##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937260_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 323142 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00788507838659 33.0 31.0 34.0 30.0 34.0 2 32.10292998124663 33.0 31.0 34.0 30.0 34.0 3 32.144007897456845 34.0 31.0 34.0 30.0 34.0 4 35.73955103329187 37.0 35.0 37.0 35.0 37.0 5 35.772267300443765 37.0 35.0 37.0 35.0 37.0 6 35.81577448923384 37.0 35.0 37.0 35.0 37.0 7 35.71830031379393 37.0 35.0 37.0 35.0 37.0 8 35.738972340333355 37.0 35.0 37.0 35.0 37.0 9 37.40874290559568 39.0 37.0 39.0 35.0 39.0 10-11 37.32438525477963 39.0 37.0 39.0 34.5 39.0 12-13 37.21645747070947 39.0 37.0 39.0 34.0 39.0 14-15 38.45369992139678 40.0 38.0 41.0 34.0 41.0 16-17 38.41529884694654 40.0 38.0 41.0 34.0 41.0 18-19 38.43454735070031 40.0 38.0 41.0 34.0 41.0 20-21 38.35664661356307 40.0 38.0 41.0 34.0 41.0 22-23 38.228405778264666 40.0 38.0 41.0 34.0 41.0 24-25 38.178463338099036 40.0 38.0 41.0 34.0 41.0 26-27 38.16983709947948 40.0 38.0 41.0 34.0 41.0 28-29 38.112752288467604 40.0 38.0 41.0 34.0 41.0 30-31 37.92814923470177 40.0 37.5 41.0 33.5 41.0 32-33 37.696361042513814 40.0 37.0 41.0 33.0 41.0 34-35 37.714665688768406 40.0 37.0 41.0 33.0 41.0 36-37 37.60314350966448 40.0 37.0 41.0 33.0 41.0 38-39 37.48327979649813 40.0 37.0 41.0 33.0 41.0 40-41 37.28281529482395 40.0 37.0 41.0 32.5 41.0 42-43 37.013890178311705 40.0 37.0 41.0 31.5 41.0 44-45 36.74547876784819 40.0 36.5 41.0 31.0 41.0 46-47 36.35431482134789 40.0 36.0 41.0 30.0 41.0 48-49 35.99954973355367 39.5 36.0 41.0 29.5 41.0 50-51 34.99798076387471 38.5 35.0 40.0 27.0 40.5 52-53 35.0960351795805 38.5 35.0 40.0 27.5 40.5 54-55 35.493812317804554 39.0 36.0 40.5 27.5 41.0 56-57 35.30972142278008 39.0 35.0 41.0 26.5 41.0 58-59 35.08782052472288 39.0 35.0 41.0 26.0 41.0 60-61 34.81262262410952 39.0 35.0 40.0 24.5 41.0 62-63 34.559839018140636 38.5 35.0 40.0 23.0 41.0 64-65 34.17465696195481 38.0 34.0 40.0 22.0 41.0 66-67 33.85091229242872 38.0 34.0 40.0 21.5 41.0 68-69 33.42015739210626 37.0 34.0 40.0 19.0 41.0 70-71 32.96557550550531 36.5 33.0 39.0 17.0 41.0 72-73 32.521376051395364 36.0 32.5 39.0 12.5 40.0 74-75 32.055768052435155 35.5 32.0 38.5 8.0 40.0 76-77 31.57329749769451 35.0 31.5 37.5 7.0 39.5 78-79 31.082329130846503 35.0 31.0 37.0 2.0 39.0 80-81 30.586104560843218 35.0 31.0 36.5 2.0 39.0 82-83 30.10989750635943 34.5 30.5 36.0 2.0 37.5 84-85 29.58085299961008 34.0 30.0 35.5 2.0 37.0 86-87 29.159774959615277 34.0 29.0 35.0 2.0 37.0 88-89 28.690119204560226 34.0 29.0 35.0 2.0 36.0 90-91 28.225052763181512 34.0 29.0 35.0 2.0 36.0 92-93 27.804520922690337 34.0 28.0 35.0 2.0 35.5 94-95 27.32644317358932 34.0 26.5 35.0 2.0 35.0 96-97 26.67037710975361 33.0 25.5 35.0 2.0 35.0 98-99 25.884139171014603 33.0 24.0 35.0 2.0 35.0 100-101 23.48799289476453 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.026295400995657303 1101 2 0.10696875009080031 1101 3 -0.027462823807567815 1101 4 -0.05414701671799804 1101 5 -0.018486389262449165 1101 6 0.007217972931080396 1101 7 -0.06250705536798051 1101 8 -0.05123697789338877 1101 9 -0.04457478181155494 1101 10-11 -0.1353003226476659 1101 12-13 -0.08933033923961631 1101 14-15 0.06983971605886552 1101 16-17 -0.09878658616158731 1101 18-19 -0.01082988400305851 1101 20-21 0.04029618711248162 1101 22-23 0.044604648518067336 1101 24-25 -0.05007511578175894 1101 26-27 -0.05359061830220213 1101 28-29 0.06580217185758386 1101 30-31 0.08575496475801003 1101 32-33 -0.12720300514598648 1101 34-35 0.20845865427243382 1101 36-37 0.1852615309631318 1101 38-39 0.25095241182783923 1101 40-41 0.1800317903741231 1101 42-43 0.38474245960961895 1101 44-45 0.4382931381229582 1101 46-47 0.26903808271832474 1101 48-49 0.2557861613392447 1101 50-51 0.49170030469143455 1101 52-53 0.5739241785954903 1101 54-55 0.6508469308509035 1101 56-57 0.48771533696421443 1101 58-59 0.41850861183831256 1101 60-61 0.21958527231942782 1101 62-63 0.2649754253300145 1101 64-65 0.4033736887435495 1101 66-67 0.35798364458118925 1101 68-69 0.2831925900795298 1101 70-71 0.5526873064121602 1101 72-73 0.5670903376689225 1101 74-75 0.31518808619476246 1101 76-77 0.34859202116028243 1101 78-79 0.40813643282983136 1101 80-81 0.3294582433973119 1101 82-83 0.3183875454609222 1101 84-85 0.5298738019262821 1101 86-87 0.5998059773195159 1101 88-89 0.15485634409023774 1101 90-91 0.19601866129072576 1101 92-93 0.5783079272783702 1101 94-95 0.6953938894753087 1101 96-97 0.7240260837862209 1101 98-99 0.9967761169494374 1101 100-101 1.1757181427823262 1102 1 -0.3016590067511906 1102 2 -0.5259328071889016 1102 3 -0.3192390994338652 1102 4 -0.4180154637671549 1102 5 -0.32793699400791354 1102 6 -0.33327092927737567 1102 7 -0.34107105775070323 1102 8 -0.42419603131596517 1102 9 -0.2813967324625537 1102 10-11 -0.4153054284827462 1102 12-13 -0.2419023111649139 1102 14-15 -0.23672498190138924 1102 16-17 -0.3146838626795514 1102 18-19 -0.3833042530809365 1102 20-21 -0.48116496969215206 1102 22-23 -0.3253427021825033 1102 24-25 -0.4135199265143754 1102 26-27 -0.6449277181138839 1102 28-29 -0.4720180065506554 1102 30-31 -0.5390808530450713 1102 32-33 -0.5883283607970071 1102 34-35 -0.5248410101484069 1102 36-37 -0.48648507162160115 1102 38-39 -0.5491818938340742 1102 40-41 -0.40408869253428037 1102 42-43 -0.42184813751408967 1102 44-45 -0.21732568538904218 1102 46-47 0.3127188718288352 1102 48-49 -0.3106155938840658 1102 50-51 -0.32696855230449273 1102 52-53 -0.04545986529988966 1102 54-55 -0.03668336192063748 1102 56-57 0.1534200347024779 1102 58-59 0.1110522426790368 1102 60-61 0.02750782642092986 1102 62-63 0.07114608601767713 1102 64-65 0.193760659244397 1102 66-67 0.02223983910609917 1102 68-69 -0.31131345737513527 1102 70-71 -0.15048053126668037 1102 72-73 0.05469018819375293 1102 74-75 0.23878370405630633 1102 76-77 -0.39379454975347983 1102 78-79 -0.2283447588276779 1102 80-81 0.05141222428373027 1102 82-83 -0.12600695059685663 1102 84-85 0.07307730645308297 1102 86-87 0.2638420289550467 1102 88-89 -0.3268847456725652 1102 90-91 -0.4588250372578102 1102 92-93 -0.39524902683200835 1102 94-95 -0.014278338111740396 1102 96-97 0.35757142027185296 1102 98-99 0.2383764632582519 1102 100-101 0.4386298007357752 1103 1 0.06712052064223784 1103 2 0.06458981978677514 1103 3 -0.10833725974315911 1103 4 0.27781925434746313 1103 5 0.08637414215780126 1103 6 0.05532324075834083 1103 7 0.19732349343291844 1103 8 0.150289776604005 1103 9 0.25241647140951073 1103 10-11 0.1571421216112796 1103 12-13 0.19184694736357955 1103 14-15 0.2076972592987687 1103 16-17 0.12117937842383952 1103 18-19 0.06832866699159013 1103 20-21 -0.026193850270011865 1103 22-23 0.01478131812856276 1103 24-25 0.03299105567768379 1103 26-27 0.08847699280688204 1103 28-29 0.09237116817578794 1103 30-31 -0.06225472968945667 1103 32-33 -0.0912500740273785 1103 34-35 0.19687895131610844 1103 36-37 0.123959474724046 1103 38-39 0.026971962028063956 1103 40-41 -0.004541778948841113 1103 42-43 0.0074312573610271215 1103 44-45 -0.08024285513448604 1103 46-47 0.08653325364143427 1103 48-49 0.19367757868843682 1103 50-51 -0.02260344122048963 1103 52-53 0.143025345235948 1103 54-55 0.16418284506875125 1103 56-57 0.43067778912712384 1103 58-59 0.4600991625748705 1103 60-61 0.43283085557916223 1103 62-63 0.3170306562586944 1103 64-65 0.14676924558954596 1103 66-67 0.011848720777770438 1103 68-69 0.03308270085592824 1103 70-71 0.09800602548691018 1103 72-73 0.043346200107770017 1103 74-75 0.03380311904055233 1103 76-77 -0.003557573358566657 1103 78-79 0.1438760377125412 1103 80-81 0.2524067426532923 1103 82-83 0.054848991482128184 1103 84-85 -0.09078053295155186 1103 86-87 -0.04068084202259925 1103 88-89 -2.1448909187427034E-4 1103 90-91 -0.0980428847397512 1103 92-93 0.0484664500445966 1103 94-95 -0.15094468224706858 1103 96-97 -0.6967085004695761 1103 98-99 -0.42074647587167036 1103 100-101 0.25627198446925803 1104 1 0.17762320077043725 1104 2 0.24284262594343886 1104 3 0.2245536250460134 1104 4 0.23681953646622134 1104 5 0.32053733629713577 1104 6 0.23115260053375408 1104 7 0.16411210323492753 1104 8 0.11910871648495913 1104 9 0.1261613898031868 1104 10-11 0.2638223238118016 1104 12-13 0.08828392619542313 1104 14-15 0.3006646398954729 1104 16-17 0.1753508048810346 1104 18-19 0.43732612792276626 1104 20-21 0.38804667215842414 1104 22-23 0.35693356051019265 1104 24-25 0.41482260765459245 1104 26-27 0.17040705510425624 1104 28-29 0.2960169750123143 1104 30-31 0.1524330203440769 1104 32-33 0.2095856449823401 1104 34-35 0.4620451470140239 1104 36-37 0.4004104207468657 1104 38-39 0.6245781149919338 1104 40-41 0.7206190518677786 1104 42-43 1.1285365154071911 1104 44-45 0.9974646755864001 1104 46-47 0.9053898864384351 1104 48-49 0.6277381000993998 1104 50-51 0.764843199022593 1104 52-53 0.5720470406309346 1104 54-55 0.6682435052266413 1104 56-57 0.568058062088582 1104 58-59 0.5785636040491013 1104 60-61 0.43083752468161407 1104 62-63 0.6445335352574943 1104 64-65 0.9782680361788039 1104 66-67 1.0141033583939105 1104 68-69 0.950967642651591 1104 70-71 0.8731226885307422 1104 72-73 1.1247975854958767 1104 74-75 0.8660904546832562 1104 76-77 0.6265731724986807 1104 78-79 0.8748665520474113 1104 80-81 0.712461501441755 1104 82-83 0.5848923267733319 1104 84-85 0.6082519384550196 1104 86-87 0.844025083858785 1104 88-89 0.8561107522121638 1104 90-91 0.741232671288568 1104 92-93 0.6489192275268927 1104 94-95 1.0385928742344959 1104 96-97 0.8284116312475085 1104 98-99 1.397057992704461 1104 100-101 1.4863305819227897 1105 1 -0.1394183627337462 1105 2 -0.024270053822661453 1105 3 -0.008749857016880469 1105 4 -0.004512181386594705 1105 5 -0.10947899410375328 1105 6 -0.016437348367894344 1105 7 -0.1645556521251308 1105 8 -0.1502586121775238 1105 9 -0.08599685704925975 1105 10-11 -0.19657908797769608 1105 12-13 -0.14939188900765288 1105 14-15 -0.09535021550146894 1105 16-17 -0.29062664863000265 1105 18-19 -0.2236152406701848 1105 20-21 -0.17880261404501852 1105 22-23 -0.030194396082542596 1105 24-25 -0.08718181735725494 1105 26-27 -0.09858625469924931 1105 28-29 -0.21926511949329353 1105 30-31 -0.47455691802183253 1105 32-33 -0.37517967135333663 1105 34-35 -0.015010639742541798 1105 36-37 -0.2660911859538686 1105 38-39 -0.07886707280768235 1105 40-41 -0.02849331182605397 1105 42-43 0.1291317848588065 1105 44-45 0.04950070782577143 1105 46-47 -0.29978052919660314 1105 48-49 -0.1219985063379454 1105 50-51 -0.13357643960144117 1105 52-53 -0.21196760405114645 1105 54-55 -0.11071661698796476 1105 56-57 0.05878924261853058 1105 58-59 0.30210835461539887 1105 60-61 0.43307110209297406 1105 62-63 0.2549138755619751 1105 64-65 0.12700374707802808 1105 66-67 -0.010710949144417725 1105 68-69 -0.0047822388213063505 1105 70-71 -0.09674772183642233 1105 72-73 0.25986053655734764 1105 74-75 0.25264157937376197 1105 76-77 0.1655159107381614 1105 78-79 0.11648010101227513 1105 80-81 -0.07759012369652751 1105 82-83 -0.8007158489628523 1105 84-85 -0.3080573909449704 1105 86-87 -0.17222103108616338 1105 88-89 0.018553464112287088 1105 90-91 0.3826407732745558 1105 92-93 0.20276477354174816 1105 94-95 0.18325388671934917 1105 96-97 0.553563082546038 1105 98-99 0.6555814083921803 1105 100-101 0.6239405487351988 1106 1 -0.46947751073667376 1106 2 -0.3651752176426939 1106 3 -0.4102119424824231 1106 4 -0.3695151720159515 1106 5 -0.3783698029028457 1106 6 -0.47267447204703217 1106 7 -0.8474226688061961 1106 8 -0.8494943402296329 1106 9 -0.879470639198523 1106 10-11 -1.3147288852794787 1106 12-13 -0.9368777665912233 1106 14-15 -1.0372688703828317 1106 16-17 -0.6253212721429904 1106 18-19 -0.9435361551381902 1106 20-21 -0.9953108881195831 1106 22-23 -0.726602317927437 1106 24-25 -0.737580567938771 1106 26-27 -0.7920666826915408 1106 28-29 -0.41525767614911047 1106 30-31 -0.30064517481720543 1106 32-33 -0.5553551216091961 1106 34-35 -0.1687921244238737 1106 36-37 -0.40623207782600446 1106 38-39 -0.6002999165639125 1106 40-41 -0.6210632593238969 1106 42-43 -0.5340037238804811 1106 44-45 -0.3105843589649524 1106 46-47 -0.6402636819845355 1106 48-49 -0.36286047217831197 1106 50-51 -0.3395111774229349 1106 52-53 -0.4443694117204515 1106 54-55 -0.8194625464091132 1106 56-57 -0.7936364900856603 1106 58-59 -0.7736177037351055 1106 60-61 -1.1409370058355108 1106 62-63 -1.2555965251823622 1106 64-65 -1.117267536234266 1106 66-67 -0.7712645478684124 1106 68-69 -1.1426170231636945 1106 70-71 -0.6541046700653084 1106 72-73 -0.9176898057035636 1106 74-75 -0.5282516219288205 1106 76-77 -0.04507424012247796 1106 78-79 -0.12529234532254563 1106 80-81 -0.5782912601356855 1106 82-83 -0.7687161147965718 1106 84-85 -0.824293185455506 1106 86-87 -0.6960729617035604 1106 88-89 -0.2605287449659386 1106 90-91 -0.12497868575382043 1106 92-93 0.2057986008827477 1106 94-95 0.2792929642763369 1106 96-97 0.5165490598172546 1106 98-99 0.5222691202284295 1106 100-101 1.069428021321098 1107 1 0.005967459545527021 1107 2 -0.015540913899684483 1107 3 0.26597557444795683 1107 4 0.033770517229868346 1107 5 0.05654582403916919 1107 6 0.13289940815756296 1107 7 0.04007992054638265 1107 8 0.008035497079355025 1107 9 0.14568193342285696 1107 10-11 -0.1333427450879512 1107 12-13 -0.05245849862111385 1107 14-15 -0.09640569974051516 1107 16-17 -0.19448487142275184 1107 18-19 -0.008624511815526148 1107 20-21 -0.11854016931530964 1107 22-23 0.18478206947158782 1107 24-25 0.3623047038237459 1107 26-27 0.30244070293874614 1107 28-29 0.3456614245089824 1107 30-31 -0.16089980776651913 1107 32-33 -0.043101508466435234 1107 34-35 -0.19274562761873426 1107 36-37 -0.2926351882359981 1107 38-39 -0.15617416166804077 1107 40-41 0.2949341592907615 1107 42-43 0.22414676132435574 1107 44-45 0.0844671890695281 1107 46-47 0.1035856097279435 1107 48-49 0.1894763189366273 1107 50-51 -0.13227134760315096 1107 52-53 0.1380038693477985 1107 54-55 -0.1508678079735546 1107 56-57 -0.232053718442657 1107 58-59 0.1126061438038164 1107 60-61 0.29221387773126395 1107 62-63 0.09424492676722451 1107 64-65 0.21145674950278703 1107 66-67 0.0630089196332193 1107 68-69 -0.22964317164115755 1107 70-71 0.137755202140184 1107 72-73 0.1657869379266259 1107 74-75 0.04011126986014446 1107 76-77 0.36801911998077586 1107 78-79 0.3818702022817071 1107 80-81 0.1523842023625228 1107 82-83 -0.01915829654522483 1107 84-85 0.08319920583152296 1107 86-87 0.1803392313585661 1107 88-89 0.3589399995565863 1107 90-91 0.261659458198249 1107 92-93 0.0394785091001566 1107 94-95 0.3355217515032578 1107 96-97 0.4928584754999683 1107 98-99 0.7626845320549975 1107 100-101 0.8192407912113104 1108 1 -0.046513032464638115 1108 2 -0.10768617065860298 1108 3 -0.15869425615692023 1108 4 -0.07296796016930074 1108 5 -0.0050976463389034166 1108 6 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-1.0303756052445152 2316 56-57 -1.0289591765323252 2316 58-59 -0.4743821602897498 2316 60-61 -0.9290336856215688 2316 62-63 -0.71411277303784 2316 64-65 -0.6223639132652394 2316 66-67 -0.721347805512977 2316 68-69 -0.6102474601401866 2316 70-71 -0.8648032773919851 2316 72-73 -0.5628923993053405 2316 74-75 -0.44003005567591913 2316 76-77 -0.44119140633501175 2316 78-79 -0.16272044099314442 2316 80-81 -0.38319084637042167 2316 82-83 -0.2930374388454169 2316 84-85 -0.2954557649287075 2316 86-87 -0.10048435538607592 2316 88-89 -0.7494907996636861 2316 90-91 -0.7812255320551635 2316 92-93 -0.3219980990869793 2316 94-95 0.215638710567589 2316 96-97 -0.11362300598151265 2316 98-99 0.08273813829281451 2316 100-101 0.3978226625563366 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1015.0 3 194.0 4 111.0 5 108.0 6 122.0 7 157.0 8 203.0 9 313.0 10 379.0 11 534.0 12 888.0 13 1289.0 14 1532.0 15 2287.0 16 3046.0 17 4237.0 18 3507.0 19 2196.0 20 2029.0 21 2055.0 22 2017.0 23 2177.0 24 2495.0 25 2714.0 26 2996.0 27 3440.0 28 4012.0 29 5135.0 30 6378.0 31 7937.0 32 10282.0 33 13538.0 34 19127.0 35 27120.0 36 40993.0 37 66403.0 38 69531.0 39 10641.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.657777226494666 21.67731332175639 15.362503100967501 21.302406350781446 2 14.579399846845925 22.85189008869915 40.186823169049234 22.381886895405685 3 18.95797037652442 26.069340508769862 31.625757171819878 23.34693194288584 4 11.638015222210166 18.929174869402118 39.86358915533785 29.56922075304987 5 11.83072043947459 39.45908911946975 35.83100565153906 12.879184789516597 6 28.395207204078343 39.42521179345332 18.27439500874462 13.90518599372372 7 25.324675324675322 35.19424701373126 22.26925414604498 17.211823515548446 8 23.641674237467868 35.75722998595397 21.409812378492514 19.191283398085645 9 24.186972334270717 18.22688812478878 23.033897727026098 34.55224181391441 10-11 22.659023886530452 28.419172483510756 29.284799508811314 19.637004121147474 12-13 24.37415305613009 26.492559297699415 28.90664003994009 20.226647606230404 14-15 21.036948840901236 28.880186410801407 28.392609921755213 21.690254826542144 16-17 19.114051421669785 31.6432577083666 29.325980145151565 19.91671072481205 18-19 18.809640085889477 30.85385611187337 31.59042812957838 18.746075672658773 20-21 20.368156936428285 29.039874366522955 31.326825320788586 19.26514337626017 22-23 19.54400928910158 29.58560251264617 31.051674503183442 19.81871369506881 24-25 19.870498087854816 29.487185449404503 31.36088620604663 19.28143025669406 26-27 19.794591158893425 29.382765810667305 31.778530512901998 19.044112517537265 28-29 18.986217267949336 30.645571386490133 31.280600300770526 19.087611044790005 30-31 19.817527866920916 29.72280359053067 31.1301799916283 19.32948855092011 32-33 19.358229410196145 29.85420787934787 31.18546791767179 19.60209479278419 34-35 20.069831839225525 29.924276486949942 30.83267956426421 19.173212109560325 36-37 19.66670542185017 29.723520520869666 31.273417819633377 19.336356237646786 38-39 19.428670644609703 30.306158990600828 31.250571654908015 19.014598709881454 40-41 19.885159572076784 30.22901426048996 30.701056786173265 19.18476938125999 42-43 19.374601781846987 30.330929943059648 30.237450411666494 20.05701786342687 44-45 19.94272841579108 30.029752076366243 30.11223308015386 19.915286427688812 46-47 20.20040558642588 30.15990896011113 29.82440092280214 19.815284530660847 48-49 20.01972187810577 29.971673057154685 30.109509139198938 19.899095925540607 50-51 19.551118560081616 30.470199897361315 30.107863642494898 19.870817900062175 52-53 19.931305581615327 30.757421865914075 29.280724470254388 20.03054808221621 54-55 19.72184752779199 30.0234119416407 29.61889700296138 20.635843527605935 56-57 20.219483118650793 30.200709700322914 29.250722024738746 20.329085156287544 58-59 20.656114804590075 30.01746932018458 29.08324045813951 20.243175417085833 60-61 20.006324247910516 29.81479874011061 30.055678182584757 20.123198829394113 62-63 19.92091222217744 29.590795931211307 30.083118123223162 20.405173723388096 64-65 19.68094093284166 29.91798768342719 29.81599542429865 20.5850759594325 66-67 19.72728695940615 29.329594650521507 29.789799844066543 21.153318546005796 68-69 19.57198727060505 29.283808773857928 29.984762548421152 21.159441407115875 70-71 20.015718054681468 29.05049928850226 29.816405680821184 21.11737697599509 72-73 19.497510494366836 28.7475895508969 30.533647079248745 21.221252875487515 74-75 19.354018626685022 29.806726440715803 29.80750151915374 21.031753413445443 76-77 19.482033482104793 29.13383385447268 29.761061356987945 21.623071306434586 78-79 19.289689491062987 29.129086765777355 29.7966112204877 21.78461252267196 80-81 19.606046795700525 29.125781659334493 29.590669382516253 21.67750216244873 82-83 19.554430505327023 28.828705935604482 29.91317719386086 21.703686365207634 84-85 19.575421245989055 28.80206476414875 29.78592798127451 21.836586008587684 86-87 19.688997885520465 28.512454346464605 29.62100589697958 22.17754187103535 88-89 19.81875706284752 27.952522163196154 29.394466912933016 22.834253861023313 90-91 19.591948730827543 27.565241386152735 30.006076897557772 22.836732985461953 92-93 19.79067038491289 27.863439708451466 30.214987591205798 22.130902315429843 94-95 19.465271975607372 28.56336796809241 29.71913447721562 22.252225579084595 96-97 19.330359980343008 29.173894642848836 29.221951964381777 22.273793412426382 98-99 20.155364428955885 29.029212605260874 28.27424875671251 22.54117420907073 100-101 20.36655268729869 28.26507488227471 28.8091377765653 22.559234653861296 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 498.0 1 297.5 2 91.5 3 92.5 4 86.0 5 68.5 6 98.0 7 176.5 8 262.0 9 325.5 10 293.5 11 230.0 12 237.5 13 239.5 14 258.0 15 323.5 16 377.0 17 423.0 18 528.5 19 644.0 20 754.0 21 920.5 22 1112.5 23 1334.5 24 1597.5 25 2001.0 26 2644.0 27 3581.5 28 4479.0 29 5383.0 30 6435.5 31 7449.5 32 8538.5 33 9502.0 34 10559.5 35 11718.5 36 12844.0 37 13537.0 38 13169.0 39 12745.5 40 12757.5 41 12667.0 42 12440.0 43 12241.5 44 12053.5 45 12060.0 46 12295.0 47 13287.0 48 15723.0 49 14766.5 50 10961.0 51 8715.0 52 7162.5 53 6118.0 54 5278.0 55 4459.0 56 3609.0 57 2862.5 58 2271.5 59 1736.5 60 1300.5 61 921.5 62 596.0 63 398.0 64 270.5 65 178.5 66 119.0 67 84.0 68 61.5 69 39.0 70 27.5 71 21.5 72 15.0 73 10.0 74 7.0 75 6.0 76 5.0 77 4.0 78 2.0 79 0.5 80 0.5 81 1.0 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20486349654331532 2 0.18289173180830717 3 0.17329842607893745 4 0.18165388590774334 5 0.17794034820605184 6 0.20362565064275145 7 0.20610134244387915 8 0.1952701908139456 9 0.1896998842614083 10-11 0.20393511211789245 12-13 0.20393511211789245 14-15 0.1944965371260932 16-17 0.20161415105433522 18-19 0.19635330597693895 20-21 0.19078299942440166 22-23 0.1898546149989788 24-25 0.18521269287186437 26-27 0.19016407647411976 28-29 0.19712695966479132 30-31 0.19403234491338173 32-33 0.1943418063885227 34-35 0.20207834326704666 36-37 0.18706946172271013 38-39 0.1898546149989788 40-41 0.18799784614813303 42-43 0.18923569204869686 44-45 0.19913845925320756 46-47 0.19991211294105998 48-49 0.203470919905181 50-51 0.20161415105433522 52-53 0.2164683018611013 54-55 0.20331618916761052 56-57 0.18923569204869686 58-59 0.17840454041876327 60-61 0.17763088673091088 62-63 0.18227280885802527 64-65 0.17592884861763558 66-67 0.17592884861763558 68-69 0.17995184779446807 70-71 0.1804160400071795 72-73 0.1819633473828843 74-75 0.18382011623373004 76-77 0.18923569204869686 78-79 0.18784311541056253 80-81 0.18289173180830717 82-83 0.18211807812045477 84-85 0.18165388590774334 86-87 0.18691473098513967 88-89 0.18304646254587764 90-91 0.18846203836084446 92-93 0.18211807812045477 94-95 0.1811896936950319 96-97 0.1889262305735559 98-99 0.18877149983598543 100-101 0.17608357935520608 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 323142.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.4925710815172 #Duplication Level Percentage of deduplicated Percentage of total 1 87.90520463366143 68.12000318509324 2 9.019254695639454 13.978504712082962 3 1.8491698373200784 4.298907751809714 4 0.5437940821180949 1.6856000624897949 5 0.22158903094392068 0.8585751865653144 6 0.10161228748973383 0.4724518446632257 7 0.05993707427264896 0.3251274591943997 8 0.04752461397147608 0.29462436210450205 9 0.031640433572523594 0.22067086929019275 >10 0.17521214904823162 2.677671032318161 >50 0.022729966653771458 1.1659255471972128 >100 0.018742253205477653 3.109031786435459 >500 0.0023926280687843813 1.2814983419488966 >1k 0.0011963140343921907 1.5114078588068611 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1730 0.5353683519938603 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1705 0.5276318151153363 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1456 0.4505759078052373 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 921 0.2850140186048239 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 834 0.2580908702675604 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 751 0.23240556783086075 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 620 0.19186611458739503 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 516 0.1596821211727352 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 505 0.15627804494618466 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 483 0.14946989249308354 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 466 0.1442090474156872 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 454 0.14049550971399571 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 432 0.1336873572608946 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 415 0.12842651218349826 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 408 0.12626028185751154 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 371 0.11481020727729604 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 367 0.11357236137673221 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 340 0.1052169015479263 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.094614751409597E-4 0.0 22-23 0.0 0.0 0.0 0.0012378459005638388 0.0 24-25 0.0 0.0 0.0 0.0018567688508457582 0.0 26-27 0.0 0.0 0.0 0.0026304225386981576 0.0 28-29 0.0 0.0 0.0 0.007891267616094471 0.0 30-31 0.0 0.0 0.0 0.017948765558175664 0.0 32-33 0.0 0.0 0.0 0.025994763911840614 0.0 34-35 0.0 0.0 0.0 0.03667118480420373 0.0 36-37 0.0 0.0 0.0 0.05059695118554691 0.0 38-39 0.0 0.0 0.0 0.07473494624654177 0.0 40-41 0.0 0.0 0.0 0.11481020727729604 0.0 42-43 0.0 0.0 0.0 0.18536742360943487 0.0 44-45 0.0 0.0 0.0 0.2631969846073862 0.0 46-47 0.0 0.0 0.0 0.43046091192107494 0.0 48-49 0.0 0.0 0.0 0.5998910695607504 0.0 50-51 0.0 0.0 0.0 0.7190337374900199 0.0 52-53 0.0 0.0 0.0 0.8661826689195462 0.0 54-55 0.0 0.0 0.0 0.9921334893019169 0.0 56-57 0.0 0.0 0.0 1.32201942180218 0.0 58-59 0.0 0.0 0.0 1.6921353460707675 0.0 60-61 0.0 0.0 0.0 2.0452308892066027 0.0 62-63 0.0 0.0 0.0 2.4475308068898505 0.0 64-65 0.0 0.0 0.0 3.020498728113337 0.0 66-67 0.0 0.0 0.0 3.7936882237530254 0.0 68-69 0.0 0.0 0.0 4.676272350855042 0.0 70-71 0.0 0.0 0.0 5.309739990468587 0.0 72-73 0.0 0.0 0.0 5.841394804760755 0.0 74-75 0.0 0.0 0.0 6.546193314394291 0.0 76-77 0.0 0.0 0.0 7.495466389389184 0.0 78-79 0.0 0.0 0.0 8.374801171002222 0.0 80-81 0.0 0.0 0.0 9.233402033780814 0.0 82-83 0.0 0.0 0.0 10.262516169362076 0.0 84-85 0.0 0.0 0.0 11.790172741395423 0.0 86-87 0.0 0.0 0.0 13.605164293097152 0.0 88-89 0.0 0.0 0.0 15.476477833274537 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTCGG 25 0.0050171623 57.00242 7 GCGTCTA 25 0.0016161837 37.99572 82-83 ACTAGGT 65 0.0058322786 29.236546 3 GTCCTAG 100 4.92511E-5 28.514482 1 GGGTAAT 70 0.008362015 27.15665 1 GACGTGG 70 0.008381032 27.14401 7 GGACGTG 125 7.3669016E-6 26.60113 6 AGGACGT 130 9.964582E-6 25.585949 5 TTTAGGC 115 1.2772427E-4 24.787506 3 GTATAAG 105 0.0021863503 22.630543 1 GTACATG 1135 0.0 22.610605 1 GTATAGC 175 4.016565E-6 21.72532 1 ATAGCCC 175 4.0328214E-6 21.715208 8 AGTACAT 880 0.0 21.595179 2 GGCATTC 110 0.002872215 21.591827 7 GAGTACT 1390 0.0 21.529692 12-13 TAGGACC 135 3.7827177E-4 21.115284 4 TACATGG 1165 0.0 20.798101 2 GGGCTAT 160 4.9555674E-5 20.791811 1 GCTATAC 160 4.967436E-5 20.785357 3 >>END_MODULE