FastQCFastQC Report
Thu 26 May 2016
SRR937263_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937263_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252569
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15020.5946889760817836No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12760.5052084776833261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12030.47630548483780666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.2878421342286662No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7070.2799235060518116No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5590.22132565754308725No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4500.17816913397922943No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4430.1753976141173303No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3810.1508498667690809No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3730.14768241549833908No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3590.14213937577454083No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3480.13778413027727077No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3350.13263702196231525No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3310.13105329632694432No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3190.12630211942083153No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3160.12511432519430335No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.11877942265281963No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA2980.11798755983513418No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC2690.10650554897869494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAA1001.2935907E-633.25091
CGACGAG404.489387E-429.68830794-95
GTGTAGC1303.0202864E-729.2315621
ACGAAGG350.00838606927.14359394-95
TAATACC908.9266885E-426.3896054
GAGTACT12900.026.1493112-13
CTGCACC950.001223705625.0006774
GTACTTT13550.024.8850414-15
GTAGGAC1551.4030193E-624.5167963
GTCTTAG1452.324514E-522.9316561
ATAGCCC1050.00219115622.6196613
TAGCCCT3059.094947E-1221.8038714
GTAGCCC1553.89006E-521.4521963
AGTACTT13100.021.21661812-13
CTATCCT1150.003714751520.6527354
CTGCCGC600.0047591119.79220494-95
ACTTTTT17700.019.72119916-17
AATCCGC857.940899E-519.55935558-59
GTTCAGA1507.6968165E-419.0005171
AGTACAT7850.018.758472
Produced by FastQC (version 0.11.5)