FastQCFastQC Report
Thu 26 May 2016
SRR937261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264698
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20340.7684228819258174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.6599974310346131No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11620.438990849949754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.4065009935851423No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8260.31205373671127096No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6940.2621855850818669No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5460.20627280901253506No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5090.1922946149952021No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.16282707085055423No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4100.15489350127314902No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3960.14960445488821222No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.1375152060083567No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3510.1326039486509154No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3160.1193813326885734No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3110.11749238755109598No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3100.11711459852360047No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3020.1140922863036366No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG2990.11295891922115013No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.11106997408367272No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA2760.10426977158875397No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2750.10389198256125849No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT2680.1012474593687901No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC608.265504E-539.5897873
CATTCCG756.9033467E-637.999029
CTAGACT1201.4962461E-731.6718314
GAATAGC600.003946006331.665856
TTTAGGC1354.1984276E-728.1527393
CATATAG700.0083734427.1472853
GGCATTC1457.850904E-726.2062197
TTAGGCA1109.439154E-525.9133174
TGTAGGT950.001222897225.0040782
GTACATG9000.024.8142911
TACATGG8750.024.4325562
TCTTAGG1401.7705373E-523.7538762
GACCATC1000.00165054423.7493867
GAGTACT17100.023.47161512-13
CCTTTAG1852.5177542E-723.1162431
TAGGCAT1652.4147284E-623.0340585
GCATTCC1652.4186993E-623.0297078
CATGGGG4400.022.6741524
AGTACAT6600.022.3142432
ACATGGG9150.021.8068353
Produced by FastQC (version 0.11.5)