##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937265_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1513554 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04428781530094 31.0 31.0 34.0 30.0 34.0 2 32.27136131251346 33.0 31.0 34.0 31.0 34.0 3 32.36265901315711 34.0 31.0 34.0 30.0 34.0 4 35.99176904160671 37.0 35.0 37.0 35.0 37.0 5 35.870089867953176 37.0 35.0 37.0 35.0 37.0 6 35.950643320291185 37.0 35.0 37.0 35.0 37.0 7 35.90474142316693 37.0 35.0 37.0 35.0 37.0 8 35.87542102891605 37.0 35.0 37.0 35.0 37.0 9 37.558940744763646 39.0 37.0 39.0 35.0 39.0 10-11 37.48534079391948 39.0 37.0 39.0 35.0 39.0 12-13 37.40601029101043 39.0 37.0 39.0 34.0 39.0 14-15 38.6083813329422 40.0 38.0 41.0 34.0 41.0 16-17 38.57843823213443 40.0 38.0 41.0 34.0 41.0 18-19 38.56543737455023 40.0 38.0 41.0 34.0 41.0 20-21 38.44172259463488 40.0 38.0 41.0 34.0 41.0 22-23 38.32156963015525 40.0 38.0 41.0 34.0 41.0 24-25 38.17986342079635 40.0 38.0 41.0 33.5 41.0 26-27 38.126404806171436 40.0 38.0 41.0 33.0 41.0 28-29 38.04344840025529 40.0 37.0 41.0 33.0 41.0 30-31 38.13348879524616 40.0 38.0 41.0 34.0 41.0 32-33 38.08347637414985 40.0 37.0 41.0 33.0 41.0 34-35 37.979846771241725 40.0 37.0 41.0 33.0 41.0 36-37 37.8287590003396 40.0 37.0 41.0 33.0 41.0 38-39 37.70100934621428 40.0 37.0 41.0 33.0 41.0 40-41 37.54303282208629 40.0 37.0 41.0 33.0 41.0 42-43 37.364657950756964 40.0 37.0 41.0 32.0 41.0 44-45 37.1126312638994 40.0 36.0 41.0 31.5 41.0 46-47 36.82546575807669 39.0 36.0 41.0 31.0 41.0 48-49 36.56219963080273 39.0 36.0 41.0 30.0 41.0 50-51 36.36094417510046 39.0 35.5 41.0 30.0 41.0 52-53 36.12937166430798 39.0 35.0 40.0 29.5 41.0 54-55 35.890403976336486 39.0 35.0 40.0 29.0 41.0 56-57 35.63216707167369 38.0 35.0 40.0 28.0 41.0 58-59 35.36853888265632 38.0 34.5 40.0 28.0 41.0 60-61 35.036994055051885 38.0 34.0 40.0 27.5 41.0 62-63 34.6568890175045 37.0 34.0 40.0 26.5 41.0 64-65 34.29469183127922 37.0 33.5 39.5 26.0 41.0 66-67 33.86050778498818 36.0 33.0 39.0 26.0 40.5 68-69 33.46046292368822 36.0 33.0 39.0 25.5 40.0 70-71 33.045349554756555 35.0 32.0 38.5 25.0 40.0 72-73 32.589384653603375 35.0 32.0 37.5 24.0 39.5 74-75 32.14652896427877 35.0 31.0 37.0 23.0 39.0 76-77 30.823990092193604 33.5 29.5 35.5 21.5 38.0 78-79 31.480089577246666 34.0 31.0 36.0 22.0 38.5 80-81 31.348167954364364 34.0 31.0 36.0 22.0 37.5 82-83 31.015027544441757 34.0 31.0 35.0 20.5 37.0 84-85 30.63790951627758 34.0 31.0 35.0 19.5 36.5 86-87 30.25543819381403 34.0 30.0 35.0 18.0 36.0 88-89 29.83101197578679 34.0 30.0 35.0 15.5 36.0 90-91 29.369143750404675 34.0 29.0 35.0 8.5 35.0 92-93 28.983050158765394 34.0 29.0 35.0 4.5 35.0 94-95 28.525792274342376 33.5 29.0 35.0 2.0 35.0 96-97 27.99668726718703 33.0 28.5 35.0 2.0 35.0 98-99 27.420618293103516 33.0 27.0 35.0 2.0 35.0 100-101 25.372938131047853 31.5 21.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1900536849958101 1101 2 0.2530007868540878 1101 3 0.17823541858258096 1101 4 0.07251730940217271 1101 5 0.05079588981286065 1101 6 0.08903938056609206 1101 7 0.08652141947406733 1101 8 0.1119705384162657 1101 9 0.200590682734493 1101 10-11 0.12423828632731215 1101 12-13 0.1253786516790214 1101 14-15 0.1853516158207853 1101 16-17 0.16827495941226545 1101 18-19 0.11181116551251336 1101 20-21 0.17121857115837003 1101 22-23 0.15629086258731206 1101 24-25 0.21340940433046285 1101 26-27 0.0994944241942548 1101 28-29 0.06041317605657781 1101 30-31 0.04098815797227928 1101 32-33 0.024394226267091312 1101 34-35 0.0828127770151923 1101 36-37 0.029490970644431513 1101 38-39 -0.1469757785089172 1101 40-41 -0.08038948681538471 1101 42-43 0.012675345879372912 1101 44-45 0.08071316173177223 1101 46-47 -0.004383018154619833 1101 48-49 0.011023796551057785 1101 50-51 -0.0839715980551432 1101 52-53 -0.16252589752898672 1101 54-55 -0.07486853973915686 1101 56-57 -0.12851988734244912 1101 58-59 -0.1513469175935782 1101 60-61 -0.345183944785191 1101 62-63 -0.2567211032565453 1101 64-65 -0.2579343848187108 1101 66-67 -0.03515133364147971 1101 68-69 -0.17754114236193175 1101 70-71 -0.36808057019250384 1101 72-73 -0.235815733015869 1101 74-75 -0.13604770710292513 1101 76-77 -0.0758832594719685 1101 78-79 -0.20671202325343074 1101 80-81 -0.2534249752958182 1101 82-83 -0.06883472796274503 1101 84-85 -0.10159696742534763 1101 86-87 -0.11378566400986756 1101 88-89 -0.1113427083243046 1101 90-91 0.1913309643721739 1101 92-93 0.15008214855226854 1101 94-95 0.17804601554720279 1101 96-97 0.09653518550224405 1101 98-99 0.17949665830747108 1101 100-101 0.7285531524846398 1102 1 0.3030499502508164 1102 2 0.20899480982357943 1102 3 0.25815033041059365 1102 4 0.21521127069532753 1102 5 0.22628035474815533 1102 6 0.23205700777128868 1102 7 0.08454031437075571 1102 8 0.1700092308779091 1102 9 0.24090866651163623 1102 10-11 0.298188484932318 1102 12-13 0.2051488129185941 1102 14-15 0.21034267005459384 1102 16-17 0.20874803310559287 1102 18-19 0.33639395116116333 1102 20-21 0.3424699804536999 1102 22-23 0.34684220049631875 1102 24-25 0.3144976860061206 1102 26-27 0.34672505773431084 1102 28-29 0.18670199573690383 1102 30-31 0.37600822525882904 1102 32-33 0.290493458639439 1102 34-35 0.19921989658637074 1102 36-37 0.1559364536169099 1102 38-39 0.054504903131494586 1102 40-41 0.2528396290911772 1102 42-43 0.32045712852332287 1102 44-45 0.27846988723970156 1102 46-47 0.19807967485365907 1102 48-49 0.25307015629852714 1102 50-51 0.15829233711097146 1102 52-53 0.32646024597369205 1102 54-55 0.27421728387371047 1102 56-57 0.31331667395897256 1102 58-59 0.09525031973804232 1102 60-61 0.16044132381256304 1102 62-63 -0.06670156642247349 1102 64-65 -0.016261814246057327 1102 66-67 0.1892181471804193 1102 68-69 0.24778418683327175 1102 70-71 0.08951267705768373 1102 72-73 0.20040522471157374 1102 74-75 0.007111523938796438 1102 76-77 0.11122283461771687 1102 78-79 0.1010567228468453 1102 80-81 0.2582283461457884 1102 82-83 0.3985442434629505 1102 84-85 0.16463375422376814 1102 86-87 0.26198191869126575 1102 88-89 0.42904922412302327 1102 90-91 0.5827594818564243 1102 92-93 0.4024040019352064 1102 94-95 0.4358256853720093 1102 96-97 0.35212224354093635 1102 98-99 0.521093886648373 1102 100-101 1.1961029475861373 1103 1 0.11833885877558004 1103 2 0.0993121893958886 1103 3 0.1515141560744908 1103 4 0.10262917990833387 1103 5 0.08789522200354583 1103 6 0.051892042913451064 1103 7 8.12112103986351E-4 1103 8 0.12186499751916813 1103 9 0.0741602977662481 1103 10-11 0.06867729805487244 1103 12-13 0.11917439021638643 1103 14-15 0.05297566549042898 1103 16-17 0.13847779137892502 1103 18-19 0.13253619022643903 1103 20-21 0.16011864156638467 1103 22-23 0.10305280527946081 1103 24-25 0.1044165589222743 1103 26-27 0.07491918363354699 1103 28-29 -0.15008277370481693 1103 30-31 -0.02166608179899754 1103 32-33 0.021458781167559948 1103 34-35 0.15593576345276006 1103 36-37 0.0554512576815398 1103 38-39 0.028425511593418662 1103 40-41 0.06709662716025377 1103 42-43 0.22242365502032158 1103 44-45 0.1044376388278252 1103 46-47 0.031115242980874314 1103 48-49 -1.9081275559784672E-4 1103 50-51 0.25456914258023033 1103 52-53 0.05287372127208556 1103 54-55 0.10450475784492141 1103 56-57 -0.011698595675483148 1103 58-59 0.1157056892808015 1103 60-61 -0.08004177749859309 1103 62-63 -0.09487602221621927 1103 64-65 -0.06272550771980434 1103 66-67 -0.001529730472405788 1103 68-69 0.0922700005947803 1103 70-71 -0.11481175382939313 1103 72-73 -0.020747707498294687 1103 74-75 -0.02480927286329404 1103 76-77 0.07280210206250004 1103 78-79 0.14557694829177592 1103 80-81 0.23876880319631155 1103 82-83 0.13810170569370683 1103 84-85 0.19884521621866114 1103 86-87 0.15608326329919464 1103 88-89 0.1399774418794486 1103 90-91 0.08003118982004409 1103 92-93 0.26953573971642086 1103 94-95 0.4177195072867086 1103 96-97 0.24099428840461812 1103 98-99 0.37728627348146304 1103 100-101 0.8676880940602132 1104 1 0.08679013972447791 1104 2 0.08008495401958271 1104 3 0.08244537073094449 1104 4 0.05622880406623665 1104 5 0.020351128167838795 1104 6 0.08209983088858053 1104 7 0.060290683210602936 1104 8 0.03049879071587469 1104 9 0.24704679276137398 1104 10-11 0.004047800691658665 1104 12-13 0.13846966896016255 1104 14-15 0.07694339993233967 1104 16-17 0.08929673881304012 1104 18-19 -0.009227615209404405 1104 20-21 -0.06665603347236271 1104 22-23 -0.052746136537827226 1104 24-25 0.0691120193461714 1104 26-27 0.006776568202532474 1104 28-29 -0.035703173193063265 1104 30-31 0.04486082870273833 1104 32-33 -0.019690553654349685 1104 34-35 -0.044025490833547565 1104 36-37 -0.011131561064800621 1104 38-39 -0.055309436520253996 1104 40-41 -0.06114301724598192 1104 42-43 0.03483329169473848 1104 44-45 0.03356548754792499 1104 46-47 -0.04885608345740167 1104 48-49 -0.0388910197461243 1104 50-51 -0.04389983831215716 1104 52-53 0.042954991923473074 1104 54-55 -0.01636539980766827 1104 56-57 0.014729968323415221 1104 58-59 0.1193570610697563 1104 60-61 0.24300819493733172 1104 62-63 0.09833333639053166 1104 64-65 0.021397764325378432 1104 66-67 0.05841127549460623 1104 68-69 -0.020669420871904265 1104 70-71 -0.04237200471439451 1104 72-73 -0.037851729771446685 1104 74-75 -0.024697022976845062 1104 76-77 -0.00702923706564107 1104 78-79 0.027984574002982043 1104 80-81 0.08977679850868725 1104 82-83 0.2066291778782734 1104 84-85 0.19498798073833612 1104 86-87 0.24615621272347354 1104 88-89 0.24261284384672877 1104 90-91 0.015621424860039923 1104 92-93 0.04691777765427929 1104 94-95 0.14185002573382732 1104 96-97 0.25537224200684605 1104 98-99 0.3556272461635679 1104 100-101 0.5561296495749346 1105 1 0.09255095913695754 1105 2 0.19154489226503557 1105 3 0.11771858467455587 1105 4 0.09420748948412694 1105 5 0.06954205008782566 1105 6 0.10679805119563213 1105 7 0.1186593414748387 1105 8 0.09759682178382434 1105 9 0.20793528110617387 1105 10-11 0.08089897547608871 1105 12-13 0.013914547227436458 1105 14-15 0.1606828238144189 1105 16-17 0.24245291233376776 1105 18-19 0.15450152824084995 1105 20-21 0.13785170653255108 1105 22-23 0.17565483807948823 1105 24-25 0.15636077611546995 1105 26-27 0.12565136909223895 1105 28-29 0.17786425357797242 1105 30-31 0.2225767110255248 1105 32-33 0.1232852491052725 1105 34-35 0.020897729720843472 1105 36-37 0.006129760676742535 1105 38-39 0.07376574734477259 1105 40-41 0.20038896497058545 1105 42-43 0.043230101972653756 1105 44-45 -0.05741408560279382 1105 46-47 -0.08160438556718219 1105 48-49 -0.03634249799666378 1105 50-51 -0.1321136784896595 1105 52-53 -0.1014387549116762 1105 54-55 -0.18353529496164356 1105 56-57 -0.224148412279213 1105 58-59 -0.42948668266236467 1105 60-61 -0.29028740750226234 1105 62-63 -0.2598378530365437 1105 64-65 -0.0904986200746265 1105 66-67 -0.028014057360230993 1105 68-69 0.0037072548699654817 1105 70-71 -0.1109253060101949 1105 72-73 -0.13581983794713182 1105 74-75 -0.1318588322968708 1105 76-77 -0.0326414983918788 1105 78-79 0.08701633361176775 1105 80-81 0.017701122657989288 1105 82-83 -0.01897503359101904 1105 84-85 0.13569089064919382 1105 86-87 0.1103375872432899 1105 88-89 0.2680735520002884 1105 90-91 -0.03407542382333517 1105 92-93 -0.025313723639545316 1105 94-95 -0.20490947961820183 1105 96-97 0.1703715989177148 1105 98-99 0.3150089721788163 1105 100-101 0.7872702738300958 1106 1 -0.035213984965253076 1106 2 0.07269792671632302 1106 3 0.05189894059515865 1106 4 0.10531717463128842 1106 5 0.00837675234655677 1106 6 -0.1256768518388256 1106 7 0.031598538371937934 1106 8 0.059887205082929995 1106 9 0.04636786153054118 1106 10-11 -0.2256718664130304 1106 12-13 -0.19250656844675973 1106 14-15 -0.1508895866350457 1106 16-17 -0.07149087616725325 1106 18-19 -0.033076899776496305 1106 20-21 0.02070662896010589 1106 22-23 -0.018292657927808875 1106 24-25 0.16480390867308614 1106 26-27 0.12065906929758086 1106 28-29 0.08157033388825852 1106 30-31 0.10954910436342402 1106 32-33 0.03428584801012846 1106 34-35 0.10083370393941493 1106 36-37 0.26665123569384974 1106 38-39 0.3084936734228023 1106 40-41 0.09183460722474734 1106 42-43 -0.253970730790293 1106 44-45 -0.10506195806457441 1106 46-47 0.15963876980585212 1106 48-49 0.17834279175920642 1106 50-51 0.17974321176815522 1106 52-53 0.2493227098384878 1106 54-55 0.17919143330666287 1106 56-57 0.16804158315770223 1106 58-59 0.11693699974693317 1106 60-61 0.17460678597252866 1106 62-63 0.2023562776958272 1106 64-65 0.2557111677419357 1106 66-67 0.2910054498266277 1106 68-69 0.24052564410108346 1106 70-71 0.11733516056521154 1106 72-73 0.21205975365678853 1106 74-75 0.09949811499950556 1106 76-77 0.28371074322344825 1106 78-79 0.22221411481185882 1106 80-81 0.23751916965776232 1106 82-83 0.00769548317831692 1106 84-85 -0.06189851729057594 1106 86-87 -0.0030507882985766344 1106 88-89 0.022727185356437474 1106 90-91 -0.09191252170149866 1106 92-93 -0.10017897004611243 1106 94-95 -0.48686675773821264 1106 96-97 -0.04721481449775311 1106 98-99 0.1851963438420583 1106 100-101 0.6975015651830532 1107 1 0.007637322224290699 1107 2 0.007528928405392321 1107 3 -0.0034353841459733303 1107 4 -0.007542210695881124 1107 5 -0.02452972485106386 1107 6 -0.10672535738336109 1107 7 -0.05620255584852174 1107 8 -0.07375318927705621 1107 9 -0.013788560588871235 1107 10-11 0.07309438165923865 1107 12-13 -0.057582851769076626 1107 14-15 -0.04483626174704369 1107 16-17 -0.013751699035729814 1107 18-19 -0.039881353767832195 1107 20-21 -0.03067744058531474 1107 22-23 -0.04776477917331334 1107 24-25 -0.02158029856786925 1107 26-27 -0.11668258084562666 1107 28-29 -0.18001858070379484 1107 30-31 -0.067354765798882 1107 32-33 -0.17826716476801607 1107 34-35 0.007832997102376282 1107 36-37 -0.1969152161677954 1107 38-39 0.02394586553560174 1107 40-41 -0.0701536243974914 1107 42-43 -0.19578105979080362 1107 44-45 -0.14858040008558504 1107 46-47 -0.19419329672862773 1107 48-49 -0.13753327308621266 1107 50-51 -0.13149503821348674 1107 52-53 -0.20056437982884034 1107 54-55 -0.039591613619975874 1107 56-57 -0.08877205053547499 1107 58-59 -0.25245603836717834 1107 60-61 -0.15577940408402213 1107 62-63 -0.22758079544803422 1107 64-65 -0.37156080846428097 1107 66-67 -0.1042165153639445 1107 68-69 -0.20336985417313258 1107 70-71 -0.31747312136178607 1107 72-73 -0.2082783347620989 1107 74-75 -0.17853394051933336 1107 76-77 -0.10218028453596517 1107 78-79 -0.11451042987492954 1107 80-81 -0.1704295341683384 1107 82-83 -0.056147736693901606 1107 84-85 0.006965081519734184 1107 86-87 0.04798973090536052 1107 88-89 -0.09092509671744153 1107 90-91 -0.04983276845188911 1107 92-93 -0.03918053977513125 1107 94-95 0.07398961664674886 1107 96-97 -0.06977347279253365 1107 98-99 0.20212600896653754 1107 100-101 0.5827217862302732 1108 1 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2316 66-67 -0.835519992174234 2316 68-69 -0.5610087816571436 2316 70-71 -0.782168486840277 2316 72-73 -0.7226633669411662 2316 74-75 -0.7949553132023084 2316 76-77 -0.6872344378890993 2316 78-79 -0.7566727078128359 2316 80-81 -0.8215993440694191 2316 82-83 -1.0213458600730831 2316 84-85 -1.0863123271223998 2316 86-87 -1.2954334604273683 2316 88-89 -1.154294609238935 2316 90-91 -0.975375221140915 2316 92-93 -0.864437540550643 2316 94-95 -0.8703573068254613 2316 96-97 -0.9611944393360474 2316 98-99 -1.0123160537431062 2316 100-101 -1.473488983904197 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 6.0 5 28.0 6 50.0 7 135.0 8 352.0 9 763.0 10 1184.0 11 1873.0 12 2746.0 13 3959.0 14 5089.0 15 6859.0 16 8832.0 17 11559.0 18 10378.0 19 7416.0 20 6657.0 21 6804.0 22 7468.0 23 8372.0 24 9669.0 25 11553.0 26 14359.0 27 18035.0 28 22768.0 29 28855.0 30 36500.0 31 45901.0 32 58781.0 33 76892.0 34 104396.0 35 148328.0 36 224547.0 37 333640.0 38 264256.0 39 24538.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.01881767166184 19.88924637426317 15.482106156629715 22.609829797445276 2 15.707136976943008 20.528834782241002 39.69914519072329 24.064883050092696 3 19.61462888010603 24.133859776393837 29.7886960095246 26.46281533397553 4 13.258132844946397 17.10774772489122 37.06085147936579 32.573267950796605 5 13.78411650775421 37.30465794448846 34.49372797456167 14.417497573195655 6 32.471256393891466 36.665490626697164 17.005802237647284 13.857450741764085 7 28.74486143209955 32.444564250763435 20.492364329254194 18.318209987882824 8 26.63750351820946 35.402238704400375 19.344337896104136 18.615919881286032 9 25.997883128054895 16.828867684932288 20.010782568709143 37.162466618303675 10-11 24.885930729924404 26.98489779684108 27.243956938437613 20.88521453479691 12-13 25.741398704664498 23.885378830299818 27.9911067503795 22.382115714656187 14-15 22.45162709754657 25.483795094195514 26.033626814768418 26.030950993489494 16-17 22.048073606888156 28.750312179149205 26.89230116665808 22.309313047304556 18-19 22.090457294553083 27.204711559680067 29.241969563028476 21.462861582738377 20-21 22.540127408734673 26.40206428049478 29.55692363800697 21.500884672763576 22-23 22.21753490655357 26.247356364974582 29.473235280709275 22.061873447762565 24-25 22.238868144802566 26.453692823595908 29.50615126485704 21.801287766744487 26-27 21.965619627653883 26.783701499400742 29.23917338051638 22.011505492429 28-29 21.918347201795036 26.92199436275773 29.3434581693102 21.816200266137034 30-31 22.118966353364332 26.688575366323235 29.282139917042933 21.910318363269496 32-33 21.923003738221432 26.62230749613162 29.186900500411618 22.267788265235332 34-35 22.31978508860602 26.81790672813788 29.132426064745626 21.729882118510474 36-37 22.45047087847543 26.421984283348994 29.141774921806558 21.985769916369023 38-39 22.072684555688134 26.745296170470294 29.1090704395086 22.072948834332966 40-41 22.34399961943875 26.6328786419249 29.21263463345213 21.810487105184222 42-43 22.195879030566143 26.665477258347746 28.94276468051997 22.195879030566143 44-45 22.23643821099214 26.85520305188979 28.866099260416213 22.042259476701854 46-47 22.63259189959526 26.99946615713744 28.3914217794674 21.976520163799904 48-49 22.46569993538387 27.16800986287903 28.259777979510474 22.106512222226627 50-51 22.531307108963404 27.200615240685167 28.226016382633194 22.042061267718232 52-53 22.731696391407244 27.16351712591688 28.135996469237305 21.968790013438568 54-55 22.559584923960426 27.279370276845093 28.09668502081855 22.064359778375927 56-57 22.524435864197777 27.409329300441215 27.964710872555585 22.101523962805423 58-59 22.425133163402165 27.16715095728332 28.14812025206897 22.25959562724554 60-61 22.262139309202052 27.375501633902722 28.255384347040145 22.10697470985508 62-63 22.438809583272217 27.226052060250243 28.377381976460704 21.957756380016836 64-65 22.2675239865905 27.21012927189912 28.294894004442522 22.227452737067853 66-67 22.14252018758498 27.43935796146025 28.07590611236864 22.342215738586134 68-69 22.545677260276147 27.06659293292476 28.23301976672124 22.15471004007786 70-71 22.397912462984472 27.15889224963232 28.057274467907984 22.38592081947522 72-73 22.34548618681593 27.30150361334977 28.03144123037566 22.32156896945864 74-75 22.33755782747097 27.480717569376452 27.78804059848542 22.39368400466716 76-77 22.432896348594102 27.27966759032053 28.0309787427472 22.256457318338164 78-79 22.412943310909288 27.43691338399555 27.914993452496574 22.235149852598585 80-81 22.501509691758603 27.3670777520987 27.713613125134618 22.417799431008078 82-83 22.40008589078656 27.426910244128045 27.862872055762942 22.310131809322453 84-85 22.366799281284493 27.543030927296954 27.709793010858363 22.380376780560194 86-87 22.516078052054965 27.467140253998206 27.599477785397813 22.41730390854902 88-89 22.44601117634389 27.512166728111453 27.486696873715772 22.555125221828888 90-91 22.26178692677615 27.529446668261293 27.68446043184482 22.524305973117734 92-93 22.425484147397786 27.259921627287603 27.826673546798 22.487920678516613 94-95 22.20370069386358 27.11274259127854 27.912251561556445 22.771305153301434 96-97 22.479706703559966 26.957280678456137 27.92216201073764 22.640850607246257 98-99 22.122174237216242 27.149621271558978 27.742234076607236 22.98597041461754 100-101 21.96475568909044 27.100845194759543 28.01776686474439 22.91663225140563 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 130.0 1 101.5 2 109.0 3 154.5 4 127.5 5 119.5 6 268.0 7 587.5 8 947.5 9 1187.5 10 1126.0 11 976.0 12 1055.5 13 1175.0 14 1218.0 15 1329.5 16 1475.5 17 1658.0 18 1894.5 19 2055.0 20 2259.5 21 2649.0 22 3261.0 23 4048.0 24 4906.0 25 6127.5 26 7977.0 27 10296.0 28 12597.0 29 15041.5 30 17706.0 31 21014.5 32 25650.0 33 29117.0 34 31505.5 35 34294.5 36 38187.5 37 42008.5 38 44154.0 39 46256.5 40 48208.0 41 49782.5 42 50429.0 43 51379.5 44 53822.5 45 56503.0 46 57035.5 47 57651.5 48 58465.5 49 57305.5 50 55406.0 51 53765.0 52 52350.0 53 50880.5 54 50467.5 55 46958.5 56 41541.0 57 37548.5 58 34144.5 59 29855.5 60 24354.5 61 20581.0 62 17183.5 63 12028.0 64 7768.5 65 5542.0 66 4093.5 67 3304.5 68 2559.5 69 1907.5 70 1417.5 71 845.0 72 534.5 73 425.0 74 297.0 75 192.0 76 132.0 77 76.0 78 51.5 79 32.5 80 14.5 81 8.0 82 5.0 83 4.5 84 3.0 85 1.0 86 1.0 87 1.5 88 0.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05463960981900877 2 0.0 3 0.0 4 0.0 5 0.01631920631837384 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003072239246171593 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.589055957038864E-4 24-25 2.3124381422796937E-4 26-27 0.00105711457932786 28-29 0.0031713437379835806 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012982688427370282 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.64278644831965E-4 84-85 0.0013544280547638206 86-87 0.0 88-89 0.0 90-91 0.009249752569118776 92-93 0.0011231842405358514 94-95 0.0 96-97 0.0 98-99 1.6517415301997815E-4 100-101 0.01156219071139847 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1513554.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.32773337420405 #Duplication Level Percentage of deduplicated Percentage of total 1 80.62537460644745 51.058222262724264 2 11.631690750034746 14.732172210187919 3 3.4334880869994735 6.523050543510256 4 1.4570771945881031 3.6909358433803447 5 0.7691632086397063 2.43546812989913 6 0.4555904117395351 1.7310904873491073 7 0.31945092052330637 1.4161071904730798 8 0.22722561277387412 1.1511746417227227 9 0.16740412340382627 0.9541191323393877 >10 0.8354042811421615 9.56741552124111 >50 0.05241439118321683 2.262882583690556 >100 0.02320761198280308 2.6807313160505624 >500 0.00167253369452537 0.6756229617770022 >1k 8.36266847262685E-4 1.1210071756546889 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4799 0.31706830413715004 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3779 0.24967724970499897 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2724 0.17997375713056818 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 6.606966120799125E-5 5 0.0 0.0 0.0 0.0 6.606966120799125E-5 6 0.0 0.0 0.0 0.0 6.606966120799125E-5 7 6.606966120799125E-5 0.0 0.0 0.0 1.321393224159825E-4 8 6.606966120799125E-5 0.0 0.0 0.0 1.321393224159825E-4 9 6.606966120799125E-5 0.0 0.0 0.0 1.321393224159825E-4 10-11 6.606966120799125E-5 0.0 0.0 0.0 1.321393224159825E-4 12-13 6.606966120799125E-5 0.0 0.0 0.0 2.973134754359607E-4 14-15 6.606966120799125E-5 0.0 0.0 0.0 4.624876284559388E-4 16-17 6.606966120799125E-5 0.0 0.0 0.0 4.624876284559388E-4 18-19 6.606966120799125E-5 0.0 0.0 6.606966120799125E-5 5.2855728966393E-4 20-21 6.606966120799125E-5 0.0 0.0 1.321393224159825E-4 5.946269508719214E-4 22-23 6.606966120799125E-5 0.0 0.0 1.6517415301997815E-4 6.606966120799126E-4 24-25 6.606966120799125E-5 0.0 0.0 2.64278644831965E-4 7.267662732879038E-4 26-27 6.606966120799125E-5 0.0 0.0 3.9641796724794757E-4 9.249752569118776E-4 28-29 2.312438142279694E-4 0.0 0.0 9.249752569118776E-4 9.249752569118776E-4 30-31 2.64278644831965E-4 0.0 0.0 0.0027749257707356326 9.249752569118776E-4 32-33 2.973134754359607E-4 0.0 0.0 0.005384677388451288 0.0011231842405358514 34-35 3.9641796724794757E-4 0.0 0.0 0.009183682907910785 0.0012553235629518338 36-37 4.624876284559388E-4 0.0 0.0 0.012883583935558295 0.0012553235629518338 38-39 4.624876284559388E-4 0.0 0.0 0.016187066995957858 0.0013213932241598252 40-41 4.624876284559388E-4 0.0 0.0 0.018995027597297487 0.0013213932241598252 42-43 4.624876284559388E-4 0.0 0.0 0.021902092690449102 0.001420497715971812 44-45 4.624876284559388E-4 0.0 0.0 0.02622965549957253 0.0014535325465758076 46-47 4.624876284559388E-4 0.0 0.0 0.031746472210439795 0.0014535325465758076 48-49 4.624876284559388E-4 0.0 0.0 0.037824881041575 0.0015526370383877946 50-51 4.624876284559388E-4 0.0 0.0 0.04334169775244227 0.0016517415301997814 52-53 4.624876284559388E-4 3.3034830603995626E-5 0.0 0.054903888463840735 0.0018499505138237552 54-55 4.624876284559388E-4 6.606966120799125E-5 0.0 0.07465871716503011 0.0019160201750317465 56-57 4.624876284559388E-4 6.606966120799125E-5 0.0 0.10993991625009744 0.0019160201750317465 58-59 4.624876284559388E-4 6.606966120799125E-5 0.0 0.1474344489856325 0.0019160201750317465 60-61 4.624876284559388E-4 6.606966120799125E-5 0.0 0.18621734011472335 0.0019160201750317465 62-63 4.624876284559388E-4 6.606966120799125E-5 0.0 0.23999804433802824 0.001982089836239738 64-65 4.624876284559388E-4 6.606966120799125E-5 0.0 0.30600163588481155 0.001982089836239738 66-67 5.2855728966393E-4 6.606966120799125E-5 0.0 0.39787149979452335 0.0020151246668437335 68-69 5.2855728966393E-4 6.606966120799125E-5 0.0 0.5108175856295845 0.002048159497447729 70-71 5.2855728966393E-4 6.606966120799125E-5 0.0 0.612829142534723 0.00211422915865572 72-73 5.2855728966393E-4 6.606966120799125E-5 0.0 0.7169218937679132 0.002411542634091681 74-75 5.2855728966393E-4 6.606966120799125E-5 0.0 0.8407694737022927 0.002576716787111659 76-77 5.2855728966393E-4 6.606966120799125E-5 0.0 0.9872789474310134 0.0027088561095276417 78-79 5.2855728966393E-4 6.606966120799125E-5 0.0 1.1152889160214965 0.002774925770735633 80-81 5.2855728966393E-4 6.606966120799125E-5 0.0 1.2535396820992182 0.0028079606013396282 82-83 5.2855728966393E-4 6.606966120799125E-5 0.0 1.412965774594101 0.002840995431943624 84-85 5.2855728966393E-4 6.606966120799125E-5 0.0 1.6281876959791326 0.002840995431943624 86-87 5.2855728966393E-4 6.606966120799125E-5 0.0 1.8800122096733913 0.0029731347543596066 88-89 5.2855728966393E-4 6.606966120799125E-5 0.0 2.1699919527152645 0.003039204415567598 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2770 0.0 45.821384 1 GTATCAA 4835 0.0 39.42617 1 TCAACGC 6095 0.0 30.94431 4 ATCAACG 6150 0.0 30.667574 3 CAACGCA 6185 0.0 30.417215 5 TATCAAC 6270 0.0 30.232172 2 AACGCAG 6420 0.0 29.297039 6 ACGCAGA 7460 0.0 24.76869 7 CGCAGAG 7610 0.0 24.093224 8 TAAGGTG 790 0.0 24.054533 5 GTAAGGT 685 0.0 23.580462 4 GCGTTAA 125 2.2443222E-4 22.81802 1 GCGTGCC 330 1.8189894E-12 21.590841 9 TATAGGG 420 0.0 21.491577 2 GGTAAGG 710 0.0 21.41192 3 GTACATG 5075 0.0 21.075758 1 GCAGAGT 8590 0.0 21.012741 9 AGGTAAG 730 0.0 20.825293 2 GAGTACT 5115 0.0 20.662203 12-13 TACATGG 4925 0.0 20.25709 2 >>END_MODULE