##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937265_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1513554 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.362371610130857 31.0 31.0 34.0 30.0 34.0 2 31.45511491496174 33.0 31.0 34.0 28.0 34.0 3 31.50565754508924 33.0 31.0 34.0 28.0 34.0 4 35.1464427433709 37.0 35.0 37.0 33.0 37.0 5 35.12504542289208 37.0 35.0 37.0 32.0 37.0 6 35.18255906297364 37.0 35.0 37.0 32.0 37.0 7 35.10498799514256 37.0 35.0 37.0 32.0 37.0 8 35.081435482315136 37.0 35.0 37.0 32.0 37.0 9 36.65667429110557 39.0 37.0 39.0 32.0 39.0 10-11 36.552106499008296 39.0 37.0 39.0 32.0 39.0 12-13 36.431168626953514 39.0 37.0 39.0 32.0 39.0 14-15 37.53398821581523 40.0 37.0 41.0 32.0 41.0 16-17 37.4592204837092 40.0 37.0 41.0 32.0 41.0 18-19 37.38999698722345 40.0 37.0 41.0 32.0 41.0 20-21 37.29706472316151 40.0 37.0 41.0 31.5 41.0 22-23 37.15531523817452 39.5 37.0 41.0 31.0 41.0 24-25 37.00039906075369 39.0 36.0 41.0 31.0 41.0 26-27 36.83101131509018 39.0 36.0 41.0 30.5 41.0 28-29 36.63296288074294 39.0 36.0 40.5 30.0 41.0 30-31 36.637286149024085 39.0 36.0 41.0 30.0 41.0 32-33 36.49425293051982 39.0 36.0 40.5 30.0 41.0 34-35 36.29346062314262 39.0 35.0 40.0 30.0 41.0 36-37 36.29749880083565 39.0 35.0 41.0 30.0 41.0 38-39 36.26638527597959 39.0 35.0 41.0 30.0 41.0 40-41 36.099860328736206 39.0 35.0 41.0 29.5 41.0 42-43 35.878490955724075 39.0 35.0 40.5 28.0 41.0 44-45 35.67171075495159 39.0 35.0 40.0 27.0 41.0 46-47 35.403131635871596 38.5 35.0 40.0 26.5 41.0 48-49 35.127325817248675 38.0 34.5 40.0 25.5 41.0 50-51 34.219762889199856 37.5 33.5 39.5 24.0 40.5 52-53 34.360512079516155 38.0 33.5 39.5 24.0 40.5 54-55 34.70090264371142 38.0 34.0 40.0 24.5 41.0 56-57 34.46239612197516 38.0 34.0 40.0 23.5 41.0 58-59 34.19833947120486 38.0 34.0 40.0 23.0 41.0 60-61 33.90046143051387 37.5 33.0 40.0 22.5 41.0 62-63 33.569893773198714 37.0 33.0 40.0 22.0 41.0 64-65 33.19324054510113 36.5 32.5 39.5 20.5 41.0 66-67 32.79634555489926 36.0 32.0 39.0 19.5 41.0 68-69 32.27859561006743 35.5 31.0 39.0 18.0 40.0 70-71 31.846952272598138 35.0 31.0 38.5 15.0 40.0 72-73 31.414639649460806 35.0 31.0 37.5 12.0 40.0 74-75 30.939716719720607 35.0 30.0 37.0 8.0 39.0 76-77 30.494684035059205 34.5 30.0 36.5 7.0 39.0 78-79 30.00568496399864 34.0 29.5 36.0 4.0 38.5 80-81 29.547134426654086 34.0 29.0 35.5 2.0 37.0 82-83 29.08559687992632 34.0 29.0 35.0 2.0 37.0 84-85 28.611423180144214 34.0 28.5 35.0 2.0 36.5 86-87 28.148410297881675 34.0 27.0 35.0 2.0 36.0 88-89 27.728142504330865 33.0 26.5 35.0 2.0 36.0 90-91 27.26498228672383 33.0 26.0 35.0 2.0 35.0 92-93 26.776833201854707 33.0 25.0 35.0 2.0 35.0 94-95 26.19261321366796 32.5 24.5 35.0 2.0 35.0 96-97 25.517476746782737 32.0 21.5 34.0 2.0 35.0 98-99 24.735757693481702 32.0 17.0 34.0 2.0 35.0 100-101 22.849787982457183 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16294284084789012 1101 2 0.14684572334069657 1101 3 0.2677566452574709 1101 4 0.20864268907941153 1101 5 0.2719459354916225 1101 6 0.17917991847840398 1101 7 0.29415610864905517 1101 8 0.28390272142484463 1101 9 0.122804516378757 1101 10-11 0.16631005693251666 1101 12-13 0.36452497564534525 1101 14-15 0.5933004857996096 1101 16-17 0.38377382611550104 1101 18-19 0.49391270785732644 1101 20-21 0.560244165492854 1101 22-23 0.28046365436542686 1101 24-25 0.4345216667527936 1101 26-27 0.39467441578044316 1101 28-29 0.5447551323353679 1101 30-31 0.4771937710803229 1101 32-33 0.36086538857563255 1101 34-35 0.29354589173051693 1101 36-37 0.4052698940520827 1101 38-39 0.5429276510798999 1101 40-41 0.35316116147356524 1101 42-43 0.36136037575474944 1101 44-45 0.21385752593019447 1101 46-47 0.42263820295763566 1101 48-49 0.39955109564468927 1101 50-51 0.49219085758868175 1101 52-53 0.42440897400527433 1101 54-55 0.5054992911393938 1101 56-57 0.5278018448310888 1101 58-59 0.6223837338732068 1101 60-61 0.5360165026154036 1101 62-63 0.5735329275071379 1101 64-65 0.4621147977966231 1101 66-67 0.5018219564386541 1101 68-69 0.33059581909760993 1101 70-71 0.4175005380281753 1101 72-73 0.503373428092992 1101 74-75 0.5053852744561915 1101 76-77 0.6393216897256409 1101 78-79 0.8139869546292182 1101 80-81 0.5943327157613574 1101 82-83 0.5627095980682739 1101 84-85 0.6967608165418255 1101 86-87 0.8197416274725526 1101 88-89 0.8799532441789815 1101 90-91 0.7719905886963687 1101 92-93 0.8115945939475218 1101 94-95 0.9457891236226743 1101 96-97 0.9345550105801763 1101 98-99 1.1832118306637582 1101 100-101 1.550323367765369 1102 1 0.2203782560123564 1102 2 0.22355274635154387 1102 3 0.20998227995733387 1102 4 0.020225073741961808 1102 5 0.16850051185789994 1102 6 0.362841892588726 1102 7 0.24002972789926247 1102 8 0.30497157415505427 1102 9 0.36967754403961095 1102 10-11 0.3591407670735052 1102 12-13 0.5052362584592274 1102 14-15 0.663299083247324 1102 16-17 0.6792041579270105 1102 18-19 0.5804425417627712 1102 20-21 0.6700287208747895 1102 22-23 0.7849855368783025 1102 24-25 0.6868754371909489 1102 26-27 0.8699121753650019 1102 28-29 0.6634861282014413 1102 30-31 0.5495778312534156 1102 32-33 0.5289379041250797 1102 34-35 0.6031590839934609 1102 36-37 0.6412338071010311 1102 38-39 0.8535298225185457 1102 40-41 0.9139123540925453 1102 42-43 0.8445562709517205 1102 44-45 0.7466916345632626 1102 46-47 0.9957487132888758 1102 48-49 1.0504360521925449 1102 50-51 0.965872098811495 1102 52-53 0.8152128612328795 1102 54-55 1.0587486271747366 1102 56-57 1.0129467033081738 1102 58-59 0.930829481172843 1102 60-61 1.0592175045412162 1102 62-63 1.0406244206678963 1102 64-65 0.9318610257401403 1102 66-67 0.9250479525654569 1102 68-69 1.0022311321274984 1102 70-71 0.7245417101303353 1102 72-73 0.6516411861131672 1102 74-75 0.6586628863052404 1102 76-77 0.8047104754210466 1102 78-79 0.9915442300095698 1102 80-81 0.955387210312086 1102 82-83 1.140346036736279 1102 84-85 1.035334465821041 1102 86-87 1.132640478278752 1102 88-89 1.1261872564315354 1102 90-91 1.0845318336233554 1102 92-93 0.8582546314568056 1102 94-95 0.8075983890988496 1102 96-97 1.0570746957611306 1102 98-99 1.290772531508349 1102 100-101 1.2551746702509448 1103 1 0.14258496901891604 1103 2 0.129540643296032 1103 3 0.25208730687941383 1103 4 0.21682139741244555 1103 5 0.05562798998315799 1103 6 0.10454920493943831 1103 7 0.037501129238066255 1103 8 0.2261468167530225 1103 9 0.24980563295024183 1103 10-11 0.2643621019651903 1103 12-13 0.24957945295476236 1103 14-15 0.11116296259696412 1103 16-17 0.153901449525101 1103 18-19 0.18629015169093321 1103 20-21 0.21354871925541374 1103 22-23 0.25418421990533346 1103 24-25 0.20111822173861071 1103 26-27 0.12111262386100918 1103 28-29 -0.07064555325374045 1103 30-31 -0.16314841896441123 1103 32-33 -0.11232711688275998 1103 34-35 -0.08571859347313193 1103 36-37 0.04114135320476464 1103 38-39 -0.07809411850810477 1103 40-41 0.06833764377554985 1103 42-43 -0.04752525745507086 1103 44-45 0.023003811018625697 1103 46-47 0.033806406984403736 1103 48-49 0.06367376915776646 1103 50-51 -0.0021124942593502283 1103 52-53 0.04347585450367575 1103 54-55 0.03363315881500739 1103 56-57 0.14780749880772248 1103 58-59 0.11317201911810315 1103 60-61 0.273589310899375 1103 62-63 0.13390868581563353 1103 64-65 0.115095759186012 1103 66-67 0.26907369708114715 1103 68-69 0.20577183912477892 1103 70-71 0.21132863580285388 1103 72-73 0.43719523181821884 1103 74-75 0.577699279960818 1103 76-77 0.4546633463763534 1103 78-79 0.6010391703479812 1103 80-81 0.35629674366853337 1103 82-83 0.3885039995646231 1103 84-85 0.3230009576423285 1103 86-87 0.40983098875099344 1103 88-89 0.3288034244661624 1103 90-91 0.49155868177718176 1103 92-93 0.6491138850313085 1103 94-95 0.42363726990064876 1103 96-97 0.6178674230146548 1103 98-99 0.5586723275025101 1103 100-101 0.9282392550841045 1104 1 0.1458441163011699 1104 2 0.1055573065533082 1104 3 0.2307026200175919 1104 4 -0.018542777624567464 1104 5 0.10942038923563047 1104 6 0.12634388559501275 1104 7 0.13414091371828363 1104 8 0.32810438542914966 1104 9 0.29150278188981105 1104 10-11 0.2456584104608197 1104 12-13 0.2915375070358053 1104 14-15 0.4173810862295966 1104 16-17 0.3502347354736699 1104 18-19 0.35713707925118143 1104 20-21 0.4477188848825122 1104 22-23 0.5692098423504532 1104 24-25 0.540941967747024 1104 26-27 0.49246211886654123 1104 28-29 0.3587128628006795 1104 30-31 0.34969227460986474 1104 32-33 0.37460812316729886 1104 34-35 0.49951114173848055 1104 36-37 0.4135912438152971 1104 38-39 0.4575584390557239 1104 40-41 0.39943840343424597 1104 42-43 0.5398450267494965 1104 44-45 0.44405010131157496 1104 46-47 0.37800486478365514 1104 48-49 0.47062901482465946 1104 50-51 0.23841647574609226 1104 52-53 0.2250866360402597 1104 54-55 0.21291009618737178 1104 56-57 0.45048346675713447 1104 58-59 0.3474028603594945 1104 60-61 0.42791643567270654 1104 62-63 0.40933956935659666 1104 64-65 0.38608258737694 1104 66-67 0.3698376897445925 1104 68-69 0.3837360162420964 1104 70-71 0.4781084769367645 1104 72-73 0.4837776345032019 1104 74-75 0.43733881480422454 1104 76-77 0.3388782385991256 1104 78-79 0.4590161933596413 1104 80-81 0.7091328085602377 1104 82-83 0.6841649997067769 1104 84-85 0.7472790911910892 1104 86-87 0.6903655582488426 1104 88-89 0.6731193329187981 1104 90-91 0.9753184202132736 1104 92-93 1.0015135188973154 1104 94-95 0.7500326356705891 1104 96-97 1.0033226981525623 1104 98-99 1.027752901251116 1104 100-101 1.2226291011067296 1105 1 0.233524597241896 1105 2 0.3342685829188383 1105 3 0.21970219818710035 1105 4 0.2691069429689463 1105 5 0.26735948194929904 1105 6 0.20226393027846967 1105 7 0.19039682181859519 1105 8 0.24509866778215184 1105 9 0.14902211278929656 1105 10-11 0.4741711386787557 1105 12-13 0.39944156713181656 1105 14-15 0.4815121379006939 1105 16-17 0.3706982363429532 1105 18-19 0.6009494825067421 1105 20-21 0.5070823075917374 1105 22-23 0.5226310765239432 1105 24-25 0.5500634900064512 1105 26-27 0.5661655297573134 1105 28-29 0.5972083130575072 1105 30-31 0.513854266513242 1105 32-33 0.5282331221554486 1105 34-35 0.5229306040440136 1105 36-37 0.39434916258100117 1105 38-39 0.43582607386384353 1105 40-41 0.6167697004685664 1105 42-43 0.5130763292703264 1105 44-45 0.452984992809391 1105 46-47 0.6382607872747741 1105 48-49 0.6247365555169111 1105 50-51 0.6771961893870468 1105 52-53 0.712008991347453 1105 54-55 0.8300314673433391 1105 56-57 1.0951603077234324 1105 58-59 1.0839724160703526 1105 60-61 1.2477253347670754 1105 62-63 1.2960328859965955 1105 64-65 1.296044038006137 1105 66-67 1.178615811402672 1105 68-69 1.2987011949893983 1105 70-71 1.2087458727046076 1105 72-73 1.1772804555508287 1105 74-75 1.170908786949024 1105 76-77 1.3266541485702454 1105 78-79 1.3827233368656557 1105 80-81 1.2678405501841148 1105 82-83 1.3960579213191586 1105 84-85 1.5161537571669967 1105 86-87 1.5089584342603573 1105 88-89 1.6030237419918407 1105 90-91 1.606374138714724 1105 92-93 1.8484780932307707 1105 94-95 1.794039269832023 1105 96-97 1.8195602040100702 1105 98-99 1.6933706130950235 1105 100-101 1.8058488819887266 1106 1 0.035988662950313 1106 2 0.09646200066923782 1106 3 -0.022494927069573123 1106 4 0.11485899635703589 1106 5 0.021799933694680362 1106 6 0.05882764234782201 1106 7 0.13304312572003596 1106 8 0.07341118204179509 1106 9 0.05684352506399648 1106 10-11 -0.079099309644306 1106 12-13 -0.08101916182096147 1106 14-15 -0.010811898147679244 1106 16-17 -0.013374459299292596 1106 18-19 0.043905220585685356 1106 20-21 -0.056466488894685085 1106 22-23 -0.03744121984840376 1106 24-25 -0.034772096349932724 1106 26-27 0.09475396804160141 1106 28-29 0.17209209699545625 1106 30-31 0.2691075177110491 1106 32-33 0.13191210915065454 1106 34-35 0.1498492385409378 1106 36-37 -0.05434750636720764 1106 38-39 -0.09395090537412187 1106 40-41 -0.08757521169205518 1106 42-43 0.01991739132827064 1106 44-45 0.04021833755044213 1106 46-47 0.16214853539249674 1106 48-49 0.4147933088821887 1106 50-51 0.10720845267702828 1106 52-53 0.17666077095046262 1106 54-55 0.2928551611603538 1106 56-57 0.12487525752951001 1106 58-59 0.11236763623919188 1106 60-61 0.1379032950682344 1106 62-63 0.28626503292792194 1106 64-65 0.35229617695205917 1106 66-67 0.30592872493221535 1106 68-69 0.40039310121800753 1106 70-71 0.31052333677031996 1106 72-73 0.5647091573532492 1106 74-75 0.34896961857119635 1106 76-77 0.4140493402198011 1106 78-79 0.480446785416877 1106 80-81 0.5319618711971188 1106 82-83 0.26145548006887154 1106 84-85 0.36870694562239237 1106 86-87 0.25091956248303404 1106 88-89 0.5209666463952196 1106 90-91 0.7733345539554257 1106 92-93 1.0112932312640837 1106 94-95 1.0477339424306997 1106 96-97 1.2672444385526234 1106 98-99 1.3769028683996076 1106 100-101 1.6308246406015492 1107 1 -0.017018607373547212 1107 2 -0.15548822698736942 1107 3 -0.03920565630508932 1107 4 -0.054750223735496206 1107 5 0.17629156180660033 1107 6 0.06681903178749593 1107 7 -0.09240163083427433 1107 8 -0.0594379220193062 1107 9 -0.04380410290104919 1107 10-11 0.02410004594062798 1107 12-13 0.002434655099065708 1107 14-15 0.09627070080950517 1107 16-17 0.014668382369208643 1107 18-19 0.17058327008263774 1107 20-21 0.14649419444231881 1107 22-23 0.09639539063848446 1107 24-25 0.20187442087119223 1107 26-27 0.254227649836416 1107 28-29 -0.010329462452297378 1107 30-31 0.038092977063328703 1107 32-33 0.14920115960229907 1107 34-35 0.2405472171705867 1107 36-37 0.18097323241519803 1107 38-39 0.3377144761342876 1107 40-41 0.35487586049711695 1107 42-43 0.17984500937924253 1107 44-45 0.2649626828541045 1107 46-47 0.12621551049715407 1107 48-49 0.26067116376945876 1107 50-51 0.3278329477934321 1107 52-53 0.20560023999651378 1107 54-55 0.3193702709495909 1107 56-57 0.3998984362766578 1107 58-59 0.3454921313555843 1107 60-61 0.32551069757980855 1107 62-63 0.2886655249599386 1107 64-65 0.4036146116293011 1107 66-67 0.3578500365280135 1107 68-69 0.4302062483869875 1107 70-71 0.5528557613131646 1107 72-73 0.3896412275596113 1107 74-75 0.494463532428874 1107 76-77 0.4829928024361472 1107 78-79 0.40694101138441496 1107 80-81 0.47972156496226503 1107 82-83 0.646932273782614 1107 84-85 0.7865662850510446 1107 86-87 0.683303026168133 1107 88-89 0.7003999834004304 1107 90-91 0.687433658016527 1107 92-93 0.8538884247721974 1107 94-95 0.6079486556568163 1107 96-97 0.6244229653940678 1107 98-99 0.7806020587499027 1107 100-101 0.9521780040443026 1108 1 -0.013495873132161051 1108 2 -0.03215845954234098 1108 3 -0.09891497962668083 1108 4 -0.06535249545618171 1108 5 0.11808808813171368 1108 6 -0.01766526581199912 1108 7 0.14225198967798747 1108 8 0.04703450594180225 1108 9 0.1056361434859312 1108 10-11 0.06963666670499435 1108 12-13 0.17058218748164222 1108 14-15 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-1.9575575182269702 2316 86-87 -2.193100399425834 2316 88-89 -1.9459416833446141 2316 90-91 -2.242004313372444 2316 92-93 -2.022774075046108 2316 94-95 -2.2074308196794057 2316 96-97 -2.184969159262529 2316 98-99 -2.173731834058348 2316 100-101 -2.4095312958653174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7076.0 3 1604.0 4 1498.0 5 1651.0 6 1996.0 7 2293.0 8 2812.0 9 3475.0 10 4263.0 11 5237.0 12 6344.0 13 7588.0 14 8609.0 15 9948.0 16 11362.0 17 13361.0 18 12338.0 19 10625.0 20 10683.0 21 11473.0 22 12552.0 23 14051.0 24 15833.0 25 17912.0 26 20860.0 27 24399.0 28 28329.0 29 34201.0 30 41522.0 31 50905.0 32 63160.0 33 80901.0 34 106949.0 35 146118.0 36 207752.0 37 275148.0 38 211603.0 39 27116.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78820439303048 19.165375904941612 14.942083074021125 23.10433662800678 2 15.080102297723478 19.77029390718999 40.769816871173774 24.379786923912754 3 19.282130313476653 23.988487370627027 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26.4481585201957 28.972372837593596 22.295345157467544 24-25 22.212994781512666 26.63616425624143 29.08146546616523 22.069375496080674 26-27 22.00969545775352 26.856464322648733 28.835909475515724 22.297930744082024 28-29 21.92589453891811 27.20318587937752 28.80546075085324 22.065458830851124 30-31 22.108271955124383 26.863527174589553 28.77181343143253 22.25638743885353 32-33 21.886360814759023 26.797162744887064 28.762582663609805 22.553893776744108 34-35 22.337005006827493 26.98880291306327 28.630148549675177 22.044043530434063 36-37 22.378979334653227 26.681763457930963 28.64468837793132 22.29456882948449 38-39 22.04627796302291 26.97266205528756 28.6176917816698 22.363368200019725 40-41 22.379768052583046 26.849379816377724 28.727936761593565 22.04291536944567 42-43 22.16578446351545 26.849978684135966 28.514039342994764 22.470197509353827 44-45 22.170858796189012 27.11729576196561 28.475671524196056 22.236173917649317 46-47 22.53187317198471 27.19835182367903 27.962427458659878 22.307347545676386 48-49 22.29584498171845 27.36111731806588 27.90511138587231 22.43792631434336 50-51 22.437002665692027 27.39703714886162 27.903260407521145 22.26269977792521 52-53 22.61990924158518 27.36231122070929 27.79739670485901 22.220382832846518 54-55 22.433816328517068 27.433091272912918 27.847200656324304 22.28589174224571 56-57 22.37176166874902 27.626633128407953 27.67627857716144 22.32532662568159 58-59 22.347043154780124 27.256646891156816 27.858417189238953 22.537892764824104 60-61 22.218791256398724 27.501960118497397 27.98761277921139 22.291635845892486 62-63 22.26693567551073 27.412391749424614 28.155905981938545 22.16476659312611 64-65 22.178812549642572 27.37043288324067 28.018433942282233 22.432320624834524 66-67 22.060465876810824 27.553653552199798 27.821466187289438 22.564414383699937 68-69 22.41455600164019 27.275426024432758 27.980814131855354 22.3292038420717 70-71 22.307081271616994 27.30074304532742 27.807039899382723 22.58513578367286 72-73 22.25346657883674 27.369272986669934 27.79714596870562 22.580114465787705 74-75 22.209277001321954 27.675705446503102 27.66014911570534 22.4548684364696 76-77 22.32877238325751 27.469242054307703 27.730015941698934 22.47196962073586 78-79 22.30886534835094 27.562157469008568 27.679069417959223 22.44990776468127 80-81 22.348290531814687 27.55195656677917 27.529715707540642 22.570037193865495 82-83 22.39343093454668 27.56851962004178 27.632205686132956 22.40584375927859 84-85 22.333663288023306 27.699083970324594 27.509252465641854 22.458000276010242 86-87 22.49006444540939 27.575466527573315 27.521453119539085 22.413015907478208 88-89 22.40672058626391 27.60034622405595 27.31803790921714 22.674895280463 90-91 22.326378212458582 27.622237013387846 27.50371277748192 22.547671996671657 92-93 22.46220881448433 27.357677116030004 27.71727635353663 22.462837715949043 94-95 22.25389118967928 27.262358068889547 27.761301529466593 22.722449211964584 96-97 22.44231544483816 27.05602072718583 27.757496162306467 22.74416766566954 98-99 22.201700337452603 27.253923569219157 27.60190191532075 22.94247417800749 100-101 22.339535845778347 26.972407231208372 27.975145823853058 22.71291109916022 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2001.0 1 1116.5 2 226.5 3 202.0 4 176.0 5 176.0 6 267.0 7 512.5 8 839.0 9 1024.0 10 956.5 11 910.0 12 970.0 13 1055.0 14 1139.5 15 1241.5 16 1399.0 17 1485.0 18 1617.0 19 1842.5 20 2170.0 21 2546.0 22 3066.0 23 3874.5 24 4817.0 25 6092.0 26 7996.5 27 10365.0 28 12540.5 29 14788.0 30 17473.5 31 21044.5 32 25669.5 33 29037.5 34 31518.0 35 34331.5 36 38149.0 37 41904.0 38 44211.5 39 46220.0 40 47933.5 41 49461.5 42 50091.0 43 51311.5 44 53686.0 45 56352.5 46 57169.0 47 57138.5 48 57732.5 49 57092.0 50 55224.0 51 53313.0 52 51839.5 53 50615.5 54 49813.5 55 46566.5 56 41963.5 57 37844.5 58 34515.5 59 30340.0 60 24845.5 61 20801.5 62 17072.5 63 12440.0 64 8514.0 65 6177.5 66 4534.5 67 3460.0 68 2711.0 69 2115.5 70 1578.5 71 1033.5 72 694.0 73 485.5 74 329.5 75 248.5 76 185.0 77 103.5 78 57.0 79 41.5 80 34.5 81 24.5 82 15.0 83 16.0 84 14.5 85 7.5 86 5.5 87 6.0 88 5.5 89 4.0 90 2.5 91 2.0 92 2.0 93 3.0 94 4.0 95 3.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31416123904399845 2 0.27973894555463497 3 0.2811264084400028 4 0.2809942691175868 5 0.2692338694225644 6 0.20646769127497266 7 0.21472639892597156 8 0.2121496821388599 9 0.20970510467416426 10-11 0.21776560334153922 12-13 0.21859147410663907 14-15 0.20214012846584925 16-17 0.21343804053241575 18-19 0.20081873524168942 20-21 0.20557575084866478 22-23 0.20375883516544502 24-25 0.1957644061592781 26-27 0.2085158507724204 28-29 0.20739266653188454 30-31 0.21446212028113965 32-33 0.21746828986610323 34-35 0.20590609915470479 36-37 0.20339545202880108 38-39 0.1944430129351183 40-41 0.19510370954719816 42-43 0.19437694327391028 44-45 0.21036580128624416 46-47 0.20058749142746146 48-49 0.20821853729698445 50-51 0.21485853824838758 52-53 0.22404222115629835 54-55 0.21968162351657092 56-57 0.18773694232250715 58-59 0.17696758754560457 60-61 0.1852262951966035 62-63 0.1865146535901593 64-65 0.18195584696680792 66-67 0.19708579938343793 68-69 0.1819888817974119 70-71 0.19021455461780684 72-73 0.20210709363524523 74-75 0.1925930624212945 76-77 0.20151246668437336 78-79 0.19923306337269764 80-81 0.18638251426774333 82-83 0.1994973420175296 84-85 0.18106390654050003 86-87 0.18618430528411936 88-89 0.19632599827954603 90-91 0.1911064950441147 92-93 0.19711883421404194 94-95 0.19569833649807009 96-97 0.19034669394022283 98-99 0.19632599827954603 100-101 0.181129976201708 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1513554.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.29058846043854 #Duplication Level Percentage of deduplicated Percentage of total 1 81.87346212306086 53.45566521308069 2 10.989090196738939 14.349683311798433 3 3.2183509847603924 6.303840890017136 4 1.3391161951630477 3.4972673759639537 5 0.7007159947558623 2.2875079820625914 6 0.4246685229863884 1.6636114659843941 7 0.2741182805669408 1.2528140692185414 8 0.18894322375241734 0.9868971411526111 9 0.14541253083036584 0.8544662736692623 >10 0.7742462816446686 9.037585323758677 >50 0.04786484949673629 2.119323809128972 >100 0.02178207773539691 2.573798421803969 >500 0.001519594437250367 0.6787643577536846 >1k 7.091440707168379E-4 0.9387743646070985 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3886 0.25674670345425404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2936 0.19398052530666235 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2224 0.14693892652657256 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1983 0.13101613817544666 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.321393224159825E-4 2 0.0 0.0 0.0 0.0 1.321393224159825E-4 3 0.0 0.0 0.0 0.0 1.321393224159825E-4 4 0.0 0.0 0.0 0.0 1.321393224159825E-4 5 0.0 0.0 0.0 0.0 1.321393224159825E-4 6 0.0 0.0 0.0 0.0 1.321393224159825E-4 7 0.0 0.0 0.0 0.0 1.321393224159825E-4 8 0.0 0.0 0.0 0.0 1.9820898362397379E-4 9 0.0 0.0 0.0 0.0 1.9820898362397379E-4 10-11 0.0 0.0 0.0 0.0 1.9820898362397379E-4 12-13 0.0 0.0 0.0 0.0 2.312438142279694E-4 14-15 0.0 0.0 0.0 0.0 5.615921202679257E-4 16-17 0.0 0.0 0.0 0.0 8.919404263078819E-4 18-19 0.0 0.0 0.0 9.910449181198689E-5 0.00105711457932786 20-21 0.0 0.0 0.0 1.9820898362397379E-4 0.0011892539017438427 22-23 0.0 0.0 0.0 2.312438142279694E-4 0.0012222887323478384 24-25 6.606966120799125E-5 0.0 0.0 3.303483060399563E-4 0.0012553235629518338 26-27 6.606966120799125E-5 0.0 0.0 4.624876284559388E-4 0.0014535325465758076 28-29 6.606966120799125E-5 0.0 0.0 9.91044918119869E-4 0.001519602207783799 30-31 6.606966120799125E-5 0.0 0.0 0.0027749257707356326 0.0015526370383877946 32-33 6.606966120799125E-5 0.0 0.0 0.005417712219055283 0.0016517415301997814 34-35 6.606966120799125E-5 0.0 0.0 0.009183682907910785 0.0018499505138237552 36-37 6.606966120799125E-5 0.0 0.0 0.012949653596766288 0.0018829853444277509 38-39 1.9820898362397379E-4 0.0 0.0 0.016120997334749865 0.001982089836239738 40-41 1.9820898362397379E-4 0.0 0.0 0.018995027597297487 0.001982089836239738 42-43 1.9820898362397379E-4 0.0 0.0 0.02183602302924111 0.0020151246668437335 44-45 1.9820898362397379E-4 0.0 0.0 0.025998411685344558 0.002048159497447729 46-47 2.312438142279694E-4 0.0 0.0 0.03135005424319185 0.0020811943280517244 48-49 2.64278644831965E-4 0.0 0.0 0.037329358582515064 0.0021802988198637114 50-51 2.64278644831965E-4 0.0 0.0 0.042813140462778336 0.0021802988198637114 52-53 2.64278644831965E-4 0.0 0.0 0.05434229634357281 0.0023785078034876854 54-55 2.64278644831965E-4 0.0 0.0 0.07436140368959415 0.0024445774646956763 56-57 2.64278644831965E-4 0.0 0.0 0.10960956794405749 0.002477612295299672 58-59 2.64278644831965E-4 0.0 0.0 0.14690589169596857 0.0025106471259036677 60-61 2.64278644831965E-4 0.0 0.0 0.1850941558741875 0.0025106471259036677 62-63 2.64278644831965E-4 0.0 0.0 0.23854451179145245 0.0027088561095276417 64-65 2.64278644831965E-4 0.0 0.0 0.30428382469340376 0.0027749257707356326 66-67 2.64278644831965E-4 0.0 0.0 0.39605458411130356 0.0029070650931516152 68-69 2.64278644831965E-4 0.0 0.0 0.5090997744381767 0.0030061695849636022 70-71 2.973134754359607E-4 0.0 0.0 0.6112104358351271 0.0030722392461715936 72-73 3.303483060399563E-4 0.0 0.0 0.7154353263907334 0.003237413399191572 74-75 3.303483060399563E-4 0.0 0.0 0.8375320603031011 0.003303483060399563 76-77 3.303483060399563E-4 0.0 0.0 0.982885314960682 0.003303483060399563 78-79 3.303483060399563E-4 0.0 0.0 1.1087149847313014 0.003369552721607554 80-81 5.2855728966393E-4 0.0 0.0 1.2455452530930513 0.003369552721607554 82-83 5.2855728966393E-4 0.0 0.0 1.4034517433801503 0.003633831366439519 84-85 5.2855728966393E-4 0.0 0.0 1.6155353558578023 0.003633831366439519 86-87 5.2855728966393E-4 0.0 0.0 1.8639572819998493 0.0036668661970435147 88-89 5.2855728966393E-4 0.0 0.0 2.1499728453692435 0.0037659706888555017 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2230 0.0 50.962246 1 GTATCAA 4365 0.0 43.901196 1 TCAACGC 5290 0.0 35.764603 4 ATCAACG 5355 0.0 35.418083 3 CAACGCA 5440 0.0 35.039433 5 TATCAAC 5460 0.0 34.476925 2 AACGCAG 5705 0.0 33.387497 6 ACGCAGA 6550 0.0 28.790176 7 CGCAGAG 6625 0.0 28.106688 8 GTACATG 5085 0.0 25.902658 1 TACATGG 4985 0.0 25.365442 2 ACATGGG 5080 0.0 24.141687 3 GCAGAGT 7575 0.0 23.577639 9 GAGTACT 4235 0.0 22.263212 12-13 AGAGTAC 6580 0.0 21.07945 10-11 GTACTTT 4470 0.0 20.8806 14-15 CAGAGTA 7005 0.0 20.207401 10-11 GTACTAG 355 3.6379788E-12 20.091782 1 TAGACCG 120 0.0047640814 19.791737 9 AGTACTT 4660 0.0 19.621199 12-13 >>END_MODULE