FastQCFastQC Report
Thu 26 May 2016
SRR937259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814577
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55390.6799848264804923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48870.5999432834465005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32330.3968931113940119No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15010.18426741732211932No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12620.15492703574984315No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9380.11515179043847298No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9170.11257376527940269No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72900.026.6010531
GGTATCA52550.024.6015431
GTACATG38550.023.0559731
ATCAACG84100.022.8169823
TATCAAC84400.022.7921542
TACATGG37400.022.732872
TCAACGC84250.022.7199824
GAGTACT46400.022.67402312-13
CAACGCA84700.022.5992745
TACCTGG7150.022.5863322
AACGCAG86300.022.1802836
ACATGGG36350.022.0828513
GTACTTT48750.021.43487214-15
AGTACTT47900.021.17069812-13
ACGCAGA95550.019.8342157
CGCAGAG96300.019.58118
ATGGGAG10750.019.440935
TATATGG4200.019.2252712
CATGGGG12400.019.152314
AGAGTAC73700.019.04420510-11
Produced by FastQC (version 0.11.5)