FastQCFastQC Report
Thu 26 May 2016
SRR937265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513554
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47990.31706830413715004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37790.24967724970499897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27240.17997375713056818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27700.045.8213841
GTATCAA48350.039.426171
TCAACGC60950.030.944314
ATCAACG61500.030.6675743
CAACGCA61850.030.4172155
TATCAAC62700.030.2321722
AACGCAG64200.029.2970396
ACGCAGA74600.024.768697
CGCAGAG76100.024.0932248
TAAGGTG7900.024.0545335
GTAAGGT6850.023.5804624
GCGTTAA1252.2443222E-422.818021
GCGTGCC3301.8189894E-1221.5908419
TATAGGG4200.021.4915772
GGTAAGG7100.021.411923
GTACATG50750.021.0757581
GCAGAGT85900.021.0127419
AGGTAAG7300.020.8252932
GAGTACT51150.020.66220312-13
TACATGG49250.020.257092
Produced by FastQC (version 0.11.5)