FastQCFastQC Report
Thu 26 May 2016
SRR937260_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937260_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323142
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17300.5353683519938603No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17050.5276318151153363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.4505759078052373No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9210.2850140186048239No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.2580908702675604No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7510.23240556783086075No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC6200.19186611458739503No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5160.1596821211727352No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT5050.15627804494618466No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA4830.14946989249308354No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4660.1442090474156872No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4540.14049550971399571No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4320.1336873572608946No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4150.12842651218349826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4080.12626028185751154No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3710.11481020727729604No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3670.11357236137673221No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.1052169015479263No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCGG250.005017162357.002427
GCGTCTA250.001616183737.9957282-83
ACTAGGT650.005832278629.2365463
GTCCTAG1004.92511E-528.5144821
GGGTAAT700.00836201527.156651
GACGTGG700.00838103227.144017
GGACGTG1257.3669016E-626.601136
AGGACGT1309.964582E-625.5859495
TTTAGGC1151.2772427E-424.7875063
GTATAAG1050.002186350322.6305431
GTACATG11350.022.6106051
GTATAGC1754.016565E-621.725321
ATAGCCC1754.0328214E-621.7152088
AGTACAT8800.021.5951792
GGCATTC1100.00287221521.5918277
GAGTACT13900.021.52969212-13
TAGGACC1353.7827177E-421.1152844
TACATGG11650.020.7981012
GGGCTAT1604.9555674E-520.7918111
GCTATAC1604.967436E-520.7853573
Produced by FastQC (version 0.11.5)