FastQCFastQC Report
Thu 26 May 2016
SRR937260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323142
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21120.6535826354977069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18700.5786929585135946No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14010.4335555266724846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12260.3793997685228166No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10190.31534124316863793No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9060.2803720964777095No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT6810.21074326457099354No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC6190.19155665311225406No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4790.1482320465925197No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.14451850889082818No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4390.13585358758688132No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4240.13121166545976692No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4120.1274981277580754No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4110.12718866628293443No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA4060.12564135890722963No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA4020.1244035130066658No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3700.1145007458021551No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3410.10552636302306727No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA3290.10181282532137573No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3270.10119390237109382No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG702.0504315E-433.9275749
GTGTACC804.4832763E-429.6912231
TTAGCCC804.487343E-429.686634
GTGTAGC2500.028.5035761
CCACGTC856.398234E-427.9403579
TAGGACC1056.892111E-527.1420614
GTAGGAC1309.994286E-625.5761743
GTATAGT1401.7726274E-523.752981
GTAGCTC1000.001651364723.7493023
GAGTACT18500.022.97905512-13
GTCCTAC2303.596142E-922.7202421
GGTCCAT1100.002873428221.5902756
TACAGCC1100.002873428221.5902754
GTATATG1553.89148E-521.4543061
GTACTTT19750.021.40443614-15
CATGGGG3401.8189894E-1220.9552674
GTACATG9750.020.9513451
TAGAGTG1856.529792E-620.5399385
TACATGG9100.020.3565462
TAGCCCT3052.0736479E-1020.2453084
Produced by FastQC (version 0.11.5)