##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937261_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 264698 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.870675260107745 33.0 31.0 34.0 30.0 34.0 2 31.9706760156858 33.0 31.0 34.0 30.0 34.0 3 32.020835064866375 33.0 31.0 34.0 30.0 34.0 4 35.64307626049309 37.0 35.0 37.0 33.0 37.0 5 35.622241195626714 37.0 35.0 37.0 33.0 37.0 6 35.72319775744433 37.0 35.0 37.0 35.0 37.0 7 35.68018647666397 37.0 35.0 37.0 33.0 37.0 8 35.68123673016041 37.0 35.0 37.0 33.0 37.0 9 37.323776530234454 39.0 37.0 39.0 34.0 39.0 10-11 37.2543899084995 39.0 37.0 39.0 34.0 39.0 12-13 37.1728290353535 39.0 37.0 39.0 34.0 39.0 14-15 38.371298612003116 40.0 38.0 41.0 34.0 41.0 16-17 38.34072792389818 40.0 38.0 41.0 34.0 41.0 18-19 38.34564295914589 40.0 38.0 41.0 34.0 41.0 20-21 38.30197432545769 40.0 38.0 41.0 34.0 41.0 22-23 38.192730961321956 40.0 38.0 41.0 34.0 41.0 24-25 38.079290361090756 40.0 38.0 41.0 33.5 41.0 26-27 37.91811045040008 40.0 37.5 41.0 33.0 41.0 28-29 37.75904615826338 40.0 37.0 41.0 33.0 41.0 30-31 37.72938971960498 40.0 37.0 41.0 33.0 41.0 32-33 37.542344483146834 40.0 37.0 41.0 33.0 41.0 34-35 37.361921888340675 40.0 36.5 41.0 33.0 41.0 36-37 37.32450944094779 40.0 37.0 41.0 33.0 41.0 38-39 37.26313194659574 40.0 37.0 41.0 33.0 41.0 40-41 37.10376920112732 40.0 36.5 41.0 32.0 41.0 42-43 36.835778887637986 40.0 36.0 41.0 31.0 41.0 44-45 36.535735064110796 40.0 36.0 41.0 30.5 41.0 46-47 36.13273239692026 40.0 36.0 41.0 30.0 41.0 48-49 35.77322080257501 39.0 36.0 41.0 28.0 41.0 50-51 34.80272045878699 38.5 34.0 40.0 25.5 40.5 52-53 34.89177099940309 38.5 35.0 40.0 26.0 40.5 54-55 35.260717874710046 39.0 35.0 40.5 26.0 41.0 56-57 35.04949602943732 39.0 35.0 41.0 24.5 41.0 58-59 34.83650802046105 39.0 35.0 41.0 23.0 41.0 60-61 34.577669646162796 39.0 35.0 40.5 22.5 41.0 62-63 34.29807743163907 38.5 34.5 40.0 21.5 41.0 64-65 33.97527559709556 38.0 34.0 40.0 19.0 41.0 66-67 33.60765665022025 38.0 34.0 40.0 16.5 41.0 68-69 33.13773810153458 37.0 33.5 40.0 10.5 41.0 70-71 32.72921971454261 36.5 33.0 39.0 7.5 41.0 72-73 32.30510619649563 36.0 32.5 39.0 4.5 40.5 74-75 31.87897717398696 35.5 32.0 38.5 2.0 40.0 76-77 31.422734588096624 35.0 32.0 37.5 2.0 39.5 78-79 30.952842484642876 35.0 31.0 37.0 2.0 39.0 80-81 30.49220432341763 35.0 31.0 36.5 2.0 39.0 82-83 30.033060317796128 35.0 31.0 36.0 2.0 37.5 84-85 29.56661742816342 34.0 30.0 36.0 2.0 37.0 86-87 29.153686465330303 34.0 29.5 35.0 2.0 37.0 88-89 28.754886701070653 34.0 29.0 35.0 2.0 36.0 90-91 28.311062040514095 34.0 29.0 35.0 2.0 36.0 92-93 27.920688860512733 34.0 29.0 35.0 2.0 35.5 94-95 27.438871846406094 34.0 27.0 35.0 2.0 35.0 96-97 26.87972708520654 34.0 26.0 35.0 2.0 35.0 98-99 26.243877928809436 33.0 25.0 35.0 2.0 35.0 100-101 24.508944155225954 31.5 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03337596331041581 1101 2 0.08185422485520988 1101 3 0.08856956070360411 1101 4 0.12085869800216642 1101 5 0.1642678231664192 1101 6 0.14438291785760526 1101 7 -0.05220001451571932 1101 8 0.06670997910116228 1101 9 0.16720847840637276 1101 10-11 0.15290040163239382 1101 12-13 0.3244925489971351 1101 14-15 0.6186608681306041 1101 16-17 0.372383258147579 1101 18-19 0.3559405448305242 1101 20-21 0.48067989879380235 1101 22-23 0.3020117844293537 1101 24-25 0.37786247212861923 1101 26-27 0.21966080995056814 1101 28-29 0.15625127802015015 1101 30-31 0.26614098353048377 1101 32-33 0.36092523760404305 1101 34-35 0.13765295082146167 1101 36-37 0.36865227955748026 1101 38-39 0.5102118789603693 1101 40-41 0.3244094124454122 1101 42-43 0.37809390810980403 1101 44-45 0.294851561999387 1101 46-47 0.5548555016793202 1101 48-49 0.7488380452700198 1101 50-51 0.4348714306149901 1101 52-53 0.6460802142520379 1101 54-55 0.6503787627299076 1101 56-57 0.7272887490912794 1101 58-59 0.6076252624630669 1101 60-61 0.641864816137165 1101 62-63 0.75384879923174 1101 64-65 0.6229134755634718 1101 66-67 0.783627824080007 1101 68-69 0.5176090550213743 1101 70-71 0.6308390613038171 1101 72-73 0.8417893438510973 1101 74-75 0.774302132542136 1101 76-77 0.7510044386740802 1101 78-79 1.0138753204217963 1101 80-81 1.035895340335756 1101 82-83 0.727244128162333 1101 84-85 0.8061399837932974 1101 86-87 1.2241661912232225 1101 88-89 1.033210600780965 1101 90-91 0.5970006715516831 1101 92-93 0.5844641002385664 1101 94-95 0.5663682326442903 1101 96-97 0.8806634288951756 1101 98-99 0.6666899285070507 1101 100-101 1.0004452540739095 1102 1 0.2839187660983242 1102 2 0.2752162329440573 1102 3 0.4404858475367561 1102 4 0.17624163438272689 1102 5 0.3507735688170541 1102 6 0.372976356902349 1102 7 0.22752008563193726 1102 8 0.10850236531160107 1102 9 0.4235993110442351 1102 10-11 0.27796941363726546 1102 12-13 0.36261927429725205 1102 14-15 0.46080312915647426 1102 16-17 0.3855565264277345 1102 18-19 0.5317253557697228 1102 20-21 0.4865956720492406 1102 22-23 0.32527364486460897 1102 24-25 0.5905189528373498 1102 26-27 0.6139183691724952 1102 28-29 0.827144261264678 1102 30-31 0.6619264364418243 1102 32-33 0.8438198899761318 1102 34-35 1.0217288319282218 1102 36-37 0.7822781381952169 1102 38-39 1.001432909967626 1102 40-41 1.1880994682969899 1102 42-43 1.1795190862570664 1102 44-45 1.2445915632833362 1102 46-47 1.2365111478242738 1102 48-49 1.1821852879996868 1102 50-51 1.0130411680483888 1102 52-53 1.280862264711054 1102 54-55 1.5185465742468836 1102 56-57 1.9002167840145674 1102 58-59 1.8325466208761156 1102 60-61 1.722457998393061 1102 62-63 1.6448579794333185 1102 64-65 1.371209039535998 1102 66-67 1.565559518244477 1102 68-69 1.4453422514006533 1102 70-71 1.5289484533562003 1102 72-73 1.7019549726628043 1102 74-75 1.3173078460941703 1102 76-77 1.325498885615076 1102 78-79 1.2779441398350357 1102 80-81 1.2111376848180058 1102 82-83 1.4712224295040564 1102 84-85 1.6221796576714524 1102 86-87 1.565801294259753 1102 88-89 1.8403717999849185 1102 90-91 1.6433414303627494 1102 92-93 1.4889033375756604 1102 94-95 1.3355471502792646 1102 96-97 1.525290122096692 1102 98-99 1.1939416457825196 1102 100-101 1.1618819877196742 1103 1 -0.16444169811228093 1103 2 -0.15822873940581417 1103 3 0.11323294622045665 1103 4 -0.1145831962115551 1103 5 -0.09913686034409608 1103 6 -0.28880415559861916 1103 7 -0.188509200137986 1103 8 -0.23038695628665806 1103 9 -0.25749734757403786 1103 10-11 0.006343897631353457 1103 12-13 0.21780269979768008 1103 14-15 0.3413740458945398 1103 16-17 0.06381028450858395 1103 18-19 0.04294897131611464 1103 20-21 0.11572956098139997 1103 22-23 -0.010729937968946501 1103 24-25 -0.008698275918781917 1103 26-27 -0.04107423149734046 1103 28-29 -0.1669362746025982 1103 30-31 -0.22085649057741108 1103 32-33 -0.09191108595657482 1103 34-35 -0.011977064169606422 1103 36-37 0.11790786013617804 1103 38-39 0.18499471384642874 1103 40-41 0.2652939538488326 1103 42-43 -0.08791607224436859 1103 44-45 -0.29471835181909256 1103 46-47 0.028508188092210673 1103 48-49 0.384089641921328 1103 50-51 0.2130139073583024 1103 52-53 0.19514686631553246 1103 54-55 0.4058703533574075 1103 56-57 0.257845882854042 1103 58-59 -0.07521099264518938 1103 60-61 0.057819007817464296 1103 62-63 0.08400822477953085 1103 64-65 0.2166487155232204 1103 66-67 0.26903720342245663 1103 68-69 0.27538353187420483 1103 70-71 0.20213561746555087 1103 72-73 0.22204220687107323 1103 74-75 0.2760818939172047 1103 76-77 0.4910257958105788 1103 78-79 0.6428635260800704 1103 80-81 0.2996765118294533 1103 82-83 0.26570408695401326 1103 84-85 0.41280587466654595 1103 86-87 0.6809932874056379 1103 88-89 0.9901848695386235 1103 90-91 1.234668636531456 1103 92-93 0.8985029948698475 1103 94-95 0.7697709994887028 1103 96-97 0.8619245951864976 1103 98-99 0.6925786112782752 1103 100-101 1.0449803598670329 1104 1 0.3365480727212429 1104 2 0.4377237884228613 1104 3 0.18131997239643738 1104 4 0.09411573851881627 1104 5 0.3067704602772068 1104 6 0.17123212065332183 1104 7 0.22479147843048253 1104 8 0.26867851590308334 1104 9 0.3350577343237475 1104 10-11 0.2215753198629855 1104 12-13 0.3566007957548152 1104 14-15 0.3641663965772466 1104 16-17 0.337797265569435 1104 18-19 0.086245690801249 1104 20-21 0.2697793349453619 1104 22-23 0.1488165168100295 1104 24-25 0.12275710152918151 1104 26-27 0.2578135423009016 1104 28-29 0.2611741377865684 1104 30-31 0.1563612866274724 1104 32-33 0.3065476336942865 1104 34-35 0.64665327714021 1104 36-37 0.17968337090396602 1104 38-39 0.47395255421250937 1104 40-41 0.27392107264894605 1104 42-43 0.2268034313049867 1104 44-45 0.35668896419862506 1104 46-47 0.31630132138441525 1104 48-49 0.4857644262433567 1104 50-51 0.24838785658995732 1104 52-53 0.6128715903305988 1104 54-55 0.699804669402738 1104 56-57 0.4261989962587549 1104 58-59 0.34150094260999 1104 60-61 0.5089013107149682 1104 62-63 0.7602841084377232 1104 64-65 0.5785227430158386 1104 66-67 0.7654602176659466 1104 68-69 0.786901702225272 1104 70-71 1.0369313563870222 1104 72-73 1.1398359846652184 1104 74-75 0.8133617274515679 1104 76-77 0.5933773071676214 1104 78-79 0.9570996531758524 1104 80-81 0.8359757817008315 1104 82-83 1.1288210824701217 1104 84-85 1.145765703781457 1104 86-87 1.0839819349643527 1104 88-89 1.0465327532704727 1104 90-91 1.214790355326052 1104 92-93 1.2044848935725554 1104 94-95 0.737757667505754 1104 96-97 1.0249835855281724 1104 98-99 1.1609565840434648 1104 100-101 0.9704391071394127 1105 1 0.15289126282412724 1105 2 0.5121492011545605 1105 3 0.4093392824694888 1105 4 0.43461763152524924 1105 5 0.6108759619590955 1105 6 0.2526525088252001 1105 7 0.3302227883346376 1105 8 0.43544604908348106 1105 9 0.7257067047815866 1105 10-11 0.5472990957422823 1105 12-13 0.5985044987287367 1105 14-15 0.591967553492708 1105 16-17 0.4624823509306495 1105 18-19 0.5717420244207574 1105 20-21 0.5184387486059734 1105 22-23 1.1090633817380393 1105 24-25 0.8848575645768406 1105 26-27 0.8357364427526832 1105 28-29 0.8520877069129682 1105 30-31 0.9496928372038198 1105 32-33 0.7248950179675546 1105 34-35 0.7790511322020635 1105 36-37 0.9782717088583937 1105 38-39 0.8410269092532516 1105 40-41 0.5504759416866776 1105 42-43 0.6583737118077266 1105 44-45 0.7303993975493128 1105 46-47 0.7354931694935232 1105 48-49 0.6455440211822037 1105 50-51 0.6054271663815172 1105 52-53 0.4318326183245702 1105 54-55 0.6423529797713456 1105 56-57 0.3041458231858982 1105 58-59 0.5273674328775471 1105 60-61 0.6537831561499345 1105 62-63 0.6058950076509646 1105 64-65 0.5121901087108967 1105 66-67 0.5418602683337852 1105 68-69 0.7060161411494263 1105 70-71 0.33946756277399004 1105 72-73 0.5654387207280465 1105 74-75 0.1317738539522466 1105 76-77 0.2753381521944327 1105 78-79 0.20385198600719434 1105 80-81 0.05989944217007093 1105 82-83 0.3366647440653132 1105 84-85 0.26069019464014076 1105 86-87 -0.0015341028113304844 1105 88-89 0.3084572863523434 1105 90-91 0.33616795733024674 1105 92-93 0.27213348402166915 1105 94-95 -0.00133819321401063 1105 96-97 -0.3265315233372874 1105 98-99 0.10399046111767873 1105 100-101 0.7178529366421635 1106 1 -0.06472988255032064 1106 2 0.08838324611107495 1106 3 0.03771384476475248 1106 4 0.02343498157607371 1106 5 0.0763452943887799 1106 6 -0.18100839708240102 1106 7 0.18150483961668584 1106 8 0.3363609931702243 1106 9 0.001839089284018769 1106 10-11 -0.04677071110285169 1106 12-13 0.008218719896696314 1106 14-15 -0.46792174956840427 1106 16-17 -0.04354950267829594 1106 18-19 -0.07049224644786989 1106 20-21 -0.446703011249447 1106 22-23 -0.3681081985172341 1106 24-25 -0.563431151112745 1106 26-27 0.050612755866886516 1106 28-29 -0.06606433194391315 1106 30-31 -0.37809435357896604 1106 32-33 0.2943742905305342 1106 34-35 0.2990330592324497 1106 36-37 -0.23653683061975528 1106 38-39 -0.3630508045160923 1106 40-41 0.026145206342732763 1106 42-43 -0.3743270675890855 1106 44-45 -0.9746045559127836 1106 46-47 -0.7476537863406563 1106 48-49 -0.7736359178215224 1106 50-51 -0.6869934819862635 1106 52-53 -0.7599554761066898 1106 54-55 -0.6873092816089681 1106 56-57 -0.7598663760685938 1106 58-59 -0.6513064629769687 1106 60-61 -0.4974311214960636 1106 62-63 -0.9676506830753411 1106 64-65 -1.2010709327439741 1106 66-67 -1.4585915559065903 1106 68-69 -1.436986230927836 1106 70-71 -1.189381414973667 1106 72-73 -1.2897290190211876 1106 74-75 -1.213114884328565 1106 76-77 -1.4000035398873436 1106 78-79 -1.0571216952307978 1106 80-81 -1.3858477621674439 1106 82-83 -1.105388797107171 1106 84-85 -1.0699547844629933 1106 86-87 -1.1419949635365043 1106 88-89 -1.1798291702160526 1106 90-91 -1.1515650145436958 1106 92-93 -1.6611659624936372 1106 94-95 -1.7949448802127677 1106 96-97 -1.4274125806060134 1106 98-99 -1.5590729072320322 1106 100-101 -1.4339600081223232 1107 1 -0.19126049479521967 1107 2 -0.24863925519714414 1107 3 -0.03907841059893258 1107 4 0.06589965975503986 1107 5 -0.029149211105725215 1107 6 -0.05115491722892074 1107 7 0.08532482915096296 1107 8 -0.030907452669644897 1107 9 0.07172396631175104 1107 10-11 0.2506390220211614 1107 12-13 0.03803295328236089 1107 14-15 0.2624026878988843 1107 16-17 0.10574406090098165 1107 18-19 0.40786986762852706 1107 20-21 0.2632917558881829 1107 22-23 0.30126647442886423 1107 24-25 0.2706347829531879 1107 26-27 0.4820099301212011 1107 28-29 0.41263791818690976 1107 30-31 0.222337357567028 1107 32-33 0.3515694762971435 1107 34-35 0.4975286112994297 1107 36-37 0.34242706262985223 1107 38-39 -0.32895870613827327 1107 40-41 -0.3414372112396862 1107 42-43 0.21829459646114913 1107 44-45 -0.14503888348996696 1107 46-47 0.17998880915908444 1107 48-49 -0.19357312347301558 1107 50-51 -0.17641001783137256 1107 52-53 0.005834686112045517 1107 54-55 0.05843901771075366 1107 56-57 -0.28824157230093306 1107 58-59 -0.07707831017739153 1107 60-61 -0.27966555373049573 1107 62-63 -0.2995711435672348 1107 64-65 -0.050495317882649715 1107 66-67 -0.3176313256606491 1107 68-69 -0.34475703155577975 1107 70-71 -0.677973774994598 1107 72-73 -0.6430257223178906 1107 74-75 -0.4601190706184646 1107 76-77 -0.48557392188629933 1107 78-79 -0.1777784194589529 1107 80-81 -0.07140506201045582 1107 82-83 0.1538341228300304 1107 84-85 -0.2295440047664954 1107 86-87 -0.577042059297888 1107 88-89 -0.6841593638319594 1107 90-91 -0.4037628167414944 1107 92-93 0.014592802550840389 1107 94-95 -0.27180254635614887 1107 96-97 -0.4723105397896852 1107 98-99 -0.42657683187881545 1107 100-101 -0.03560835977067711 1108 1 -0.1389773741891176 1108 2 -0.4362544350483475 1108 3 -0.30453810618285004 1108 4 -0.41937438297242124 1108 5 -0.42332025745068336 1108 6 -0.3016327003154018 1108 7 -0.5092420946085028 1108 8 -0.28370589646188193 1108 9 -0.34085879254430296 1108 10-11 -0.2711854268219156 1108 12-13 -0.24685374387141934 1108 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-0.5622680738818744 2316 82-83 -0.35978799268283623 2316 84-85 -0.6827752972835057 2316 86-87 -0.9814871706757735 2316 88-89 -0.7246255503065484 2316 90-91 -0.6182819577312237 2316 92-93 -0.37094474580183245 2316 94-95 -0.48943960117807706 2316 96-97 -0.7486544106713708 2316 98-99 -1.3741558634914597 2316 100-101 -1.3766447743365013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 896.0 3 119.0 4 94.0 5 122.0 6 139.0 7 153.0 8 192.0 9 230.0 10 362.0 11 485.0 12 841.0 13 1139.0 14 1515.0 15 1993.0 16 2787.0 17 3824.0 18 2973.0 19 1905.0 20 1805.0 21 1753.0 22 1731.0 23 1888.0 24 1992.0 25 2166.0 26 2477.0 27 2819.0 28 3346.0 29 4162.0 30 4971.0 31 6381.0 32 8059.0 33 10970.0 34 14848.0 35 21621.0 36 32569.0 37 52529.0 38 58424.0 39 10416.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.503858524554765 21.968775690348195 15.297714435087 21.22965135001004 2 14.53130326099307 23.059879559141006 40.126879521266524 22.2819376585994 3 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18.996536517970362 22-23 19.470719825579412 29.65660947276382 31.21382797921185 19.658842722444916 24-25 19.724048400343662 29.732639953370953 31.590989088349176 18.95232255793621 26-27 19.613257577191394 29.562875452814545 31.98867443154504 18.835192538449018 28-29 18.770842717682196 30.83548779056783 31.360885150711066 19.03278434103891 30-31 19.66657392212866 29.975223959131835 31.289735829486325 19.06846628925318 32-33 19.242133156674395 30.11858231202385 31.34226091894194 19.29702361235982 34-35 19.862292947339732 30.32640129606638 30.870529630863334 18.94077612573055 36-37 19.597946202310048 30.013607450599654 31.257203526960353 19.13124282012994 38-39 19.28565616194418 30.428685783709824 31.27894192029582 19.006716134050176 40-41 19.770487335927207 30.482150697167427 30.738005843792106 19.009356123113257 42-43 19.27167306280325 30.658310057451686 30.279651202397982 19.79036567734708 44-45 19.753841145586897 30.318269052422792 30.244266198297364 19.68362360369295 46-47 20.021763609352675 30.581656116047352 29.77980904798403 19.61677122661595 48-49 19.9721029880463 30.21772539044461 30.230784371616963 19.57938724989212 50-51 19.491942474911323 30.74332892948672 30.162437585884472 19.602291009717483 52-53 19.888515591728577 31.187431978422374 29.108030095111907 19.81602233473714 54-55 19.78243504662106 30.31826493180037 29.574372234063095 20.324927787515474 56-57 20.11263042762473 30.57352200948427 29.118712319331486 20.195135243559513 58-59 20.50436895224616 30.358636296551385 29.095443347423473 20.041551403778982 60-61 19.85649328163761 30.060153989672212 30.06374922655101 20.019603502139166 62-63 19.71619368768438 29.72605254144598 30.231673601925596 20.326080168944046 64-65 19.4991617373797 30.05748627179388 30.042348346370872 20.401003644455546 66-67 19.65418118302055 29.56983651478823 29.858050402420773 20.91793189977045 68-69 19.557668982142893 29.293076467482727 30.170131681277944 20.979122869096432 70-71 19.707371719664227 29.292348217056613 30.0632791625351 20.93700090074406 72-73 19.2879096421569 28.781312217537113 30.765081720251025 21.16569642005496 74-75 19.128152433289618 29.936359972068782 29.936549207953206 20.99893838668839 76-77 19.286976862138008 29.324675717924748 29.91362571395047 21.474721705986774 78-79 19.02615343855267 29.32250861057492 29.917489875477838 21.733848075394572 80-81 19.363561361812927 29.172217260327887 29.77716889447312 21.68705248338606 82-83 19.266799900074943 28.857523524023648 30.218056154853556 21.657620421047852 84-85 19.253300938908506 28.462437983219992 30.22180341579531 22.062457662076195 86-87 19.39775155027978 28.39041504164041 29.79394572459613 22.417887683483677 88-89 19.54529369130006 27.718009420376248 29.602841630569106 23.13385525775459 90-91 19.31440358031583 27.175770420762802 30.4315491678415 23.078276831079865 92-93 19.451898729386116 27.748927907707333 30.47164490940678 22.32752845349977 94-95 19.134445066840264 28.45215205970963 29.90079935809123 22.51260351535888 96-97 18.94779469090854 29.34344084230924 29.22857705146352 22.480187415318703 98-99 19.90814072976456 29.22975604494178 28.226760304835008 22.635342920458655 100-101 20.185282948178678 28.420648301099927 28.65414417329405 22.739924577427345 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 406.0 1 247.0 2 95.5 3 108.0 4 85.0 5 68.0 6 112.5 7 179.5 8 265.0 9 298.5 10 260.0 11 227.5 12 222.0 13 243.0 14 289.0 15 321.5 16 357.0 17 413.5 18 495.5 19 594.0 20 702.0 21 845.0 22 990.5 23 1217.5 24 1471.0 25 1825.0 26 2356.0 27 3017.0 28 3721.5 29 4545.5 30 5371.0 31 6244.5 32 7106.0 33 7840.5 34 8652.5 35 9416.0 36 10343.5 37 10985.5 38 10849.0 39 10461.5 40 10385.0 41 10427.5 42 10206.5 43 9990.0 44 9848.5 45 9784.0 46 9935.5 47 10728.5 48 12740.5 49 12085.5 50 8872.0 51 6905.5 52 5737.5 53 4897.0 54 4169.0 55 3484.5 56 2815.0 57 2246.5 58 1785.5 59 1374.0 60 1000.5 61 711.0 62 493.0 63 331.5 64 212.0 65 141.0 66 98.5 67 69.0 68 44.5 69 36.0 70 30.0 71 16.0 72 12.0 73 11.0 74 8.0 75 5.0 76 1.5 77 1.5 78 1.0 79 1.5 80 2.5 81 2.0 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27843051326417273 2 0.25236307036698424 3 0.2561409606419391 4 0.2550075935594526 5 0.2485851800920294 6 0.19267240402269759 7 0.2024949187375802 8 0.20098376262759823 9 0.19833923943512988 10-11 0.2055172309575441 12-13 0.20438386387505758 14-15 0.18946119728898594 16-17 0.20117265714134597 18-19 0.18889451374774271 20-21 0.19380577110518402 22-23 0.1922946149952021 24-25 0.18322767833531042 26-27 0.19531692721516594 28-29 0.19531692721516594 30-31 0.20079486811385047 32-33 0.20230602422383245 34-35 0.1941835601326795 36-37 0.1905945643714724 38-39 0.1826609947940672 40-41 0.18360546736280592 42-43 0.17982757708785105 44-45 0.19607250527015693 46-47 0.18719446312401303 48-49 0.19342798207768855 50-51 0.2021171297100847 52-53 0.20230602422383245 54-55 0.20665059804003053 56-57 0.17793863195037363 58-59 0.168871695290482 60-61 0.17359405813417556 62-63 0.1769941593816349 64-65 0.17416074167541878 66-67 0.1830387838215627 68-69 0.17434963618916652 70-71 0.17831642097786912 72-73 0.19040566985772464 74-75 0.18039426062909428 76-77 0.18851672472024725 78-79 0.18436104541779688 80-81 0.17453853070291428 82-83 0.18889451374774271 84-85 0.17189400751044587 86-87 0.1781275264641214 88-89 0.1826609947940672 90-91 0.17963868257410331 92-93 0.1870055686102653 94-95 0.18360546736280592 96-97 0.17831642097786912 98-99 0.1849277289590401 100-101 0.17132732396920264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 264698.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.75605350668309 #Duplication Level Percentage of deduplicated Percentage of total 1 89.34218659036655 70.36238027514972 2 8.065328794222793 12.703869321336041 3 1.5107138616655775 3.5693358516786615 4 0.4762928172002757 1.5004377038509498 5 0.1521643738905463 0.5991932785967398 6 0.09922102767761155 0.4688553938859636 7 0.05945253949512794 0.327757316711054 8 0.03435685406948483 0.21646481899341197 9 0.029886229477880444 0.21183493390856653 >10 0.18721802888079123 2.982495843054905 >50 0.02015014542489929 1.1433567590392764 >100 0.020150145424899256 3.513573860643165 >500 0.0014392961017785182 0.8002741624736234 >1k 0.0014392961017785182 1.6001704806779014 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1635 0.6176850599551187 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1333 0.503592773651482 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1267 0.47865869783678006 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 821 0.31016479157379356 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 692 0.2614300070268759 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 605 0.2285623616347687 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 488 0.18436104541779688 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 482 0.18209431125282396 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 415 0.15678244641062644 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 381 0.14393761947577996 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 368 0.13902636211833863 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 357 0.1348706828158883 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 352 0.13298173767841087 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 350 0.13222615962341988 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 348 0.13147058156842892 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 327 0.12353701199102372 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 317 0.11975912171606888 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 309 0.11673680949610499 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 306 0.11560344241361854 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 268 0.1012474593687901 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.8889451374774272E-4 0.0 20-21 0.0 0.0 0.0 5.666835412432282E-4 0.0 22-23 0.0 0.0 0.0 0.001322261596234199 0.0 24-25 0.0 0.0 0.0 0.00207783965122517 0.0 26-27 0.0 0.0 0.0 0.004533468329945825 0.0 28-29 0.0 0.0 0.0 0.008500253118648422 0.0 30-31 0.0 0.0 0.0 0.017000506237296844 0.0 32-33 0.0 0.0 0.0 0.02776749352091818 0.0 34-35 0.0 0.0 0.0 0.037023324694557574 0.0 36-37 0.0 0.0 0.0 0.04609026135444922 0.0 38-39 0.0 0.0 0.0 0.06384634564673704 0.0 40-41 0.0 0.0 0.0 0.1105032905424295 0.0 42-43 0.0 0.0 0.0 0.18587220152777884 0.0 44-45 0.0 0.0 0.0 0.259918850916894 0.0 46-47 0.0 0.0 0.0 0.440502006059736 0.0 48-49 0.0 0.0 0.0 0.6040846549652812 0.0 50-51 0.0 0.0 0.0 0.708543321067783 0.0 52-53 0.0 0.0 0.0 0.8409583752049505 0.0 54-55 0.0 0.0 0.0 0.9612841804622627 0.0 56-57 0.0 0.0 0.0 1.3131946595743074 0.0 58-59 0.0 0.0 0.0 1.7308404294705664 0.0 60-61 0.0 0.0 0.0 2.1469750432568437 0.0 62-63 0.0 0.0 0.0 2.627333791717353 0.0 64-65 0.0 0.0 0.0 3.271464083597156 0.0 66-67 0.0 0.0 0.0 4.15567930245034 0.0 68-69 0.0 0.0 0.0 5.179298672449358 0.0 70-71 0.0 0.0 0.0 5.872352643389825 0.0 72-73 0.0 0.0 0.0 6.444514125531738 0.0 74-75 0.0 0.0 0.0 7.264882998738185 0.0 76-77 0.0 0.0 0.0 8.334025946550408 0.0 78-79 0.0 0.0 0.0 9.320621991854868 0.0 80-81 0.0 0.0 0.0 10.289650847380788 0.0 82-83 0.0 0.0 0.0 11.465896984487983 0.0 84-85 0.0 0.0 0.0 13.295907033676114 0.0 86-87 0.0 0.0 0.0 15.459127005115263 0.0 88-89 0.0 0.0 0.0 17.698471465594753 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGCCC 20 0.0020730223 71.243744 8 TTAGACT 70 4.2945485E-6 40.71843 4 GTATAGC 145 6.548362E-10 32.780594 1 ATAGCCC 105 1.8928331E-6 31.66989 3 CCCGCCG 30 0.003952563 31.660889 84-85 GTGTAGC 155 1.3369572E-9 30.665716 1 GAATAGT 65 0.005816924 29.250374 1 TAGCTTG 100 4.935427E-5 28.5029 5 TAGCCCT 260 0.0 27.406633 4 GTAGCCC 125 7.3568826E-6 26.602707 3 CTTAGAC 95 0.0012233248 25.002542 3 GTAGGAC 120 1.7047765E-4 23.752417 3 TAGTGTT 140 1.7739541E-5 23.747913 7 GTACATG 1035 0.0 22.96225 1 AGAGTGC 105 0.0021928893 22.617062 6 TACATGG 1055 0.0 22.063856 2 AGTACTT 1330 0.0 21.962358 12-13 ACAGGTC 130 2.9349435E-4 21.921154 8 GAGTACT 1355 0.0 21.907671 12-13 GATCTAT 110 0.0028604008 21.60539 1 >>END_MODULE