##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937262_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 236273 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.924261341752974 33.0 31.0 34.0 30.0 34.0 2 32.02910616109331 33.0 31.0 34.0 30.0 34.0 3 32.05849166007119 34.0 31.0 34.0 30.0 34.0 4 35.67147325339755 37.0 35.0 37.0 35.0 37.0 5 35.70429968722622 37.0 35.0 37.0 35.0 37.0 6 35.76434040283909 37.0 35.0 37.0 35.0 37.0 7 35.72943163205275 37.0 35.0 37.0 35.0 37.0 8 35.7248733456637 37.0 35.0 37.0 35.0 37.0 9 37.377309298988884 39.0 37.0 39.0 35.0 39.0 10-11 37.286564694230826 39.0 37.0 39.0 34.0 39.0 12-13 37.184145035615586 39.0 37.0 39.0 34.0 39.0 14-15 38.39463036402805 40.0 38.0 41.0 34.0 41.0 16-17 38.38620578737308 40.0 38.0 41.0 34.0 41.0 18-19 38.40754127640484 40.0 38.0 41.0 34.0 41.0 20-21 38.385433375798335 40.0 38.0 41.0 34.0 41.0 22-23 38.253410250007406 40.0 38.0 41.0 34.0 41.0 24-25 38.111991213553814 40.0 38.0 41.0 34.0 41.0 26-27 37.961688385892586 40.0 38.0 41.0 33.5 41.0 28-29 38.00419430066067 40.0 38.0 41.0 34.0 41.0 30-31 37.855565384110754 40.0 37.5 41.0 33.5 41.0 32-33 37.62531478416916 40.0 37.0 41.0 33.0 41.0 34-35 37.48245038578255 40.0 37.0 41.0 33.0 41.0 36-37 37.453773812496564 40.0 37.0 41.0 33.0 41.0 38-39 37.332380339691795 40.0 37.0 41.0 33.0 41.0 40-41 37.146893635751866 40.0 36.5 41.0 32.5 41.0 42-43 36.88324946142809 40.0 36.0 41.0 31.0 41.0 44-45 36.57213477629691 40.0 36.0 41.0 30.5 41.0 46-47 36.12852716984166 40.0 36.0 41.0 30.0 41.0 48-49 35.731152099478145 39.0 36.0 41.0 28.0 41.0 50-51 34.81919855421482 38.5 35.0 40.0 25.5 40.5 52-53 34.86317734146517 38.5 35.0 40.0 26.0 40.5 54-55 35.19049362390116 39.0 35.0 40.5 26.0 41.0 56-57 35.003921311364394 39.0 35.0 41.0 24.0 41.0 58-59 34.79536172139855 39.0 35.0 41.0 23.0 41.0 60-61 34.51011330113894 39.0 35.0 40.0 22.0 41.0 62-63 34.21697358564034 38.5 34.5 40.0 19.5 41.0 64-65 33.88147820529642 38.0 34.0 40.0 17.5 41.0 66-67 33.52017581357159 38.0 34.0 40.0 13.0 41.0 68-69 33.076627460607014 37.0 33.5 40.0 8.0 41.0 70-71 32.65692652143918 36.5 33.0 39.0 4.5 41.0 72-73 32.25309705298532 36.0 32.5 39.0 2.0 40.5 74-75 31.815531186381854 35.5 32.0 38.5 2.0 40.0 76-77 31.36467984069276 35.0 32.0 37.5 2.0 39.5 78-79 30.88957900394882 35.0 31.0 37.0 2.0 39.0 80-81 30.399451058732907 35.0 31.0 36.5 2.0 39.0 82-83 29.923266729588228 35.0 30.5 36.0 2.0 37.5 84-85 29.466980992326675 34.0 30.0 36.0 2.0 37.0 86-87 29.0494597351369 34.0 29.5 35.0 2.0 37.0 88-89 28.615389824482698 34.0 29.0 35.0 2.0 36.0 90-91 28.206930118972544 34.0 29.0 35.0 2.0 36.0 92-93 27.824201241783868 34.0 29.0 35.0 2.0 35.5 94-95 27.31611948889632 34.0 26.5 35.0 2.0 35.0 96-97 26.742810223766575 34.0 25.5 35.0 2.0 35.0 98-99 26.04589606091259 33.0 24.5 35.0 2.0 35.0 100-101 24.235720966847673 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16409539368935455 1101 2 0.14117037148978895 1101 3 0.36066450387868443 1101 4 0.12232051826646284 1101 5 0.23933576305663706 1101 6 0.33870756990808815 1101 7 0.32562665727355977 1101 8 0.4232781654934854 1101 9 0.4578760858465145 1101 10-11 0.3146790443705143 1101 12-13 0.33370608168888793 1101 14-15 0.39064769512430075 1101 16-17 0.42246945816579284 1101 18-19 0.5275727282546754 1101 20-21 0.44018668754296186 1101 22-23 0.6101668288364621 1101 24-25 0.5655022194539825 1101 26-27 0.44953894304065045 1101 28-29 0.42264368519233386 1101 30-31 0.352625378752208 1101 32-33 0.5087105600451451 1101 34-35 0.6949630477312851 1101 36-37 0.6405284911851581 1101 38-39 0.859681196180567 1101 40-41 0.8007255389933405 1101 42-43 0.8792517297212896 1101 44-45 1.091504232960368 1101 46-47 1.0945483354694048 1101 48-49 1.5623923253311176 1101 50-51 1.3128219934026149 1101 52-53 1.606648306890591 1101 54-55 1.7316897524750061 1101 56-57 1.6341519751784332 1101 58-59 1.9429237572606226 1101 60-61 1.9138026765411382 1101 62-63 1.8937126562952074 1101 64-65 1.7508560581976056 1101 66-67 1.8521420966850997 1101 68-69 1.794910379079063 1101 70-71 1.8318943805994437 1101 72-73 1.5619125678563854 1101 74-75 1.6712887517632247 1101 76-77 2.0129954009662576 1101 78-79 1.9531708661494385 1101 80-81 1.8322063070302086 1101 82-83 2.1528345095798436 1101 84-85 2.131630903313699 1101 86-87 2.0303497796699403 1101 88-89 2.141942902431307 1101 90-91 1.7161683181805216 1101 92-93 1.7151492815098734 1101 94-95 1.4069191139235855 1101 96-97 1.960231214875833 1101 98-99 2.463249218544526 1101 100-101 2.953909493500447 1102 1 -0.16350721721893535 1102 2 0.1252172793335795 1102 3 0.07370798213954899 1102 4 0.19423112784716778 1102 5 0.12627015845435352 1102 6 -0.24261779794233718 1102 7 0.008202163413621122 1102 8 0.037859424482313386 1102 9 0.248428138064682 1102 10-11 0.37053699325866063 1102 12-13 0.457768477971662 1102 14-15 0.500482853880797 1102 16-17 0.397566260909457 1102 18-19 0.3307921109297496 1102 20-21 0.38718303669663356 1102 22-23 0.29449031594787556 1102 24-25 0.5211942207815596 1102 26-27 0.29888068438371107 1102 28-29 0.41508753960100364 1102 30-31 0.4672508904238555 1102 32-33 0.508334412241183 1102 34-35 0.4024749406397845 1102 36-37 0.13178858632843315 1102 38-39 0.2415265330541203 1102 40-41 0.26084280860280984 1102 42-43 0.34561969077892485 1102 44-45 0.12112034093691193 1102 46-47 0.5334353804965133 1102 48-49 0.7559314336395602 1102 50-51 0.6916138951616588 1102 52-53 0.807854192497409 1102 54-55 0.8819829264986794 1102 56-57 1.1082752189000828 1102 58-59 0.8395494901956795 1102 60-61 1.0376769989219383 1102 62-63 1.0457648744609287 1102 64-65 0.982087389097039 1102 66-67 0.8281350162558496 1102 68-69 0.7674405262192394 1102 70-71 1.3774857070736104 1102 72-73 1.3994056533746964 1102 74-75 1.5976522875525738 1102 76-77 1.3444590584505 1102 78-79 1.3200919857423123 1102 80-81 1.392976303711258 1102 82-83 1.2133611165053857 1102 84-85 1.3625856549455158 1102 86-87 1.5464245667039052 1102 88-89 1.5090963486089777 1102 90-91 1.2416136108013873 1102 92-93 1.5167313149206514 1102 94-95 1.4081803153839267 1102 96-97 1.5095618930482004 1102 98-99 1.5809281653306755 1102 100-101 2.3127987511616936 1103 1 -0.03389241935471077 1103 2 -0.10491239267252439 1103 3 -0.29503725050728136 1103 4 -0.3331334793229317 1103 5 -0.17278590561887341 1103 6 -0.09047248901785565 1103 7 -0.19113040714320562 1103 8 0.11233501563410186 1103 9 -0.06193608771716441 1103 10-11 -0.10544031414026733 1103 12-13 -0.06457133896198286 1103 14-15 -0.05559075405207636 1103 16-17 -0.026280039624488438 1103 18-19 0.146977274926698 1103 20-21 -0.434332028139373 1103 22-23 -0.20588630495220883 1103 24-25 -0.15221825061431815 1103 26-27 0.051035528014530485 1103 28-29 0.02190962198086055 1103 30-31 -0.07267092498423011 1103 32-33 -0.06259903550862589 1103 34-35 -0.3228444720223038 1103 36-37 0.4325132240095897 1103 38-39 0.33830380231422197 1103 40-41 0.2282817864822917 1103 42-43 0.28929341312828427 1103 44-45 0.3221691586333293 1103 46-47 0.6544117344248122 1103 48-49 0.44654928260980853 1103 50-51 0.20264555038666998 1103 52-53 0.6429371444424916 1103 54-55 0.4941110729517675 1103 56-57 0.1172902837360894 1103 58-59 0.20018068775479492 1103 60-61 0.44505686162216307 1103 62-63 0.37759172419395526 1103 64-65 0.38328876209475027 1103 66-67 0.41010965393700616 1103 68-69 0.5915824026494434 1103 70-71 0.6198865461277734 1103 72-73 0.691005577097048 1103 74-75 0.8562525926631785 1103 76-77 0.7616310645527129 1103 78-79 0.6158156699528412 1103 80-81 0.7150491488676245 1103 82-83 0.22437846967090636 1103 84-85 0.2940120470126395 1103 86-87 0.40055786189841314 1103 88-89 0.35938715715207437 1103 90-91 0.843039049397877 1103 92-93 0.17910736373834268 1103 94-95 0.15936738632214187 1103 96-97 0.48011871989793065 1103 98-99 0.8592081043085535 1103 100-101 1.0059719014286657 1104 1 0.05127340616800069 1104 2 0.29378984236712924 1104 3 0.3560982938330177 1104 4 0.2806913224074279 1104 5 0.11078475047341385 1104 6 0.11658410795402574 1104 7 0.1407910059786346 1104 8 0.4317744532692842 1104 9 0.2366850370124638 1104 10-11 0.27093405500176715 1104 12-13 0.32985007415986445 1104 14-15 0.5749517837994063 1104 16-17 0.3867066222356357 1104 18-19 0.6044619540903611 1104 20-21 0.37580225448500215 1104 22-23 0.3907926784652389 1104 24-25 0.24483924758322928 1104 26-27 0.5663563643519467 1104 28-29 0.3118564496665144 1104 30-31 0.5622876186350965 1104 32-33 0.6235716566326914 1104 34-35 0.9042418653965854 1104 36-37 0.7321707582561672 1104 38-39 0.5525251433380163 1104 40-41 0.3329591476935363 1104 42-43 0.23456648450535056 1104 44-45 0.34424918747254196 1104 46-47 0.5103958728530671 1104 48-49 0.33200350034593384 1104 50-51 0.31813761960081166 1104 52-53 0.5270620543199271 1104 54-55 0.7380728609834932 1104 56-57 0.7189710696048692 1104 58-59 0.6408349775889732 1104 60-61 0.8052767606848903 1104 62-63 0.5999240962199153 1104 64-65 0.6269801824264007 1104 66-67 0.8377637635260484 1104 68-69 1.212526887148364 1104 70-71 1.390479552616597 1104 72-73 1.428703846743769 1104 74-75 1.044158144213295 1104 76-77 0.9147042440768622 1104 78-79 1.0057084240984757 1104 80-81 1.2851194444696468 1104 82-83 1.5615495223024887 1104 84-85 1.796986091713432 1104 86-87 1.6812220652149215 1104 88-89 1.6732615262941088 1104 90-91 1.6588510537237617 1104 92-93 1.5595147177397877 1104 94-95 1.545970666783564 1104 96-97 1.7228187919959836 1104 98-99 1.619572199477986 1104 100-101 2.054187474608188 1105 1 0.31108609836816825 1105 2 0.19809512612446767 1105 3 0.18243321136167268 1105 4 0.3440684540820129 1105 5 0.2824369730062628 1105 6 0.21842922956431465 1105 7 0.35626486675582214 1105 8 0.23726788351810058 1105 9 0.06753491120871757 1105 10-11 0.1573752068049501 1105 12-13 0.008079036977875376 1105 14-15 0.1273979677524366 1105 16-17 0.3294798959286851 1105 18-19 0.14478205473336203 1105 20-21 0.01430518990573404 1105 22-23 -0.05602728239581012 1105 24-25 0.13894044970790986 1105 26-27 0.44268429278359633 1105 28-29 0.12538035237829348 1105 30-31 -0.1325124931904611 1105 32-33 0.14075085707999335 1105 34-35 0.2219662154105677 1105 36-37 -0.1011602453781606 1105 38-39 0.5024552523679304 1105 40-41 0.883853752110646 1105 42-43 1.0305058191433218 1105 44-45 0.926385055518395 1105 46-47 1.3060766109017123 1105 48-49 1.109170120418618 1105 50-51 1.1205698253598229 1105 52-53 0.8208976707582778 1105 54-55 1.138149741050583 1105 56-57 0.985234698344037 1105 58-59 1.2804841249191412 1105 60-61 1.4259644878305409 1105 62-63 1.0668237327868653 1105 64-65 1.3441104591946456 1105 66-67 1.3053902661819023 1105 68-69 1.5295523274614737 1105 70-71 1.4082458372126254 1105 72-73 1.2173884986867414 1105 74-75 1.686945396100345 1105 76-77 1.4237551247030886 1105 78-79 1.5180511694157808 1105 80-81 1.6969100511232647 1105 82-83 1.518182175363716 1105 84-85 1.3588885239191946 1105 86-87 0.9785452410606048 1105 88-89 0.7678659434034181 1105 90-91 0.9178040869918611 1105 92-93 1.1245692326964551 1105 94-95 1.3807623916927092 1105 96-97 1.632750298845302 1105 98-99 1.8879675028047949 1105 100-101 1.5407194846724899 1106 1 0.05445344530694385 1106 2 0.25434512612446625 1106 3 0.04965259911677933 1106 4 -0.38858460714247656 1106 5 -0.18758853719781854 1106 6 -0.08718301533364325 1106 7 -0.13098003120335733 1106 8 -0.013369871583940096 1106 9 0.001973686718926615 1106 10-11 -0.5570380585011705 1106 12-13 -0.5870102487364193 1106 14-15 -0.21054846081899115 1106 16-17 -0.22964000203050716 1106 18-19 -0.1644653942462284 1106 20-21 -0.5129269529513962 1106 22-23 -0.12611656811009908 1106 24-25 -0.09148046865943371 1106 26-27 -0.29870601333885816 1106 28-29 0.09451300543950936 1106 30-31 0.2467604659932192 1106 32-33 0.33730697952897515 1106 34-35 0.10079274602281174 1106 36-37 0.2338525097238815 1106 38-39 -0.21667740069329255 1106 40-41 0.010001711294322035 1106 42-43 0.22514867628617452 1106 44-45 0.437418218783705 1106 46-47 1.0352857945751879 1106 48-49 1.045585936745148 1106 50-51 1.1029040090332956 1106 52-53 1.300361956472571 1106 54-55 1.3114278022750767 1106 56-57 0.6222244942624044 1106 58-59 0.7402417779803585 1106 60-61 0.9981583653815633 1106 62-63 0.8730099572766647 1106 64-65 1.334416581643616 1106 66-67 1.6891275110798603 1106 68-69 0.9893737560329043 1106 70-71 1.4342662453758948 1106 72-73 1.5974905395030596 1106 74-75 1.2012948858962638 1106 76-77 1.4907194104173733 1106 78-79 1.636609842885175 1106 80-81 1.952713622551837 1106 82-83 2.063781665159631 1106 84-85 1.7149471973885824 1106 86-87 1.391746771672846 1106 88-89 1.7024322699340324 1106 90-91 1.4566433727061465 1106 92-93 1.790831987798498 1106 94-95 1.9201756569988326 1106 96-97 2.264893155988158 1106 98-99 2.1285925028047963 1106 100-101 1.8587679540602444 1107 1 0.12985027070376987 1107 2 -0.1168519638226222 1107 3 0.04122005943423446 1107 4 -0.04731476587263472 1107 5 -0.11930150016078045 1107 6 0.0192984661478377 1107 7 -0.0873292375525665 1107 8 -0.07272833719240879 1107 9 -0.027457530212295467 1107 10-11 -0.05323514844825894 1107 12-13 -0.21077016937133664 1107 14-15 -0.18582954547507313 1107 16-17 0.07715563289013261 1107 18-19 -0.03144621435205153 1107 20-21 -0.22398846088790947 1107 22-23 -0.12785267922121335 1107 24-25 0.04021595991199689 1107 26-27 0.305543325285484 1107 28-29 -0.3066113331848257 1107 30-31 0.018337846945598812 1107 32-33 0.10169190016389251 1107 34-35 0.1745361322661907 1107 36-37 0.40242063141699447 1107 38-39 0.43345487443898634 1107 40-41 0.51909562663824 1107 42-43 0.5121857133232126 1107 44-45 0.5372032716937554 1107 46-47 0.9504643660037573 1107 48-49 0.5208670214012301 1107 50-51 0.785857051361333 1107 52-53 0.5412680411286459 1107 54-55 0.5785408975131787 1107 56-57 0.6222244942624044 1107 58-59 0.7414818573454482 1107 60-61 1.0940578362810385 1107 62-63 1.2568558567475634 1107 64-65 0.9071679043949459 1107 66-67 1.5038596539370062 1107 68-69 1.3823962428053918 1107 70-71 1.1419382030478502 1107 72-73 1.0019217564342817 1107 74-75 1.06166194938832 1107 76-77 1.0785170294649866 1107 78-79 1.3876845783349054 1107 80-81 1.5377830669962798 1107 82-83 1.3786791519321184 1107 84-85 1.2471065889229713 1107 86-87 1.3534696552707324 1107 88-89 1.0551108413626018 1107 90-91 0.8943913885791623 1107 92-93 1.316046934888444 1107 94-95 1.110817191390364 1107 96-97 0.6762688173638196 1107 98-99 0.7134966033338976 1107 100-101 0.7632818429491302 1108 1 -0.1820526956109596 1108 2 -0.18600312856202095 1108 3 -0.3210851190474102 1108 4 0.12563646590212585 1108 5 0.20791485646734031 1108 6 -0.20539568501690297 1108 7 -0.5669044009512589 1108 8 -0.17200351734994257 1108 9 -0.26503503984862675 1108 10-11 -0.42809898287091386 1108 12-13 -0.28112587575645875 1108 14-15 -0.34487093981059047 1108 16-17 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-2.4647443109028018 2316 94-95 -2.925100317027141 2316 96-97 -2.9290678829728805 2316 98-99 -3.4117727569354663 2316 100-101 -3.1428797732124814 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 778.0 3 113.0 4 94.0 5 91.0 6 116.0 7 141.0 8 179.0 9 242.0 10 327.0 11 457.0 12 755.0 13 1049.0 14 1235.0 15 1804.0 16 2615.0 17 3393.0 18 2939.0 19 1706.0 20 1657.0 21 1612.0 22 1579.0 23 1696.0 24 1816.0 25 1931.0 26 2190.0 27 2454.0 28 2874.0 29 3634.0 30 4428.0 31 5494.0 32 7186.0 33 9480.0 34 13193.0 35 18907.0 36 29065.0 37 47345.0 38 52522.0 39 9175.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.88613897106573 21.896580264258095 15.288430113368676 20.928850651307496 2 14.742072078952503 23.09555934590939 40.18906511205587 21.973303463082235 3 19.027153160797624 26.254560882477723 31.74798472634705 22.9703012303776 4 11.731734534977956 19.316267603542137 39.6334814173636 29.31851644411631 5 11.67991921180594 39.72750956813958 35.57777985217118 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29.65385813885951 32.09311001965053 18.843734830193558 28-29 18.566046696022166 30.966378270428187 31.638431254491838 18.829143779057812 30-31 19.58084809984331 30.142975579864256 31.435120298376447 18.841056021915996 32-33 19.099563244710172 30.163041173726835 31.56998685493788 19.167408726625112 34-35 19.76574311043448 30.350346360091514 31.11876323897044 18.765147290503563 36-37 19.47166803402931 29.990608656571503 31.499371437172336 19.03835187222685 38-39 19.13211603162186 30.685882310541256 31.356437049898346 18.825564607938535 40-41 19.690327394832572 30.539679982362568 30.921259041303813 18.848733581501047 42-43 19.273054907780367 30.73818104727581 30.407886368454527 19.580877676489294 44-45 19.77120614978874 30.530156394758436 30.26388037931612 19.43475707613671 46-47 19.936991981954097 30.86226854305619 29.85609070440929 19.344648770580427 48-49 19.81769955410812 30.473942994536074 30.19406792059181 19.514289530763996 50-51 19.36795769421603 30.803580894545114 30.186338827939796 19.642122583299056 52-53 19.74455975469594 31.20862296375149 29.36799426605991 19.678823015492668 54-55 19.604588050714497 30.30763685705805 29.61497689013272 20.47279820209473 56-57 19.97986114791457 30.591764269436645 29.335735863055806 20.092638719592983 58-59 20.40779119462723 30.33397496777695 29.23817922800353 20.020054609592293 60-61 19.711741275181303 30.023594055061274 30.267802064320826 19.996862605436597 62-63 19.586601653593387 29.76807292770829 30.319482722069115 20.325842696629213 64-65 19.469829459336278 30.066244819655104 30.097406382820864 20.366519338187754 66-67 19.53659932588557 29.601466940834797 29.838890890975772 21.02304284230386 68-69 19.41290689096721 29.230224611445095 30.31957740149402 21.03729109609368 70-71 19.722830545880086 29.163212825182267 30.12781510164364 20.986141527294006 72-73 19.10997483660754 28.853528704710662 30.881651498673996 21.1548449600078 74-75 18.987038536715666 29.75539848973725 30.23472247544021 21.02284049810687 76-77 19.239044947988543 29.168177086976893 30.131666253288476 21.46111171174609 78-79 19.02212047017923 29.250532608350028 30.06539688596352 21.661950035507225 80-81 19.114417271555524 29.113314904009226 29.903839630961265 21.868428193473985 82-83 19.11794010777717 28.935329458103855 30.304591341363622 21.642139092755354 84-85 19.2208227169245 28.544114093362218 30.100645675500363 22.134417514212917 86-87 19.329592595968567 28.26178660838635 29.84400404457512 22.56461675106997 88-89 19.45765140547266 27.555361484474194 29.822601602923505 23.16438550712964 90-91 19.20388011989706 27.078166270260184 30.600460425066455 23.117493184776293 92-93 19.347781847781846 27.530609822276492 30.73976563559897 22.381842694342694 94-95 19.088974338320398 28.45868821462033 30.026878415445342 22.425459031613933 96-97 18.87486354434941 29.596727184085296 29.064682628003347 22.463726643561944 98-99 19.807355182522908 29.560211044432528 27.995395856694984 22.637037916349584 100-101 20.053628963879937 28.84962692894692 28.29129218246566 22.80545192470748 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 379.0 1 218.0 2 65.5 3 84.0 4 80.5 5 67.5 6 94.5 7 159.5 8 217.5 9 260.0 10 233.5 11 198.5 12 213.5 13 223.5 14 243.5 15 289.0 16 340.0 17 401.5 18 479.0 19 554.0 20 646.0 21 770.5 22 914.0 23 1083.5 24 1339.5 25 1679.0 26 2150.0 27 2817.0 28 3478.0 29 4172.0 30 4895.0 31 5625.5 32 6405.0 33 7105.0 34 7896.0 35 8765.0 36 9434.0 37 9781.0 38 9637.5 39 9388.0 40 9360.0 41 9248.5 42 9038.0 43 8977.0 44 8801.5 45 8494.5 46 8682.5 47 9644.5 48 11220.5 49 10392.0 50 7745.0 51 6181.0 52 5065.5 53 4224.0 54 3639.0 55 3110.0 56 2484.5 57 1911.0 58 1533.0 59 1200.5 60 888.5 61 607.0 62 388.5 63 266.0 64 172.0 65 120.0 66 79.0 67 52.5 68 38.0 69 32.0 70 23.0 71 13.0 72 7.0 73 3.5 74 4.0 75 5.5 76 6.0 77 5.0 78 2.5 79 0.5 80 0.0 81 0.5 82 0.5 83 1.0 84 1.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.5 95 1.5 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28357027675612534 2 0.2484414215758889 3 0.242516072509343 4 0.25225057454723987 5 0.25182733532820084 6 0.1934203231008198 7 0.17776047199637707 8 0.18580201715811792 9 0.182839342624845 10-11 0.20315482513871666 12-13 0.19278546427226134 14-15 0.18368582106292297 16-17 0.18643687598667644 18-19 0.182416103405806 20-21 0.17818371121541607 22-23 0.17924180926301353 24-25 0.1703537856631947 26-27 0.1699305464441557 28-29 0.1813580053582085 30-31 0.19617137802457324 32-33 0.1874949740342739 34-35 0.19617137802457324 36-37 0.17670237394877958 38-39 0.17924180926301353 40-41 0.17395131902502614 42-43 0.17903018965349404 44-45 0.1809347661391695 46-47 0.181992864186767 48-49 0.19193898583418334 50-51 0.19722947607217078 52-53 0.2048477820148726 54-55 0.1874949740342739 56-57 0.17373969941550665 58-59 0.1764907543392601 60-61 0.17289322097742865 62-63 0.17903018965349404 64-65 0.17162350332031168 66-67 0.17268160136790917 68-69 0.1688724483965582 70-71 0.17966504848205253 72-73 0.1754326562916626 74-75 0.17776047199637707 76-77 0.17416293863454563 78-79 0.17162350332031168 80-81 0.1764907543392601 82-83 0.17606751512022112 84-85 0.16760273073944124 86-87 0.1699305464441557 88-89 0.16569815425376577 90-91 0.1709886444917532 92-93 0.1697189268346362 94-95 0.16760273073944124 96-97 0.1652749150347268 98-99 0.16866082878703872 100-101 0.16633301308232426 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 236273.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.64976562665754 #Duplication Level Percentage of deduplicated Percentage of total 1 90.01622442521028 71.69771178064605 2 7.667454190356684 12.214218584300864 3 1.3514214616815838 3.2292120805731934 4 0.3871135522184748 1.233340148204175 5 0.1356734817620126 0.5403180512048451 6 0.08454330703788424 0.4040312754522016 7 0.06091201127350624 0.33961391952481657 8 0.04737096145644695 0.3018468782012342 9 0.02656190218488234 0.19040843552616732 >10 0.17553440766756123 2.821803802960449 >50 0.023860263615530272 1.2982283511676729 >100 0.020148667053114452 3.303429460908763 >500 0.0015906842410353514 0.7905931923831763 >1k 0.0015906842410353514 1.635244038946399 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1519 0.6429003737202305 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1183 0.5006919961231288 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1170 0.4951898862756219 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 759 0.3212385672505957 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 609 0.25775268439474674 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 504 0.2133125663956525 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 460 0.1946900407579368 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 372 0.1574449894825054 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 370 0.1565985110444274 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 366 0.15490555416827145 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 359 0.1519428796349985 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 318 0.1345900716543998 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 311 0.13162739712112684 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 306 0.1295112010259319 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 298 0.12612528727361993 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 287 0.121469655864191 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 272 0.1151210675786061 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 255 0.10792600085494322 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 246 0.10411684788359228 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 237 0.10030769491224135 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 2.116196095194965E-4 0.0 26-27 0.0 0.0 0.0 0.0019045764856754689 0.0 28-29 0.0 0.0 0.0 0.008676403990299357 0.0 30-31 0.0 0.0 0.0 0.014178513837806266 0.0 32-33 0.0 0.0 0.0 0.023913015875703107 0.0 34-35 0.0 0.0 0.0 0.03258941986600246 0.0 36-37 0.0 0.0 0.0 0.04592145526573074 0.0 38-39 0.0 0.0 0.0 0.0662369377796024 0.0 40-41 0.0 0.0 0.0 0.11342811070245012 0.0 42-43 0.0 0.0 0.0 0.19405518192937832 0.0 44-45 0.0 0.0 0.0 0.2590224020518637 0.0 46-47 0.0 0.0 0.0 0.4264135131817855 0.0 48-49 0.0 0.0 0.0 0.5946511027497852 0.0 50-51 0.0 0.0 0.0 0.7057513977475209 0.0 52-53 0.0 0.0 0.0 0.849864351830298 0.0 54-55 0.0 0.0 0.0 0.9656202782374625 0.0 56-57 0.0 0.0 0.0 1.328971147782438 0.0 58-59 0.0 0.0 0.0 1.7469198765834437 0.0 60-61 0.0 0.0 0.0 2.167619660308203 0.0 62-63 0.0 0.0 0.0 2.654556381812564 0.0 64-65 0.0 0.0 0.0 3.3194651949228224 0.0 66-67 0.0 0.0 0.0 4.246147465008698 0.0 68-69 0.0 0.0 0.0 5.327100430434285 0.0 70-71 0.0 0.0 0.0 6.059515898981263 0.0 72-73 0.0 0.0 0.0 6.653955382121529 0.0 74-75 0.0 0.0 0.0 7.553761961798428 0.0 76-77 0.0 0.0 0.0 8.652702594033173 0.0 78-79 0.0 0.0 0.0 9.626576037041897 0.0 80-81 0.0 0.0 0.0 10.661607547201754 0.0 82-83 0.0 0.0 0.0 11.906565710004951 0.0 84-85 0.0 0.0 0.0 13.933246710373169 0.0 86-87 0.0 0.0 0.0 16.2997888036297 0.0 88-89 0.0 0.0 0.0 18.686223140181063 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGTGC 65 0.0058355336 29.229986 6 GTCTGAG 90 8.9277606E-4 26.38818 1 AATGACG 45 8.941082E-4 26.38818 74-75 GTTATAT 90 8.9277606E-4 26.38818 1 AGGACGT 95 0.0012208265 25.009945 5 ACTGTTG 95 0.0012238531 24.999329 8 GAGTACT 1275 0.0 24.771215 12-13 CCCTACA 100 0.0016500169 23.749365 2 GGATAGA 100 0.0016500169 23.749365 1 GTACTTT 1355 0.0 23.131002 14-15 ATGACGA 75 3.0289424E-5 22.16607 74-75 CGAAATC 65 2.9342613E-4 21.927143 78-79 CTCGGTG 65 2.942697E-4 21.91784 94-95 TAGGACG 110 0.0028640341 21.599497 4 GGACGTG 110 0.0028711017 21.59033 6 GACTGTT 110 0.0028711017 21.59033 7 GTAGGAC 155 3.890291E-5 21.451038 3 GTACATG 895 0.0 20.69777 1 CTAAGCA 115 0.0037152027 20.65162 9 AGTACTT 1335 0.0 20.278204 12-13 >>END_MODULE