##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937261_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 264698 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.086026339450996 31.0 31.0 34.0 31.0 34.0 2 32.31517805196866 34.0 31.0 34.0 31.0 34.0 3 32.387426425586895 34.0 31.0 34.0 30.0 34.0 4 36.017053396701144 37.0 35.0 37.0 35.0 37.0 5 35.909368412303834 37.0 35.0 37.0 35.0 37.0 6 35.99513407732586 37.0 35.0 37.0 35.0 37.0 7 35.95217946489962 37.0 35.0 37.0 35.0 37.0 8 35.932753553105805 37.0 35.0 37.0 35.0 37.0 9 37.62011046551164 39.0 37.0 39.0 35.0 39.0 10-11 37.56665331812103 39.0 37.0 39.0 35.0 39.0 12-13 37.503407657028006 39.0 37.0 39.0 35.0 39.0 14-15 38.68292922500359 40.0 38.0 41.0 34.0 41.0 16-17 38.669606494948965 40.0 38.0 41.0 34.5 41.0 18-19 38.73288993494473 40.0 38.0 41.0 35.0 41.0 20-21 38.65863927948077 40.0 38.0 41.0 34.0 41.0 22-23 38.55022894015066 40.0 38.0 41.0 34.0 41.0 24-25 38.41930237478182 40.0 38.0 41.0 34.0 41.0 26-27 38.32942636514065 40.0 38.0 41.0 34.0 41.0 28-29 38.251074809783226 40.0 38.0 41.0 34.0 41.0 30-31 38.254193458205194 40.0 38.0 41.0 34.0 41.0 32-33 38.10287950796757 40.0 37.0 41.0 34.0 41.0 34-35 37.95483721070805 40.0 37.0 41.0 33.0 41.0 36-37 37.77933342903989 40.0 37.0 41.0 33.0 41.0 38-39 37.60447755555388 40.0 37.0 41.0 33.0 41.0 40-41 37.40059804003052 40.0 37.0 41.0 33.0 41.0 42-43 37.12061481386335 40.0 36.5 41.0 32.5 41.0 44-45 36.71598954279972 40.0 36.0 41.0 31.0 41.0 46-47 36.22738177092384 40.0 36.0 41.0 30.0 41.0 48-49 35.810060899591235 40.0 36.0 41.0 28.5 41.0 50-51 35.54044231539339 39.0 36.0 41.0 27.0 41.0 52-53 35.30322102924843 39.0 35.0 41.0 26.0 41.0 54-55 35.078502670968426 39.0 35.0 41.0 25.5 41.0 56-57 34.825941261362004 39.0 35.0 40.0 23.5 41.0 58-59 34.61594911937378 39.0 35.0 40.0 23.0 41.0 60-61 34.29596559097538 38.0 34.0 40.0 22.0 41.0 62-63 33.961391850335104 38.0 34.0 40.0 19.5 41.0 64-65 33.65394713975927 38.0 34.0 40.0 18.0 41.0 66-67 33.24173208713326 37.5 33.5 40.0 13.5 41.0 68-69 32.86240545829587 37.0 33.0 39.0 9.5 41.0 70-71 32.45276881578252 36.0 32.5 39.0 7.5 40.0 72-73 32.026392341460834 36.0 32.0 39.0 4.5 40.0 74-75 31.606343833349705 35.0 31.5 38.0 2.0 40.0 76-77 30.190418892473687 34.0 30.0 36.0 2.0 39.0 78-79 30.80911076018708 35.0 31.0 37.0 2.0 39.0 80-81 30.641536770206045 35.0 31.0 36.5 2.0 39.0 82-83 30.288028243507696 35.0 31.0 36.0 2.0 37.5 84-85 29.913484423758398 34.0 31.0 35.5 2.0 37.0 86-87 29.52271267633303 34.0 30.0 35.0 2.0 37.0 88-89 29.09387868438749 34.0 29.5 35.0 2.0 36.0 90-91 28.67096842439308 34.0 29.0 35.0 2.0 36.0 92-93 28.36459852360048 34.0 29.0 35.0 2.0 35.5 94-95 27.998611625323953 34.0 29.0 35.0 2.0 35.0 96-97 27.550865514661993 34.0 28.5 35.0 2.0 35.0 98-99 27.104987570740995 34.0 27.0 35.0 2.0 35.0 100-101 25.38281928839659 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14770206008146403 1101 2 0.15636454488468843 1101 3 0.08723621390803515 1101 4 0.15974533771339594 1101 5 0.18868659858573977 1101 6 0.19111877858154713 1101 7 0.18048339762798093 1101 8 0.06044725850637178 1101 9 0.029406669803613283 1101 10-11 0.11155509862611979 1101 12-13 0.10618119993990405 1101 14-15 0.280893977701524 1101 16-17 0.11892553849153131 1101 18-19 -0.03999084527932695 1101 20-21 0.17920121380856813 1101 22-23 0.19973333724834674 1101 24-25 0.21567338466187636 1101 26-27 0.1040830935998045 1101 28-29 0.11459460649299302 1101 30-31 0.3342586201597939 1101 32-33 0.34015613788319143 1101 34-35 0.4759577312084602 1101 36-37 0.3141879313762601 1101 38-39 0.37004176215595663 1101 40-41 0.23713732848067082 1101 42-43 0.6380396501211365 1101 44-45 0.7590008947911997 1101 46-47 0.7732149058624387 1101 48-49 0.992883844704842 1101 50-51 1.0085747369174172 1101 52-53 1.044280559406367 1101 54-55 1.1573241959025111 1101 56-57 1.0197224584687703 1101 58-59 0.8953820242280415 1101 60-61 0.9912288603396107 1101 62-63 1.127725847782024 1101 64-65 1.16918431525972 1101 66-67 1.2564575931803361 1101 68-69 1.2460312445625306 1101 70-71 1.1114363340107687 1101 72-73 1.2337490862991451 1101 74-75 1.349015106516834 1101 76-77 1.0939167960912677 1101 78-79 1.3494524023535739 1101 80-81 1.5433773637573758 1101 82-83 1.611471747450203 1101 84-85 1.447535463525508 1101 86-87 1.6064442227769398 1101 88-89 1.5777618021376867 1101 90-91 1.8707189096094972 1101 92-93 1.3602528646695298 1101 94-95 1.6180694187932687 1101 96-97 1.6619555301786484 1101 98-99 1.706399651627958 1101 100-101 1.4633598041920486 1102 1 0.2191022013153372 1102 2 0.198587052427861 1102 3 0.25951585697152524 1102 4 0.14621898475711248 1102 5 -0.023196305696217223 1102 6 0.13221783488250338 1102 7 0.2555748786577041 1102 8 0.06888277240506824 1102 9 0.2873382355736567 1102 10-11 0.25351439759253935 1102 12-13 0.12746578618886417 1102 14-15 0.0726671047478149 1102 16-17 0.13405361193314036 1102 18-19 0.343163805808814 1102 20-21 0.2320387306603422 1102 22-23 0.16670708058788364 1102 24-25 0.38982437650882673 1102 26-27 0.2591257847470061 1102 28-29 0.21133017816918453 1102 30-31 0.32782605933292785 1102 32-33 0.23545690183003387 1102 34-35 0.20356895288144727 1102 36-37 0.2665600184276826 1102 38-39 0.25829688188375854 1102 40-41 0.17668923192806574 1102 42-43 0.47852241316922317 1102 44-45 0.4981162571844706 1102 46-47 0.8074159715361944 1102 48-49 0.1051968703195385 1102 50-51 0.02426453598014433 1102 52-53 0.15847135352590414 1102 54-55 0.08786281052663014 1102 56-57 0.2172091375468952 1102 58-59 0.17241868083713996 1102 60-61 0.3627188776728616 1102 62-63 0.10746841695452503 1102 64-65 0.1099591756307845 1102 66-67 0.34492456371381763 1102 68-69 0.15328552972655274 1102 70-71 0.1070645037482052 1102 72-73 0.05880204900056185 1102 74-75 0.22470683149596837 1102 76-77 0.4986994885224121 1102 78-79 0.5603081512140697 1102 80-81 0.4558669317101298 1102 82-83 0.5422478917655376 1102 84-85 0.432696277546178 1102 86-87 0.4292984309043071 1102 88-89 0.3442251776271874 1102 90-91 0.7081972430498276 1102 92-93 0.744932199586394 1102 94-95 0.41311660374544346 1102 96-97 0.44172056837598817 1102 98-99 0.5541334899793746 1102 100-101 0.9096171066251237 1103 1 0.3468847388961436 1103 2 0.19174072483225757 1103 3 0.1807558289319573 1103 4 0.2652235801305878 1103 5 0.23387200180436452 1103 6 -0.02686776368436483 1103 7 0.13104025758129723 1103 8 0.03501720655103213 1103 9 0.12310426096504301 1103 10-11 0.1219153633998431 1103 12-13 0.10769399710871141 1103 14-15 0.15065993908070396 1103 16-17 -0.023205520398036583 1103 18-19 0.23363035304769397 1103 20-21 0.17254422174219997 1103 22-23 0.15663230840936393 1103 24-25 0.07767000311525862 1103 26-27 0.1955149116260415 1103 28-29 0.03690459985311634 1103 30-31 0.09096181756954991 1103 32-33 0.23914098937579098 1103 34-35 0.022519026874746828 1103 36-37 -0.09006432926295105 1103 38-39 0.03565882596040382 1103 40-41 -0.09348757311905587 1103 42-43 0.11845542975930101 1103 44-45 0.06506851203297259 1103 46-47 0.2154890301856156 1103 48-49 0.5453012398187198 1103 50-51 0.43116538495592494 1103 52-53 0.7994675370292939 1103 54-55 0.6620508314005349 1103 56-57 0.419970256014075 1103 58-59 0.459645100340218 1103 60-61 0.23880735808410236 1103 62-63 0.40985567452909777 1103 64-65 0.4019873709730959 1103 66-67 0.8156894207899157 1103 68-69 0.9476542500318743 1103 70-71 0.9777865225970217 1103 72-73 0.8811441317172815 1103 74-75 1.083882000901685 1103 76-77 0.9704184697514826 1103 78-79 0.8088088132594002 1103 80-81 0.3162182051737936 1103 82-83 0.2455425408815124 1103 84-85 0.46280376255279876 1103 86-87 0.7067577345883969 1103 88-89 0.7353459814281074 1103 90-91 0.5647437030545 1103 92-93 0.6133760036209779 1103 94-95 0.6384339960657144 1103 96-97 0.8308240811106238 1103 98-99 0.7008310520948022 1103 100-101 0.8918236647682818 1104 1 0.03288339464924661 1104 2 0.09893244574450222 1104 3 0.2031476676959869 1104 4 0.1467457095649891 1104 5 0.17829752039824598 1104 6 -0.09802998482218328 1104 7 0.05066165872850803 1104 8 -0.00902069016292728 1104 9 -0.07345962298778375 1104 10-11 0.030442124041037744 1104 12-13 0.14593137637969278 1104 14-15 0.36911387100288806 1104 16-17 0.27679171745079856 1104 18-19 -0.08911319963113584 1104 20-21 -0.2002771315277272 1104 22-23 -0.034886046085119915 1104 24-25 0.1408173822941663 1104 26-27 0.12422379010059359 1104 28-29 0.3038480954474778 1104 30-31 0.0562691989581765 1104 32-33 -0.15412948967328788 1104 34-35 -0.1753706896364733 1104 36-37 -0.251383164371731 1104 38-39 -0.29373230385149185 1104 40-41 -0.2893931443779536 1104 42-43 -0.4124714560994036 1104 44-45 -0.23827092872348743 1104 46-47 -0.5247444636593883 1104 48-49 -0.7385558280657492 1104 50-51 -0.9077965986368994 1104 52-53 -0.9369219631134271 1104 54-55 -1.2569260678085712 1104 56-57 -1.022558585447662 1104 58-59 -1.0445487658427624 1104 60-61 -0.7785987978301137 1104 62-63 -0.7250892205551906 1104 64-65 -0.8499622473896906 1104 66-67 -0.836592576429517 1104 68-69 -0.9682842828975744 1104 70-71 -1.084905101639933 1104 72-73 -0.6155827191541725 1104 74-75 -0.6754701825788096 1104 76-77 -0.3145204406719593 1104 78-79 -0.46127893107588847 1104 80-81 -0.671457997052503 1104 82-83 -0.3977486543012958 1104 84-85 -0.02007550381985368 1104 86-87 -0.1823326890335224 1104 88-89 -0.12327331127705321 1104 90-91 -0.26962850602192745 1104 92-93 -0.010179621499865732 1104 94-95 -0.0522879381766721 1104 96-97 0.049940929312004556 1104 98-99 0.16116656138944307 1104 100-101 0.4583132468548108 1105 1 0.12844855207137584 1105 2 0.06763098622950281 1105 3 0.20758110283413345 1105 4 -0.10715641707643897 1105 5 -0.17910772372217565 1105 6 -0.015835504800790545 1105 7 0.04746677054959747 1105 8 -0.11373420580662241 1105 9 -0.4015056744156311 1105 10-11 0.0821725711846355 1105 12-13 0.14296167855700048 1105 14-15 -0.09874020564628694 1105 16-17 -0.15408188038737336 1105 18-19 -0.31641471544372024 1105 20-21 -0.042559435788042776 1105 22-23 -0.08169749138497195 1105 24-25 -0.13199369707135844 1105 26-27 -0.17708090652576658 1105 28-29 -0.17579328891023493 1105 30-31 -0.16105356919427294 1105 32-33 -0.38773633546011865 1105 34-35 -0.31393313069992956 1105 36-37 -0.4539067038011524 1105 38-39 -0.9358274186282003 1105 40-41 -0.8825797286624777 1105 42-43 -0.8868067926886098 1105 44-45 -0.7701775873208234 1105 46-47 -1.343226962146396 1105 48-49 -1.0307121664705576 1105 50-51 -1.2339566808313833 1105 52-53 -1.7169602454739774 1105 54-55 -1.6842529175362273 1105 56-57 -1.4807013279847112 1105 58-59 -1.5861597213535958 1105 60-61 -1.3777740381504486 1105 62-63 -1.4820243798069939 1105 64-65 -1.5997265012355157 1105 66-67 -1.5530216203557146 1105 68-69 -1.355358356450374 1105 70-71 -0.9338094098129375 1105 72-73 -0.8894001179240725 1105 74-75 -1.2479744400839827 1105 76-77 -1.106324919145166 1105 78-79 -1.1084619404635099 1105 80-81 -0.7299230432868953 1105 82-83 -0.7858149728612034 1105 84-85 -0.8820971502428456 1105 86-87 -0.3987406213638174 1105 88-89 -0.270062237541282 1105 90-91 -0.17962273551905028 1105 92-93 0.11211281632648351 1105 94-95 -0.2698992314730333 1105 96-97 0.1217062809608187 1105 98-99 0.6070505039096012 1105 100-101 0.7606172256869179 1106 1 -0.4463170641039298 1106 2 -0.3632286093878001 1106 3 -0.23473223727656745 1106 4 0.05425779279592291 1106 5 -0.32721570971562386 1106 6 -0.4099166072519367 1106 7 -0.3166357935529689 1106 8 -0.31628701276471816 1106 9 -0.477457329221906 1106 10-11 -0.30386605978791437 1106 12-13 -0.36030275157968106 1106 14-15 -0.6461478640671601 1106 16-17 -0.5442208163412872 1106 18-19 -0.5063164436714374 1106 20-21 -0.16702571840410485 1106 22-23 0.005549769430572837 1106 24-25 -0.36994693326248296 1106 26-27 -0.19132466570344775 1106 28-29 -0.038947022108018814 1106 30-31 0.03884477035163769 1106 32-33 -0.4785417121685853 1106 34-35 -0.7910256417131478 1106 36-37 -0.5790394271717645 1106 38-39 -0.5400745664876823 1106 40-41 -0.9289933737545368 1106 42-43 -0.5366543020074914 1106 44-45 -1.275757616689404 1106 46-47 -1.4471578330376147 1106 48-49 -1.629175964053296 1106 50-51 -1.3779530662374597 1106 52-53 -1.3297129846584355 1106 54-55 -1.1995245768607532 1106 56-57 -1.1089516386138811 1106 58-59 -1.3306305222120614 1106 60-61 -1.6105312955773172 1106 62-63 -1.543709684223579 1106 64-65 -1.500539784868181 1106 66-67 -1.6361911674019147 1106 68-69 -1.625300748859729 1106 70-71 -1.8891239924402967 1106 72-73 -2.0567558165573026 1106 74-75 -1.6422023854132455 1106 76-77 -1.5735111836882574 1106 78-79 -1.2326232417173237 1106 80-81 -1.0872030613598689 1106 82-83 -0.7784728289551843 1106 84-85 -1.1312330363831329 1106 86-87 -1.1872642856584115 1106 88-89 -1.1762183428163304 1106 90-91 -1.3880605532079677 1106 92-93 -0.9486575440033498 1106 94-95 -1.7583664177375802 1106 96-97 -1.3479537212982997 1106 98-99 -1.0813827955369106 1106 100-101 -0.9965159495079341 1107 1 -0.0718795968093211 1107 2 -0.016002026446933826 1107 3 -0.17934238902957844 1107 4 -0.09906507481265692 1107 5 -0.18728896978888088 1107 6 -0.04042419490238558 1107 7 -0.20253322945040253 1107 8 -0.10009130371186359 1107 9 -0.019032420797003624 1107 10-11 -0.20060860295902927 1107 12-13 -0.4324900880527309 1107 14-15 -0.4013676442869425 1107 16-17 -0.28719569860188443 1107 18-19 -0.29823167130094674 1107 20-21 -0.4374731281372277 1107 22-23 -0.4794319155511175 1107 24-25 -0.530818204848039 1107 26-27 -0.37057636638371605 1107 28-29 -0.39713645172601986 1107 30-31 -0.4369009125369061 1107 32-33 -0.5732303568572306 1107 34-35 -0.6996991111009052 1107 36-37 -0.6231393748431415 1107 38-39 -0.3436198307472367 1107 40-41 -0.1708562727602967 1107 42-43 -0.5980357776746814 1107 44-45 -0.39497530376423384 1107 46-47 -0.5322834217346255 1107 48-49 -0.7798693183180845 1107 50-51 -1.2097950337937178 1107 52-53 -0.8800007920890991 1107 54-55 -0.7519055292417107 1107 56-57 -0.7419055894249666 1107 58-59 -0.9385713908672102 1107 60-61 -1.0748431741749016 1107 62-63 -1.0956065968347772 1107 64-65 -1.4187501459356824 1107 66-67 -1.4419342338592642 1107 68-69 -1.7480244537262877 1107 70-71 -1.9140193351927834 1107 72-73 -1.6859028599900618 1107 74-75 -1.6999208574174354 1107 76-77 -1.9252249461163053 1107 78-79 -1.4717519701317663 1107 80-81 -1.6171611984608596 1107 82-83 -1.6959898357579064 1107 84-85 -1.1802518746876878 1107 86-87 -1.7798990318645842 1107 88-89 -1.5498316342867682 1107 90-91 -1.2637277431608744 1107 92-93 -0.9805780044952392 1107 94-95 -1.1848839478265383 1107 96-97 -1.093152570068579 1107 98-99 -1.1190463224861524 1107 100-101 -0.7349774879694699 1108 1 -0.24720892586535825 1108 2 -0.259995610502628 1108 3 -0.2846764959164787 1108 4 -0.04719148256639727 1108 5 -0.23936732621506707 1108 6 -0.16331683021737575 1108 7 -0.2635700187480623 1108 8 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50-51 -0.4483904719689846 2316 52-53 -0.534162456754963 2316 54-55 -0.30932849842938026 2316 56-57 -0.7414554093529446 2316 58-59 -0.764818556400094 2316 60-61 -0.5078163634506154 2316 62-63 -0.3119097847766241 2316 64-65 -0.5596398351447007 2316 66-67 -1.0373223891213712 2316 68-69 -0.9879704321176455 2316 70-71 -1.0659967928425047 2316 72-73 -1.0917985516000392 2316 74-75 -1.0318202838546746 2316 76-77 -0.9113694038994211 2316 78-79 -0.9433572789219475 2316 80-81 -0.931220155376959 2316 82-83 -1.0774924367983196 2316 84-85 -1.245461291787862 2316 86-87 -1.3469425159248765 2316 88-89 -0.7132470004332241 2316 90-91 -0.5923591957419063 2316 92-93 -0.7328455781913839 2316 94-95 -0.49959316484667937 2316 96-97 -0.8481045508608958 2316 98-99 -1.3555909403332898 2316 100-101 -1.3624284683616246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 6.0 6 4.0 7 26.0 8 75.0 9 154.0 10 284.0 11 543.0 12 755.0 13 1116.0 14 1490.0 15 2281.0 16 3165.0 17 4317.0 18 3653.0 19 2210.0 20 1610.0 21 1470.0 22 1470.0 23 1543.0 24 1636.0 25 1845.0 26 2205.0 27 2597.0 28 3170.0 29 4076.0 30 5018.0 31 6277.0 32 8142.0 33 10637.0 34 14348.0 35 20865.0 36 31990.0 37 55393.0 38 62165.0 39 8156.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.18385290860087 21.560200043093182 16.223827506303323 21.032119542002622 2 14.96309001201369 22.62502927864963 40.23037574896674 22.18150496036993 3 18.66013343508451 25.450891204315862 33.04520623503011 22.843769125569516 4 11.967223023974492 19.164481786790986 39.680692713960816 29.18760247527371 5 11.697203507989707 38.80553026037868 36.440168824838565 13.057097406793048 6 28.10939259080159 39.1971227587666 18.38548081209529 14.30800383833652 7 24.71042471042471 35.28209506683088 22.34659876538546 17.66088145735895 8 23.923490166151616 35.17518077204966 21.716446667522987 19.18488239427574 9 24.138829911824043 17.351850032867645 23.21173563835012 35.297584416958195 10-11 22.81354600336988 27.72480336081119 29.535546169597048 19.926104466221883 12-13 24.570918006898555 26.063779548382467 29.454877915169646 19.910424529549328 14-15 21.028115059426213 28.032134734678767 29.525345865854675 21.414404340040345 16-17 18.820315982742596 30.708769994484282 30.659846315423618 19.81106770734951 18-19 18.41494835623994 29.378385934158928 33.56882182713885 18.637843882462278 20-21 19.744387943996557 27.669835057310593 33.456996275000186 19.128780723692664 22-23 19.11999440858509 27.988620715058854 33.35203434942187 19.539350526934186 24-25 19.513669428949758 27.7767178636665 33.72843993025975 18.981172777123987 26-27 19.187089851940563 27.796940583548363 34.30301748076058 18.712952083750487 28-29 18.589182929801066 28.95514831865228 33.73104103580314 18.72462771574352 30-31 19.39908877286568 28.1216707341952 33.6651580291502 18.81408246378892 32-33 18.902673990736613 28.23916312174629 33.64513521069294 19.213027676824154 34-35 19.704908990623277 28.27713091900959 33.14815374502263 18.869806345344507 36-37 19.48446909307966 27.907464355605256 33.51498689072074 19.093079660594338 38-39 19.10535780398794 28.650575372688873 33.39409440192219 18.84997242140099 40-41 19.4644462746224 28.718955186665557 33.0419950282964 18.774603510415645 42-43 19.167168876705702 28.605757877455712 32.636725502383214 19.590347743455368 44-45 19.62160651006052 28.637352756726536 32.11395628225374 19.627084450959206 46-47 20.086664802907464 28.613552047994318 31.784335355763925 19.51544779333429 48-49 19.778577850984895 28.507015542240595 31.85007064654814 19.86433596022637 50-51 19.42175611451541 29.051787319889083 31.8032248071387 19.72323175845681 52-53 19.720965024291832 29.593725679831355 30.65021269522248 20.03509660065433 54-55 19.78745589313104 28.637352756726536 31.017234735434347 20.557956614708083 56-57 20.207935080733517 29.095799741592305 30.37669343931575 20.31957173835843 58-59 20.43517518077205 28.8955715570197 30.486818940830684 20.18243432137757 60-61 19.9079705929021 28.51778252952421 31.38916803300365 20.18507884457004 62-63 19.840346356980408 28.390467627258232 31.22841880180432 20.540767213957036 64-65 19.503358544454436 28.684954174190963 31.05841373943135 20.75327354192325 66-67 19.572682830999856 28.09125871748181 30.73710417154644 21.598954279971892 68-69 19.48692472175838 27.922764811218826 31.34798902900664 21.242321438016155 70-71 19.723987336511797 27.803950162071494 31.18969542648603 21.282367074930676 72-73 19.226700718179654 27.471788497791817 31.743654064836395 21.557856719192134 74-75 18.865083982500813 28.45374728936373 30.916742854120542 21.764425874014915 76-77 18.927419172037567 27.68230209521795 31.15550551949769 22.234773213246793 78-79 18.827305079751262 27.85797399300335 31.08126997559483 22.23345095165056 80-81 18.870373028885748 27.63432288872602 30.660979682506103 22.83432439988213 82-83 18.421937453248606 27.489818585708996 31.267708860663852 22.820535100378546 84-85 18.31341095755854 27.10280550371372 31.084950924463723 23.498832614264018 86-87 18.31124526819243 26.9541137447204 30.78753900671709 23.947101980370082 88-89 18.5817422118792 26.487544295763477 30.346470317116108 24.58424317524122 90-91 18.134623994740632 25.914297211060543 31.037648699042776 24.913430095156052 92-93 17.462329355806954 25.9246946019105 31.696232085547354 24.91674395673519 94-95 16.99823950313187 26.6399821683579 31.250141670885313 25.111636657624913 96-97 16.899258777928054 27.469417978224243 30.666646517918533 24.964676725929174 98-99 17.229446387959108 27.41747198694361 29.60770387384869 25.745377751248594 100-101 16.208383790104868 26.27165233096116 30.17299194643805 27.346971932495922 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 186.0 1 121.5 2 65.0 3 76.0 4 67.5 5 61.0 6 92.5 7 166.5 8 229.0 9 251.5 10 227.5 11 208.5 12 225.5 13 237.5 14 262.0 15 302.5 16 346.0 17 387.5 18 483.5 19 595.5 20 674.5 21 800.5 22 990.5 23 1245.5 24 1502.5 25 1826.0 26 2348.5 27 3017.5 28 3650.0 29 4397.0 30 5221.5 31 6012.0 32 6852.5 33 7668.5 34 8638.5 35 9591.0 36 10408.0 37 10860.5 38 10697.5 39 10524.0 40 10512.0 41 10357.5 42 10110.5 43 9896.0 44 9801.5 45 9784.0 46 9990.5 47 11057.0 48 13017.0 49 12071.5 50 9011.5 51 7278.5 52 5996.5 53 5061.5 54 4381.0 55 3574.5 56 2780.5 57 2248.0 58 1794.0 59 1365.0 60 1004.0 61 721.0 62 482.0 63 324.0 64 208.5 65 136.0 66 103.5 67 65.0 68 34.0 69 30.5 70 28.0 71 16.0 72 9.0 73 5.0 74 4.0 75 3.5 76 4.0 77 3.5 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.058557299261800234 2 0.0 3 0.0 4 0.0 5 0.017000506237296844 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001888945137477427 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0024556286787206553 24-25 5.666835412432281E-4 26-27 0.0026445231924683977 28-29 0.005477940898684539 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.013033721448594247 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.7778902749548545E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0015111561099819418 86-87 0.0 88-89 0.0 90-91 0.01038919825612585 92-93 9.444725687387135E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.012278143393603275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 264698.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.30843397411961 #Duplication Level Percentage of deduplicated Percentage of total 1 88.69355336232016 68.56759714040982 2 8.41546903940185 13.011734651876914 3 1.6779125236648367 3.8915036865027433 4 0.5025723972337078 1.5541233995502846 5 0.2309572827931632 0.8927472923828662 6 0.09934356528614649 0.46080572746066184 7 0.06443892067977819 0.34871704313153135 8 0.04481286977416997 0.27715302273017917 9 0.03140511230445363 0.21850920459347986 >10 0.1896941952341556 2.84272657338334 >50 0.025409000274466322 1.3565209811654761 >100 0.020522654067838153 3.331428162878952 >500 0.0019545384826512523 0.9727211157062368 >1k 0.0019545384826512523 2.2737119982275105 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2034 0.7684228819258174 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1747 0.6599974310346131 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1162 0.438990849949754 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1076 0.4065009935851423 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 826 0.31205373671127096 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 694 0.2621855850818669 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 546 0.20627280901253506 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 509 0.1922946149952021 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 431 0.16282707085055423 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 410 0.15489350127314902 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 396 0.14960445488821222 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 364 0.1375152060083567 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 351 0.1326039486509154 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 316 0.1193813326885734 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 311 0.11749238755109598 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 310 0.11711459852360047 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 302 0.1140922863036366 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 299 0.11295891922115013 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 294 0.11106997408367272 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 276 0.10426977158875397 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 275 0.10389198256125849 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 268 0.1012474593687901 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.8889451374774272E-4 0.0 20-21 0.0 0.0 0.0 5.666835412432282E-4 0.0 22-23 0.0 0.0 0.0 0.001322261596234199 0.0 24-25 0.0 0.0 0.0 0.00207783965122517 0.0 26-27 0.0 0.0 0.0 0.004533468329945825 0.0 28-29 0.0 0.0 0.0 0.008500253118648422 0.0 30-31 0.0 0.0 0.0 0.017000506237296844 0.0 32-33 0.0 0.0 0.0 0.028334177062161407 0.0 34-35 0.0 0.0 0.0 0.037778902749548544 0.0 36-37 0.0 0.0 0.0 0.04797920649192665 0.0 38-39 0.0 0.0 0.0 0.06705755238044867 0.0 40-41 0.0 0.0 0.0 0.1154145478998708 0.0 42-43 0.0 0.0 0.0 0.19267240402269759 0.0 44-45 0.0 0.0 0.0 0.26823020952179466 0.0 46-47 0.0 0.0 0.0 0.4486244701508889 0.0 48-49 0.0 0.0 0.0 0.6154183257901458 0.0 50-51 0.0 0.0 0.0 0.721010358975134 0.0 52-53 0.0 0.0 0.0 0.8526698350573106 0.0 54-55 0.0 0.0 0.0 0.9745067964246046 0.0 56-57 0.0 0.0 0.0 1.3252839084541628 0.0 58-59 0.0 0.0 0.0 1.7433074673779174 0.0 60-61 0.0 0.0 0.0 2.1596309756779424 0.0 62-63 0.0 0.0 0.0 2.645278770523389 0.0 64-65 0.0 0.0 0.0 3.2954536868431195 0.0 66-67 0.0 0.0 0.0 4.186846897218717 0.0 68-69 0.0 0.0 0.0 5.216133102630167 0.0 70-71 0.0 0.0 0.0 5.914476119955571 0.0 72-73 0.0 0.0 0.0 6.49041549237244 0.0 74-75 0.0 0.0 0.0 7.312862205230111 0.0 76-77 0.0 0.0 0.0 8.388805355537254 0.0 78-79 0.0 0.0 0.0 9.386168388125334 0.0 80-81 0.0 0.0 0.0 10.365586441907382 0.0 82-83 0.0 0.0 0.0 11.553355144353187 0.0 84-85 0.0 0.0 0.0 13.393754391797444 0.0 86-87 0.0 0.0 0.0 15.581152860996305 0.0 88-89 0.0 0.0 0.0 17.852042705271668 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTAGAC 60 8.265504E-5 39.589787 3 CATTCCG 75 6.9033467E-6 37.99902 9 CTAGACT 120 1.4962461E-7 31.671831 4 GAATAGC 60 0.0039460063 31.66585 6 TTTAGGC 135 4.1984276E-7 28.152739 3 CATATAG 70 0.00837344 27.147285 3 GGCATTC 145 7.850904E-7 26.206219 7 TTAGGCA 110 9.439154E-5 25.913317 4 TGTAGGT 95 0.0012228972 25.004078 2 GTACATG 900 0.0 24.814291 1 TACATGG 875 0.0 24.432556 2 TCTTAGG 140 1.7705373E-5 23.753876 2 GACCATC 100 0.001650544 23.749386 7 GAGTACT 1710 0.0 23.471615 12-13 CCTTTAG 185 2.5177542E-7 23.116243 1 TAGGCAT 165 2.4147284E-6 23.034058 5 GCATTCC 165 2.4186993E-6 23.029707 8 CATGGGG 440 0.0 22.674152 4 AGTACAT 660 0.0 22.314243 2 ACATGGG 915 0.0 21.806835 3 >>END_MODULE