FastQCFastQC Report
Thu 26 May 2016
SRR937261_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937261_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264698
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16350.6176850599551187No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13330.503592773651482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.47865869783678006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8210.31016479157379356No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6920.2614300070268759No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6050.2285623616347687No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4880.18436104541779688No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4820.18209431125282396No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4150.15678244641062644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3810.14393761947577996No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3680.13902636211833863No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3570.1348706828158883No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3520.13298173767841087No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3500.13222615962341988No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3480.13147058156842892No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3270.12353701199102372No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3170.11975912171606888No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.11673680949610499No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3060.11560344241361854No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC2680.1012474593687901No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCCC200.002073022371.2437448
TTAGACT704.2945485E-640.718434
GTATAGC1456.548362E-1032.7805941
ATAGCCC1051.8928331E-631.669893
CCCGCCG300.00395256331.66088984-85
GTGTAGC1551.3369572E-930.6657161
GAATAGT650.00581692429.2503741
TAGCTTG1004.935427E-528.50295
TAGCCCT2600.027.4066334
GTAGCCC1257.3568826E-626.6027073
CTTAGAC950.001223324825.0025423
GTAGGAC1201.7047765E-423.7524173
TAGTGTT1401.7739541E-523.7479137
GTACATG10350.022.962251
AGAGTGC1050.002192889322.6170626
TACATGG10550.022.0638562
AGTACTT13300.021.96235812-13
ACAGGTC1302.9349435E-421.9211548
GAGTACT13550.021.90767112-13
GATCTAT1100.002860400821.605391
Produced by FastQC (version 0.11.5)