FastQCFastQC Report
Thu 26 May 2016
SRR937258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731069
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40880.5591811443242704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.4675345282045881No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21000.2872505878378101No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16530.2261072484266191No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9260.12666383063705342No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8170.11175415726832898No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7340.10040091974902507No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG6150.031.6857552
GTGTACG856.369599E-427.967611
GTACATG37350.026.0956131
TACATGG36950.025.7259162
GGTATCA44050.025.580361
GAGTACT31300.024.81364312-13
GTACCTG8050.024.2154331
GTATCAA60200.024.1673581
ACATGGG37850.023.7312953
ACCTGGG8350.023.3358123
CACCGCA1050.002193893822.6218227
GTACTTT34250.022.1909814-15
CATGGGA23900.021.0796434
ATCAACG69350.020.8330383
TATCAAC69700.020.7980372
CAACGCA69100.020.7737055
TCAACGC69250.020.7272874
GTACACG1150.003701656420.6717111
AACGCAG70550.020.335596
CCTTAGA3102.564775E-1019.9313622
Produced by FastQC (version 0.11.5)