##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937262_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 236273 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.135224930482956 33.0 31.0 34.0 31.0 34.0 2 32.34766562408739 34.0 31.0 34.0 31.0 34.0 3 32.42860165994422 34.0 31.0 34.0 31.0 34.0 4 36.068010310107375 37.0 35.0 37.0 35.0 37.0 5 35.9350835685838 37.0 35.0 37.0 35.0 37.0 6 36.01855480736267 37.0 35.0 37.0 35.0 37.0 7 35.98197424166113 37.0 35.0 37.0 35.0 37.0 8 35.96763489692009 37.0 35.0 37.0 35.0 37.0 9 37.657472500031744 39.0 37.0 39.0 35.0 39.0 10-11 37.597361949947725 39.0 37.0 39.0 35.0 39.0 12-13 37.52445899446826 39.0 37.0 39.0 35.0 39.0 14-15 38.726085926026244 40.0 38.0 41.0 35.0 41.0 16-17 38.71275177443043 40.0 38.0 41.0 34.5 41.0 18-19 38.772845394945676 40.0 38.0 41.0 35.0 41.0 20-21 38.72820635451363 40.0 38.0 41.0 34.5 41.0 22-23 38.61394869494187 40.0 38.0 41.0 34.0 41.0 24-25 38.463307275905414 40.0 38.0 41.0 34.0 41.0 26-27 38.25848700443978 40.0 38.0 41.0 34.0 41.0 28-29 38.2273831542326 40.0 38.0 41.0 34.0 41.0 30-31 38.25430116856349 40.0 38.0 41.0 34.0 41.0 32-33 38.130814777820575 40.0 37.0 41.0 34.0 41.0 34-35 37.99206426464302 40.0 37.0 41.0 33.5 41.0 36-37 37.804567174412654 40.0 37.0 41.0 33.0 41.0 38-39 37.61696427437752 40.0 37.0 41.0 33.0 41.0 40-41 37.42598180917837 40.0 37.0 41.0 33.0 41.0 42-43 37.133834589648416 40.0 36.5 41.0 32.5 41.0 44-45 36.71224388736758 40.0 36.0 41.0 31.0 41.0 46-47 36.21349879165203 40.0 36.0 41.0 30.0 41.0 48-49 35.77149102944475 40.0 36.0 41.0 28.5 41.0 50-51 35.51608943891176 39.5 36.0 41.0 27.0 41.0 52-53 35.263290769575875 39.0 35.0 41.0 26.0 41.0 54-55 35.06699876837387 39.0 35.0 41.0 24.5 41.0 56-57 34.81201406847164 39.0 35.0 40.0 23.5 41.0 58-59 34.57639467903654 39.0 35.0 40.0 22.5 41.0 60-61 34.252443148391905 38.5 34.0 40.0 20.5 41.0 62-63 33.93630038133854 38.0 34.0 40.0 18.5 41.0 64-65 33.63488845530382 38.0 34.0 40.0 15.5 41.0 66-67 33.26137984450191 37.5 33.5 40.0 10.5 41.0 68-69 32.83863581534919 37.0 33.0 39.5 7.5 41.0 70-71 32.47317298210122 36.0 32.5 39.0 4.5 40.5 72-73 32.04195358758724 36.0 32.0 39.0 2.0 40.0 74-75 31.628108163014815 35.5 32.0 38.0 2.0 40.0 76-77 30.24150029838365 34.0 30.0 36.5 2.0 39.0 78-79 30.8126489273002 35.0 31.0 37.0 2.0 39.0 80-81 30.621247455274194 35.0 31.0 36.5 2.0 39.0 82-83 30.263157449221875 35.0 31.0 36.0 2.0 37.5 84-85 29.927196505737008 34.5 31.0 36.0 2.0 37.0 86-87 29.568719235799264 34.0 30.5 35.0 2.0 37.0 88-89 29.165850520372622 34.0 30.0 35.0 2.0 36.0 90-91 28.747252119370387 34.0 29.0 35.0 2.0 36.0 92-93 28.417102250362927 34.0 29.0 35.0 2.0 35.5 94-95 28.057664227397968 34.0 29.0 35.0 2.0 35.0 96-97 27.679258738831777 34.0 29.0 35.0 2.0 35.0 98-99 27.1078561663838 34.0 27.0 35.0 2.0 35.0 100-101 25.64933995843791 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08478652583497137 1101 2 0.11712765826452198 1101 3 0.23193018562000134 1101 4 0.0732435632713404 1101 5 -0.1249264124529077 1101 6 -0.190134788891001 1101 7 0.01969058975593896 1101 8 -0.06270921506938265 1101 9 0.00940730399237566 1101 10-11 0.0532212436539794 1101 12-13 0.13588821376603732 1101 14-15 0.2978383862907279 1101 16-17 0.08295315199892173 1101 18-19 0.2814802701295207 1101 20-21 0.09755364597639726 1101 22-23 0.06079560912947812 1101 24-25 0.06277427028063443 1101 26-27 0.12207939595555217 1101 28-29 0.08965071351639153 1101 30-31 0.460834820933961 1101 32-33 0.1875289362029804 1101 34-35 0.17780999726581115 1101 36-37 0.07288220251640354 1101 38-39 0.11093764273213225 1101 40-41 0.3774658210585997 1101 42-43 0.14937162731713016 1101 44-45 0.46293600706425053 1101 46-47 0.43211060624077646 1101 48-49 0.4430448425420579 1101 50-51 0.28702578387280653 1101 52-53 0.35952016247009766 1101 54-55 0.4976969195306751 1101 56-57 0.5332792185708399 1101 58-59 0.4461000159941193 1101 60-61 0.5983466320910154 1101 62-63 0.33261065085164176 1101 64-65 0.4477772792513761 1101 66-67 0.357879739450091 1101 68-69 0.2922597702733398 1101 70-71 0.5260783777852964 1101 72-73 0.2045931912116501 1101 74-75 0.5116280358121443 1101 76-77 0.3523466427646973 1101 78-79 0.4278206042387609 1101 80-81 0.4680995142927493 1101 82-83 0.6544123986652401 1101 84-85 0.6255651933107202 1101 86-87 0.5514436782497114 1101 88-89 0.6119021598473502 1101 90-91 0.9412005510394614 1101 92-93 0.8415871579179637 1101 94-95 0.7182848792208603 1101 96-97 0.6175545280731818 1101 98-99 0.595235669555624 1101 100-101 0.7183661946494801 1102 1 0.21426987576305834 1102 2 0.1302264523789205 1102 3 0.0429491036419023 1102 4 0.12614764558132663 1102 5 -0.028544069273351624 1102 6 -0.03556016780459714 1102 7 -0.03206292280076184 1102 8 0.03221278957715157 1102 9 -0.0039570062189326904 1102 10-11 -0.13286128759947502 1102 12-13 -0.020113556341271988 1102 14-15 0.08995034557825932 1102 16-17 0.043557201119398314 1102 18-19 -0.03109855496175129 1102 20-21 0.2738563343268865 1102 22-23 0.26637144716465855 1102 24-25 0.155350882737757 1102 26-27 0.23378423056115594 1102 28-29 -0.06324230562289301 1102 30-31 -0.12291338130079765 1102 32-33 0.1120006698018301 1102 34-35 0.28461384724921857 1102 36-37 -0.11089918923046582 1102 38-39 0.03253295194775063 1102 40-41 0.022658873387406686 1102 42-43 -1.1393524510339148E-4 1102 44-45 -0.06526401506209112 1102 46-47 -0.0841854442072858 1102 48-49 0.07946480050201643 1102 50-51 -0.05081025993735011 1102 52-53 -0.37403443427732697 1102 54-55 -0.2381698798940377 1102 56-57 0.04703073975681349 1102 58-59 0.20796526657493786 1102 60-61 0.22103719520639942 1102 62-63 0.32582892720964907 1102 64-65 0.57484885796584 1102 66-67 0.5286950951310203 1102 68-69 0.22669942067715 1102 70-71 0.3929773710367641 1102 72-73 0.258846980347613 1102 74-75 -0.22130702337582164 1102 76-77 -0.03800627238078391 1102 78-79 -0.040472348521500834 1102 80-81 0.07340983623101849 1102 82-83 -0.12090566749252218 1102 84-85 -0.13321343264347618 1102 86-87 0.2046243398273191 1102 88-89 0.4323358361389751 1102 90-91 0.21259119159704554 1102 92-93 0.031132461601998784 1102 94-95 0.359473063754546 1102 96-97 0.5716202433071693 1102 98-99 0.4948373954102543 1102 100-101 0.9662918501423441 1103 1 -0.2044276833521188 1103 2 -0.08995852092390777 1103 3 -0.07314548064489657 1103 4 0.11843406815951596 1103 5 -0.16416573212613628 1103 6 -0.009473211282859495 1103 7 -0.12368191593576938 1103 8 0.07302133267402411 1103 9 0.0166474941624557 1103 10-11 -0.19428863313724065 1103 12-13 -0.10085344955255948 1103 14-15 0.19334946838527856 1103 16-17 -0.29342792473872237 1103 18-19 -0.4515743368076741 1103 20-21 -0.04133245285846954 1103 22-23 -0.03958774429224121 1103 24-25 0.014704429648510597 1103 26-27 0.0056044059997475415 1103 28-29 -0.45287807984104944 1103 30-31 -0.131852168486148 1103 32-33 -0.21588357886330556 1103 34-35 -0.050368990279388015 1103 36-37 -0.19336009693450507 1103 38-39 -0.1709233790972533 1103 40-41 -0.027403102203749086 1103 42-43 -0.14791141808263575 1103 44-45 -0.04270966723600367 1103 46-47 0.30965513550285806 1103 48-49 0.37809657014350506 1103 50-51 0.41752498033724805 1103 52-53 0.6100140020308302 1103 54-55 0.5800509439045882 1103 56-57 0.8911287565378956 1103 58-59 0.8035268607778363 1103 60-61 0.8716617184710813 1103 62-63 0.7919902544407691 1103 64-65 0.6928932896973379 1103 66-67 0.631240861721416 1103 68-69 0.662597590013533 1103 70-71 0.6060971269482778 1103 72-73 0.8579554853056663 1103 74-75 0.8410022824975556 1103 76-77 0.38169243089915383 1103 78-79 0.360013921501384 1103 80-81 0.30547982097548854 1103 82-83 0.4934583675951991 1103 84-85 0.19606288314599496 1103 86-87 0.09354977842381018 1103 88-89 0.437622830799544 1103 90-91 0.7296154097511227 1103 92-93 0.46991011225035706 1103 94-95 0.07575357481480438 1103 96-97 0.5162379015832954 1103 98-99 0.8552254579579248 1103 100-101 1.052628615969958 1104 1 0.12672768795285805 1104 2 0.03896181072713745 1104 3 0.12818833334212343 1104 4 -0.03274653746413492 1104 5 -0.26744619355367405 1104 6 -0.0711170468992961 1104 7 0.09914613020698226 1104 8 0.1323219815565011 1104 9 -0.06234493554194387 1104 10-11 -0.12072608807595486 1104 12-13 -0.05777486267440679 1104 14-15 -0.08082591012950502 1104 16-17 0.07972456264411676 1104 18-19 -0.0714598811479803 1104 20-21 0.17969314195047303 1104 22-23 0.057248953616912956 1104 24-25 -0.17225483495133886 1104 26-27 -0.17808791555756898 1104 28-29 -0.12992386210492413 1104 30-31 -0.3889943818963886 1104 32-33 -0.44612420611637305 1104 34-35 -0.8098769210652534 1104 36-37 -0.5719424689604651 1104 38-39 -0.32681288883048154 1104 40-41 -0.535401479997553 1104 42-43 -0.4833926725887565 1104 44-45 -0.347932269975729 1104 46-47 -0.7228791110724799 1104 48-49 -1.0418971212768255 1104 50-51 -0.6773290211047112 1104 52-53 -0.2564694154168947 1104 54-55 -0.21054927238957788 1104 56-57 -0.19155869840081863 1104 58-59 0.17381795666824473 1104 60-61 0.007318711982257753 1104 62-63 -0.14190484289159855 1104 64-65 -0.49323234116062764 1104 66-67 -0.09474147425551394 1104 68-69 -0.27904804805424277 1104 70-71 -0.21888484853838008 1104 72-73 -0.24471664879671806 1104 74-75 -0.09739894316934894 1104 76-77 0.1888301381810571 1104 78-79 0.33320245791090386 1104 80-81 -0.05922367578009968 1104 82-83 -0.23060886383955292 1104 84-85 -0.45924082990375226 1104 86-87 -0.8791656145105797 1104 88-89 -0.5998906875854608 1104 90-91 -0.7866742441782186 1104 92-93 -0.5197708538635553 1104 94-95 -0.40295496880451864 1104 96-97 -0.6573156312213229 1104 98-99 -0.24379671219347898 1104 100-101 -0.4691359838858453 1105 1 0.018410662512543752 1105 2 0.13046306876460534 1105 3 0.05188137220150679 1105 4 -0.16678343989634214 1105 5 -0.1663139598382699 1105 6 0.01501658463551081 1105 7 0.11097168234564947 1105 8 0.16099125748605303 1105 9 -0.05678161432304307 1105 10-11 0.050723450643644696 1105 12-13 0.021649235732070338 1105 14-15 -0.11753264761901505 1105 16-17 0.0675146101699724 1105 18-19 -0.3365703088807166 1105 20-21 -0.27370355919574507 1105 22-23 -0.04628752946947401 1105 24-25 -0.14411673039445105 1105 26-27 -0.17165794630959397 1105 28-29 -0.3088796910118319 1105 30-31 0.22544509252351475 1105 32-33 0.2556859700088694 1105 34-35 0.31602467244886157 1105 36-37 -0.004658432058029405 1105 38-39 -0.08713578511228803 1105 40-41 -0.32546298405122087 1105 42-43 -0.5343641570729645 1105 44-45 -0.37600048356252813 1105 46-47 -0.18575302776245195 1105 48-49 0.3202219729362028 1105 50-51 0.28193153243176994 1105 52-53 0.4395118538031184 1105 54-55 0.2647582478788095 1105 56-57 0.2656051260330585 1105 58-59 0.588858493430898 1105 60-61 0.5497565090188843 1105 62-63 0.5390498677597861 1105 64-65 0.020249626969089718 1105 66-67 -0.0589712757651597 1105 68-69 -0.05191637346659661 1105 70-71 0.1150122719536526 1105 72-73 0.03743319744314988 1105 74-75 0.2766225832494378 1105 76-77 0.05341638363814738 1105 78-79 0.40868470560449666 1105 80-81 0.2619043644771004 1105 82-83 0.11106712162312604 1105 84-85 0.25658248572387166 1105 86-87 0.2910188976504493 1105 88-89 0.13106402306592102 1105 90-91 -0.1704315827300782 1105 92-93 -0.0978530456443778 1105 94-95 -0.06223648963202777 1105 96-97 0.14940788010101613 1105 98-99 0.26221391123397453 1105 100-101 0.4087644913727466 1106 1 -0.11819648034459718 1106 2 0.11375388509113549 1106 3 0.21374361709946044 1106 4 0.097757376430188 1106 5 0.0409564483249909 1106 6 0.04409821728857111 1106 7 0.21160943744769156 1106 8 -0.006865885371091451 1106 9 0.10546328363614066 1106 10-11 0.1332489608477303 1106 12-13 -0.060557396920984274 1106 14-15 0.1737301074830242 1106 16-17 0.22102226323119822 1106 18-19 0.4001643849968346 1106 20-21 0.23662807345731807 1106 22-23 0.18961808277542502 1106 24-25 0.21360010634024462 1106 26-27 0.14434970675162617 1106 28-29 0.30910500286572073 1106 30-31 0.1639655006867784 1106 32-33 0.2177395414374459 1106 34-35 0.42131803979579985 1106 36-37 0.12818595569707014 1106 38-39 0.26822135774485645 1106 40-41 0.19698599554060792 1106 42-43 0.23495982251886716 1106 44-45 0.7407724756211351 1106 46-47 0.5194765640742887 1106 48-49 1.0480918708953837 1106 50-51 1.2844187773297335 1106 52-53 1.01215215992557 1106 54-55 1.3328704927767703 1106 56-57 1.1271484933799982 1106 58-59 1.1160268607778363 1106 60-61 1.1443355906515364 1106 62-63 0.9088840514332546 1106 64-65 1.1252241167650041 1106 66-67 1.4411562752552456 1106 68-69 1.1168974020436053 1106 70-71 1.4648847209332487 1106 72-73 1.4366678913206954 1106 74-75 1.6605511546780107 1106 76-77 1.1513117918014082 1106 78-79 1.3732892974412358 1106 80-81 1.3443660991709727 1106 82-83 1.1890645706027136 1106 84-85 1.4810722816422377 1106 86-87 1.6111719588749374 1106 88-89 1.2656303495965346 1106 90-91 1.262157702984208 1106 92-93 1.4494683829270514 1106 94-95 1.5465007552659316 1106 96-97 1.88371910459081 1106 98-99 2.2000327887849913 1106 100-101 2.113483879127852 1107 1 -0.3366157925139106 1107 2 -0.15757547998823185 1107 3 -0.29634236173651374 1107 4 -0.25839341722060283 1107 5 -0.49823005961151523 1107 6 -0.19928802609767615 1107 7 -0.3236088165205686 1107 8 -0.18940556791077512 1107 9 -0.2926848645120046 1107 10-11 -0.2759772296284666 1107 12-13 -0.3967842487728319 1107 14-15 -0.3109755803476659 1107 16-17 -0.5077310436471123 1107 18-19 -0.3213964615640137 1107 20-21 -0.20665896357972002 1107 22-23 -0.305669615637278 1107 24-25 -0.25539790953276764 1107 26-27 -0.47130836203143645 1107 28-29 -0.7443010817903613 1107 30-31 -0.187224975503689 1107 32-33 -0.3124357231128201 1107 34-35 -0.14771304486028214 1107 36-37 -0.6571149702288537 1107 38-39 -0.4424267904032959 1107 40-41 -0.6244425758879615 1107 42-43 -0.5146268176927649 1107 44-45 -0.16886707464340844 1107 46-47 -0.5977522719045467 1107 48-49 -0.573353234924717 1107 50-51 -0.34380542901947564 1107 52-53 -0.2546302474818347 1107 54-55 -0.11951045960418583 1107 56-57 -0.2565320621755589 1107 58-59 -0.24624165774068274 1107 60-61 -0.42469549400454554 1107 62-63 -0.2239697845455808 1107 64-65 -0.2780496927588061 1107 66-67 0.0306073334563024 1107 68-69 -0.19022892070771746 1107 70-71 -0.4651748028762803 1107 72-73 -0.5348929552401458 1107 74-75 -0.588456781829926 1107 76-77 -0.11513992777530646 1107 78-79 -0.2676663903894543 1107 80-81 -0.10247583204595756 1107 82-83 0.3439091473222895 1107 84-85 -0.008676395606439513 1107 86-87 -0.27407936387638543 1107 88-89 -0.20278896257277879 1107 90-91 -0.03141041870891215 1107 92-93 -0.012667860459195879 1107 94-95 0.175717025107204 1107 96-97 0.031040533162240536 1107 98-99 -0.0338461794689735 1107 100-101 0.05677091616488994 1108 1 -0.20899722371887108 1108 2 -0.056959592607640275 1108 3 -0.2169892529716435 1108 4 -0.2578906520119588 1108 5 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48-49 -0.18675390832539307 2316 50-51 -0.1542094694235132 2316 52-53 -0.1572228400744322 2316 54-55 -0.05216197475570539 2316 56-57 -0.009385597529096401 2316 58-59 -0.0534049574039841 2316 60-61 0.24203851272945798 2316 62-63 -0.1242896498654531 2316 64-65 -0.2904739351830443 2316 66-67 -0.3128859325369646 2316 68-69 -0.17069188367067767 2316 70-71 -0.40146268166415666 2316 72-73 -0.4432347060818955 2316 74-75 0.0264927131195698 2316 76-77 -0.27971905235443373 2316 78-79 -0.505452585675652 2316 80-81 -0.42703812160824484 2316 82-83 -0.19423900082585632 2316 84-85 -0.333213432643479 2316 86-87 -0.4965877813848003 2316 88-89 -0.30136640365021705 2316 90-91 -0.524457556756051 2316 92-93 -0.912398500189834 2316 94-95 -0.5700658031756234 2316 96-97 -0.7198685577468495 2316 98-99 -0.8765987047214985 2316 100-101 -0.6304933935994192 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 1.0 5 6.0 6 9.0 7 25.0 8 54.0 9 128.0 10 239.0 11 446.0 12 733.0 13 1009.0 14 1387.0 15 2056.0 16 2927.0 17 3950.0 18 3474.0 19 1949.0 20 1436.0 21 1319.0 22 1354.0 23 1398.0 24 1516.0 25 1625.0 26 1773.0 27 2220.0 28 2782.0 29 3468.0 30 4217.0 31 5496.0 32 6954.0 33 9082.0 34 12581.0 35 18048.0 36 28310.0 37 49037.0 38 57237.0 39 8025.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.21022209989202 21.513836251614407 16.3252948275497 20.95064682094387 2 15.044461279960048 22.69281720721369 40.29322013095021 21.96950138187605 3 18.876892408358128 25.542063629784188 32.899654213558044 22.681389748299637 4 11.951005828004046 18.996245868127122 40.06890334485955 28.983844959009282 5 11.63779967492889 39.03604564540159 36.20691114723012 13.119243532439388 6 28.066685571351783 39.355745260778846 18.33937860017861 14.238190567690765 7 24.60162608507955 35.44840079061086 22.336449784782857 17.613523339526736 8 23.879156738179987 35.16906290604512 21.78454584315601 19.167234512618876 9 24.15045307758398 17.41290794970225 23.199434552403364 35.237204420310405 10-11 22.64689575194796 27.71137624696855 29.69086607441392 19.95086192666957 12-13 24.45155992736846 26.142698117759593 29.520530261024884 19.88521169384706 14-15 20.971503303382104 27.93886732720201 29.674782984090438 21.41484638532545 16-17 18.73701184646574 30.803985220486474 30.61098813660469 19.8480147964431 18-19 18.324141988293203 29.381266585686895 33.728144984826876 18.566446441193026 20-21 19.59893851603865 27.718782933301732 33.75713687133104 18.925141679328572 22-23 19.106823154963198 28.04049621406109 33.4762495291384 19.376431101837312 24-25 19.502904485287115 27.979345882402733 33.71319133627485 18.8045582960353 26-27 19.042570301521998 27.898403507880882 34.3468010902873 18.71222510030982 28-29 18.589271505239815 28.930705808657965 33.78938324275823 18.690639443343997 30-31 19.243499018080858 28.15716631678743 33.795371436310695 18.80396322882102 32-33 18.882606137815156 28.191964380187322 33.79967241284446 19.125757069153057 34-35 19.471755130717433 28.45479593521054 33.2659677576363 18.807481176435733 36-37 19.357057302357866 28.009336657172 33.7002111963703 18.933394844099833 38-39 18.985916685183422 28.64848850374038 33.64589615803786 18.719698653038336 40-41 19.373986871119424 28.603564520702747 33.25305896145561 18.769389646722225 42-43 19.009140760086265 28.731701019473054 32.781021758776205 19.47813646166447 44-45 19.63703004575216 28.694772572405647 32.20829294925785 19.45990443258434 46-47 19.94747601291726 28.721436643205106 31.95604237471061 19.375044969167025 48-49 19.784740533196768 28.634037744473556 31.937631468682415 19.643590253647265 50-51 19.36784990244336 29.27799621624138 31.807485408827922 19.546668472487337 52-53 19.77923842334926 29.54971579486442 30.738383141535426 19.932662640250896 54-55 19.686125795160685 28.61308740313112 31.260237098610506 20.440549703097687 56-57 20.184701595188617 29.03526852412252 30.571626889234064 20.2084029914548 58-59 20.459595467954443 28.887769656287432 30.526128673187376 20.126506202570756 60-61 19.811827843215262 28.478708951086247 31.48688170040589 20.222581505292606 62-63 19.748341960359415 28.45141002145823 31.339171212961276 20.46107680522108 64-65 19.332932666872644 28.686519407634385 31.319490589275965 20.661057336217002 66-67 19.406787910594947 27.991983849191403 31.12416568968947 21.47706255052418 68-69 19.327007317806096 27.99283032762948 31.388266962369798 21.291895392194622 70-71 19.71681063854101 27.822053302747246 31.25071421618213 21.210421842529616 72-73 19.084914484515796 27.350353193128292 31.92260647640653 21.64212584594939 74-75 18.840282215911255 28.410779056430485 31.059621708786022 21.689317018872238 76-77 18.701459752066466 27.738886796206085 31.341075789446954 22.2185776622805 78-79 18.528566531089037 27.774650510214876 31.305100455828637 22.391682502867447 80-81 18.59501508847816 27.54737104959094 30.830649291285926 23.026964570644974 82-83 18.25409589754225 27.528536904343703 31.316104675523654 22.901262522590393 84-85 18.217485705095378 26.947217836993648 31.203946282478316 23.631350175432654 86-87 18.13728187308749 26.728403160750485 31.088825214899714 24.045489751262313 88-89 18.462541212918953 26.363994193157914 30.61119975621421 24.562264837708923 90-91 17.830658268996345 25.78558851415414 31.352007991331437 25.031745225518083 92-93 17.273852730485572 25.794068164262963 31.872559704572147 25.059519400679314 94-95 16.612774206109034 26.794428478920572 31.301926161685845 25.29087115328455 96-97 16.782069893724632 27.532346057315056 30.68526661954603 25.00031742941428 98-99 17.02342885319033 27.77031216603144 29.25887285016835 25.94738613060988 100-101 15.9844062832012 26.500429631448174 29.911237719524742 27.603926365825888 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 168.0 1 116.5 2 57.0 3 67.0 4 67.5 5 54.0 6 79.5 7 141.0 8 200.0 9 249.5 10 235.5 11 203.5 12 217.5 13 232.0 14 249.0 15 268.5 16 303.5 17 365.0 18 443.0 19 533.0 20 641.5 21 779.5 22 930.0 23 1104.5 24 1346.0 25 1629.5 26 2115.5 27 2770.0 28 3323.0 29 4014.0 30 4740.0 31 5442.5 32 6303.0 33 6994.0 34 7707.5 35 8638.0 36 9387.0 37 9745.5 38 9586.0 39 9405.0 40 9501.0 41 9328.5 42 9068.5 43 8898.5 44 8772.5 45 8751.5 46 8733.0 47 9627.5 48 11467.0 49 10516.0 50 7813.5 51 6374.5 52 5339.0 53 4460.0 54 3734.0 55 3199.0 56 2567.0 57 1972.5 58 1551.5 59 1180.0 60 859.5 61 600.0 62 406.0 63 270.0 64 172.5 65 108.0 66 70.0 67 52.0 68 34.5 69 23.5 70 17.5 71 14.0 72 6.0 73 2.5 74 3.5 75 4.0 76 3.0 77 1.5 78 0.5 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04994222784660118 2 0.0 3 0.0 4 0.0 5 0.007195066723662881 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005078870628467916 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001692956876155972 24-25 2.116196095194965E-4 26-27 0.002116196095194965 28-29 0.002116196095194965 30-31 4.23239219038993E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.116196095194965E-4 40-41 0.0 42-43 0.009946121647416336 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.007195066723662881 92-93 0.0023278157047144617 94-95 0.0 96-97 0.0 98-99 0.0019045764856754689 100-101 0.010157741256935832 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 236273.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.07930321598934 #Duplication Level Percentage of deduplicated Percentage of total 1 89.33254273145019 69.75022690984225 2 7.982535172000094 12.4654156825379 3 1.5544937079632268 3.6412135671422514 4 0.477588937173125 1.4915924575257007 5 0.18644065518520941 0.7278578223996841 6 0.09132921366908732 0.4278552819927929 7 0.05845833342231077 0.3195070158547405 8 0.03484711290560876 0.2176670635807074 9 0.034245913719658634 0.24065073731032527 >10 0.19921523847569503 3.0483825705555767 >50 0.024965587254468347 1.3428952038453437 >100 0.019538285677410012 3.2167615943168206 >500 0.0027136507885291684 1.6475785902652875 >1k 0.0010854603154116674 1.4623955028306346 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1853 0.7842622728792541 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1598 0.6763362720243108 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 996 0.4215462621628371 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 965 0.40842584637262824 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 690 0.2920350611369052 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 676 0.2861097120703593 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 561 0.2374372018808751 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 489 0.2069639781100676 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 400 0.1692956876155972 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 349 0.14771048744460857 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 336 0.14220837759710167 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 311 0.13162739712112684 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 299 0.1265485264926589 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 283 0.11977669898803503 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 278 0.11766050289284005 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 270 0.11427458914052811 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 265 0.11215839304533316 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 246 0.10411684788359228 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 242 0.1024238910074363 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 2.116196095194965E-4 0.0 26-27 0.0 0.0 0.0 0.0019045764856754689 0.0 28-29 0.0 0.0 0.0 0.008888023599818853 0.0 30-31 0.0 0.0 0.0 0.01460175305684526 0.0 32-33 0.0 0.0 0.0 0.024336255094742097 0.0 34-35 0.0 0.0 0.0 0.03343589830408045 0.0 36-37 0.0 0.0 0.0 0.04761441214188672 0.0 38-39 0.0 0.0 0.0 0.06877637309383636 0.0 40-41 0.0 0.0 0.0 0.11617916562620359 0.0 42-43 0.0 0.0 0.0 0.19574813880553427 0.0 44-45 0.0 0.0 0.0 0.2607153589280197 0.0 46-47 0.0 0.0 0.0 0.4312807642007339 0.0 48-49 0.0 0.0 0.0 0.6007880714258507 0.0 50-51 0.0 0.0 0.0 0.7140045625187812 0.0 52-53 0.0 0.0 0.0 0.8606569519157923 0.0 54-55 0.0 0.0 0.0 0.9749315410563204 0.0 56-57 0.0 0.0 0.0 1.3384940302108155 0.0 58-59 0.0 0.0 0.0 1.7553846609642236 0.0 60-61 0.0 0.0 0.0 2.176084444688983 0.0 62-63 0.0 0.0 0.0 2.6651373622885393 0.0 64-65 0.0 0.0 0.0 3.3366063832939012 0.0 66-67 0.0 0.0 0.0 4.27429287307479 0.0 68-69 0.0 0.0 0.0 5.358420132643172 0.0 70-71 0.0 0.0 0.0 6.095067993380539 0.0 72-73 0.0 0.0 0.0 6.69522120597783 0.0 74-75 0.0 0.0 0.0 7.602011232768874 0.0 76-77 0.0 0.0 0.0 8.713649041574788 0.0 78-79 0.0 0.0 0.0 9.70021966115468 0.0 80-81 0.0 0.0 0.0 10.74731348905715 0.0 82-83 0.0 0.0 0.0 11.997773761707855 0.0 84-85 0.0 0.0 0.0 14.045616723028022 0.0 86-87 0.0 0.0 0.0 16.441150702788722 0.0 88-89 0.0 0.0 0.0 18.851921294434828 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGCGAC 25 0.0050181663 56.994797 4 CATTCCG 70 4.2920856E-6 40.719185 9 TTTAGGC 115 1.0312942E-7 33.040462 3 TTCTGCG 60 0.003946421 31.663773 2 GTTAGTC 65 0.0058374065 29.2281 3 GTACTGA 85 6.3875056E-4 27.944538 1 TATGGTG 85 6.39542E-4 27.938625 5 ACAGTGC 70 0.008373398 27.146124 8 ATTGCAC 70 0.008382051 27.14038 3 CCTTTAG 160 6.1905666E-8 26.721964 1 CTAGGAC 90 8.931199E-4 26.386478 3 GCATTCC 130 9.967858E-6 25.580002 8 TAGGCAT 150 1.0549938E-6 25.33102 5 TTAGGCA 115 1.278338E-4 24.780346 4 GCCAGAT 50 0.0016510523 23.752857 12-13 TTGCACT 105 0.002192261 22.616982 4 CCCTGGA 150 3.0222047E-5 22.164642 2 GAGTACT 1620 0.0 21.846764 12-13 AGTACTT 1615 0.0 21.767324 12-13 AGTGGAC 110 0.002872199 21.588938 3 >>END_MODULE