FastQCFastQC Report
Thu 26 May 2016
SRR937258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731069
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53620.7334465009458752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47810.6539738383107477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31130.4258147999710014No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13520.18493466416986631No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12150.16619498296330443No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9620.13158812642855872No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7740.10587235951736429No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTG500.001614688738.0035555
CCGATCG600.003950854831.66539
ACAACCG1252.1503365E-730.4028475
GGTATCA43300.030.30071
GCCGATC650.005843932329.2295068
GGCGTAA350.00839407227.14168566-67
ACGACTA700.00839137527.1416858
GTATCAA59700.026.117461
TACCTGG5650.026.0643842
TCGGGCC950.001225458825.0023384
GTACATG33950.024.3635671
TACATGG33150.024.3612562
GTATAGG1652.4115125E-623.0482271
ACATGGG32750.022.7731233
GTCTAGC2154.3797627E-822.1102161
ATCAACG71850.021.752213
GAGTACT45450.021.68498212-13
CAACGCA72750.021.4831095
TATCAAC72600.021.4620652
TCAACGC72700.021.4325434
Produced by FastQC (version 0.11.5)