FastQCFastQC Report
Thu 26 May 2016
SRR937264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545356
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49270.3188262122125905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38480.24900411296814454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26860.17381108301258738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27750.047.6258961
GTATCAA50250.042.3841321
ATCAACG63000.033.3998833
TCAACGC63600.033.3088384
TATCAAC64750.032.6438982
CAACGCA65500.032.2701155
AACGCAG67700.031.289596
TACCGTG1554.5851266E-827.5834647
ACGCAGA78500.026.990027
CGCAGAG78650.026.9385458
GTACCGT950.001227403824.9977026
TGGTATC13600.024.4478652
TAGGTCG1201.7115042E-423.7493535
GTACATG51650.023.6550671
GCAGAGT88100.023.4019379
TACATGG51250.022.6140172
GGACCGA3600.022.4284936
CTAACGC1100.00287826521.590323
ACATGGG50700.021.3603653
GTGGTAT16300.020.9993691
Produced by FastQC (version 0.11.5)