##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937259_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 814577 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76867503010765 33.0 31.0 34.0 30.0 34.0 2 31.852093786100024 33.0 31.0 34.0 30.0 34.0 3 31.883753162684435 33.0 31.0 34.0 30.0 34.0 4 35.50719698690241 37.0 35.0 37.0 33.0 37.0 5 35.52924155727451 37.0 35.0 37.0 33.0 37.0 6 35.578546902257244 37.0 35.0 37.0 33.0 37.0 7 35.51884597772832 37.0 35.0 37.0 33.0 37.0 8 35.50752108149383 37.0 35.0 37.0 33.0 37.0 9 37.11320845052094 39.0 37.0 39.0 34.0 39.0 10-11 37.000699749686035 39.0 37.0 39.0 33.0 39.0 12-13 36.858096288012064 39.0 37.0 39.0 33.0 39.0 14-15 37.96003017517067 40.0 38.0 41.0 33.0 41.0 16-17 37.890580018831855 40.0 38.0 41.0 33.0 41.0 18-19 37.83962289630078 40.0 38.0 41.0 33.0 41.0 20-21 37.716296924661506 40.0 37.5 41.0 32.5 41.0 22-23 37.789426291191624 40.0 38.0 41.0 33.0 41.0 24-25 37.76123742752373 40.0 38.0 41.0 33.0 41.0 26-27 37.67978840551599 40.0 37.0 41.0 32.5 41.0 28-29 37.539667213780895 40.0 37.0 41.0 32.0 41.0 30-31 37.297320572518004 39.5 37.0 41.0 32.0 41.0 32-33 37.04900641682738 39.0 36.0 41.0 31.0 41.0 34-35 37.036189335078205 39.0 36.0 41.0 31.0 41.0 36-37 36.89129634153677 39.0 36.0 41.0 31.0 41.0 38-39 36.74096064583213 39.0 36.0 41.0 30.5 41.0 40-41 36.515692193617056 39.0 35.5 41.0 30.0 41.0 42-43 36.245531729965364 39.0 35.0 41.0 30.0 41.0 44-45 35.99204433712221 39.0 35.0 40.5 29.5 41.0 46-47 35.604445006426644 39.0 35.0 40.0 27.5 41.0 48-49 35.248670168688776 39.0 35.0 40.0 26.5 41.0 50-51 34.27506914631766 37.5 34.0 39.5 24.5 40.5 52-53 34.36976860382751 38.0 34.0 39.5 24.5 40.5 54-55 34.66127204671873 38.0 34.0 40.0 24.0 41.0 56-57 34.395898116445714 38.0 34.0 40.0 23.5 41.0 58-59 34.11989228765359 38.0 34.0 40.0 23.0 41.0 60-61 33.78339985047454 38.0 33.0 40.0 22.0 41.0 62-63 33.41380557025303 37.0 33.0 40.0 20.5 41.0 64-65 32.98933556925864 36.5 32.0 39.5 18.5 41.0 66-67 32.53222531448838 36.0 32.0 39.0 16.5 40.5 68-69 32.04364535212754 35.5 31.0 39.0 12.5 40.0 70-71 31.56270432383924 35.0 31.0 38.5 8.0 40.0 72-73 31.05159426303468 35.0 30.5 37.5 7.0 40.0 74-75 30.48682015328201 35.0 29.5 37.0 2.0 39.0 76-77 29.950866523361206 34.0 29.0 36.5 2.0 39.0 78-79 29.39516092401332 34.0 29.0 36.0 2.0 38.5 80-81 28.795353907610945 34.0 28.0 35.5 2.0 37.0 82-83 28.20536671180257 34.0 26.5 35.0 2.0 37.0 84-85 27.614736851150965 33.0 26.0 35.0 2.0 36.0 86-87 27.005783369773514 33.0 25.5 35.0 2.0 36.0 88-89 26.41837604057075 32.0 24.5 35.0 2.0 35.5 90-91 25.81234309340922 32.0 22.5 34.5 2.0 35.0 92-93 25.115292968006706 32.0 19.5 34.0 2.0 35.0 94-95 24.306432050008777 31.0 12.0 34.0 2.0 35.0 96-97 23.405637527207375 31.0 2.0 34.0 2.0 35.0 98-99 22.20544589400388 30.5 2.0 34.0 2.0 35.0 100-101 19.110890683139836 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11229514845053146 1101 2 0.05211658007394604 1101 3 0.21902167703465736 1101 4 -0.023700825554200833 1101 5 0.05587263060701275 1101 6 0.08905641948796017 1101 7 0.08042263565906893 1101 8 0.16161382208124309 1101 9 0.21327995439717995 1101 10-11 0.38747351335131697 1101 12-13 0.4632453697963115 1101 14-15 0.3480675962738147 1101 16-17 0.4415644008991464 1101 18-19 0.35342557707469524 1101 20-21 0.41850053429948986 1101 22-23 0.2876302038843477 1101 24-25 0.08509854016114815 1101 26-27 0.1995391671054776 1101 28-29 0.3163272925769647 1101 30-31 0.2724127356123702 1101 32-33 0.32144053321984956 1101 34-35 0.15480175856126976 1101 36-37 0.13595223307736148 1101 38-39 0.22346960259880433 1101 40-41 0.3230229630222752 1101 42-43 0.38681305039248315 1101 44-45 0.5340515130459167 1101 46-47 0.6548803785790014 1101 48-49 0.8679974920749132 1101 50-51 0.8796929749783118 1101 52-53 0.6958400499870336 1101 54-55 0.7568390431538816 1101 56-57 0.8310744030340089 1101 58-59 0.8425020180791165 1101 60-61 0.8948296247844212 1101 62-63 0.9272981899757937 1101 64-65 0.7308314104201017 1101 66-67 0.9681840709506133 1101 68-69 0.9393747455563783 1101 70-71 1.0246555840910112 1101 72-73 1.1165036923426506 1101 74-75 1.2693946128914355 1101 76-77 1.2107657040667696 1101 78-79 1.4645015455948425 1101 80-81 1.1811120519269345 1101 82-83 0.7648582290671726 1101 84-85 0.9614079772381032 1101 86-87 1.2512996390346132 1101 88-89 1.5096472077677667 1101 90-91 1.122719210267359 1101 92-93 1.4405729584930036 1101 94-95 1.5406435790804274 1101 96-97 1.5917068506905672 1101 98-99 1.4964880267234832 1101 100-101 1.5627911964107888 1102 1 0.3664665366147233 1102 2 0.32711526244673195 1102 3 0.22346208075563467 1102 4 0.24112028459065016 1102 5 0.30864952996664385 1102 6 0.22538471948532646 1102 7 0.21078318238652116 1102 8 0.1448799564265144 1102 9 0.28356658223776066 1102 10-11 0.39946282300250147 1102 12-13 0.46118108715648987 1102 14-15 0.5914229120212156 1102 16-17 0.4382203728248939 1102 18-19 0.5110077530921231 1102 20-21 0.3760158407356613 1102 22-23 0.2939290109925139 1102 24-25 0.4572766218716069 1102 26-27 0.42354018607052524 1102 28-29 0.38865734062643753 1102 30-31 0.5886685221918952 1102 32-33 0.5184538017259257 1102 34-35 0.5357211988332296 1102 36-37 0.6793696969084948 1102 38-39 0.6706821622214321 1102 40-41 0.8106098166163278 1102 42-43 0.9720904328820978 1102 44-45 1.28927535594962 1102 46-47 1.008495288848593 1102 48-49 0.9991782617448877 1102 50-51 0.8496873091812773 1102 52-53 1.218470846448767 1102 54-55 1.2747285776801007 1102 56-57 1.3894842685481876 1102 58-59 1.3631805302144642 1102 60-61 1.2945834480993952 1102 62-63 1.2165711671984099 1102 64-65 0.9430468029412467 1102 66-67 1.0702787688186959 1102 68-69 1.1251376736754253 1102 70-71 1.197651253489557 1102 72-73 0.987748454774465 1102 74-75 1.1358563885258732 1102 76-77 1.094463141721043 1102 78-79 1.1911504462545324 1102 80-81 1.4999525838969596 1102 82-83 1.5007574218008983 1102 84-85 1.4838334434585327 1102 86-87 1.7231869643392024 1102 88-89 1.8348387029232924 1102 90-91 1.7125166031223067 1102 92-93 1.764310671619203 1102 94-95 1.7798324477657026 1102 96-97 1.435402224062198 1102 98-99 1.8790275994608976 1102 100-101 1.6956008827276676 1103 1 0.10203666654901866 1103 2 0.13601180906003663 1103 3 0.00192658349759256 1103 4 0.11869056273896206 1103 5 0.10101280253223166 1103 6 0.12352463352119258 1103 7 0.1420032308030983 1103 8 -0.09036533777831579 1103 9 -0.01977317370315035 1103 10-11 0.014655995276591227 1103 12-13 0.10103089694848677 1103 14-15 -0.03462878996979413 1103 16-17 0.06721535825051461 1103 18-19 0.27819255127403153 1103 20-21 -0.030263493289332644 1103 22-23 0.1078531007113952 1103 24-25 0.08402801755364209 1103 26-27 0.17045980466644295 1103 28-29 -0.14486002511636542 1103 30-31 0.09944491663880939 1103 32-33 -0.10731505850824874 1103 34-35 0.2375664633952539 1103 36-37 0.2555424403500197 1103 38-39 0.274924788573891 1103 40-41 0.3389792153614408 1103 42-43 0.06342670579351761 1103 44-45 0.2090106704103647 1103 46-47 0.12820404829723486 1103 48-49 0.033699777480279636 1103 50-51 -0.060135575063569036 1103 52-53 -0.053739642163456836 1103 54-55 0.21492113239578003 1103 56-57 -0.09251452103730884 1103 58-59 0.1290931580490593 1103 60-61 -0.036140083346680285 1103 62-63 0.01923778446040103 1103 64-65 0.03775000436410636 1103 66-67 0.45585939724614377 1103 68-69 0.41909362940883454 1103 70-71 0.468907614341294 1103 72-73 0.6213745107993596 1103 74-75 0.5873570554889689 1103 76-77 0.6452511462662756 1103 78-79 0.518039557464455 1103 80-81 0.5548646435778082 1103 82-83 0.8647512956213603 1103 84-85 0.8962914494859042 1103 86-87 0.969660705755139 1103 88-89 0.9742920402090007 1103 90-91 0.8625079573044125 1103 92-93 1.1231849861293561 1103 94-95 1.1859685082370213 1103 96-97 1.1479942402669288 1103 98-99 1.4515938017236323 1103 100-101 1.5615715610538352 1104 1 0.07560810914814553 1104 2 0.21724433873095705 1104 3 0.10427341739935869 1104 4 0.12406111373758932 1104 5 0.18445534928888918 1104 6 0.23384219255208905 1104 7 0.2112901467949584 1104 8 0.09615329614832291 1104 9 0.22536852007593922 1104 10-11 0.2228008508988566 1104 12-13 0.2594745893004955 1104 14-15 0.45137792649208563 1104 16-17 0.4564644368645432 1104 18-19 0.5323831390626381 1104 20-21 0.3505684053221998 1104 22-23 0.41767248942171875 1104 24-25 0.4424730191574682 1104 26-27 0.527348105846734 1104 28-29 0.3818925721119584 1104 30-31 0.395178719557137 1104 32-33 0.4687852039967382 1104 34-35 0.45141507460638053 1104 36-37 0.4005289877874887 1104 38-39 0.45894406076079974 1104 40-41 0.4473171856283287 1104 42-43 0.35922793518098217 1104 44-45 0.17093304486947858 1104 46-47 0.4218914906083171 1104 48-49 0.5392248201946188 1104 50-51 0.3110333904310991 1104 52-53 0.439340961925339 1104 54-55 0.6818494312194119 1104 56-57 0.6420366347047377 1104 58-59 0.7778562423845159 1104 60-61 0.8573732284712818 1104 62-63 0.8497479344352001 1104 64-65 0.8429346416031578 1104 66-67 0.7170885228623121 1104 68-69 0.7269897271837635 1104 70-71 0.3947745027079357 1104 72-73 0.7566634299465171 1104 74-75 0.7474405857555979 1104 76-77 1.0542254129215927 1104 78-79 1.2726464873343808 1104 80-81 1.3274842600979468 1104 82-83 1.0031627803783962 1104 84-85 0.9873368216080038 1104 86-87 1.2006978295907231 1104 88-89 1.3019440934431792 1104 90-91 1.192950680810064 1104 92-93 1.4659215843003324 1104 94-95 1.6438014346112446 1104 96-97 1.6299180549030545 1104 98-99 1.7718433451220577 1104 100-101 1.5016785269281883 1105 1 -0.0432556856074946 1105 2 -0.1083815324250672 1105 3 0.05318964485819677 1105 4 0.14710319057355292 1105 5 -0.023081239264122644 1105 6 -0.07108963948903124 1105 7 0.04665390888224863 1105 8 0.1721235461701056 1105 9 0.05212961642579472 1105 10-11 -0.0816402752881018 1105 12-13 -0.05370940429650517 1105 14-15 0.10958530518360732 1105 16-17 0.0056616121411394715 1105 18-19 0.05013702659639563 1105 20-21 -0.15957443704211727 1105 22-23 -0.18336799755449817 1105 24-25 0.013700232982721161 1105 26-27 0.0474718100021434 1105 28-29 -0.29617171920262564 1105 30-31 -0.2520098966115185 1105 32-33 -0.1237737623766364 1105 34-35 0.07598562191014935 1105 36-37 0.1919898892161811 1105 38-39 0.08394338017016167 1105 40-41 -0.13603815346915127 1105 42-43 0.02123573202739948 1105 44-45 -0.18835552011875478 1105 46-47 0.07520201961781936 1105 48-49 0.028558603625235435 1105 50-51 -0.09422294722897817 1105 52-53 0.013609735337659856 1105 54-55 0.1578749452014705 1105 56-57 0.4701466617105865 1105 58-59 0.2862574532913911 1105 60-61 0.3689691318600765 1105 62-63 0.08509413728388182 1105 64-65 0.02658045678740706 1105 66-67 0.2850223585622871 1105 68-69 0.3645874600002088 1105 70-71 0.37166300562630994 1105 72-73 0.6247676855436914 1105 74-75 0.7428275423720265 1105 76-77 0.5933947656526684 1105 78-79 0.655159527451115 1105 80-81 0.8173938232132016 1105 82-83 0.8843044303479068 1105 84-85 0.9925807725183624 1105 86-87 0.7012184814332194 1105 88-89 0.8081293012138921 1105 90-91 0.9856870091052095 1105 92-93 1.1467225305935607 1105 94-95 1.558678601611856 1105 96-97 1.402015471925445 1105 98-99 1.2930233259566215 1105 100-101 1.195333788710574 1106 1 -0.186585878469387 1106 2 -0.37828080642196227 1106 3 0.0919317474961936 1106 4 0.23965473125189618 1106 5 0.2026586188604611 1106 6 0.15176837425514833 1106 7 0.06748839680066965 1106 8 0.17676497822507997 1106 9 -0.04336265247245308 1106 10-11 -0.13091046205378376 1106 12-13 -0.01979750837542582 1106 14-15 0.1121761941217585 1106 16-17 0.22720686263991752 1106 18-19 0.2997664047830497 1106 20-21 0.18463797428574935 1106 22-23 0.28700303990947873 1106 24-25 0.17543162568943416 1106 26-27 0.1538783717401202 1106 28-29 0.3296872921030811 1106 30-31 0.4420054270417708 1106 32-33 0.4483520135045467 1106 34-35 0.42327384791325073 1106 36-37 0.47064380464514244 1106 38-39 0.4022365108507202 1106 40-41 0.5539748009755456 1106 42-43 0.49414916585581636 1106 44-45 0.4974316529027476 1106 46-47 0.5934819880283726 1106 48-49 0.6362259722796324 1106 50-51 0.45564127356463757 1106 52-53 0.5507896843511801 1106 54-55 0.6809470190210263 1106 56-57 0.6005224098817195 1106 58-59 0.4790223612940494 1106 60-61 0.5730293457815989 1106 62-63 0.6539864976252687 1106 64-65 0.8283965036727992 1106 66-67 0.8433658522443892 1106 68-69 0.7576578712172335 1106 70-71 0.8120136762093288 1106 72-73 0.7209838792923087 1106 74-75 0.5933214738728054 1106 76-77 0.8420369735888258 1106 78-79 0.9618144404415858 1106 80-81 1.1159314257403565 1106 82-83 1.4482314975170496 1106 84-85 1.042207726076331 1106 86-87 1.027218467578198 1106 88-89 1.487247013472814 1106 90-91 1.3939650378128157 1106 92-93 1.642210989361022 1106 94-95 1.268415910412564 1106 96-97 1.0556053078819936 1106 98-99 1.0050328582111128 1106 100-101 0.7321950722397226 1107 1 0.06387224081391096 1107 2 0.09902858463178887 1107 3 0.06985979535986786 1107 4 0.11146355846653222 1107 5 -0.02381284461529276 1107 6 -0.012038636144552584 1107 7 0.1076384406548172 1107 8 -0.18500438025129995 1107 9 0.13109073682713301 1107 10-11 -0.028154698365021602 1107 12-13 0.04680794520182019 1107 14-15 0.039037646320728925 1107 16-17 0.10573477267625009 1107 18-19 0.16024363194756575 1107 20-21 0.20834466830905285 1107 22-23 0.334295045923767 1107 24-25 0.3747213450228557 1107 26-27 0.2813764922763937 1107 28-29 0.06952644133249208 1107 30-31 0.26617572211757334 1107 32-33 0.318533929931057 1107 34-35 0.11253976070614158 1107 36-37 -0.01936567098447739 1107 38-39 0.15263589689256918 1107 40-41 0.053996336497412756 1107 42-43 0.23648447784679405 1107 44-45 0.4545426822223817 1107 46-47 0.533996961089386 1107 48-49 0.7250991554646973 1107 50-51 0.46932220327269647 1107 52-53 0.4706540497189238 1107 54-55 0.7044364000509731 1107 56-57 0.7932026817774727 1107 58-59 0.8125429884084454 1107 60-61 0.8248637806895118 1107 62-63 0.7191608178858857 1107 64-65 0.690590699262323 1107 66-67 0.882012325117465 1107 68-69 0.9775159717058983 1107 70-71 0.8663849955928704 1107 72-73 0.9991405952426895 1107 74-75 0.8875808868536339 1107 76-77 1.0943354011041713 1107 78-79 1.1240924287889058 1107 80-81 1.1969402278954746 1107 82-83 1.3080607012509162 1107 84-85 1.1888861654949494 1107 86-87 1.1473671018345577 1107 88-89 0.988417762752352 1107 90-91 1.0024355458945102 1107 92-93 1.1302352814296874 1107 94-95 1.148613802280753 1107 96-97 1.0533010070425028 1107 98-99 1.170218142010139 1107 100-101 1.4898049425567272 1108 1 -0.039721554176011864 1108 2 0.15329542885868364 1108 3 -0.02356627719987614 1108 4 0.04292591089958364 1108 5 0.01184616776593117 1108 6 0.19562584701122177 1108 7 0.006879010767114835 1108 8 -0.16501985678456066 1108 9 0.12646577281907412 1108 10-11 0.1247840433568328 1108 12-13 0.02092524292713449 1108 14-15 0.2653099613221741 1108 16-17 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0.238526958171132 2316 86-87 0.5433490965242349 2316 88-89 0.5285736151696554 2316 90-91 0.541600898809655 2316 92-93 0.54721828563369 2316 94-95 0.467100014804263 2316 96-97 0.5377624380636306 2316 98-99 0.7672110339229583 2316 100-101 1.4108052606228831 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3458.0 3 629.0 4 606.0 5 617.0 6 706.0 7 854.0 8 1107.0 9 1281.0 10 1753.0 11 2288.0 12 3095.0 13 4167.0 14 4578.0 15 5857.0 16 7173.0 17 9018.0 18 8359.0 19 6282.0 20 6109.0 21 6502.0 22 7133.0 23 7876.0 24 8968.0 25 10220.0 26 11848.0 27 13879.0 28 16427.0 29 19505.0 30 23813.0 31 29111.0 32 36497.0 33 47206.0 34 61659.0 35 83770.0 36 113608.0 37 142167.0 38 97115.0 39 9333.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.471903225846155 19.487133413986665 14.017434411733214 22.023528948433967 2 14.807391576634272 20.756727360866574 41.74218068489831 22.69370037760085 3 19.78153936253196 25.476083260127613 29.635029491447852 25.10734788589258 4 12.227288610223878 16.8293625209778 37.91383476630363 33.029514102494694 5 12.934211157687258 38.21464208069471 34.368152250275905 14.482994511342117 6 30.460170242078334 36.81866266482976 18.12709112379453 14.594075969297382 7 27.807273496661434 33.53240893367646 20.921635970890268 17.73868159877184 8 25.6627828020902 35.93431709261245 19.93084326441442 18.47205684088292 9 26.051558376852245 17.45382074514218 21.092555344368378 35.40206553363719 10-11 24.68182693378297 27.465153059561064 27.141582016339722 20.711437990316245 12-13 25.65352692628742 24.958309727812196 28.047726519195983 21.340436826704412 14-15 22.401056934471786 26.299056361227528 26.890199122419634 24.409687581881055 16-17 21.188893037021366 29.84988612453787 27.491314036111714 21.46990680232905 18-19 21.083920943521775 28.18757771767406 30.13453229180888 20.593969046995287 20-21 21.910561180528948 27.084969523051594 30.25542087463399 20.749048421785467 22-23 21.580646371440643 27.07199309238262 30.2023147160171 21.14504582015964 24-25 21.411201790908923 27.294386804346892 30.33684093998118 20.957570464763005 26-27 21.29515232654748 27.376837164738 30.453809439762768 20.874201068951752 28-29 20.840600959941742 27.809281076945407 30.510624060538817 20.83949390257403 30-31 21.19964423274461 27.468504387427096 30.324422339526457 21.007429040301837 32-33 20.860482766849664 27.60157896225916 30.314697658741924 21.22324061214925 34-35 21.47327257842998 27.519978448554227 30.201669621994775 20.805079351021018 36-37 21.381562560540228 27.414644046531794 30.191545432460938 21.01224796046704 38-39 21.10074140399384 27.81501060202791 30.276661271357796 20.807586722620446 40-41 21.29722556348439 27.78538273645682 30.063549576541142 20.853842123517648 42-43 21.02751979867072 27.914634318843927 29.79465471253566 21.263191169949685 44-45 21.20116134690165 27.926321751638795 29.58599495831291 21.286521943146646 46-47 21.478933442890337 28.203650560721112 29.18289828296458 21.13451771342397 48-49 21.418427589905644 28.202428318118162 29.124195291343437 21.254948800632757 50-51 21.288984051161272 28.418691308255028 29.142044863912965 21.15027977667074 52-53 21.528735059485253 28.703442277373952 28.616156639275335 21.151666023865456 54-55 21.27316194401955 28.399540190012974 28.803688320666414 21.523609545301063 56-57 21.383288492582057 28.74619109847029 28.599584902759627 21.270935506188025 58-59 21.725562393313755 28.309302794626213 28.711267051352056 21.25386776070798 60-61 21.414524779641546 28.385440046635928 29.14038373793363 21.059651435788894 62-63 21.304360125105617 28.48188293056097 29.117496214977006 21.096260729356406 64-65 21.26126458515888 28.521822011099324 28.97655613460622 21.240357269135572 66-67 21.113019500625093 28.51896469766375 28.75055422214269 21.617461579568467 68-69 20.983774908758843 28.51803454096551 28.961280543111638 21.536910007164007 70-71 21.252438313437352 28.570760901382673 28.720011512037214 21.45678927314276 72-73 21.17040052640965 28.409947666517027 28.801680083143204 21.617971723930115 74-75 20.89923792562272 28.804345152291884 28.69223559856539 21.604181323520006 76-77 21.115410682151683 28.507468010494453 28.67591798780075 21.701203319553112 78-79 21.101671825711417 28.493179666987917 28.540905699878287 21.864242807422382 80-81 21.109339945684262 28.64829328531507 28.590853406541505 21.651513362459166 82-83 21.143467533841207 28.560245098159797 28.619158194578027 21.67712917342097 84-85 21.088567136628292 28.44709749659196 28.464498927936553 21.999836438843193 86-87 21.14300718092008 28.372320682992942 28.612996493782646 21.87167564230434 88-89 21.208487379868302 28.135538812347338 28.42647460353772 22.229499204246643 90-91 21.167310915998332 27.968702150189834 28.734176125427535 22.129810808384303 92-93 21.439869428157813 27.784829510023506 28.688476198558742 22.08682486325994 94-95 21.041954154459965 27.84124032380657 28.742629858389968 22.374175663343493 96-97 20.827337156126923 27.87884155086357 28.929679293693372 22.36414199931613 98-99 20.96415210493915 28.130410102281107 28.445291508226582 22.46014628455316 100-101 21.184631836442538 27.656372890548663 28.882795584533937 22.27619968847486 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1172.0 1 686.0 2 265.0 3 324.5 4 268.0 5 255.5 6 425.0 7 779.5 8 1255.0 9 1527.5 10 1439.0 11 1299.5 12 1291.5 13 1353.5 14 1465.0 15 1545.5 16 1603.5 17 1690.5 18 1794.0 19 1974.0 20 2135.5 21 2402.0 22 2819.0 23 3373.0 24 3990.0 25 4754.5 26 5968.5 27 7411.5 28 8619.0 29 10009.5 30 11686.5 31 13009.5 32 14413.5 33 16133.5 34 18228.5 35 20142.5 36 22102.0 37 24315.0 38 25802.5 39 26547.5 40 26765.0 41 27315.0 42 28151.5 43 29155.0 44 30243.5 45 30832.0 46 30575.5 47 31125.5 48 34095.0 49 33813.5 50 29868.5 51 26816.0 52 24764.5 53 23488.5 54 22021.0 55 19755.5 56 17334.0 57 15192.5 58 13401.5 59 11859.0 60 10045.0 61 7995.5 62 6109.0 63 4597.5 64 3466.5 65 2636.5 66 1913.0 67 1445.5 68 1133.0 69 802.0 70 555.5 71 406.5 72 317.5 73 246.5 74 193.0 75 153.5 76 103.5 77 63.5 78 37.0 79 26.0 80 17.5 81 11.0 82 7.5 83 6.0 84 4.0 85 3.0 86 2.5 87 2.0 88 1.5 89 3.0 90 3.0 91 1.0 92 0.5 93 1.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.251173308355134 2 0.22269226850254795 3 0.2208508219603549 4 0.22256950539973505 5 0.22220121609129645 6 0.19850793724841237 7 0.20292740894967573 8 0.2014542517159213 9 0.2101704320156351 10-11 0.2175975997358138 12-13 0.21330089113736334 14-15 0.2037867506693658 16-17 0.1997969498279475 18-19 0.18880965212619555 20-21 0.19464089950980693 22-23 0.19169458504229803 24-25 0.1942112286499619 26-27 0.18874827057478913 28-29 0.19777135863153517 30-31 0.20697859134250046 32-33 0.20108596240748264 34-35 0.2011473439588891 36-37 0.19451813640699406 38-39 0.18721373178962822 40-41 0.18549504835024805 42-43 0.19402708399574256 44-45 0.1909580064254208 46-47 0.1971575431174708 48-49 0.1997969498279475 50-51 0.2082676039220356 52-53 0.21219602321204747 54-55 0.20041076534201185 56-57 0.1859247192100931 58-59 0.17739268356459856 60-61 0.18046176113492032 62-63 0.18463570663055795 64-65 0.17978656406944954 66-67 0.1842060357707129 68-69 0.18224182612570697 70-71 0.18598610076149952 72-73 0.18684544248118962 74-75 0.1881958366121312 76-77 0.19347465003308467 78-79 0.1968506353604386 80-81 0.1906510986683886 82-83 0.18598610076149952 84-85 0.17499880305974758 86-87 0.17800649907866292 88-89 0.18549504835024805 90-91 0.18242597077992628 92-93 0.18880965212619555 94-95 0.18402189111649359 96-97 0.19138767728526584 98-99 0.19304497917323962 100-101 0.18175077371445547 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 814577.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.91722508321794 #Duplication Level Percentage of deduplicated Percentage of total 1 85.78042235947954 59.11748675481754 2 8.740329011493653 12.047184435729758 3 2.2975908257099156 4.750307522537605 4 0.960343629220773 2.6473687220896966 5 0.5232523469749985 1.803054988589901 6 0.3369784733594522 1.3934172778027518 7 0.23567394967683022 1.1369396245290362 8 0.17851656710247646 0.98422931488678 9 0.14247066285447604 0.8836814465727465 >10 0.7462652056024048 9.293740714051095 >50 0.039961680977202416 1.8579705057836233 >100 0.016054665506993627 1.9349229150981258 >500 0.001248696190795454 0.5567866965771984 >1k 8.919258505681813E-4 1.5929090809341486 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4298 0.5276358158897194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3488 0.4281977026112939 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2201 0.27020158929112903 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1829 0.224533715044741 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1141 0.14007270030948576 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 914 0.11220547597096406 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.138155140643549E-5 0.0 20-21 0.0 0.0 0.0 1.8414465421930646E-4 0.0 22-23 0.0 0.0 0.0 3.682893084386129E-4 0.0 24-25 0.0 0.0 0.0 4.296708598450484E-4 0.0 26-27 0.0 0.0 0.0 6.138155140643549E-4 0.0 28-29 0.0 0.0 0.0 0.001289012579535145 0.0 30-31 0.0 0.0 0.0 0.003375985327353952 0.0 32-33 0.0 0.0 0.0 0.006751970654707904 0.0 34-35 0.0 0.0 0.0 0.010803153047532646 0.0 36-37 0.0 0.0 0.0 0.015345387851608872 0.0 38-39 0.0 0.0 0.0 0.021544924543658854 6.138155140643549E-5 40-41 0.0 0.0 0.0 0.027437553478676664 1.2276310281287097E-4 42-43 0.0 0.0 0.0 0.041739454956376126 1.2276310281287097E-4 44-45 0.0 0.0 0.0 0.059110434004397375 1.2276310281287097E-4 46-47 0.0 0.0 0.0 0.07660417615523149 1.2276310281287097E-4 48-49 0.0 0.0 0.0 0.09839462690451609 1.2276310281287097E-4 50-51 0.0 0.0 0.0 0.12834882399085662 1.2276310281287097E-4 52-53 0.0 0.0 0.0 0.18451294352774508 1.2276310281287097E-4 54-55 0.0 0.0 0.0 0.22232397919410934 1.2276310281287097E-4 56-57 0.0 0.0 0.0 0.30629394151811307 1.2276310281287097E-4 58-59 0.0 0.0 0.0 0.4303460569105192 1.2276310281287097E-4 60-61 0.0 0.0 0.0 0.5494262666390041 1.2276310281287097E-4 62-63 0.0 0.0 0.0 0.7441899292516239 1.2276310281287097E-4 64-65 0.0 0.0 0.0 0.8756692123642087 1.2276310281287097E-4 66-67 0.0 0.0 0.0 1.1052975961756837 1.8414465421930646E-4 68-69 0.0 0.0 0.0 1.3964916760478139 2.4552620562574195E-4 70-71 0.0 0.0 0.0 1.6142120388864405 2.4552620562574195E-4 72-73 0.0 0.0 0.0 1.9410688001257093 2.4552620562574195E-4 74-75 0.0 0.0 0.0 2.2793425299265753 2.4552620562574195E-4 76-77 0.0 0.0 0.0 2.5291040625993615 2.4552620562574195E-4 78-79 0.0 0.0 0.0 2.7840830271416945 2.4552620562574195E-4 80-81 0.0 0.0 0.0 3.2154725704261233 2.4552620562574195E-4 82-83 0.0 0.0 0.0 3.5760891849389314 2.4552620562574195E-4 84-85 0.0 0.0 0.0 4.044798711478473 2.4552620562574195E-4 86-87 0.0 0.0 0.0 4.678317703544294 2.4552620562574195E-4 88-89 0.0 0.0 0.0 5.495428915866762 2.4552620562574195E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGTGA 55 0.0025742007 34.55908 1 GTACATG 3900 0.0 28.145714 1 TACATGG 4035 0.0 26.258673 2 TACCTGG 695 0.0 25.29463 2 CATGGGG 1505 0.0 25.256021 4 ACATGGG 3995 0.0 25.09287 3 GAGTACT 3610 0.0 23.227127 12-13 GTACCTG 800 0.0 23.165382 1 GTATCAA 6845 0.0 23.0478 1 GTGTAAG 360 0.0 22.439402 1 GTGTAGG 345 0.0 22.037674 1 GTACTTT 3805 0.0 21.720633 14-15 GGTATCA 5310 0.0 20.761482 1 ATCAACG 7880 0.0 19.896305 3 CAACGCA 7880 0.0 19.776937 5 TCAACGC 7930 0.0 19.772072 4 TATCAAC 7925 0.0 19.724592 2 AACGCAG 8075 0.0 19.35581 6 AGTACTT 3760 0.0 19.268148 12-13 CATGGGA 2770 0.0 19.039461 4 >>END_MODULE