FastQCFastQC Report
Thu 26 May 2016
SRR937257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850448
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43960.5169040317573796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37230.4377692698436589No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24160.28408556431433785No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19110.2247050966079055No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11550.13581077267510772No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.10547382085677197No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8620.10135834289692021No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG7250.032.1354142
TACATGG42050.026.1199172
GTACATG43550.025.989111
ACATGGG41750.025.2826333
GTACCTG9450.025.1617531
GTATCAA73750.023.6005291
GAGTACT36050.022.72601512-13
ACCTGGG10550.022.083583
GTCTAGA2901.03682396E-1021.3180751
CTACACG1353.7708966E-421.1308864
CATGGGG15750.021.1308844
CATGGGA26900.021.0326944
TCAACGC82400.020.8872644
GTACTTT38750.020.83611314-15
ATCAACG82850.020.7750423
CAACGCA82850.020.7701425
TATCAAC83750.020.5517862
AACGCAG84100.020.502246
GGTATCA55500.020.0505161
AGTACTT37900.019.73698612-13
Produced by FastQC (version 0.11.5)