FastQCFastQC Report
Thu 26 May 2016
SRR937264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545356
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38580.2496512130538206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29380.1901180051716239No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21690.14035600858313554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20510.13272022757215815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25850.049.9964751
GTATCAA45150.038.517171
ATCAACG57700.029.8043173
TCAACGC58000.029.654974
TATCAAC58500.029.6403582
CAACGCA58550.029.2131545
AACGCAG61000.028.0380176
GTACATG54850.024.1689951
ACGCAGA71050.023.8030387
TACATGG53700.023.7086562
CGCAGAG70250.023.5992058
ACATGGG52800.022.1333753
GTGTAGC10150.021.0657771
GCAGAGT79150.020.2254059
GAGTACT44150.019.90357412-13
CATGGGG30300.019.6012744
AGAGTAC69750.019.40907310-11
AGGACCG3455.2750693E-1119.2802125
GTACTTT45550.019.03112814-15
TAGACAG9100.018.7959375
Produced by FastQC (version 0.11.5)