FastQCFastQC Report
Thu 26 May 2016
SRR937263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252569
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17650.6988189366074221No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.6184448606123476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10710.42404253887056603No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10180.4030581742019012No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7950.314765470029972No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6540.2589391413831468No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5320.21063550950433346No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4410.17460575129964487No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.16233187762552015No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3540.14015971873032715No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3420.13540854182421436No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3360.13303295337115797No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3210.127093982238517No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3120.1235305995589324No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA2990.11838349124397689No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC2970.11759162842629144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.11402824574670684No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG2880.11402824574670684No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA2750.10888113743175133No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2550.10096250925489669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC1502.5465852E-1134.832751
AAGGACC1001.2940473E-633.2494434
TCACGGA404.4905514E-429.68700836-37
CCGGGGA350.00838787727.14240592-93
ACAGTAT1109.449021E-525.9086598
ACTGTCC1309.982032E-625.5764988
TCCTACA2151.6461854E-924.301922
GACCTGG1401.772587E-523.7496057
GTACATA1000.001650247723.7496031
AATCACG500.001652527523.74960334-35
GTCTAGA1252.2493955E-422.799621
GGACCTG1903.2664684E-722.4996246
TCCTCGC652.938681E-421.92271244-45
TGTAGAC1954.195208E-721.9227122
CACGAGC550.002873783721.59268894-95
CACGGAA550.002875435221.5905536-37
ATCACGG550.002875435221.5905534-35
CTAGGCC1100.00287149821.590553
GAGTACT15150.021.4765412-13
GTAGACC1604.970173E-520.7809053
Produced by FastQC (version 0.11.5)