FastQCFastQC Report
Thu 26 May 2016
SRR937262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236273
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15190.6429003737202305No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11830.5006919961231288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11700.4951898862756219No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7590.3212385672505957No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6090.25775268439474674No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5040.2133125663956525No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4600.1946900407579368No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.1574449894825054No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3700.1565985110444274No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3660.15490555416827145No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3590.1519428796349985No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3180.1345900716543998No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3110.13162739712112684No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3060.1295112010259319No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA2980.12612528727361993No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2870.121469655864191No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC2720.1151210675786061No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA2550.10792600085494322No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2460.10411684788359228No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2370.10030769491224135No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTGC650.005835533629.2299866
GTCTGAG908.9277606E-426.388181
AATGACG458.941082E-426.3881874-75
GTTATAT908.9277606E-426.388181
AGGACGT950.001220826525.0099455
ACTGTTG950.001223853124.9993298
GAGTACT12750.024.77121512-13
CCCTACA1000.001650016923.7493652
GGATAGA1000.001650016923.7493651
GTACTTT13550.023.13100214-15
ATGACGA753.0289424E-522.1660774-75
CGAAATC652.9342613E-421.92714378-79
CTCGGTG652.942697E-421.9178494-95
TAGGACG1100.002864034121.5994974
GGACGTG1100.002871101721.590336
GACTGTT1100.002871101721.590337
GTAGGAC1553.890291E-521.4510383
GTACATG8950.020.697771
CTAAGCA1150.003715202720.651629
AGTACTT13350.020.27820412-13
Produced by FastQC (version 0.11.5)