FastQCFastQC Report
Thu 26 May 2016
SRR937259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814577
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42980.5276358158897194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34880.4281977026112939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.27020158929112903No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18290.224533715044741No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11410.14007270030948576No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9140.11220547597096406No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGA550.002574200734.559081
GTACATG39000.028.1457141
TACATGG40350.026.2586732
TACCTGG6950.025.294632
CATGGGG15050.025.2560214
ACATGGG39950.025.092873
GAGTACT36100.023.22712712-13
GTACCTG8000.023.1653821
GTATCAA68450.023.04781
GTGTAAG3600.022.4394021
GTGTAGG3450.022.0376741
GTACTTT38050.021.72063314-15
GGTATCA53100.020.7614821
ATCAACG78800.019.8963053
CAACGCA78800.019.7769375
TCAACGC79300.019.7720724
TATCAAC79250.019.7245922
AACGCAG80750.019.355816
AGTACTT37600.019.26814812-13
CATGGGA27700.019.0394614
Produced by FastQC (version 0.11.5)