##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937263_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 252569 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.176735862279216 33.0 31.0 34.0 31.0 34.0 2 32.37518856233346 34.0 31.0 34.0 31.0 34.0 3 32.43523947911264 34.0 31.0 34.0 31.0 34.0 4 36.087041560919985 37.0 35.0 37.0 35.0 37.0 5 36.012448083494014 37.0 35.0 37.0 35.0 37.0 6 36.05598866052445 37.0 35.0 37.0 35.0 37.0 7 36.002328076683995 37.0 35.0 37.0 35.0 37.0 8 35.988894123981964 37.0 35.0 37.0 35.0 37.0 9 37.678978813710316 39.0 37.0 39.0 35.0 39.0 10-11 37.60549196457205 39.0 37.0 39.0 35.0 39.0 12-13 37.5161817166794 39.0 37.0 39.0 35.0 39.0 14-15 38.72154341981795 40.0 38.0 41.0 35.0 41.0 16-17 38.69121309424355 40.0 38.0 41.0 34.5 41.0 18-19 38.73805771888078 40.0 38.0 41.0 35.0 41.0 20-21 38.66911022334491 40.0 38.0 41.0 34.0 41.0 22-23 38.608396121455925 40.0 38.0 41.0 34.0 41.0 24-25 38.63987464811596 40.0 38.0 41.0 34.0 41.0 26-27 38.48599194675515 40.0 38.0 41.0 34.0 41.0 28-29 38.338679727124074 40.0 38.0 41.0 34.0 41.0 30-31 38.31539698062707 40.0 38.0 41.0 34.0 41.0 32-33 38.14303616041557 40.0 37.0 41.0 34.0 41.0 34-35 37.99961198721934 40.0 37.0 41.0 34.0 41.0 36-37 37.81911675621315 40.0 37.0 41.0 33.0 41.0 38-39 37.66083327724305 40.0 37.0 41.0 33.0 41.0 40-41 37.435829416911815 40.0 37.0 41.0 33.0 41.0 42-43 37.17392870859052 40.0 36.5 41.0 32.5 41.0 44-45 36.76209471471162 40.0 36.0 41.0 31.0 41.0 46-47 36.264098127640366 40.0 36.0 41.0 30.0 41.0 48-49 35.83830557194271 39.0 36.0 41.0 28.5 41.0 50-51 35.55503842514322 39.0 35.5 41.0 27.5 41.0 52-53 35.306951763676466 39.0 35.0 41.0 26.5 41.0 54-55 35.10148909802866 39.0 35.0 40.0 25.5 41.0 56-57 34.81314809022485 39.0 35.0 40.0 24.5 41.0 58-59 34.5841453226643 38.5 35.0 40.0 23.0 41.0 60-61 34.26421492740597 38.0 34.0 40.0 22.0 41.0 62-63 33.93535825853529 38.0 34.0 40.0 21.0 41.0 64-65 33.55102566031461 38.0 34.0 40.0 18.5 41.0 66-67 33.13086126959365 37.0 33.0 40.0 14.5 41.0 68-69 32.715364910182956 36.5 32.5 39.0 10.5 40.5 70-71 32.29788691407101 36.0 32.0 39.0 8.0 40.0 72-73 31.838321409199068 35.5 31.5 38.5 6.5 40.0 74-75 31.37670497962933 35.0 31.0 38.0 2.0 40.0 76-77 29.991099461929217 34.0 29.5 36.0 2.0 39.0 78-79 30.622326968076052 34.5 31.0 37.0 2.0 39.0 80-81 30.434726747938186 35.0 31.0 36.0 2.0 39.0 82-83 30.047030712399383 34.0 30.5 36.0 2.0 37.5 84-85 29.607798265028567 34.0 30.0 35.5 2.0 37.0 86-87 29.242852448241866 34.0 30.0 35.0 2.0 36.5 88-89 28.78675332285435 34.0 29.0 35.0 2.0 36.0 90-91 28.27452894060633 34.0 29.0 35.0 2.0 36.0 92-93 27.882614255906304 34.0 28.5 35.0 2.0 35.0 94-95 27.465997410608587 33.5 27.0 35.0 2.0 35.0 96-97 26.882515273054096 33.0 26.0 35.0 2.0 35.0 98-99 26.00536487058982 33.0 24.5 34.5 2.0 35.0 100-101 23.295341075112148 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33947714974168974 1101 2 0.32999921896685436 1101 3 0.3144540167976402 1101 4 0.15919874074898388 1101 5 0.11811402815567362 1101 6 0.24913288253349464 1101 7 0.27075731639981626 1101 8 0.3688881923842544 1101 9 0.20599705819825687 1101 10-11 0.43100283775793713 1101 12-13 0.36980054054645706 1101 14-15 0.4117834725855545 1101 16-17 0.2750563100803092 1101 18-19 0.33923189716583835 1101 20-21 0.3204754322761758 1101 22-23 0.35500973407238945 1101 24-25 0.44294539804065636 1101 26-27 0.3714826356155072 1101 28-29 0.25481298264153196 1101 30-31 0.4237729131709571 1101 32-33 0.7398388868033905 1101 34-35 0.7526271168558836 1101 36-37 0.8294698821100539 1101 38-39 0.7756884981641292 1101 40-41 0.8159046604286928 1101 42-43 0.9846675763891568 1101 44-45 0.9646441861537554 1101 46-47 1.0976047568967076 1101 48-49 1.2963504561790202 1101 50-51 1.3417291600408987 1101 52-53 1.3601023378400825 1101 54-55 1.396466811202913 1101 56-57 1.412715919863082 1101 58-59 1.5714680437992286 1101 60-61 1.5545757641816138 1101 62-63 1.5956278124069811 1101 64-65 1.4468525095549438 1101 66-67 1.2719463763048466 1101 68-69 1.3734160884887459 1101 70-71 1.4368302691369195 1101 72-73 1.6174654097667798 1101 74-75 1.5246800595022734 1101 76-77 1.6932677634053022 1101 78-79 1.7301213550316668 1101 80-81 1.3871718215208304 1101 82-83 1.2708728461868333 1101 84-85 1.1710704643503647 1101 86-87 1.4057257304965134 1101 88-89 1.7540069127649112 1101 90-91 1.7992598416834298 1101 92-93 1.795642364458459 1101 94-95 1.7196377640987421 1101 96-97 1.662886614703197 1101 98-99 2.008371576390733 1101 100-101 2.5314304443040427 1102 1 0.19968395053853527 1102 2 0.09645575026724629 1102 3 0.17473843257245392 1102 4 0.18744196884215114 1102 5 0.1054541628607808 1102 6 0.074602668747346 1102 7 0.18321624514064183 1102 8 0.16775328892865815 1102 9 0.3019205470777564 1102 10-11 0.19617009803545216 1102 12-13 0.12238123856381833 1102 14-15 0.3766756308856145 1102 16-17 0.31032800562262963 1102 18-19 0.3914107220750722 1102 20-21 0.24414110144664392 1102 22-23 0.06690724780592916 1102 24-25 -0.08227515785505801 1102 26-27 -0.008615159256734728 1102 28-29 0.04320868104697695 1102 30-31 0.06763227483088485 1102 32-33 -0.07260883088506631 1102 34-35 0.11411887673239107 1102 36-37 -0.08431281319941775 1102 38-39 -0.027320375815016007 1102 40-41 0.06872251532318074 1102 42-43 0.2916261903581301 1102 44-45 0.025968642633309003 1102 46-47 0.12955194421615346 1102 48-49 0.14523840602250715 1102 50-51 0.08559542080996607 1102 52-53 0.26487738376227554 1102 54-55 -0.10764247068606636 1102 56-57 0.29101563441244593 1102 58-59 0.6998992726168041 1102 60-61 0.9756456435334471 1102 62-63 0.5371923578850542 1102 64-65 0.7113169230875442 1102 66-67 0.5902540248297328 1102 68-69 0.38434686455229183 1102 70-71 0.41359751906576747 1102 72-73 0.7089993404479813 1102 74-75 0.116515826208893 1102 76-77 0.2036078032476567 1102 78-79 0.3925220725390197 1102 80-81 0.34523386175988335 1102 82-83 0.551231082429581 1102 84-85 0.4593195566863173 1102 86-87 0.04889086929796349 1102 88-89 0.38041584021215513 1102 90-91 0.9472026135730118 1102 92-93 0.6474839954078639 1102 94-95 0.6905321473615018 1102 96-97 0.92646276793241 1102 98-99 1.6412915381006776 1102 100-101 2.3466636773128258 1103 1 0.326707708542024 1103 2 0.25963250936930393 1103 3 0.24812081325534052 1103 4 0.1522099926334448 1103 5 0.375070328512102 1103 6 0.13942950497136763 1103 7 0.34382966991066866 1103 8 0.25684857616636236 1103 9 0.34445414229286797 1103 10-11 0.2131535750708622 1103 12-13 0.019809026883450542 1103 14-15 0.29398313113529184 1103 16-17 0.3152216438928761 1103 18-19 0.405570092671077 1103 20-21 0.2817977034196275 1103 22-23 0.5084776507265829 1103 24-25 0.2565954215028867 1103 26-27 0.2629873130847784 1103 28-29 -0.044654874109099296 1103 30-31 -0.008990337994667641 1103 32-33 -0.14543082895423964 1103 34-35 0.11452945409293136 1103 36-37 0.36728899334097065 1103 38-39 0.0821650708820556 1103 40-41 0.008140161272336854 1103 42-43 -0.029289951990151053 1103 44-45 0.004929327076865775 1103 46-47 -0.2566848368573389 1103 48-49 -0.24075979631445676 1103 50-51 -0.19754760436840968 1103 52-53 -0.2658049514242009 1103 54-55 -0.054567024321890756 1103 56-57 -0.2229606408741276 1103 58-59 -0.3922254097405755 1103 60-61 -0.1391273737662857 1103 62-63 -0.23312289894773386 1103 64-65 0.06192224727721651 1103 66-67 0.19558931117969536 1103 68-69 0.06163305916596329 1103 70-71 0.001910489981362673 1103 72-73 -0.5317543464257497 1103 74-75 -0.19086790826961675 1103 76-77 -0.4756702463989164 1103 78-79 -0.589429168625248 1103 80-81 -0.06361241585699062 1103 82-83 -0.060423766267113876 1103 84-85 0.044064943239355614 1103 86-87 0.272649982450865 1103 88-89 0.4675081757315347 1103 90-91 0.515791226154434 1103 92-93 0.11957583157639817 1103 94-95 0.4655468504696856 1103 96-97 0.3370012900758468 1103 98-99 0.6930242855290203 1103 100-101 1.2761164997547638 1104 1 -0.11345183008950244 1104 2 -0.11031116702598354 1104 3 -0.03202848472078301 1104 4 -0.013796421250724222 1104 5 -0.08450603174337346 1104 6 -0.27015628746671894 1104 7 -0.027973493912867298 1104 8 -0.14958417237164667 1104 9 -0.2396539774689046 1104 10-11 -0.18330800899683197 1104 12-13 0.1206121098597066 1104 14-15 -0.16921114487732325 1104 16-17 -0.06605412617746254 1104 18-19 -0.06491391304213323 1104 20-21 0.036379049257348584 1104 22-23 -0.24689195608615222 1104 24-25 -0.26759138961092077 1104 26-27 -0.18143691953979157 1104 28-29 -0.24449587445943877 1104 30-31 -0.07279231605810565 1104 32-33 -0.08034876896555687 1104 34-35 0.2770998585988238 1104 36-37 0.26674866402304787 1104 38-39 0.40036648840435873 1104 40-41 0.2800228249207066 1104 42-43 0.5139835543563578 1104 44-45 0.39980765192123613 1104 46-47 0.4645806925575968 1104 48-49 0.698422837689904 1104 50-51 0.5597219135123197 1104 52-53 0.5382077685477711 1104 54-55 0.5228307712422904 1104 56-57 0.7884725119002809 1104 58-59 0.8607794141471032 1104 60-61 0.722549668301248 1104 62-63 0.8308677227678487 1104 64-65 0.8132629646620728 1104 66-67 0.8055348740115136 1104 68-69 0.7493402303196532 1104 70-71 0.9128235252577284 1104 72-73 0.39895953505213555 1104 74-75 0.24101825876086025 1104 76-77 0.28366087710877963 1104 78-79 0.5649015506460522 1104 80-81 0.8411427516316223 1104 82-83 0.4844464738493066 1104 84-85 0.8069533470445691 1104 86-87 0.8187935672192381 1104 88-89 1.1185847920034 1104 90-91 0.9779412248069761 1104 92-93 1.0708586084109584 1104 94-95 1.315366291545491 1104 96-97 1.3062725865967195 1104 98-99 0.9381734487596773 1104 100-101 1.1438772996038509 1105 1 -0.12419757858090463 1105 2 0.06166921310889961 1105 3 0.10222883239174507 1105 4 0.015117064179591466 1105 5 0.11248322250813203 1105 6 -0.1255727255576531 1105 7 -0.048835735353378595 1105 8 -0.016875791978606003 1105 9 -2.6258052159988665E-4 1105 10-11 0.035172742237477905 1105 12-13 0.12642062255776665 1105 14-15 0.04401774279249793 1105 16-17 -0.3327778023206136 1105 18-19 0.022657483259784783 1105 20-21 0.3052567155260206 1105 22-23 0.3230360828954204 1105 24-25 0.3066284948642135 1105 26-27 0.4993772403233834 1105 28-29 0.09507623177180591 1105 30-31 -0.03810258744471895 1105 32-33 0.20347775012185565 1105 34-35 0.2801654625227883 1105 36-37 -0.0022096760330967413 1105 38-39 0.3720338542438455 1105 40-41 0.17775266454121663 1105 42-43 0.053408834294401686 1105 44-45 0.22141370568224517 1105 46-47 0.33522834172342897 1105 48-49 0.31316516561272323 1105 50-51 -0.32054205112029877 1105 52-53 0.13842074734670007 1105 54-55 0.012794952080646738 1105 56-57 0.14197698122811175 1105 58-59 -0.23350404127256041 1105 60-61 -0.42602724608772746 1105 62-63 0.1254077208316815 1105 64-65 -0.18951221362699755 1105 66-67 0.005194430628371549 1105 68-69 4.8579810177074023E-4 1105 70-71 0.049428449585711576 1105 72-73 0.20965042536805356 1105 74-75 -0.05549017971730663 1105 76-77 -0.20506092740249926 1105 78-79 0.21657633847398827 1105 80-81 -8.576356025571386E-4 1105 82-83 -0.1038267844036227 1105 84-85 0.4119099445469061 1105 86-87 0.5037866292735167 1105 88-89 0.5862184684692409 1105 90-91 0.35826972846956906 1105 92-93 0.5531829815756311 1105 94-95 0.46683132752600187 1105 96-97 0.7604095384183331 1105 98-99 0.7348351674340492 1105 100-101 1.3752981263488238 1106 1 -0.22293386851891128 1106 2 -0.2301524368672574 1106 3 -0.13198671362637526 1106 4 0.18357199980190586 1106 5 -0.012743291559587533 1106 6 0.12061230067028816 1106 7 -0.1271380720248203 1106 8 -0.2653736460558562 1106 9 0.07312797741831645 1106 10-11 -0.30924785862089266 1106 12-13 -0.4519651666649409 1106 14-15 -0.2509989477177541 1106 16-17 -0.1469647360354358 1106 18-19 0.25440521811910344 1106 20-21 0.02480845610780591 1106 22-23 0.20659593029646572 1106 24-25 0.13589649677170001 1106 26-27 0.23736007294140649 1106 28-29 0.36170713305936175 1106 30-31 0.04414586272218202 1106 32-33 0.2793003538414567 1106 34-35 -0.11020172870276213 1106 36-37 0.3864646205811155 1106 38-39 0.08324033969925893 1106 40-41 0.20867779568093425 1106 42-43 0.10278890105644223 1106 44-45 0.02553864607327938 1106 46-47 0.1693008262250899 1106 48-49 -0.017283093805502858 1106 50-51 -0.4712931240816687 1106 52-53 -0.6049626575173903 1106 54-55 -0.4972193540709995 1106 56-57 -0.41202874123254674 1106 58-59 -0.12251214809182898 1106 60-61 -0.31502343111395703 1106 62-63 -0.20507630396565446 1106 64-65 0.12957457702631814 1106 66-67 -0.20342502573786447 1106 68-69 -0.06910170785913294 1106 70-71 -0.25749811216917706 1106 72-73 -0.042686246067326294 1106 74-75 0.050709152662282264 1106 76-77 -0.01339426073583283 1106 78-79 -0.10197397149262954 1106 80-81 -0.20599592840179426 1106 82-83 -0.5945635512133514 1106 84-85 -0.6012755585527607 1106 86-87 -0.15378370930540797 1106 88-89 -0.030162075164522406 1106 90-91 -0.00867113943696296 1106 92-93 0.21939662010686334 1106 94-95 0.26411315871341046 1106 96-97 -0.04919942677003064 1106 98-99 0.08522858660428767 1106 100-101 0.6947544925863056 1107 1 0.15421099786646408 1107 2 0.22814573472205524 1107 3 0.17806954516405682 1107 4 0.03483782258671653 1107 5 -0.12976157566647828 1107 6 0.24754619659152866 1107 7 0.07905039492314359 1107 8 -0.016006557448264402 1107 9 -0.12476231794033765 1107 10-11 0.1981361076238386 1107 12-13 0.13541176723098403 1107 14-15 -1.4418885129430237E-5 1107 16-17 0.2673109320376952 1107 18-19 0.2320071871767695 1107 20-21 0.21580704963804465 1107 22-23 0.3627492355004094 1107 24-25 0.02559410577310217 1107 26-27 0.2244908746291685 1107 28-29 0.36961852546442486 1107 30-31 0.4099334857882795 1107 32-33 0.47339318084568305 1107 34-35 0.13002330645898041 1107 36-37 0.1703605418188019 1107 38-39 0.2559548263377991 1107 40-41 0.011701705666872897 1107 42-43 -0.10557959402092365 1107 44-45 -0.38339243690843006 1107 46-47 -0.37240100218559036 1107 48-49 -1.0020228968997316 1107 50-51 -0.8146475544614162 1107 52-53 -0.955701054141862 1107 54-55 -0.501438763353697 1107 56-57 -0.4552776863802279 1107 58-59 -0.3500086319174187 1107 60-61 -0.39986871944025637 1107 62-63 -0.7232549256252909 1107 64-65 -0.46518632311433095 1107 66-67 -0.3723068822779467 1107 68-69 -0.07856021700118987 1107 70-71 -0.4074629504251561 1107 72-73 -0.19746824325438794 1107 74-75 0.13144051693794978 1107 76-77 0.18101502196459052 1107 78-79 0.24542405944970724 1107 80-81 0.011338811401298443 1107 82-83 0.02600606903981273 1107 84-85 0.10329546817016677 1107 86-87 0.34941600940064177 1107 88-89 0.710555224413536 1107 90-91 0.1740644442479855 1107 92-93 -9.268679381406741E-4 1107 94-95 0.4860540869834544 1107 96-97 0.6979876337081699 1107 98-99 1.4342792195156804 1107 100-101 1.5031933111517048 1108 1 -0.31203372787193473 1108 2 -0.19168548890663573 1108 3 0.22237334263240882 1108 4 0.06648339220696897 1108 5 0.03479538635883728 1108 6 -0.15118798062366068 1108 7 -0.0032280860895141927 1108 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-0.054075718695649755 2316 62-63 0.1264596437467631 2316 64-65 -0.23790908310440528 2316 66-67 0.12451615073272393 2316 68-69 0.2826956777617866 2316 70-71 0.3962600577654669 2316 72-73 0.4040457800764621 2316 74-75 0.3128006559302534 2316 76-77 0.528272405930835 2316 78-79 0.44050968863809103 2316 80-81 0.4605073395720609 2316 82-83 0.5078874291788047 2316 84-85 0.1304237878524681 2316 86-87 0.5901705390606082 2316 88-89 0.9316026307178298 2316 90-91 1.1676360501055179 2316 92-93 1.2667822410219003 2316 94-95 1.0582308057722365 2316 96-97 0.7962254098312762 2316 98-99 0.9808168218984079 2316 100-101 1.2810290023902269 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 4.0 6 8.0 7 17.0 8 55.0 9 119.0 10 232.0 11 438.0 12 712.0 13 1097.0 14 1348.0 15 2092.0 16 3015.0 17 4081.0 18 3545.0 19 1986.0 20 1543.0 21 1480.0 22 1389.0 23 1553.0 24 1677.0 25 1895.0 26 2209.0 27 2595.0 28 3162.0 29 4052.0 30 5200.0 31 6266.0 32 8289.0 33 10843.0 34 14813.0 35 21472.0 36 33387.0 37 55175.0 38 51852.0 39 4965.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.04397214806835 21.534549544910845 16.33370036201174 21.08777794500907 2 15.102407658897175 22.433869556438044 40.292355752289474 22.17136703237531 3 18.625405334779803 25.452450617455032 32.96168571756629 22.960458330198875 4 11.965047175227363 19.082706111993158 39.7542849676722 29.197961745107275 5 11.577171907540999 38.908641701575036 36.51085260882304 13.003333782060928 6 28.26395955164727 39.253431735486146 18.499499146767814 13.98310956609877 7 24.828066785710043 35.25452450617455 22.51305583820659 17.404352869908816 8 24.01284401490286 35.140892191836684 21.746136699278217 19.100127093982238 9 24.240108643578587 17.394058653278908 23.15486065194066 35.21097205120185 10-11 22.596597365472405 27.805866911616235 29.681789926713098 19.915745796198266 12-13 24.406178358324194 26.08499400140165 29.559429677817857 19.9493979624563 14-15 21.095621394549607 27.84506412109166 29.532325819874966 21.526988664483763 16-17 18.85722317465722 30.815539515934258 30.48612458377711 19.84111272563141 18-19 18.491184587182115 29.486199810744786 33.46333081257003 18.559284789503067 20-21 19.66460517563587 27.818844825947863 33.39496690000317 19.1215830984131 22-23 19.170594675065775 28.138133917205966 33.29816107836946 19.393110329358805 24-25 19.54119468343304 28.0511464193943 33.51836527839917 18.889293618773483 26-27 19.129088583838488 27.98569069895432 34.18236242046539 18.702858296741802 28-29 18.634890530312777 28.991303005046355 33.67002100503448 18.70378545960639 30-31 19.29701960861948 28.167123640214992 33.6316034327457 18.904253318419826 32-33 18.914670673500456 28.38715041661965 33.55347163244823 19.144707277431667 34-35 19.745099358987048 28.426489394977217 32.9488179467789 18.879593299256836 36-37 19.46066116716851 28.023882629860807 33.52456858238458 18.990887620586097 38-39 19.220726258420985 28.692018204962217 33.274537402724405 18.81271813389239 40-41 19.48160700640221 28.668007554371282 33.008405623809736 18.841979815416778 42-43 19.052493199285678 28.86631003337993 32.491971791389325 19.589224975945072 44-45 19.75836306118328 28.56486742236775 32.13557483301593 19.54119468343304 46-47 20.043433675550048 28.854095316527367 31.671543221852243 19.43092778607034 48-49 19.74806884455337 28.739671139371815 31.82160122580364 19.690658790271172 50-51 19.5380272321623 28.89566811445585 31.76755658849661 19.79874806488524 52-53 19.763708135202656 29.72712407302559 30.479393749826777 20.02977404194497 54-55 19.806270761653252 28.684438707838254 31.0243933340988 20.484897196409694 56-57 20.287723354806015 28.99979807498149 30.407928130530664 20.30455043968183 58-59 20.47697856823284 28.96238255684585 30.356655013085533 20.203983861835777 60-61 19.791225368117228 28.500330602726383 31.484267665469634 20.224176363686755 62-63 19.764499998020344 28.329288234106325 31.298377869017973 20.60783389885536 64-65 19.417268152465265 28.620693751014574 31.13109684878192 20.83094124773824 66-67 19.567326156416662 28.043623722626293 30.849391651390313 21.53965846956673 68-69 19.408161730061884 27.92029108877178 31.2449271288242 21.426620052342134 70-71 19.816169046874318 27.69975729404638 31.13109684878192 21.352976810297385 72-73 19.209320280717034 27.42059053520346 31.799221990161048 21.57086719391846 74-75 18.913247469008468 28.36630782083312 30.94916636641868 21.77127834373973 76-77 18.831091701673603 27.731431806753797 31.188704868768536 22.248771622804064 78-79 18.661039161575648 27.84051090988997 31.08081355985889 22.417636368675492 80-81 18.78595552106553 27.58216566562009 30.72843460598886 22.903444207325524 82-83 18.30909238483817 27.40622840932262 31.32160186246503 22.963077343374174 84-85 18.30057984269614 27.074437231879667 31.040094073488973 23.58488885193522 86-87 18.212646841061257 26.953030656968984 30.926598276114646 23.907724225855112 88-89 18.44664230368731 26.496323776868895 30.472464950172036 24.584568969271764 90-91 17.850955951209567 25.887202310834112 31.036093914262352 25.22574782369397 92-93 17.330020687538976 26.00017817019213 31.601849010660516 25.067952131608383 94-95 16.891621695457477 26.68973627008857 31.248490511503785 25.170151522950164 96-97 16.947843955513147 27.426960553353734 30.612228737493517 25.0129667536396 98-99 17.229979193763274 27.62062569164405 29.265921918212285 25.883473196380386 100-101 16.197841185368212 26.36252405502364 30.122631124626803 27.31700363498135 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 137.0 1 94.0 2 63.5 3 71.0 4 55.5 5 49.0 6 94.0 7 159.5 8 202.0 9 225.5 10 215.0 11 203.0 12 222.0 13 233.5 14 247.0 15 273.0 16 314.0 17 376.5 18 451.0 19 534.0 20 623.5 21 730.5 22 917.5 23 1127.5 24 1361.0 25 1706.0 26 2160.0 27 2851.0 28 3553.0 29 4310.0 30 5081.0 31 5695.0 32 6596.0 33 7336.0 34 8204.5 35 9291.0 36 9980.0 37 10455.0 38 10347.5 39 10072.5 40 10027.0 41 9927.5 42 9804.5 43 9650.5 44 9549.5 45 9476.5 46 9551.5 47 10507.5 48 12164.5 49 11143.0 50 8483.0 51 6982.0 52 5758.0 53 4812.5 54 4040.5 55 3412.5 56 2775.5 57 2114.0 58 1651.0 59 1260.0 60 913.5 61 675.0 62 456.5 63 295.0 64 190.5 65 118.5 66 78.5 67 63.0 68 45.0 69 25.5 70 22.5 71 15.0 72 7.5 73 6.0 74 4.0 75 3.0 76 2.5 77 2.5 78 1.0 79 0.5 80 1.0 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03602975820468862 2 0.0 3 0.0 4 0.0 5 0.0011877942265281962 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00395931408842732 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.959314088427321E-4 22-23 9.8982852210683E-4 24-25 0.0 26-27 0.0023755884530563924 28-29 0.004157279792848687 30-31 5.938971132640981E-4 32-33 1.9796570442136605E-4 34-35 0.0 36-37 1.9796570442136605E-4 38-39 1.9796570442136605E-4 40-41 0.0 42-43 0.008710490994540106 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.938971132640981E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.9796570442136605E-4 84-85 1.9796570442136605E-4 86-87 0.0 88-89 0.0 90-91 0.007720662472433275 92-93 5.938971132640981E-4 94-95 0.0 96-97 0.0 98-99 1.9796570442136605E-4 100-101 0.009106422403382837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 252569.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.95584547685873 #Duplication Level Percentage of deduplicated Percentage of total 1 88.97592977752433 69.3619383289652 2 8.280931569895172 12.910940437343784 3 1.5909919388568867 3.7208136522136592 4 0.4773539253977752 1.4885011538432367 5 0.19589232512617705 0.7635475913819414 6 0.11424548353051046 0.5343661956320088 7 0.05912615956501032 0.32264608310830256 8 0.04194140408058347 0.2615662092470766 9 0.026525124233767003 0.18610096374198193 >10 0.19027221138068337 2.9792173451467474 >50 0.024412215865620396 1.3464255113958759 >100 0.01881774972974905 3.1912187695010017 >500 0.0015257634916012743 0.7854148816475941 >1k 0.0020343513221350324 2.147302876831585 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1765 0.6988189366074221 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1562 0.6184448606123476 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1071 0.42404253887056603 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1018 0.4030581742019012 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 795 0.314765470029972 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 654 0.2589391413831468 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 532 0.21063550950433346 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 441 0.17460575129964487 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 410 0.16233187762552015 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 354 0.14015971873032715 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 342 0.13540854182421436 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 336 0.13303295337115797 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 321 0.127093982238517 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 312 0.1235305995589324 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 299 0.11838349124397689 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 297 0.11759162842629144 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 288 0.11402824574670684 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 288 0.11402824574670684 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 275 0.10888113743175133 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 255 0.10096250925489669 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.9796570442136605E-4 0.0 22-23 0.0 0.0 0.0 5.938971132640981E-4 0.0 24-25 0.0 0.0 0.0 0.0017816913397922945 0.0 26-27 0.0 0.0 0.0 0.0029694855663204905 0.0 28-29 0.0 0.0 0.0 0.0067308339503264454 0.0 30-31 0.0 0.0 0.0 0.013263702196231525 0.0 32-33 0.0 0.0 0.0 0.02593350727919895 0.0 34-35 0.0 0.0 0.0 0.034841963978160424 0.0 36-37 0.0 0.0 0.0 0.04474024919922873 0.0 38-39 0.0 0.0 0.0 0.06829816802537128 0.0 40-41 0.0 0.0 0.0 0.1096730002494368 0.0 42-43 0.0 0.0 0.0 0.1965799444904165 0.0 44-45 0.0 0.0 0.0 0.270421152239586 0.0 46-47 0.0 0.0 0.0 0.464031611163682 0.0 48-49 0.0 0.0 0.0 0.6485356476843951 0.0 50-51 0.0 0.0 0.0 0.7799848754201821 0.0 52-53 0.0 0.0 0.0 0.9367737133219041 0.0 54-55 0.0 0.0 0.0 1.068618872466534 0.0 56-57 0.0 0.0 0.0 1.4318859400797406 0.0 58-59 0.0 0.0 0.0 1.8721616667128587 0.0 60-61 0.0 0.0 0.0 2.294818445652475 0.0 62-63 0.0 0.0 0.0 2.8168540082116174 0.0 64-65 0.0 0.0 0.0 3.500231619874173 0.0 66-67 0.0 0.0 0.0 4.458781560682427 0.0 68-69 0.0 0.0 0.0 5.57352644227914 0.0 70-71 0.0 0.0 0.0 6.326192050489173 0.0 72-73 0.0 0.0 0.0 6.930779311792025 0.0 74-75 0.0 0.0 0.0 7.792919954547074 0.0 76-77 0.0 0.0 0.0 8.956562365135863 0.0 78-79 0.0 0.0 0.0 10.02854665457756 0.0 80-81 0.0 0.0 0.0 11.030451084654095 0.0 82-83 0.0 0.0 0.0 12.214088031389442 0.0 84-85 0.0 0.0 0.0 14.11930997074067 0.0 86-87 0.0 0.0 0.0 16.337317723077653 0.0 88-89 0.0 0.0 0.0 18.579081359945203 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAC 150 2.5465852E-11 34.83275 1 AAGGACC 100 1.2940473E-6 33.249443 4 TCACGGA 40 4.4905514E-4 29.687008 36-37 CCGGGGA 35 0.008387877 27.142405 92-93 ACAGTAT 110 9.449021E-5 25.908659 8 ACTGTCC 130 9.982032E-6 25.576498 8 TCCTACA 215 1.6461854E-9 24.30192 2 GACCTGG 140 1.772587E-5 23.749605 7 GTACATA 100 0.0016502477 23.749603 1 AATCACG 50 0.0016525275 23.749603 34-35 GTCTAGA 125 2.2493955E-4 22.79962 1 GGACCTG 190 3.2664684E-7 22.499624 6 TCCTCGC 65 2.938681E-4 21.922712 44-45 TGTAGAC 195 4.195208E-7 21.922712 2 CACGAGC 55 0.0028737837 21.592688 94-95 CACGGAA 55 0.0028754352 21.59055 36-37 ATCACGG 55 0.0028754352 21.59055 34-35 CTAGGCC 110 0.002871498 21.59055 3 GAGTACT 1515 0.0 21.47654 12-13 GTAGACC 160 4.970173E-5 20.780905 3 >>END_MODULE