0 32-33 38.14810679968325 40.0 38.0 41.0 34.0 41.0 34-35 38.043865807828055 40.0 37.5 41.0 33.0 41.0 36-37 37.89047494645865 40.0 37.0 41.0 33.0 41.0 38-39 37.75053405284198 40.0 37.0 41.0 33.0 41.0 40-41 37.600206001529365 40.0 37.0 41.0 33.0 41.0 42-43 37.41643705796072 40.0 37.0 41.0 32.5 41.0 44-45 37.17132823363893 40.0 36.5 41.0 31.5 41.0 46-47 36.90156079494275 39.5 36.0 41.0 31.0 41.0 48-49 36.628554682929085 39.0 36.0 41.0 30.5 41.0 50-51 36.428977094915815 39.0 36.0 41.0 30.0 41.0 52-53 36.19440645979432 39.0 35.0 40.0 30.0 41.0 54-55 35.98551424186267 39.0 35.0 40.0 29.0 41.0 56-57 35.73969754240448 38.5 35.0 40.0 29.0 41.0 58-59 35.473685481654435 38.0 35.0 40.0 28.0 41.0 60-61 35.13871971546209 38.0 34.0 40.0 28.0 41.0 62-63 34.7578566996759 37.5 34.0 40.0 27.0 41.0 64-65 34.415341059071004 37.0 34.0 39.5 26.0 41.0 66-67 34.03051992935535 36.0 33.0 39.0 26.0 40.5 68-69 33.58614986679294 36.0 33.0 39.0 26.0 40.0 70-71 33.196860721740094 35.5 32.5 38.5 25.5 40.0 72-73 32.76659067468903 35.0 32.0 37.5 24.5 40.0 74-75 32.324674533911335 35.0 32.0 37.0 24.0 39.0 76-77 31.026066540943145 34.0 30.5 35.5 22.5 38.5 78-79 31.653070089503174 34.5 31.0 36.0 23.0 38.5 80-81 31.48854507678115 34.5 31.0 36.0 22.5 37.5 82-83 31.151085249800666 34.0 31.0 35.0 21.5 37.0 84-85 30.81534663771216 34.0 31.0 35.0 20.0 36.5 86-87 30.482750093204125 34.0 31.0 35.0 19.5 36.0 88-89 30.102609239318262 34.0 30.0 35.0 18.0 36.0 90-91 29.64896672680714 34.0 30.0 35.0 12.5 35.0 92-93 29.220573348245992 34.0 29.0 35.0 6.0 35.0 94-95 28.809781603002126 34.0 29.0 35.0 2.0 35.0 96-97 28.362099392336308 34.0 29.0 35.0 2.0 35.0 98-99 27.675059800311857 33.0 28.0 35.0 2.0 35.0 100-101 25.973660309737042 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1778861578776585 1101 2 0.2253128765426169 1101 3 0.23003378323977586 1101 4 0.1896119437387469 1101 5 0.07325122190530209 1101 6 0.22708574728478936 1101 7 0.21263497170749446 1101 8 0.14666931693006546 1101 9 0.17848237203118344 1101 10-11 0.25351976505377394 1101 12-13 0.2934517871211568 1101 14-15 0.36042470106804103 1101 16-17 0.31746679709176107 1101 18-19 0.3716393252905519 1101 20-21 0.2190063156511144 1101 22-23 0.2583611720261487 1101 24-25 0.3799852406883346 1101 26-27 0.33574098059575164 1101 28-29 0.4549039927997356 1101 30-31 0.4110717666046142 1101 32-33 0.31788941066044885 1101 34-35 0.40022614133046375 1101 36-37 0.4501530427193998 1101 38-39 0.34064278374270174 1101 40-41 0.3643500612765038 1101 42-43 0.23061504860729798 1101 44-45 0.2640110283666104 1101 46-47 0.2151573096320334 1101 48-49 0.18760046706647415 1101 50-51 0.2062633580194131 1101 52-53 0.15081801129325356 1101 54-55 0.2750209256294909 1101 56-57 0.3413845813907841 1101 58-59 0.3269443365098468 1101 60-61 0.15931673888522369 1101 62-63 0.10397666135044403 1101 64-65 0.26185598859095194 1101 66-67 0.35496662423067704 1101 68-69 0.20523666151530762 1101 70-71 0.025887372919697782 1101 72-73 0.09689510611195828 1101 74-75 0.12215888659606833 1101 76-77 0.11066188231297858 1101 78-79 -0.008185844205453208 1101 80-81 -0.06163440075038551 1101 82-83 -0.08860249473110926 1101 84-85 0.008075854290428452 1101 86-87 0.08457546271550243 1101 88-89 0.29641810492876175 1101 90-91 0.3312978919702374 1101 92-93 0.30202947326405294 1101 94-95 0.046316649814468036 1101 96-97 0.2579556048706877 1101 98-99 0.22648163449071035 1101 100-101 0.25401450649106394 1102 1 0.2826337879181864 1102 2 0.274526584956881 1102 3 0.175921114378653 1102 4 0.1449632086006929 1102 5 0.09659225027734664 1102 6 0.18546795776899216 1102 7 0.2134528306232184 1102 8 0.2636033821756385 1102 9 0.2837871293001015 1102 10-11 0.3169226268370622 1102 12-13 0.21251580373858303 1102 14-15 0.2794116066503989 1102 16-17 0.24262740491949586 1102 18-19 0.2690298396693507 1102 20-21 0.2750518208006838 1102 22-23 0.2991656810939247 1102 24-25 0.22711936087552687 1102 26-27 0.24511816440436007 1102 28-29 0.20256367288533283 1102 30-31 0.36326943110414334 1102 32-33 0.45180645846447476 1102 34-35 0.3283261254263081 1102 36-37 0.3639804971395435 1102 38-39 0.24555162404092812 1102 40-41 0.28377915045059154 1102 42-43 0.30770776980753567 1102 44-45 0.28339047731187605 1102 46-47 0.4508855028722891 1102 48-49 0.2300643092201895 1102 50-51 0.15918514333264966 1102 52-53 0.2481157514579806 1102 54-55 0.26772911337558014 1102 56-57 0.14232581796262167 1102 58-59 0.06123971032968001 1102 60-61 0.22857208725842781 1102 62-63 0.18844818569102273 1102 64-65 0.29170446540703665 1102 66-67 0.07064763515634809 1102 68-69 -0.019077170758549755 1102 70-71 -0.007389412321252564 1102 72-73 -0.011792656848719218 1102 74-75 -0.04507422488239854 1102 76-77 0.11769802329122925 1102 78-79 0.24163414972205288 1102 80-81 0.27910408199361214 1102 82-83 0.23069815637519397 1102 84-85 0.17422941127789215 1102 86-87 0.3483267699885211 1102 88-89 0.270201316892539 1102 90-91 0.1676215269844299 1102 92-93 0.20239430484873466 1102 94-95 0.10971806576585053 1102 96-97 0.3852360432469233 1102 98-99 0.10662988045563182 1102 100-101 0.4024238504207389 1103 1 0.06393737134041544 1103 2 0.11087738105343448 1103 3 0.14013133918209775 1103 4 0.10386842767540827 1103 5 0.03438313171229623 1103 6 0.11937218913085701 1103 7 0.07512265797101492 1103 8 0.10007764237871442 1103 9 0.06382347713306302 1103 10-11 0.13095160633213965 1103 12-13 0.1729969186688507 1103 14-15 0.1426178213396625 1103 16-17 0.09839861111442616 1103 18-19 0.046861118559952786 1103 20-21 -0.016110124598554876 1103 22-23 0.08010134147873771 1103 24-25 0.0638385738659153 1103 26-27 0.07386406539602319 1103 28-29 0.07113909463871693 1103 30-31 0.024940246164781854 1103 32-33 0.15887502549066568 1103 34-35 0.23165619930723125 1103 36-37 0.14703776473071173 1103 38-39 0.31559528094899036 1103 40-41 0.3402017532715007 1103 42-43 0.23631489714347254 1103 44-45 -0.0053950309651611406 1103 46-47 4.5782320732001835E-4 1103 48-49 0.0907587701265129 1103 50-51 0.049222577649750576 1103 52-53 0.11343073308652407 1103 54-55 0.027813661595722294 1103 56-57 0.16959311281465972 1103 58-59 -0.08614136904292735 1103 60-61 -0.04332550667307089 1103 62-63 0.14363565359035846 1103 64-65 -0.06926062882076423 1103 66-67 0.03272538791206614 1103 68-69 0.21268866227847383 1103 70-71 0.07592762375002593 1103 72-73 -0.04213914651723627 1103 74-75 -0.0726896885148065 1103 76-77 -0.11584272974105048 1103 78-79 0.3015394280595345 1103 80-81 0.21138352571373176 1103 82-83 0.18529102113754803 1103 84-85 0.287736384530632 1103 86-87 0.39197231935440513 1103 88-89 0.359753784199242 1103 90-91 0.5852324471377237 1103 92-93 0.5845409011902412 1103 94-95 0.5109635716723737 1103 96-97 0.5532840263372769 1103 98-99 0.5513545061124603 1103 100-101 0.7161441696495352 1104 1 0.15671120949727424 1104 2 0.20993328352147245 1104 3 0.12581931744141173 1104 4 0.13413732562291614 1104 5 0.0309339245923681 1104 6 0.0920542429029183 1104 7 0.12832683070708129 1104 8 0.1001039901619265 1104 9 0.11153366657362085 1104 10-11 0.20489171970289277 1104 12-13 0.17364032212492475 1104 14-15 0.23081067393886912 1104 16-17 0.15119721618750503 1104 18-19 0.2857308268899885 1104 20-21 0.1991860809442727 1104 22-23 0.18509184686685387 1104 24-25 0.018287020664097042 1104 26-27 0.1692973829206892 1104 28-29 0.21474133532948514 1104 30-31 0.13931220911227626 1104 32-33 0.03266467419943808 1104 34-35 0.04322132349938812 1104 36-37 0.16892265691182473 1104 38-39 0.05675324720125019 1104 40-41 -0.022921277702202758 1104 42-43 0.07103478256825468 1104 44-45 -0.005971859219016551 1104 46-47 -0.08196415912460253 1104 48-49 -0.13206466773224435 1104 50-51 0.02426064078656509 1104 52-53 0.140625174080661 1104 54-55 0.07976584413316345 1104 56-57 -0.03350195186914817 1104 58-59 -0.07471231233120079 1104 60-61 -0.13873082967806738 1104 62-63 -0.01585500612048918 1104 64-65 0.013175233146959897 1104 66-67 0.11121825707273558 1104 68-69 0.12719151724898126 1104 70-71 0.2407374774445472 1104 72-73 0.3403732651706335 1104 74-75 0.14928897354476334 1104 76-77 0.2148595892402696 1104 78-79 0.13469891307329007 1104 80-81 0.36218459242255463 1104 82-83 0.2740764636109283 1104 84-85 0.2710916429835635 1104 86-87 0.28979679230464583 1104 88-89 0.3602009907696271 1104 90-91 0.5450398448424565 1104 92-93 0.3589570625440679 1104 94-95 0.2021746098879511 1104 96-97 0.2553049483591323 1104 98-99 0.308883855660973 1104 100-101 0.5339939458815728 1105 1 0.07691300267933343 1105 2 0.099949488693035 1105 3 0.06812863911630274 1105 4 0.05604436305534222 1105 5 -0.05574858158156104 1105 6 0.017456049998052947 1105 7 0.02756803269022612 1105 8 0.005110201995712771 1105 9 0.047316772347819835 1105 10-11 0.0757406495105073 1105 12-13 -0.05945974414057531 1105 14-15 0.026533427266194565 1105 16-17 0.06183711087619059 1105 18-19 0.04966754199379153 1105 20-21 0.1587418255750208 1105 22-23 0.13663396916462034 1105 24-25 0.11094791518601 1105 26-27 0.13146026942116862 1105 28-29 0.11503359921068324 1105 30-31 -0.015543723612630345 1105 32-33 -0.06293736531549854 1105 34-35 -0.0608202034790537 1105 36-37 0.11422997991915196 1105 38-39 0.19431638003459994 1105 40-41 0.20167731654153442 1105 42-43 -0.06698587657286481 1105 44-45 0.12857588093101668 1105 46-47 -0.0015643899390553884 1105 48-49 0.06293986739695612 1105 50-51 0.08915490583253671 1105 52-53 0.13569918542695802 1105 54-55 0.14457449022096114 1105 56-57 0.16751842990222343 1105 58-59 0.1637097302695807 1105 60-61 0.11322725805817413 1105 62-63 0.17999011096789275 1105 64-65 0.33277831792667456 1105 66-67 0.2718173489766613 1105 68-69 0.3024686863294761 1105 70-71 0.26782941169275176 1105 72-73 0.43858601401188224 1105 74-75 0.33624265101279605 1105 76-77 0.135932733694613 1105 78-79 0.14233906729444 1105 80-81 0.20313868193182572 1105 82-83 0.17595423898066898 1105 84-85 0.09073084826125921 1105 86-87 0.25967051325997303 1105 88-89 0.39753910627852207 1105 90-91 0.26010708693987183 1105 92-93 0.2688671893939052 1105 94-95 0.46722080399147714 1105 96-97 0.29869079465348136 1105 98-99 0.44796070491898377 1105 100-101 0.6966679597250867 1106 1 -0.1599018334570239 1106 2 -0.004437113460745934 1106 3 -0.13793840494161458 1106 4 -0.08440503407813083 1106 5 -0.07868400929925912 1106 6 -0.018641925521777125 1106 7 -0.011332963787147321 1106 8 -0.006102338724616629 1106 9 -0.10483312416656787 1106 10-11 -0.07670371269371401 1106 12-13 -0.21724743643421363 1106 14-15 -0.03679999744517204 1106 16-17 -0.03326342779728009 1106 18-19 -0.051463489527058925 1106 20-21 -0.29493910977045346 1106 22-23 -0.11005768939224225 1106 24-25 -0.027137303961346504 1106 26-27 -2.814871386362938E-4 1106 28-29 -0.04744110049828265 1106 30-31 0.026706505974665617 1106 32-33 0.030971832339744765 1106 34-35 -0.011119923405402687 1106 36-37 -0.0573660743776756 1106 38-39 0.0054802619559168875 1106 40-41 -0.015870005853848568 1106 42-43 -0.07711764368681884 1106 44-45 -0.013332594837812906 1106 46-47 -0.01685239063352384 1106 48-49 -0.25448066798690405 1106 50-51 -0.03227050000012355 1106 52-53 0.1372181166695654 1106 54-55 0.0778319797924425 1106 56-57 0.19264921470240637 1106 58-59 0.01975781338703797 1106 60-61 0.028925317645786208 1106 62-63 -0.05087852855037056 1106 64-65 0.06871226988923951 1106 66-67 0.15613893566471404 1106 68-69 0.13232143064005442 1106 70-71 0.02217215239389958 1106 72-73 -0.05023868181351787 1106 74-75 0.006296929560434705 1106 76-77 0.01734598624977224 1106 78-79 0.019235047118172588 1106 80-81 0.13062679136615074 1106 82-83 0.07710857189823273 1106 84-85 0.028963414476059768 1106 86-87 0.1367190943876686 1106 88-89 0.19049774401490538 1106 90-91 0.33086885985633785 1106 92-93 0.29041549867197247 1106 94-95 0.193227218728925 1106 96-97 0.2199528622640834 1106 98-99 0.12722703074561537 1106 100-101 0.1262669015675435 1107 1 -0.11036024650536191 1107 2 -0.0768060665081336 1107 3 -0.062428887703973146 1107 4 -0.031248997459293548 1107 5 -0.16581027109274515 1107 6 -0.058697671439652765 1107 7 -0.055436387372552076 1107 8 -0.14020921819552967 1107 9 -0.12157587301855699 1107 10-11 -0.08413002666430458 1107 12-13 -0.18113749861756645 1107 14-15 -0.04418203994126202 1107 16-17 -0.06611015136935094 1107 18-19 -0.026996271876285505 1107 20-21 -0.07331678610532322 1107 22-23 -0.09079349936823178 1107 24-25 -0.10928731649740797 1107 26-27 -0.09913539804907145 1107 28-29 -0.2175519824718819 1107 30-31 -0.2015644444841982 1107 32-33 -0.18386227386209697 1107 34-35 -0.11581428082528333 1107 36-37 -0.1384321134133657 1107 38-39 -0.06990773460174893 1107 40-41 -0.15646604446038026 1107 42-43 -0.09224132147180342 1107 44-45 -0.13730808289694352 1107 46-47 -0.06642785881565771 1107 48-49 -0.12463040043812867 1107 50-51 -0.14034371959792935 1107 52-53 -0.02221927420463743 1107 54-55 -0.16342609805759167 1107 56-57 -0.18730213185063604 1107 58-59 -0.0822310148419021 1107 60-61 -0.18035452550670072 1107 62-63 -0.17189415231511163 1107 64-65 -0.05483744404023838 1107 66-67 -0.17192917916104022 1107 68-69 -0.34166930397278605 1107 70-71 -0.5512880021496827 1107 72-73 -0.3022757343271607 1107 74-75 -0.13808815998766022 1107 76-77 -0.14236200271849953 1107 78-79 -0.09510574836794561 1107 80-81 -0.04112958903423802 1107 82-83 -0.23495766364859705 1107 84-85 -0.16323848151759535 1107 86-87 -0.1472282044876998 1107 88-89 0.15292752516474195 1107 90-91 0.28602456669786136 1107 92-93 0.061750170690370254 1107 94-95 0.010599480843161757 1107 96-97 0.16187727098014193 1107 98-99 0.23596070846283368 1107 100-101 0.5812805331159403 1108 1 0.009817300282115582 1108 2 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-0.6132898355415577 2316 46-47 -0.666889319116656 2316 48-49 -0.6478509038886457 2316 50-51 -0.5895614783069121 2316 52-53 -0.6496052822539369 2316 54-55 -0.8241676003890177 2316 56-57 -0.5461715616509295 2316 58-59 -0.47065918045303334 2316 60-61 -0.6212375564008781 2316 62-63 -0.3018362072129577 2316 64-65 -0.3706397583267105 2316 66-67 -0.326459585641075 2316 68-69 -0.4537833529590358 2316 70-71 -0.2154438483939103 2316 72-73 -0.23655570701040318 2316 74-75 -0.47601549318129344 2316 76-77 -0.3879364712954292 2316 78-79 -0.2583754079184857 2316 80-81 -0.430752234810317 2316 82-83 -0.184639998256813 2316 84-85 -0.22798212054639322 2316 86-87 -0.47266048118096293 2316 88-89 -0.6019859491443853 2316 90-91 -0.570414696473204 2316 92-93 -0.4939929070282325 2316 94-95 -0.4584568496755814 2316 96-97 -0.24696258576264185 2316 98-99 -0.5379358044289617 2316 100-101 -1.089453748062784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 4.0 5 28.0 6 32.0 7 121.0 8 313.0 9 672.0 10 1169.0 11 1793.0 12 2671.0 13 3686.0 14 4947.0 15 6574.0 16 8325.0 17 11147.0 18 10074.0 19 6999.0 20 6239.0 21 6300.0 22 6571.0 23 7507.0 24 8759.0 25 10677.0 26 13146.0 27 16794.0 28 21090.0 29 26663.0 30 33588.0 31 42817.0 32 54651.0 33 71859.0 34 98341.0 35 141605.0 36 217948.0 37 331091.0 38 269384.0 39 26300.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.07074980141565 19.817458950725896 15.552268694325763 22.55952255353269 2 15.797215033485454 20.479191668503535 39.70557020515793 24.01802309285308 3 19.578241122477024 24.136671265644004 29.7698062170554 26.515281394823564 4 13.21097958964304 17.128140794113307 37.08628735055864 32.574592265685006 5 13.765680782574558 37.442820469030345 34.44080578997396 14.350692958421135 6 32.43238278730682 36.63459628326435 17.025672634452054 13.907348294976774 7 28.818988061707934 32.41714357245294 20.487083404767155 18.27678496107197 8 26.640120498648876 35.41600335262727 19.38863535552272 18.555240793201133 9 25.999801345949226 16.83368574017683 20.044193723076255 37.12231919079769 10-11 24.89325746381367 26.94123105643136 27.278364668968873 20.8871468107861 12-13 25.754873912703296 23.87467130217068 27.970731010365252 22.39972377476077 14-15 22.49971426472149 25.508098554179494 25.983235503016548 26.00895167808247 16-17 22.078152680605108 28.743914518889824 26.93357743289983 22.24435536760524 18-19 22.064954432026298 27.231252364119268 29.20860852361942 21.49518468023501 20-21 22.500938844486534 26.357820846701664 29.60282115964982 21.53841914916198 22-23 22.221069439332513 26.25284633434317 29.523332968222427 22.002751258101892 24-25 22.21570999918021 26.452502592889036 29.573834152388063 21.757953255542695 26-27 21.925307776224717 26.78203378080271 29.27982523487805 22.01283320809452 28-29 21.872815475524312 26.954604071894096 29.412939290332083 21.759641162249512 30-31 22.050453967328504 26.659793063965665 29.330395214013173 21.95935775469266 32-33 21.855007034530427 26.642263513237708 29.28827424055645 22.214455211675414 34-35 22.297352458547977 26.825644333053038 29.147311503158058 21.72969170524093 36-37 22.498286433485557 26.408211512785844 29.173933379255214 21.919568674473386 38-39 22.080608917556248 26.733891788960644 29.1348586660063 22.050640627476806 40-41 22.310947652223074 26.666918386097464 29.24040940464613 21.78172455703333 42-43 22.182668552723523 26.65606610180216 29.02299261158319 22.13827273389112 44-45 22.173601870069366 26.826460719563073 28.977877286222387 22.022060124145177 46-47 22.55305151671007 27.032904458286733 28.462703382289313 21.951340642713888 48-49 22.405913091514947 27.195145762508922 28.325535702987498 22.07340544298863 50-51 22.529104179082545 27.225605690758233 28.27837011154561 21.96692001861361 52-53 22.68081284994115 27.216152229078368 28.14414684092007 21.95888808006041 54-55 22.52577826322555 27.35793764369683 28.14469639425767 21.971587698819945 56-57 22.486482000038098 27.478515428344398 27.988416836066865 22.04658573555064 58-59 22.392631567489314 27.182473270145017 28.187377031781928 22.237518130583744 60-61 22.256363052523586 27.408544301213965 28.325312335872294 22.009780310390152 62-63 22.40470728461683 27.321190944640833 28.368784917531354 21.905316853210984 64-65 22.246056172834468 27.27907900716515 28.37303693060442 22.101827889395956 66-67 22.107542594966162 27.471542126904563 28.121011611737462 22.299903666391817 68-69 22.5148854473662 27.12406761857334 28.24806176236077 22.11298517169969 70-71 22.338443912886117 27.212373426074844 28.12152185330623 22.327660807732812 72-73 22.307727370446266 27.35660170951335 27.99158033379323 22.34409058624715 74-75 22.31847645949498 27.587128850282877 27.826023952780204 22.268370737441934 76-77 22.374126806595314 27.30401281182563 28.044883569677225 22.27697681190183 78-79 22.368412101025108 27.425382272983324 27.964197369602665 22.242008256388903 80-81 22.45600357033034 27.39888372751195 27.77180228207242 22.373310420085286 82-83 22.375982940202608 27.493820109484496 27.927832621317876 22.20236432899502 84-85 22.275385632204824 27.56979001511336 27.85491310072886 22.29991125195295 86-87 22.480801310572478 27.503007023645274 27.7148253068933 22.301366358888952 88-89 22.42736201027014 27.522294154944717 27.53977843270118 22.510565402083962 90-91 22.269292054303914 27.536736240663707 27.7614603871814 22.432511317850974 92-93 22.411386118405836 27.2516089967752 27.86772209598193 22.469282788837035 94-95 22.191936550440442 27.08610564585697 28.010969513406426 22.710988290296157 96-97 22.398176192536596 26.96875008504026 28.02032394216718 22.612749780255964 98-99 22.11279477886865 27.164489721220814 27.76777313414152 22.954942365769014 100-101 21.868478868045457 27.181455780304148 28.09821594416976 22.851849407480636 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 126.0 1 102.5 2 94.0 3 136.0 4 141.0 5 125.0 6 230.5 7 552.5 8 932.0 9 1156.0 10 1131.5 11 1018.0 12 1040.0 13 1159.5 14 1285.5 15 1406.5 16 1538.0 17 1662.5 18 1818.5 19 2051.0 20 2300.0 21 2662.5 22 3187.0 23 3855.5 24 4749.5 25 5999.0 26 7832.0 27 10139.5 28 12263.5 29 14518.0 30 17323.0 31 20734.5 32 25216.5 33 28439.5 34 30596.5 35 33401.5 36 37356.5 37 40934.0 38 43288.5 39 45178.5 40 46588.0 41 48352.0 42 49162.0 43 50270.0 44 52562.5 45 54898.0 46 55482.5 47 55886.5 48 56736.0 49 55876.0 50 53998.0 51 52045.5 52 50505.0 53 49506.5 54 48972.5 55 45237.5 56 40023.0 57 36055.5 58 32372.0 59 28389.0 60 23365.5 61 19593.0 62 16352.0 63 11621.0 64 7560.5 65 5370.0 66 4051.5 67 3257.5 68 2486.5 69 1831.0 70 1328.5 71 801.5 72 520.0 73 403.0 74 268.0 75 186.0 76 150.0 77 86.0 78 40.5 79 26.0 80 21.0 81 14.5 82 7.0 83 4.0 84 3.5 85 2.0 86 1.0 87 0.5 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050547931412648 2 0.0 3 0.0 4 6.803220916910902E-5 5 0.012177765441270515 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005238480106021395 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012926119742130715 24-25 4.4220935959920864E-4 26-27 0.002279079007165152 28-29 0.00241514342550337 30-31 0.001190563660459408 32-33 0.0 34-35 0.0 36-37 3.0614494126099065E-4 38-39 3.401610458455451E-4 40-41 1.0204831375366354E-4 42-43 0.010817121257888335 44-45 0.0 46-47 0.0 48-49 6.803220916910902E-5 50-51 0.0 52-53 1.3606441833821805E-4 54-55 3.401610458455451E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.381127320918816E-4 84-85 3.401610458455451E-5 86-87 0.0 88-89 0.0 90-91 0.0061228988252198125 92-93 0.0021770306934114888 94-95 0.0 96-97 0.0 98-99 0.0012926119742130715 100-101 0.010272863584535462 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1469892.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.59733205725687 #Duplication Level Percentage of deduplicated Percentage of total 1 80.59881356182026 51.258695095120224 2 11.780426428672284 14.98407382720712 3 3.388865909291308 6.465684915921513 4 1.4631092290205518 3.721993738962285 5 0.7548808327999288 2.400420349361784 6 0.4486800312842441 1.7120911762226687 7 0.30031927212347376 1.3369653130701176 8 0.20038938056801617 1.0195383981385715 9 0.14741285463158316 0.843755783896164 >10 0.8391097501203904 9.533711844887668 >50 0.05170362023928571 2.2246296315357674 >100 0.023821274525293815 2.716454766489032 >500 0.0017167686283468357 0.7140496555768566 >1k 7.510862749017407E-4 1.0679355036102642 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4637 0.3154653539171585 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3889 0.264577261458665 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2649 0.18021732208896982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 6.803220916910902E-5 6 0.0 0.0 0.0 0.0 6.803220916910902E-5 7 0.0 0.0 0.0 0.0 6.803220916910902E-5 8 0.0 0.0 0.0 0.0 6.803220916910902E-5 9 0.0 0.0 0.0 0.0 6.803220916910902E-5 10-11 0.0 0.0 0.0 0.0 6.803220916910902E-5 12-13 0.0 0.0 0.0 0.0 2.381127320918816E-4 14-15 3.401610458455451E-5 0.0 0.0 0.0 4.081932550146541E-4 16-17 6.803220916910902E-5 0.0 0.0 3.401610458455451E-5 4.7622546418376314E-4 18-19 6.803220916910902E-5 0.0 0.0 6.803220916910902E-5 5.782737779374267E-4 20-21 6.803220916910902E-5 0.0 0.0 6.803220916910902E-5 7.143381962756447E-4 22-23 6.803220916910902E-5 0.0 0.0 6.803220916910902E-5 7.483543008601993E-4 24-25 6.803220916910902E-5 0.0 0.0 2.0409662750732707E-4 7.483543008601993E-4 26-27 6.803220916910902E-5 0.0 0.0 2.0409662750732707E-4 8.163865100293083E-4 28-29 6.803220916910902E-5 0.0 0.0 5.782737779374266E-4 8.844187191984173E-4 30-31 6.803220916910902E-5 0.0 0.0 0.0022110467979960433 8.844187191984173E-4 32-33 6.803220916910902E-5 0.0 0.0 0.004660206328083968 8.844187191984173E-4 34-35 1.7008052292277255E-4 0.0 0.0 0.008197881204877638 9.524509283675263E-4 36-37 2.0409662750732707E-4 0.0 0.0 0.011633507767917644 9.524509283675263E-4 38-39 2.0409662750732707E-4 0.0 0.0 0.015103150435542202 0.0010204831375366353 40-41 2.0409662750732707E-4 0.0 0.0 0.01741624554729191 0.0010204831375366353 42-43 2.0409662750732707E-4 0.0 0.0 0.019491227926949732 0.001122531451290299 44-45 2.0409662750732707E-4 0.0 0.0 0.02245062902580598 0.001190563660459408 46-47 2.0409662750732707E-4 0.0 0.0 0.02758706081807371 0.0012245797650439624 48-49 2.0409662750732707E-4 0.0 0.0 0.033233734179109756 0.0012245797650439624 50-51 2.0409662750732707E-4 0.0 0.0 0.03809803713470106 0.0012926119742130715 52-53 2.0409662750732707E-4 0.0 0.0 0.04820082019631375 0.0016327730200586166 54-55 2.0409662750732707E-4 0.0 0.0 0.06582116237111299 0.0017008052292277257 56-57 2.0409662750732707E-4 0.0 0.0 0.09959915422357561 0.0018028535429813891 58-59 2.0409662750732707E-4 0.0 0.0 0.13531606403735785 0.0018368696475659437 60-61 2.0409662750732707E-4 0.0 0.0 0.17167927983824663 0.002143014588826934 62-63 2.0409662750732707E-4 0.0 0.0 0.22300958165633938 0.0023811273209188156 64-65 2.0409662750732707E-4 0.0 0.0 0.2849869242093977 0.0023811273209188156 66-67 2.0409662750732707E-4 0.0 0.0 0.3802660331507349 0.0023811273209188156 68-69 3.401610458455451E-4 0.0 0.0 0.4939818707769006 0.0023811273209188156 70-71 3.401610458455451E-4 0.0 0.0 0.5941592987784137 0.00241514342550337 72-73 3.401610458455451E-4 0.0 0.0 0.6950170488716179 0.002585223948426143 74-75 3.401610458455451E-4 0.0 0.0 0.8165565905522311 0.0027553044713489153 76-77 4.0819325501465414E-4 0.0 0.0 0.9651389353775652 0.002857352785102579 78-79 4.0819325501465414E-4 0.0 0.0 1.0931755530338283 0.0029594010988562426 80-81 4.7622546418376314E-4 0.0 0.0 1.2297161968362302 0.003061449412609906 82-83 4.7622546418376314E-4 0.0 0.0 1.382346458107126 0.0033335782492863422 84-85 4.7622546418376314E-4 0.0 0.0 1.5846742481760565 0.0033335782492863422 86-87 4.7622546418376314E-4 0.0 0.0 1.8296922494986028 0.003537674876793669 88-89 4.7622546418376314E-4 0.0 0.0 2.12042789538279 0.0037757876088855506 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2490 0.0 51.535904 1 GTATCAA 4555 0.0 40.171474 1 ATCAACG 5730 0.0 31.08749 3 TCAACGC 5775 0.0 30.84525 4 CAACGCA 5920 0.0 30.250227 5 TATCAAC 5965 0.0 29.942387 2 AACGCAG 6200 0.0 28.88114 6 TAGCCCG 70 0.008390867 27.143818 5 ACGCAGA 7035 0.0 25.252346 7 CGCAGAG 7035 0.0 25.117308 8 TGGACCG 160 1.8595711E-6 23.750841 5 GCAGAGT 8075 0.0 21.882385 9 TACATGG 4805 0.0 21.650091 2 TGGTATC 1295 0.0 21.641693 2 GTACATG 4915 0.0 21.563877 1 GGTAAGG 810 0.0 21.11186 3 GTAAGGT 860 0.0 20.989115 4 GAGTACT 5025 0.0 20.796051 12-13 ACATGGG 4795 0.0 20.704592 3 GTGGTAT 1465 0.0 20.11405 1 >>END_MODULE