FastQCFastQC Report
Thu 26 May 2016
SRR937262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236273
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18530.7842622728792541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15980.6763362720243108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9960.4215462621628371No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9650.40842584637262824No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6900.2920350611369052No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6760.2861097120703593No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5610.2374372018808751No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4890.2069639781100676No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.1692956876155972No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3490.14771048744460857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3360.14220837759710167No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3110.13162739712112684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.1265485264926589No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2830.11977669898803503No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA2780.11766050289284005No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC2700.11427458914052811No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA2650.11215839304533316No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2460.10411684788359228No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC2420.1024238910074363No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGAC250.005018166356.9947974
CATTCCG704.2920856E-640.7191859
TTTAGGC1151.0312942E-733.0404623
TTCTGCG600.00394642131.6637732
GTTAGTC650.005837406529.22813
GTACTGA856.3875056E-427.9445381
TATGGTG856.39542E-427.9386255
ACAGTGC700.00837339827.1461248
ATTGCAC700.00838205127.140383
CCTTTAG1606.1905666E-826.7219641
CTAGGAC908.931199E-426.3864783
GCATTCC1309.967858E-625.5800028
TAGGCAT1501.0549938E-625.331025
TTAGGCA1151.278338E-424.7803464
GCCAGAT500.001651052323.75285712-13
TTGCACT1050.00219226122.6169824
CCCTGGA1503.0222047E-522.1646422
GAGTACT16200.021.84676412-13
AGTACTT16150.021.76732412-13
AGTGGAC1100.00287219921.5889383
Produced by FastQC (version 0.11.5)