FastQCFastQC Report
Thu 26 May 2016
SRR937265_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937265_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513554
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38860.25674670345425404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29360.19398052530666235No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22240.14693892652657256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19830.13101613817544666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.050.9622461
GTATCAA43650.043.9011961
TCAACGC52900.035.7646034
ATCAACG53550.035.4180833
CAACGCA54400.035.0394335
TATCAAC54600.034.4769252
AACGCAG57050.033.3874976
ACGCAGA65500.028.7901767
CGCAGAG66250.028.1066888
GTACATG50850.025.9026581
TACATGG49850.025.3654422
ACATGGG50800.024.1416873
GCAGAGT75750.023.5776399
GAGTACT42350.022.26321212-13
AGAGTAC65800.021.0794510-11
GTACTTT44700.020.880614-15
CAGAGTA70050.020.20740110-11
GTACTAG3553.6379788E-1220.0917821
TAGACCG1200.004764081419.7917379
AGTACTT46600.019.62119912-13
Produced by FastQC (version 0.11.5)