##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937259_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 814577 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25320012718257 33.0 31.0 34.0 31.0 34.0 2 32.44401327314667 34.0 31.0 34.0 31.0 34.0 3 32.50524259830562 34.0 31.0 34.0 31.0 34.0 4 36.12736303627527 37.0 35.0 37.0 35.0 37.0 5 36.06797638528954 37.0 35.0 37.0 35.0 37.0 6 36.110950837060216 37.0 35.0 37.0 35.0 37.0 7 36.056784073206096 37.0 35.0 37.0 35.0 37.0 8 36.039311200782734 37.0 35.0 37.0 35.0 37.0 9 37.74146336073815 39.0 37.0 39.0 35.0 39.0 10-11 37.66723403680683 39.0 37.0 39.0 35.0 39.0 12-13 37.56361154316903 39.0 37.0 39.0 35.0 39.0 14-15 38.79445221262078 40.0 38.0 41.0 35.0 41.0 16-17 38.74521745642217 40.0 38.0 41.0 35.0 41.0 18-19 38.74989350300831 40.0 38.0 41.0 35.0 41.0 20-21 38.664822969467586 40.0 38.0 41.0 34.0 41.0 22-23 38.612073505635436 40.0 38.0 41.0 34.0 41.0 24-25 38.64127516490154 40.0 38.0 41.0 34.0 41.0 26-27 38.48311823191669 40.0 38.0 41.0 34.0 41.0 28-29 38.335395548855416 40.0 38.0 41.0 34.0 41.0 30-31 38.34953478922189 40.0 38.0 41.0 34.0 41.0 32-33 38.22003628877319 40.0 38.0 41.0 34.0 41.0 34-35 38.07730269821023 40.0 37.0 41.0 34.0 41.0 36-37 37.90687866217681 40.0 37.0 41.0 33.0 41.0 38-39 37.781803930137976 40.0 37.0 41.0 33.0 41.0 40-41 37.576226065798565 40.0 37.0 41.0 33.0 41.0 42-43 37.34553455351673 40.0 37.0 41.0 33.0 41.0 44-45 37.009692146967076 40.0 36.0 41.0 31.5 41.0 46-47 36.62664303067727 40.0 36.0 41.0 31.0 41.0 48-49 36.26424696498919 39.0 36.0 41.0 30.0 41.0 50-51 35.98156589248162 39.0 35.5 41.0 29.5 41.0 52-53 35.73418228111032 39.0 35.0 40.0 28.5 41.0 54-55 35.51862009361915 39.0 35.0 40.0 28.0 41.0 56-57 35.22811410093828 38.5 35.0 40.0 27.0 41.0 58-59 34.97269012014825 38.0 34.5 40.0 26.5 41.0 60-61 34.62669397736494 38.0 34.0 40.0 26.0 41.0 62-63 34.266273170001114 37.5 34.0 40.0 25.5 41.0 64-65 33.86571251091057 37.0 33.5 40.0 24.5 41.0 66-67 33.43659654029024 36.5 33.0 39.0 23.0 40.5 68-69 33.002375466039425 36.0 32.5 39.0 22.0 40.0 70-71 32.555995934086035 35.5 32.0 38.5 21.5 40.0 72-73 32.08716671352125 35.0 31.5 38.0 20.0 40.0 74-75 31.610449349785227 35.0 31.0 37.0 18.5 39.0 76-77 30.27536930210404 33.5 29.5 35.5 17.5 38.5 78-79 30.923999818310605 34.0 31.0 36.0 18.0 39.0 80-81 30.729689765362885 34.0 31.0 36.0 14.5 37.5 82-83 30.324958843669783 34.0 30.5 35.5 9.5 37.0 84-85 29.88981152180825 34.0 30.0 35.0 7.0 36.5 86-87 29.51845497724586 34.0 30.0 35.0 2.0 36.0 88-89 29.073618577494823 34.0 29.0 35.0 2.0 36.0 90-91 28.530877989435005 34.0 29.0 35.0 2.0 35.0 92-93 28.03481622977324 33.0 28.0 35.0 2.0 35.0 94-95 27.57915212435411 33.0 27.0 35.0 2.0 35.0 96-97 26.94117621784067 33.0 26.0 35.0 2.0 35.0 98-99 26.004844232036994 32.5 24.5 34.0 2.0 35.0 100-101 23.099245375207012 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17427911548694652 1101 2 0.2781578714294355 1101 3 0.25169548721826374 1101 4 0.1973755699621691 1101 5 0.11629722499122153 1101 6 0.2338968455046455 1101 7 0.1619974229864809 1101 8 0.1652307204338257 1101 9 0.1757263491590919 1101 10-11 0.2201524196345872 1101 12-13 0.3472958874965357 1101 14-15 0.35765459264927557 1101 16-17 0.3534443680470005 1101 18-19 0.3233668607521949 1101 20-21 0.3662970977608211 1101 22-23 0.4354665707265042 1101 24-25 0.36300487966740036 1101 26-27 0.37585995233992975 1101 28-29 0.2032257938150437 1101 30-31 0.47295023873049047 1101 32-33 0.4342952111228797 1101 34-35 0.4215123588705936 1101 36-37 0.5579232583148013 1101 38-39 0.520603150960973 1101 40-41 0.4014608055258009 1101 42-43 0.48830176036437933 1101 44-45 0.6040657837941055 1101 46-47 0.6176343395463988 1101 48-49 0.5875219772330027 1101 50-51 0.7065144202282241 1101 52-53 0.7435775071068349 1101 54-55 0.7370248667661627 1101 56-57 0.875427858332273 1101 58-59 1.054276733795838 1101 60-61 1.0836602645066762 1101 62-63 1.170474979887807 1101 64-65 0.8294482031556711 1101 66-67 0.6737331526710548 1101 68-69 0.8258294410802307 1101 70-71 0.8344222981247924 1101 72-73 1.175686249767093 1101 74-75 1.015122490313761 1101 76-77 1.0729283202011715 1101 78-79 1.1383966029837858 1101 80-81 1.0784127702034603 1101 82-83 0.9049301522918327 1101 84-85 1.0622338126211872 1101 86-87 0.9060086665159623 1101 88-89 1.077057179820951 1101 90-91 1.0698808212298978 1101 92-93 1.3147872790599102 1101 94-95 1.2568250206720535 1101 96-97 1.5012411542131154 1101 98-99 1.9465857707762275 1101 100-101 2.5938392903532907 1102 1 0.32782221507321196 1102 2 0.23454001845906447 1102 3 0.2693692602877178 1102 4 0.12824516000442543 1102 5 0.14531467037556922 1102 6 0.19446575301600433 1102 7 0.12631937202066013 1102 8 0.23146674260510025 1102 9 0.35144731085127034 1102 10-11 0.3080694486487374 1102 12-13 0.3971312938966918 1102 14-15 0.40806975502487575 1102 16-17 0.4622463374604777 1102 18-19 0.3589647560623277 1102 20-21 0.40191914956992036 1102 22-23 0.41124748445705706 1102 24-25 0.17300248597796042 1102 26-27 0.32403492884223795 1102 28-29 0.22680308582757647 1102 30-31 0.3586554108565281 1102 32-33 0.2885475235586412 1102 34-35 0.3709775120227974 1102 36-37 0.40856746822159806 1102 38-39 0.4116276939639363 1102 40-41 0.367071284175843 1102 42-43 0.3529968175054705 1102 44-45 0.3540394312497668 1102 46-47 0.33204742446307733 1102 48-49 0.30646275867378847 1102 50-51 0.33276177399356044 1102 52-53 0.48992493465929954 1102 54-55 0.571569319116378 1102 56-57 0.5742127864337405 1102 58-59 0.615770945020266 1102 60-61 0.6809824067928432 1102 62-63 0.48307380192907345 1102 64-65 0.5526476655637609 1102 66-67 0.6081010429741909 1102 68-69 0.7746802505425521 1102 70-71 0.5893326555531289 1102 72-73 0.6822128965815146 1102 74-75 0.6463378476981809 1102 76-77 0.6485930631321004 1102 78-79 0.610805489061736 1102 80-81 0.6867338056827705 1102 82-83 0.7686556554058228 1102 84-85 0.9119210957617092 1102 86-87 0.7098227580998397 1102 88-89 0.720424903224643 1102 90-91 0.765088940448809 1102 92-93 1.0189761257847287 1102 94-95 0.9386449493445035 1102 96-97 1.1577027980848307 1102 98-99 1.5293843670640364 1102 100-101 2.2425066202256545 1103 1 0.1938812042535858 1103 2 0.16773007823871922 1103 3 -0.020586769552700446 1103 4 -3.515002396383693E-4 1103 5 -0.03285710967482203 1103 6 0.09782403570954301 1103 7 0.14488688350724743 1103 8 0.10791676977766684 1103 9 0.12461810280819208 1103 10-11 0.0875436594090715 1103 12-13 0.1661292436027324 1103 14-15 0.19495713707121354 1103 16-17 0.18561351299528184 1103 18-19 0.14448918667346078 1103 20-21 0.2535791466056452 1103 22-23 0.32781904712688714 1103 24-25 0.2531794782214263 1103 26-27 0.12205935945337387 1103 28-29 0.1353420661151148 1103 30-31 0.08421924169493167 1103 32-33 0.13486255246037615 1103 34-35 0.18600332596479774 1103 36-37 0.4324404241032269 1103 38-39 0.3487046664455349 1103 40-41 0.4947940992541504 1103 42-43 0.4510823864002944 1103 44-45 0.44092990108821795 1103 46-47 0.7415084194732131 1103 48-49 0.8235252802883295 1103 50-51 0.6924413846847344 1103 52-53 0.728760095965562 1103 54-55 0.5711110907679711 1103 56-57 0.6138365698436559 1103 58-59 0.7987331257425652 1103 60-61 0.9142836270882881 1103 62-63 0.7453848290522345 1103 64-65 0.7144751444432629 1103 66-67 0.7798378642774892 1103 68-69 0.8509795934603943 1103 70-71 0.7465259760412479 1103 72-73 0.6356539321034766 1103 74-75 0.5745287720596259 1103 76-77 0.35703385163069257 1103 78-79 0.42298868159174674 1103 80-81 0.5623982985437941 1103 82-83 0.7241235176655927 1103 84-85 0.6650829948773627 1103 86-87 0.7218330342719632 1103 88-89 0.9758310096423095 1103 90-91 0.9602478999863813 1103 92-93 1.080200250086886 1103 94-95 0.8831900558115713 1103 96-97 1.1408879173971194 1103 98-99 1.5464746788075239 1103 100-101 2.10775846054975 1104 1 0.10723099051822516 1104 2 0.17309931998552486 1104 3 0.19004566454283633 1104 4 0.015576857500334995 1104 5 0.0528596075071448 1104 6 0.07581551248014051 1104 7 0.014205221542972879 1104 8 0.1301452473141822 1104 9 0.16439317334413772 1104 10-11 0.16333231997899134 1104 12-13 0.08048846302679635 1104 14-15 0.13282135456412192 1104 16-17 0.006545831222034337 1104 18-19 0.1734714397649313 1104 20-21 0.1650881248000502 1104 22-23 0.016143139324398703 1104 24-25 -0.04280766492561838 1104 26-27 0.117519528739642 1104 28-29 0.24029692965834926 1104 30-31 0.10077931344193303 1104 32-33 0.12058319739782775 1104 34-35 0.10203773310283282 1104 36-37 0.26352664488236854 1104 38-39 0.1753859094492185 1104 40-41 0.35279460986236444 1104 42-43 0.30229311702827744 1104 44-45 0.30073194221181865 1104 46-47 0.38165008052100546 1104 48-49 0.32850240111263673 1104 50-51 0.4231309021599756 1104 52-53 0.4013568005787107 1104 54-55 0.5705324014599782 1104 56-57 0.4795459552153858 1104 58-59 0.29847716701017646 1104 60-61 0.4644541571388814 1104 62-63 0.4903098416537901 1104 64-65 0.3740389017341599 1104 66-67 0.3217895659775465 1104 68-69 0.22169097544116312 1104 70-71 0.3022117294174862 1104 72-73 0.3604465793452043 1104 74-75 0.3961968898137336 1104 76-77 0.5615573166965824 1104 78-79 0.23699243914449397 1104 80-81 0.5263624512287457 1104 82-83 0.43909612157109024 1104 84-85 0.45319695215647826 1104 86-87 0.5341589856070748 1104 88-89 0.5947424976724776 1104 90-91 0.838630843053565 1104 92-93 1.055332975490952 1104 94-95 1.0878987800111304 1104 96-97 1.337589257416365 1104 98-99 1.4214856437915877 1104 100-101 1.9262666990168782 1105 1 0.052982471483467464 1105 2 0.0797590355530815 1105 3 0.02230729447575186 1105 4 0.04598020273946446 1105 5 0.08561915755505822 1105 6 0.00563028293053236 1105 7 -0.04475434592805527 1105 8 0.02420178534013928 1105 9 0.05764389204785658 1105 10-11 0.04845406403335062 1105 12-13 0.026872212700105536 1105 14-15 0.05603984049495381 1105 16-17 0.17124740583654585 1105 18-19 0.11764531161787772 1105 20-21 0.21161466239043136 1105 22-23 0.1921636169356944 1105 24-25 0.05795173811470988 1105 26-27 0.11697029209010879 1105 28-29 -0.049238580839080726 1105 30-31 -0.07730879854517525 1105 32-33 -0.011933245672132387 1105 34-35 0.03897964877493365 1105 36-37 0.0622800750592063 1105 38-39 0.17311273669898242 1105 40-41 0.07687363460319574 1105 42-43 -0.27594281924666575 1105 44-45 -0.18402146618613102 1105 46-47 -0.31351881485316113 1105 48-49 -0.3863791216680923 1105 50-51 -0.387524016604722 1105 52-53 -0.3034778431184719 1105 54-55 -0.4371780099434517 1105 56-57 -0.347128025532065 1105 58-59 -0.2830004225011109 1105 60-61 -0.1817312684208261 1105 62-63 -0.12329639037861995 1105 64-65 -0.3211504113593122 1105 66-67 -0.1286110296753833 1105 68-69 -0.18852754860274246 1105 70-71 -0.2419306991477228 1105 72-73 -0.1892481076919914 1105 74-75 -0.131974118113785 1105 76-77 -0.06454796250892159 1105 78-79 -0.06707912632288071 1105 80-81 -0.11708563876167588 1105 82-83 0.02239497164513793 1105 84-85 -0.1258727076570949 1105 86-87 0.20928857006564883 1105 88-89 0.14730791604515758 1105 90-91 0.45859855583342224 1105 92-93 0.3852955702291716 1105 94-95 0.19492165874621037 1105 96-97 0.5683598493668818 1105 98-99 0.4441146021067688 1105 100-101 0.6516946544136921 1106 1 -0.060635628716042334 1106 2 0.051678371620475616 1106 3 -0.10112016063397533 1106 4 0.0448718010546969 1106 5 0.06363470288869166 1106 6 0.15202936295712988 1106 7 0.019848153517742162 1106 8 -0.033961593068191576 1106 9 -0.06508388449836389 1106 10-11 0.029541960286948665 1106 12-13 -0.2771017537153213 1106 14-15 -0.02878059843211389 1106 16-17 -0.03901293000915729 1106 18-19 0.10001133356423253 1106 20-21 0.06381968899206925 1106 22-23 0.050071716581001624 1106 24-25 0.05813358526611978 1106 26-27 0.2212414487072607 1106 28-29 0.2824402320627044 1106 30-31 0.17404033413050257 1106 32-33 0.09096106822722305 1106 34-35 -0.4572310029652584 1106 36-37 0.10201281170296994 1106 38-39 0.34709716365531307 1106 40-41 0.3044666017945019 1106 42-43 0.03374634335586535 1106 44-45 0.12580105099409877 1106 46-47 0.21798057552591388 1106 48-49 0.13548410156401047 1106 50-51 0.02473386690225965 1106 52-53 -0.09699992802204349 1106 54-55 0.006059700652869537 1106 56-57 0.08249193124026988 1106 58-59 0.14656757794225683 1106 60-61 0.17746015255013248 1106 62-63 0.31627320339216425 1106 64-65 0.5149065881973272 1106 66-67 0.25294759091871555 1106 68-69 0.4000250866222643 1106 70-71 0.30867303339496033 1106 72-73 0.5449866102197767 1106 74-75 0.40008640061820344 1106 76-77 0.25523977856844127 1106 78-79 0.2821089694430263 1106 80-81 0.196096721025512 1106 82-83 0.1711009126781704 1106 84-85 0.43836263373062323 1106 86-87 0.49712663257950496 1106 88-89 0.6523598030990279 1106 90-91 0.6734102660972248 1106 92-93 0.8177350237871401 1106 94-95 0.21683683830728384 1106 96-97 0.8054712160261595 1106 98-99 1.1055100105527913 1106 100-101 1.4473746588472984 1107 1 0.010235815965074835 1107 2 0.11816831648953752 1107 3 0.039761307853680705 1107 4 -0.004605081541470213 1107 5 0.065290980297533 1107 6 0.011692155840229645 1107 7 0.0474801858445133 1107 8 0.07802703617626605 1107 9 -0.07697149256753022 1107 10-11 0.044377977076827335 1107 12-13 0.08929382641248651 1107 14-15 -0.028042517364575303 1107 16-17 0.09594431219105815 1107 18-19 0.058228505597810454 1107 20-21 0.1770201807850782 1107 22-23 0.1562888259657882 1107 24-25 -0.05709320335685675 1107 26-27 -0.017462191187490816 1107 28-29 0.09049804123448979 1107 30-31 0.0492850559698752 1107 32-33 0.03515078442820396 1107 34-35 0.07812053506255978 1107 36-37 0.14620566034349025 1107 38-39 0.2012272851939656 1107 40-41 0.2973481329309564 1107 42-43 0.29593740650584266 1107 44-45 0.14828413582056044 1107 46-47 0.1030259175883117 1107 48-49 0.08519278468642 1107 50-51 0.27429140981667643 1107 52-53 0.09995025052700868 1107 54-55 0.1091482860431654 1107 56-57 0.18819806142445117 1107 58-59 0.2430760825156142 1107 60-61 0.2929238319136189 1107 62-63 0.28115072500599325 1107 64-65 0.12288177927614186 1107 66-67 0.30692931313398475 1107 68-69 0.5491135467149775 1107 70-71 0.39987009516666205 1107 72-73 0.2557508471575112 1107 74-75 0.20158566449491033 1107 76-77 0.2651970207686709 1107 78-79 0.1544405225065546 1107 80-81 0.10683785622160613 1107 82-83 0.13302415224714537 1107 84-85 0.39936767695828834 1107 86-87 0.653059473075686 1107 88-89 0.6705886434699089 1107 90-91 0.8330237230575044 1107 92-93 0.9555819414666296 1107 94-95 0.857129016605743 1107 96-97 0.8021077590658798 1107 98-99 0.9791532726753296 1107 100-101 1.528628182841782 1108 1 0.005016984983214456 1108 2 -0.08267569445201417 1108 3 -0.06501122309938268 1108 4 0.006372459489334403 1108 5 -0.017085567355792364 1108 6 0.06766861711290773 1108 7 0.02101711357270375 1108 8 0.13837651789236105 1108 9 0.0943861233265082 1108 10-11 0.044728898470438594 1108 12-13 0.12308575702002855 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0.6967808592943854 2316 84-85 0.7952805268310605 2316 86-87 0.8283554128920336 2316 88-89 0.7743248836513423 2316 90-91 1.1049426300162395 2316 92-93 1.099295986161838 2316 94-95 1.417656660703539 2316 96-97 1.5056182414201231 2316 98-99 1.625627924185455 2316 100-101 2.330533468271586 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 2.0 4 7.0 5 17.0 6 30.0 7 60.0 8 168.0 9 342.0 10 699.0 11 1138.0 12 1828.0 13 2634.0 14 3419.0 15 4987.0 16 6857.0 17 9435.0 18 8745.0 19 5526.0 20 4435.0 21 4306.0 22 4408.0 23 4538.0 24 5315.0 25 6169.0 26 7710.0 27 9366.0 28 11515.0 29 14464.0 30 17836.0 31 22992.0 32 29732.0 33 38921.0 34 54188.0 35 78655.0 36 120644.0 37 182756.0 38 139319.0 39 11409.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.47896575271802 19.828272307896377 14.875656475836285 21.817105463549318 2 15.137200212134902 20.81094950010803 41.23850935947045 22.813340928286618 3 19.93697342301587 25.202651191968346 30.271171417803348 24.58920396721243 4 12.420065874680969 17.22280398292611 37.84675972928281 32.51037041311012 5 12.942134028667814 37.89658863795957 34.54930933054413 14.61196800282848 6 30.615890210501895 36.84771359859166 17.73484888475859 14.801547306147853 7 27.480152275352733 33.73972012467821 20.94805033778268 17.832077262186385 8 25.761837125280973 35.70086069211382 19.86257898271127 18.674723199893933 9 25.82370972909866 17.1082660080017 21.21235929813879 35.855664964760855 10-11 24.742780608831332 27.242237382101386 27.12941809061636 20.885563918450924 12-13 25.67327258385791 24.75398917804057 28.364521406056735 21.208216832044783 14-15 22.528870812704017 25.81370453621941 27.510781669504542 24.14664298157203 16-17 21.02410207997525 29.207122224172792 28.154367236001015 21.614408459850942 18-19 20.704918012661786 27.3692358119613 31.318954500311204 20.606891675065707 20-21 21.460919545874173 26.15569326864528 31.740561961069318 20.642825224411226 22-23 21.20606673308179 26.241401174970708 31.634346071901433 20.918186020046072 24-25 21.144041631423427 26.302608593171673 31.83885624072371 20.714493534681193 26-27 21.046687139874816 26.49152518443995 31.827715682260553 20.634071993424673 28-29 20.589773494567552 26.815051255294335 31.975078264072188 20.620096986065924 30-31 20.919129607464015 26.542798391799405 31.811128502593377 20.726943498143203 32-33 20.73397694015046 26.60138526055273 31.73074090078765 20.933896898509165 34-35 21.174548262472427 26.53131625371205 31.57338103089088 20.72075445292465 36-37 21.127652757197907 26.48018542139049 31.617023313940855 20.775138507470746 38-39 20.922454230846192 26.76389095199103 31.68969907080608 20.6239557463567 40-41 20.95854658307318 26.752167075672407 31.643540144148435 20.645746197105982 42-43 20.799895396783207 26.88701686235776 31.23388211143803 21.079205629421004 44-45 20.988562161710924 26.90310431058083 30.886030418241617 21.22230310946663 46-47 21.242927310739194 27.28759834859074 30.41597049757113 21.053503843098934 48-49 21.218804361036465 27.436755518508377 30.16841870074898 21.176021419706178 50-51 21.189648124118406 27.551109348778567 30.130791809736834 21.12845071736619 52-53 21.334140296129156 27.905465045047922 29.582838700331582 21.17755595849134 54-55 21.104634675420495 27.603590575231067 29.89705086198113 21.39472388736731 56-57 21.315480304501598 27.82505521270549 29.54429108604834 21.31517339674457 58-59 21.552474474481848 27.5237331768513 29.65177018256101 21.272022166105845 60-61 21.24476875728139 27.686455669629762 30.113052541380377 20.95572303170848 62-63 21.134895780263868 27.70566809521997 30.06351762939538 21.09591849512078 64-65 21.03754463973326 27.66613837611423 30.01705179498071 21.279265189171802 66-67 20.86254583667351 27.872073481082822 29.696087662676458 21.569293019567212 68-69 20.929820017014965 27.70885993589311 29.82805799820029 21.533262048891633 70-71 21.056941209977694 27.745688866736966 29.718676073594025 21.478693849691314 72-73 20.998499212777254 27.503015385370855 29.787833495483827 21.710651906368064 74-75 20.79901593096785 28.024483873224998 29.409190291402776 21.76730990440437 76-77 20.907784040060058 27.61598964861517 29.66674728110418 21.809479030220594 78-79 20.81608000225884 27.678291923292704 29.571728639527016 21.93389943492144 80-81 21.019007411208516 27.9033780723001 29.29937869593666 21.778235820554716 82-83 20.907185841924736 27.891265864120385 29.45048577538689 21.751062518567988 84-85 20.806235886053535 27.757648001934747 29.33153525977641 22.104580852235305 86-87 20.730084448738424 27.57191769470535 29.354622092202455 22.343375764353766 88-89 20.756478515843195 27.52127791479504 29.237076421259133 22.485167148102637 90-91 20.65350441558697 27.279585905971874 29.542259616624168 22.52465006181699 92-93 20.760738259977117 27.042872014967518 29.64555271286234 22.550837012193025 94-95 20.349211922261492 27.113336124147867 29.79055387029096 22.746898083299676 96-97 20.23369184251458 27.177909516227437 29.886861524447657 22.701537116810318 98-99 20.166332034292708 27.384508863819253 29.39825712901996 23.050901972868076 100-101 19.783519248701783 27.350461979892778 29.90686735827061 22.959151413134826 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 424.0 1 309.0 2 265.0 3 357.0 4 308.5 5 260.5 6 435.5 7 890.5 8 1424.5 9 1731.0 10 1691.0 11 1557.5 12 1559.5 13 1645.5 14 1777.0 15 1863.5 16 1919.0 17 1989.5 18 2074.5 19 2249.0 20 2440.0 21 2644.5 22 3004.5 23 3503.0 24 4116.0 25 4962.5 26 6115.5 27 7546.0 28 8724.0 29 10138.5 30 11711.5 31 12687.5 32 14063.5 33 15805.5 34 17747.5 35 19896.5 36 22230.5 37 24164.0 38 25309.0 39 26055.0 40 26525.5 41 27349.5 42 28212.5 43 28992.5 44 29983.5 45 30678.5 46 30426.0 47 31367.5 48 34949.5 49 33965.0 50 29431.5 51 26921.0 52 24933.5 53 23462.5 54 21825.0 55 19599.5 56 17167.5 57 14969.0 58 13396.0 59 11932.0 60 10028.5 61 8015.5 62 6142.5 63 4524.0 64 3227.5 65 2359.5 66 1772.5 67 1367.5 68 1028.0 69 708.0 70 489.5 71 361.5 72 287.5 73 232.0 74 190.0 75 145.5 76 95.5 77 54.5 78 33.0 79 19.0 80 11.5 81 6.5 82 2.0 83 0.5 84 0.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046772742171703845 2 1.2276310281287097E-4 3 0.0 4 0.0 5 6.138155140643549E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003621511532979694 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.2276310281287097E-4 22-23 4.296708598450484E-4 24-25 0.0 26-27 0.002025591196412371 28-29 0.0033146037759475165 30-31 2.4552620562574195E-4 32-33 1.2276310281287097E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.008654798748307404 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.524339626579194E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.682893084386129E-4 84-85 1.8414465421930646E-4 86-87 0.0 88-89 0.0 90-91 0.009329995813778195 92-93 0.0015959203365673226 94-95 0.0 96-97 0.0 98-99 1.8414465421930646E-4 100-101 0.011478350113003436 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 814577.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.47627056094854 #Duplication Level Percentage of deduplicated Percentage of total 1 84.75075897909565 56.339143841401004 2 9.228253527416971 12.269197565871965 3 2.5090596979928024 5.003787940120239 4 1.0765000424468127 2.8624683232226764 5 0.6058876630967663 2.0138576110780733 6 0.3611987411013876 1.4406687143837906 7 0.24972985328648045 1.162077650595264 8 0.18704889391522828 0.99474503040279 9 0.141819086574154 0.8484843572829042 >10 0.8224150947868877 9.937777746217611 >50 0.04494859794909681 2.0491867405694726 >100 0.02014184011436151 2.4381367991634475 >500 0.0013054896302567014 0.6045131188924701 >1k 7.459940744324008E-4 1.3492445186437145 >5k 1.864985186081002E-4 0.6867100421545101 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5539 0.6799848264804923 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4887 0.5999432834465005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3233 0.3968931113940119 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1501 0.18426741732211932 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1262 0.15492703574984315 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 938 0.11515179043847298 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 917 0.11257376527940269 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.2276310281287097E-4 7 0.0 0.0 0.0 0.0 1.2276310281287097E-4 8 0.0 0.0 0.0 0.0 1.2276310281287097E-4 9 0.0 0.0 0.0 0.0 1.2276310281287097E-4 10-11 0.0 0.0 0.0 0.0 1.8414465421930646E-4 12-13 0.0 0.0 0.0 0.0 2.4552620562574195E-4 14-15 0.0 0.0 0.0 0.0 2.4552620562574195E-4 16-17 0.0 0.0 0.0 0.0 2.4552620562574195E-4 18-19 0.0 0.0 0.0 6.138155140643549E-5 2.4552620562574195E-4 20-21 0.0 0.0 0.0 1.8414465421930646E-4 2.4552620562574195E-4 22-23 0.0 0.0 0.0 3.682893084386129E-4 2.4552620562574195E-4 24-25 0.0 0.0 0.0 4.296708598450484E-4 2.4552620562574195E-4 26-27 0.0 0.0 0.0 6.138155140643549E-4 2.4552620562574195E-4 28-29 0.0 0.0 0.0 0.001289012579535145 2.4552620562574195E-4 30-31 0.0 0.0 0.0 0.0034373668787603872 2.4552620562574195E-4 32-33 6.138155140643549E-5 0.0 0.0 0.0068747337575207745 2.4552620562574195E-4 34-35 1.2276310281287097E-4 0.0 0.0 0.011048679253158389 2.4552620562574195E-4 36-37 1.2276310281287097E-4 0.0 0.0 0.015713677160047484 2.4552620562574195E-4 38-39 1.2276310281287097E-4 0.0 0.0 0.021974595403503903 3.0690775703217743E-4 40-41 1.2276310281287097E-4 0.0 0.0 0.02798998744133458 3.682893084386129E-4 42-43 1.2276310281287097E-4 0.0 0.0 0.042844322881691974 3.682893084386129E-4 44-45 1.2276310281287097E-4 0.0 0.0 0.06009253882690034 3.682893084386129E-4 46-47 1.2276310281287097E-4 0.0 0.0 0.07826147804320524 3.682893084386129E-4 48-49 1.2276310281287097E-4 0.0 0.0 0.10054298120374133 3.682893084386129E-4 50-51 1.2276310281287097E-4 0.0 0.0 0.13104961225273976 3.682893084386129E-4 52-53 1.2276310281287097E-4 0.0 0.0 0.18825721816353763 4.296708598450484E-4 54-55 1.2276310281287097E-4 0.0 0.0 0.22686621399818557 4.910524112514839E-4 56-57 1.2276310281287097E-4 0.0 0.0 0.3113886102848472 4.910524112514839E-4 58-59 1.2276310281287097E-4 0.0 0.0 0.4377732246306979 4.910524112514839E-4 60-61 1.2276310281287097E-4 0.0 0.0 0.5585107362471564 4.910524112514839E-4 62-63 1.2276310281287097E-4 0.0 0.0 0.7551158454019693 4.910524112514839E-4 64-65 2.4552620562574195E-4 0.0 0.0 0.8887434828137795 4.910524112514839E-4 66-67 2.4552620562574195E-4 0.0 0.0 1.1211340364385443 4.910524112514839E-4 68-69 2.4552620562574195E-4 0.0 0.0 1.4149675230211507 4.910524112514839E-4 70-71 2.4552620562574195E-4 0.0 0.0 1.6348362401590029 4.910524112514839E-4 72-73 2.4552620562574195E-4 0.0 0.0 1.963288921734839 4.910524112514839E-4 74-75 2.4552620562574195E-4 0.0 0.0 2.3040179135919625 4.910524112514839E-4 76-77 2.4552620562574195E-4 0.0 0.0 2.555498129704129 4.910524112514839E-4 78-79 2.4552620562574195E-4 0.0 0.0 2.8133006456111573 4.910524112514839E-4 80-81 2.4552620562574195E-4 0.0 0.0 3.2493551868024753 4.910524112514839E-4 82-83 2.4552620562574195E-4 0.0 0.0 3.6183196923065593 4.910524112514839E-4 84-85 2.4552620562574195E-4 0.0 0.0 4.093842571052215 4.910524112514839E-4 86-87 2.4552620562574195E-4 0.0 0.0 4.744487015960431 4.910524112514839E-4 88-89 2.4552620562574195E-4 0.0 0.0 5.584125257649062 4.910524112514839E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7290 0.0 26.601053 1 GGTATCA 5255 0.0 24.601543 1 GTACATG 3855 0.0 23.055973 1 ATCAACG 8410 0.0 22.816982 3 TATCAAC 8440 0.0 22.792154 2 TACATGG 3740 0.0 22.73287 2 TCAACGC 8425 0.0 22.719982 4 GAGTACT 4640 0.0 22.674023 12-13 CAACGCA 8470 0.0 22.599274 5 TACCTGG 715 0.0 22.586332 2 AACGCAG 8630 0.0 22.180283 6 ACATGGG 3635 0.0 22.082851 3 GTACTTT 4875 0.0 21.434872 14-15 AGTACTT 4790 0.0 21.170698 12-13 ACGCAGA 9555 0.0 19.834215 7 CGCAGAG 9630 0.0 19.5811 8 ATGGGAG 1075 0.0 19.44093 5 TATATGG 420 0.0 19.225271 2 CATGGGG 1240 0.0 19.15231 4 AGAGTAC 7370 0.0 19.044205 10-11 >>END_MODULE