##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937257_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 850448 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.70336340375896 33.0 31.0 34.0 30.0 34.0 2 31.770532707467122 33.0 31.0 34.0 30.0 34.0 3 31.821334167403535 33.0 31.0 34.0 30.0 34.0 4 35.45325992888454 37.0 35.0 37.0 33.0 37.0 5 35.4328530374579 37.0 35.0 37.0 33.0 37.0 6 35.51533544672925 37.0 35.0 37.0 33.0 37.0 7 35.459580127180025 37.0 35.0 37.0 33.0 37.0 8 35.46141092694674 37.0 35.0 37.0 33.0 37.0 9 37.078945449927566 39.0 37.0 39.0 33.0 39.0 10-11 36.98813154948921 39.0 37.0 39.0 33.0 39.0 12-13 36.87780264049066 39.0 37.0 39.0 33.0 39.0 14-15 38.03178971553817 40.0 38.0 41.0 33.0 41.0 16-17 37.976676998476094 40.0 38.0 41.0 33.0 41.0 18-19 37.95113575433184 40.0 38.0 41.0 33.0 41.0 20-21 37.89866576204541 40.0 38.0 41.0 33.0 41.0 22-23 37.76766539517995 40.0 38.0 41.0 33.0 41.0 24-25 37.62987331382989 40.0 37.0 41.0 32.0 41.0 26-27 37.45611371888698 40.0 37.0 41.0 32.0 41.0 28-29 37.26914579139465 39.5 37.0 41.0 32.0 41.0 30-31 37.26716448271969 39.0 37.0 41.0 32.0 41.0 32-33 37.11292930314376 39.0 36.0 41.0 31.0 41.0 34-35 36.918863352021525 39.0 36.0 41.0 31.0 41.0 36-37 36.92121740541455 39.0 36.0 41.0 31.0 41.0 38-39 36.89998859424679 39.5 36.0 41.0 31.0 41.0 40-41 36.7462925422836 39.5 36.0 41.0 30.5 41.0 42-43 36.5026415489248 39.0 36.0 41.0 30.0 41.0 44-45 36.28109126013584 39.0 35.5 41.0 30.0 41.0 46-47 35.97495849246515 39.0 35.0 41.0 29.0 41.0 48-49 35.66572265441273 39.0 35.0 41.0 27.5 41.0 50-51 34.71775287848287 38.0 34.0 40.0 25.5 40.5 52-53 34.84731459183865 38.0 34.5 40.0 26.0 40.5 54-55 35.21077596749008 39.0 35.0 40.0 26.5 41.0 56-57 34.99983302917992 39.0 35.0 40.0 26.0 41.0 58-59 34.768530821402365 38.0 34.0 40.0 25.5 41.0 60-61 34.48860188982748 38.0 34.0 40.0 24.5 41.0 62-63 34.18726718153256 38.0 34.0 40.0 23.5 41.0 64-65 33.84363594246797 37.0 34.0 40.0 22.5 41.0 66-67 33.46927384155175 37.0 33.0 39.5 22.0 41.0 68-69 32.985878031343475 36.0 32.5 39.0 20.5 41.0 70-71 32.56866792561097 36.0 32.0 39.0 19.0 40.0 72-73 32.16675916693319 35.0 32.0 38.5 18.0 40.0 74-75 31.71672283314206 35.0 31.0 37.5 15.0 39.5 76-77 31.28604453182323 35.0 31.0 37.0 11.5 39.0 78-79 30.815976990950652 35.0 31.0 36.5 7.5 39.0 80-81 30.364791262957876 34.5 30.5 36.0 4.5 38.5 82-83 29.918545284367767 34.0 30.0 35.5 2.0 37.0 84-85 29.459071571689275 34.0 29.5 35.0 2.0 37.0 86-87 29.02930573062668 34.0 29.0 35.0 2.0 36.0 88-89 28.653452062912724 34.0 29.0 35.0 2.0 36.0 90-91 28.224482860798076 34.0 29.0 35.0 2.0 35.5 92-93 27.793993871465393 34.0 27.0 35.0 2.0 35.0 94-95 27.283256589468138 33.5 26.5 35.0 2.0 35.0 96-97 26.690681264462967 33.0 25.0 35.0 2.0 35.0 98-99 26.017748880589995 33.0 24.5 35.0 2.0 35.0 100-101 24.19268432637857 30.5 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05954270338840573 1101 2 0.10003314447924083 1101 3 0.14994876979896077 1101 4 0.10046786358152104 1101 5 0.2020580102413021 1101 6 0.020369728550441835 1101 7 0.10659707161730836 1101 8 0.1037556763028249 1101 9 0.10334149763995981 1101 10-11 0.18345225661773412 1101 12-13 0.17510340016220738 1101 14-15 0.22049789482009174 1101 16-17 0.31694764470596226 1101 18-19 0.3884830732452187 1101 20-21 0.3747544022184428 1101 22-23 0.09690783846387063 1101 24-25 0.23983264448317243 1101 26-27 0.3034396236751462 1101 28-29 0.35378609907755276 1101 30-31 0.18963060144564992 1101 32-33 0.15609861706020922 1101 34-35 0.064869049534245 1101 36-37 0.12782188878958323 1101 38-39 0.17771547674903587 1101 40-41 0.10654686248142298 1101 42-43 0.08874393807369074 1101 44-45 -0.03924326363247843 1101 46-47 0.08440737405145171 1101 48-49 0.3291309393344548 1101 50-51 0.28400305118869085 1101 52-53 0.17259929116779205 1101 54-55 0.30791973474384093 1101 56-57 0.3891710712387635 1101 58-59 0.3966583310650478 1101 60-61 0.4309423449098233 1101 62-63 0.43007508793474614 1101 64-65 0.3792326967988302 1101 66-67 0.3788267496076827 1101 68-69 0.23347266370831932 1101 70-71 0.40137691035103984 1101 72-73 0.22311726504187135 1101 74-75 0.3446910049078866 1101 76-77 0.36985828810809096 1101 78-79 0.5400604778883498 1101 80-81 0.5435142401098361 1101 82-83 0.5049522778565141 1101 84-85 0.3801793587349209 1101 86-87 0.5476912114784618 1101 88-89 0.4440261352630053 1101 90-91 0.6305475896061594 1101 92-93 0.49249413918152385 1101 94-95 0.5252349195689625 1101 96-97 0.6701683219705572 1101 98-99 0.574473506824436 1101 100-101 0.7575499184535168 1102 1 0.1866893127523781 1102 2 -0.02665614110111747 1102 3 -0.06906693246613571 1102 4 -0.2149559312765632 1102 5 -0.03784377519640003 1102 6 -0.1330489501506591 1102 7 -0.08425531143905118 1102 8 -0.011069277108845199 1102 9 -0.017884358354407937 1102 10-11 -0.03501482014581114 1102 12-13 0.19491465016425025 1102 14-15 0.14132544706993855 1102 16-17 0.179967351203544 1102 18-19 0.13799110372043089 1102 20-21 0.22822166647658548 1102 22-23 0.35090717978718544 1102 24-25 0.3108103554512809 1102 26-27 0.3200112930781458 1102 28-29 0.3375698542193959 1102 30-31 0.2089678161889239 1102 32-33 0.16769352764158896 1102 34-35 0.11452903215431576 1102 36-37 0.31506010964518083 1102 38-39 0.46230293156691715 1102 40-41 0.4765666390856822 1102 42-43 0.5838601113578363 1102 44-45 0.5704406693846735 1102 46-47 0.4747209954525715 1102 48-49 0.3876435202220705 1102 50-51 0.3132179705415581 1102 52-53 0.42466866090877176 1102 54-55 0.44202646971289283 1102 56-57 0.3448987051710546 1102 58-59 0.4011651305974553 1102 60-61 0.2656577036522094 1102 62-63 0.41553284377112476 1102 64-65 0.511249682179141 1102 66-67 0.6678472289090962 1102 68-69 0.6836431207549367 1102 70-71 0.5787370190000871 1102 72-73 0.6074571454692261 1102 74-75 0.2076238932540413 1102 76-77 0.2953670913871278 1102 78-79 0.619696770973384 1102 80-81 0.5040384750095939 1102 82-83 0.6500710597611672 1102 84-85 0.6734111297762979 1102 86-87 0.6764942948679078 1102 88-89 0.7821155815996086 1102 90-91 0.9631890950410593 1102 92-93 0.890905896886327 1102 94-95 0.8485970727243348 1102 96-97 1.060912773353209 1102 98-99 1.101574628368219 1102 100-101 1.203278791480539 1103 1 -0.08078488432821018 1103 2 -0.19974398088402268 1103 3 -0.06748947707476205 1103 4 -0.09066159370469506 1103 5 -0.26499735155412196 1103 6 -0.17734683901493753 1103 7 -0.10585003902532719 1103 8 -0.059468191274284266 1103 9 -0.03876793066759632 1103 10-11 -0.003939217973069731 1103 12-13 0.051094481872446806 1103 14-15 -0.013857511748518903 1103 16-17 0.05529546461532675 1103 18-19 -0.09909443310315424 1103 20-21 0.024313361564054503 1103 22-23 -0.025038374236842742 1103 24-25 -0.03620023654737281 1103 26-27 -0.08599828285628774 1103 28-29 -0.2857290453283525 1103 30-31 -0.08712881124911576 1103 32-33 -0.1534356757299875 1103 34-35 -0.09536938832767561 1103 36-37 -0.07405437200512921 1103 38-39 -0.03074335092045999 1103 40-41 0.03963077668212378 1103 42-43 -0.22319216348708437 1103 44-45 -0.36971108559050236 1103 46-47 -0.20824858449948636 1103 48-49 -0.2781152950093855 1103 50-51 -0.24347132814096284 1103 52-53 -0.17760631853229825 1103 54-55 0.008758034155846417 1103 56-57 0.04371951466842461 1103 58-59 -0.03101191029711714 1103 60-61 -0.031803391311683527 1103 62-63 0.050198569405090154 1103 64-65 0.09958987446907486 1103 66-67 0.3319272513647462 1103 68-69 0.21143467423910067 1103 70-71 0.1916905374251101 1103 72-73 0.1456295033211248 1103 74-75 0.19260219539548729 1103 76-77 0.22119933586670726 1103 78-79 0.3868230131410364 1103 80-81 0.15226681952441368 1103 82-83 0.3088254887877788 1103 84-85 0.08121922008674076 1103 86-87 0.3430835385771438 1103 88-89 0.31403685769734935 1103 90-91 0.3541648664382677 1103 92-93 0.35255687120492496 1103 94-95 0.34088422161944365 1103 96-97 0.2757942130973703 1103 98-99 0.5094392165124617 1103 100-101 0.9952595965657061 1104 1 0.14987676663107408 1104 2 0.008721832960166154 1104 3 0.05211497465095505 1104 4 0.04726750519949974 1104 5 0.16964623068083995 1104 6 0.07663328462719221 1104 7 0.09268901251886774 1104 8 0.0629257244214898 1104 9 0.1345736606968586 1104 10-11 0.010643627774904019 1104 12-13 0.1394606475392024 1104 14-15 0.2688915753965446 1104 16-17 0.32235191415665554 1104 18-19 0.3856481884221452 1104 20-21 0.39336363090146165 1104 22-23 0.41489491457161876 1104 24-25 0.29143048865618226 1104 26-27 0.27993809985969165 1104 28-29 0.15267013343518698 1104 30-31 0.26290087199640055 1104 32-33 0.12130511913404973 1104 34-35 0.2767624205046104 1104 36-37 0.1748015862404344 1104 38-39 0.08562496107328599 1104 40-41 0.31014313613861333 1104 42-43 0.399160366914451 1104 44-45 0.47795630603236106 1104 46-47 0.47115381185175664 1104 48-49 0.3963232444988236 1104 50-51 0.18910964908475592 1104 52-53 0.38624738492827504 1104 54-55 0.2903716420211424 1104 56-57 0.45188792522576193 1104 58-59 0.5565275008710131 1104 60-61 0.6293419062723657 1104 62-63 0.5083561341611826 1104 64-65 0.48414678543265666 1104 66-67 0.6150112264079866 1104 68-69 0.7239449254240498 1104 70-71 0.3746297115078292 1104 72-73 0.49412031959134595 1104 74-75 0.45814261122731637 1104 76-77 0.3113764649260311 1104 78-79 0.2227150993213911 1104 80-81 0.40847841920398764 1104 82-83 0.5790372102447705 1104 84-85 0.8007800983302538 1104 86-87 0.7297006760847786 1104 88-89 0.6946911829838562 1104 90-91 0.7224668706281747 1104 92-93 0.653779021756062 1104 94-95 0.5573197966086454 1104 96-97 0.8100964380088449 1104 98-99 1.0869857440484267 1104 100-101 1.1419414473541174 1105 1 0.03034659620269764 1105 2 -0.11146087853991204 1105 3 -0.06730004147064506 1105 4 -0.15416040820446142 1105 5 -0.18073761700839697 1105 6 -0.3473879037652523 1105 7 -0.28670604352289075 1105 8 -0.20475430014475648 1105 9 -0.16391397940754615 1105 10-11 -0.1467070554739891 1105 12-13 -0.1821423004110443 1105 14-15 -0.25631186387875715 1105 16-17 -0.22085398174384352 1105 18-19 -0.12903014721427297 1105 20-21 -0.20530825609378667 1105 22-23 -0.2708720131114717 1105 24-25 -0.15649673382194607 1105 26-27 -0.331531269094711 1105 28-29 -0.19378311391626113 1105 30-31 -0.19376272388874582 1105 32-33 -0.23859431277137588 1105 34-35 -0.40982210275655717 1105 36-37 -0.3072612112415243 1105 38-39 -0.13638486542756567 1105 40-41 -0.22502115067237582 1105 42-43 -0.18763143409890404 1105 44-45 -0.2005328694628119 1105 46-47 -0.22002536824957986 1105 48-49 -0.13349404400110387 1105 50-51 -0.2186210433764728 1105 52-53 -0.19910114877355056 1105 54-55 0.044398204281243636 1105 56-57 0.11814387486494837 1105 58-59 0.3201118705931023 1105 60-61 0.2604028612854137 1105 62-63 0.24317111957497417 1105 64-65 0.19634930347349666 1105 66-67 0.22713697491097662 1105 68-69 0.32021776436156557 1105 70-71 0.24551102580231543 1105 72-73 0.2595279658373215 1105 74-75 0.13272037876915377 1105 76-77 0.17884153478631148 1105 78-79 0.33242260738219187 1105 80-81 0.2051926829841193 1105 82-83 0.28697134198129604 1105 84-85 0.04852691239443274 1105 86-87 0.003449332617865508 1105 88-89 0.16850509362411614 1105 90-91 0.43579022392158606 1105 92-93 0.17221906474700432 1105 94-95 0.02037445651959402 1105 96-97 -0.09827144161651802 1105 98-99 -0.34420955765585504 1105 100-101 0.06852289983377347 1106 1 0.03827793040521854 1106 2 0.01641830442401826 1106 3 -0.05893411361319423 1106 4 -0.2024872226329748 1106 5 -0.19470980301448293 1106 6 -0.018270128884765313 1106 7 -0.0015039574733535233 1106 8 0.08068681241499576 1106 9 -0.07195396202379811 1106 10-11 -0.2647970163605535 1106 12-13 -0.22073421696906337 1106 14-15 -0.09413237669927099 1106 16-17 -0.2765688883062154 1106 18-19 -0.47515139884400526 1106 20-21 -0.3860774868630159 1106 22-23 -0.23113928820926333 1106 24-25 -0.2989565339088571 1106 26-27 6.06931687563872E-4 1106 28-29 -0.16090610392060967 1106 30-31 -0.02785659611821245 1106 32-33 -0.14747088817336618 1106 34-35 -0.1980337498665179 1106 36-37 -0.40646807782127325 1106 38-39 -0.5083861692085279 1106 40-41 -0.18871085080275662 1106 42-43 -0.36476094300807205 1106 44-45 -0.5951982323485083 1106 46-47 -0.5806620479540499 1106 48-49 -0.6276161648181358 1106 50-51 -0.6015632424203687 1106 52-53 -0.5287404360399606 1106 54-55 -0.3580137905036409 1106 56-57 -0.4191877200937668 1106 58-59 -0.6387429751261422 1106 60-61 -0.41634637817568887 1106 62-63 -0.11828042323249832 1106 64-65 -0.2911669937885648 1106 66-67 -0.401252855597491 1106 68-69 -0.3621160035654256 1106 70-71 -0.37219866563618353 1106 72-73 -0.2225776404208304 1106 74-75 -0.3225925286623976 1106 76-77 -0.4656499906374165 1106 78-79 -0.4752914299928612 1106 80-81 -0.46973126312626334 1106 82-83 -0.6457970196006215 1106 84-85 -0.5776790849980067 1106 86-87 -0.601602818620723 1106 88-89 -0.45857009107819025 1106 90-91 -0.21675214895977035 1106 92-93 -0.09834807997268413 1106 94-95 -0.050084039786359114 1106 96-97 -0.027639107848592914 1106 98-99 0.0764771003189395 1106 100-101 0.4654046903596303 1107 1 -0.18279216912224427 1107 2 -0.4924507161588032 1107 3 -0.33223621014447247 1107 4 -0.20707712007745016 1107 5 -0.1851913438175572 1107 6 -0.2855619300210819 1107 7 -0.48311846983659024 1107 8 -0.3220448140502228 1107 9 -0.24124029671975933 1107 10-11 -0.3145029760555218 1107 12-13 -0.3147613738622823 1107 14-15 -0.4551455407801299 1107 16-17 -0.29095813710893026 1107 18-19 -0.37498146904711405 1107 20-21 -0.04898298756823749 1107 22-23 -0.2615124962766373 1107 24-25 -0.034242565799083025 1107 26-27 -0.3322184051838164 1107 28-29 -0.3063504713355272 1107 30-31 -0.23273057315278578 1107 32-33 -0.2292483578330362 1107 34-35 -0.20331691307082878 1107 36-37 -0.2407636162178406 1107 38-39 0.02630002842798973 1107 40-41 -0.12462062266254037 1107 42-43 -0.03276632976271543 1107 44-45 0.09989930297218308 1107 46-47 0.19666118919861475 1107 48-49 -0.1177839431005907 1107 50-51 0.052756122006329065 1107 52-53 0.06466417616948661 1107 54-55 -0.14617627765431251 1107 56-57 -0.2929904421579437 1107 58-59 -0.045058466826880306 1107 60-61 0.03424648357756155 1107 62-63 0.10660463011708288 1107 64-65 0.15237168964398506 1107 66-67 0.20234854049630968 1107 68-69 0.37186417859612675 1107 70-71 0.4026174595660095 1107 72-73 0.22040822333252663 1107 74-75 -0.07841755475946854 1107 76-77 0.010670401735065838 1107 78-79 0.36705336214693673 1107 80-81 0.18487696203370163 1107 82-83 -0.021314976777517813 1107 84-85 0.13975236536177604 1107 86-87 0.27655970837281174 1107 88-89 0.5038401628801985 1107 90-91 0.5667845821726445 1107 92-93 0.4437209995775717 1107 94-95 0.29448298785661464 1107 96-97 0.5342517944328087 1107 98-99 0.5234851007651784 1107 100-101 0.5773372284294851 1108 1 -0.1801914206936992 1108 2 -0.31025293426838374 1108 3 -0.23378144061100414 1108 4 -0.24959541635623594 1108 5 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-1.3498102560821827 2316 96-97 -1.5403543192444218 2316 98-99 -1.621627834481714 2316 100-101 -1.8213452493945645 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3554.0 3 674.0 4 545.0 5 657.0 6 715.0 7 859.0 8 1030.0 9 1242.0 10 1588.0 11 2082.0 12 2831.0 13 3629.0 14 4412.0 15 5360.0 16 6707.0 17 8446.0 18 7567.0 19 5511.0 20 5187.0 21 5207.0 22 5732.0 23 6207.0 24 6924.0 25 7986.0 26 9114.0 27 10814.0 28 13024.0 29 15900.0 30 19420.0 31 23993.0 32 30529.0 33 40326.0 34 54812.0 35 78484.0 36 116190.0 37 169705.0 38 151074.0 39 22400.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.48461351614584 19.43703556967369 14.005249492383992 22.073101421796476 2 14.746087993907715 20.645395538768767 41.830939093654735 22.777577373668787 3 19.844154313164953 25.45778196929062 29.510595031092514 25.18746868645192 4 12.201789762445639 16.77592839064023 37.88727396615932 33.135007880754806 5 13.062559160141124 38.269052365050044 34.1833926064411 14.484995868367728 6 30.60201673662843 36.66082702931325 18.12777377865379 14.609382455404532 7 27.940150718954865 33.52719115103472 20.792546999523996 17.74011113048642 8 25.69354606870778 35.789993331401085 19.950067982006566 18.56639261788457 9 26.11937133297203 17.46059049459224 21.01522184782865 35.40481632460708 10-11 24.76859911663806 27.428346880690757 27.061369322938482 20.741684679732693 12-13 25.707867445667958 24.946167255905696 28.00062684585189 21.34533845257446 14-15 22.446237509218612 26.219036645908584 26.90745806561062 24.42726777926218 16-17 21.24934976338162 29.746049830953208 27.477953812139205 21.52664659352597 18-19 21.14172551181733 28.07573412123397 30.119940436219867 20.66259993072883 20-21 21.968272070149933 27.07035315892063 30.192015767948206 20.76935900298123 22-23 21.61682159861972 27.023898653826784 30.175238730298233 21.18404101725526 24-25 21.505435246787595 27.142953911581152 30.346495141999686 21.005115699631563 26-27 21.42302567322861 27.342972232007472 30.37949629057132 20.85450580419259 28-29 20.979069175632308 27.74229614654169 30.39605511807313 20.882579559752873 30-31 21.260534597308283 27.415637801899496 30.269478327566034 21.05434927322619 32-33 21.050935564407904 27.491902693289695 30.27043649461142 21.186725247690987 34-35 21.592652828968333 27.468540020133915 30.103090597319916 20.835716553577836 36-37 21.524043459949166 27.348960772359522 30.126026784004996 21.00096898368632 38-39 21.130242404322917 27.772712226003716 30.266200635903452 20.83084473376991 40-41 21.38809355660099 27.671280804687136 30.091126329919604 20.849499308792268 42-43 21.17938511576568 27.737584838580666 29.737722665381856 21.3453073802718 44-45 21.317529436436857 27.817403372848965 29.555019098044482 21.310048092669696 46-47 21.489099267001396 28.10576055378975 29.178398335614876 21.22674184359398 48-49 21.466903080249057 28.147785978946626 29.066323434987247 21.31898750581707 50-51 21.402377060045065 28.341919615900085 29.073653514779302 21.18204980927554 52-53 21.613427931969575 28.60988195593658 28.603106956039674 21.173583156054168 54-55 21.30645433047765 28.380131421899176 28.74144149345424 21.57197275416894 56-57 21.394961754639535 28.706313208505097 28.609956221180514 21.288768815674857 58-59 21.758365759442345 28.274408806316746 28.653510117711633 21.313715316529276 60-61 21.463014127859275 28.356846389936457 29.137774751730277 21.04236473047399 62-63 21.326387273278772 28.41425454031434 29.113847259319904 21.14551092708698 64-65 21.26360316139326 28.496182545193683 28.990864376841657 21.2493499165714 66-67 21.173076458658887 28.557477259487758 28.683762717634785 21.58568356421857 68-69 21.065868164721653 28.532093016681138 28.89726158909583 21.50477722950138 70-71 21.28471265098987 28.541532669413755 28.642958282384928 21.530796397211443 72-73 21.254517340402032 28.28916869155438 28.815551137618733 21.64076283042486 74-75 20.96308220231017 28.862191578626213 28.63242350831871 21.542302710744913 76-77 21.138544692862478 28.490536474568763 28.619830874678087 21.751087957890668 78-79 21.112287861045598 28.493887052425976 28.570107462531837 21.82371762399659 80-81 21.235974445824596 28.709457601024337 28.464152123556218 21.590415829594857 82-83 21.25113835706653 28.663624764523547 28.505167817099448 21.580069061310468 84-85 21.177272992315526 28.479143193200297 28.42077487912576 21.922808935358418 86-87 21.167281761200393 28.436862794064467 28.54223184489487 21.85362359984027 88-89 21.21835400167043 28.210012451657057 28.35644016902211 22.215193377650394 90-91 21.257117344970432 27.964134530262598 28.744207908049823 22.034540216717154 92-93 21.350200830180544 27.915836868992987 28.690530675860266 22.043431624966203 94-95 21.014899948401506 27.868251847767954 28.82270616425285 22.294142039577693 96-97 20.726118558647062 28.02105217280168 28.903566041591944 22.349263226959316 98-99 20.924443795221457 28.18920975687908 28.49897158366691 22.38737486423255 100-101 20.748966394572246 28.22443667031815 28.768036561951543 22.258560373158062 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1267.0 1 749.5 2 270.5 3 332.5 4 307.5 5 295.5 6 463.0 7 909.0 8 1436.5 9 1669.0 10 1551.5 11 1375.5 12 1364.5 13 1441.5 14 1554.5 15 1619.5 16 1703.5 17 1789.0 18 1833.0 19 1966.5 20 2179.0 21 2447.0 22 2874.5 23 3434.0 24 4108.0 25 4946.5 26 6056.5 27 7554.0 28 8834.0 29 10303.0 30 12035.5 31 13379.5 32 14944.0 33 16667.5 34 18772.0 35 20628.5 36 22750.0 37 25150.0 38 26684.5 39 27511.0 40 27735.0 41 28389.0 42 29389.0 43 30291.0 44 31313.0 45 31977.0 46 31978.0 47 32894.0 48 36437.5 49 35675.5 50 31021.0 51 28270.5 52 26135.5 53 24693.5 54 23075.0 55 20787.0 56 18262.5 57 16102.0 58 14317.0 59 12669.5 60 10631.5 61 8389.5 62 6482.0 63 4852.5 64 3605.5 65 2731.0 66 2042.5 67 1573.5 68 1170.0 69 808.5 70 560.5 71 424.5 72 316.5 73 245.5 74 204.5 75 151.0 76 107.5 77 71.5 78 40.5 79 26.0 80 18.5 81 14.5 82 10.5 83 6.0 84 3.0 85 4.0 86 4.0 87 1.0 88 4.0 89 4.5 90 0.5 91 1.0 92 2.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27820631008597824 2 0.2542189528342709 3 0.25551238876451 4 0.2562178992719132 5 0.24869245385961283 6 0.19413297462043533 7 0.2017760051173029 8 0.19918913325682464 9 0.19613192105807764 10-11 0.20677337121140857 12-13 0.2063030308731398 14-15 0.19072300716798676 16-17 0.20206996782872083 18-19 0.18931198615318043 20-21 0.19254557597877822 22-23 0.19219282072507668 24-25 0.18701907700412018 26-27 0.19577916580437604 28-29 0.19425055970500255 30-31 0.19954188851052623 32-33 0.2009529095253325 34-35 0.19184006547137508 36-37 0.19054662954113596 38-39 0.18543167836246308 40-41 0.18478496039734352 42-43 0.1830211841288356 44-45 0.19636709122721202 46-47 0.18884164581491167 48-49 0.19436814478956974 50-51 0.2011292871521833 52-53 0.20453925460463193 54-55 0.20353978138581078 56-57 0.17890570616898388 58-59 0.17243852651778827 60-61 0.17902329125355107 62-63 0.17937604650725264 64-65 0.17855295091528228 66-67 0.18549047090474666 68-69 0.17931725396496906 70-71 0.18225688107914886 72-73 0.19231040580964387 74-75 0.1820805034522981 76-77 0.18931198615318043 78-79 0.18743062480010536 80-81 0.17714192990047598 82-83 0.19248678343649464 84-85 0.17972880176095424 86-87 0.17955242413410344 88-89 0.18402065734765677 90-91 0.18266842887513404 92-93 0.18772458751152332 94-95 0.1864899441235678 96-97 0.17749468515417754 98-99 0.18460858277049272 100-101 0.173790754990311 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 850448.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.40400487987276 #Duplication Level Percentage of deduplicated Percentage of total 1 85.5263364549592 58.50343936222669 2 8.847867169787344 12.104590981171988 3 2.3209668102537817 4.762902750438672 4 1.0189661397028553 2.788054591706369 5 0.558021024439868 1.9085436439428167 6 0.35526632123463797 1.4580983502833171 7 0.2544756818253872 1.2185009046974915 8 0.1616793238648935 0.8847610606902965 9 0.12291103282509547 0.756684620024223 >10 0.7765123839652287 9.667555972267062 >50 0.03845779625653291 1.7908096362945505 >100 0.016479876398438693 1.996007026794555 >500 0.0012016576171925762 0.5629401995553551 >1k 8.583268694232687E-4 1.59711089990663 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4396 0.5169040317573796 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3723 0.4377692698436589 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2416 0.28408556431433785 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1911 0.2247050966079055 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1155 0.13581077267510772 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 897 0.10547382085677197 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 862 0.10135834289692021 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.1758508456719281E-4 0.0 18-19 0.0 0.0 0.0 1.1758508456719281E-4 0.0 20-21 0.0 0.0 0.0 1.7637762685078923E-4 0.0 22-23 0.0 0.0 0.0 5.291328805523676E-4 0.0 24-25 0.0 0.0 0.0 8.230955919703497E-4 0.0 26-27 0.0 0.0 0.0 0.0010582657611047353 0.0 28-29 0.0 0.0 0.0 0.001998946437642278 0.0 30-31 0.0 0.0 0.0 0.004174270502135345 0.0 32-33 0.0 0.0 0.0 0.0076430304968675335 0.0 34-35 0.0 0.0 0.0 0.011405753203017704 0.0 36-37 1.1758508456719281E-4 0.0 0.0 0.015286060993735067 0.0 38-39 1.1758508456719281E-4 0.0 0.0 0.021106522679811113 0.0 40-41 1.1758508456719281E-4 0.0 0.0 0.02751490978872312 0.0 42-43 1.1758508456719281E-4 0.0 0.0 0.04121357214080108 0.0 44-45 1.1758508456719281E-4 0.0 0.0 0.05614687788083457 0.0 46-47 1.1758508456719281E-4 0.0 0.0 0.07419618836189867 0.0 48-49 1.1758508456719281E-4 0.0 0.0 0.09788958290218802 0.0 50-51 1.1758508456719281E-4 0.0 0.0 0.127756194382255 0.0 52-53 1.1758508456719281E-4 0.0 0.0 0.1769067597313416 0.0 54-55 1.1758508456719281E-4 0.0 0.0 0.20930145052960322 0.0 56-57 1.1758508456719281E-4 0.0 0.0 0.28949447820442875 0.0 58-59 1.1758508456719281E-4 0.0 0.0 0.40355201023460574 0.0 60-61 1.1758508456719281E-4 0.0 0.0 0.5118478731209903 0.0 62-63 1.1758508456719281E-4 0.0 0.0 0.6928701108121837 0.0 64-65 1.1758508456719281E-4 0.0 0.0 0.807750738434331 0.0 66-67 1.1758508456719281E-4 0.0 0.0 1.0299865482663255 0.0 68-69 1.1758508456719281E-4 0.0 0.0 1.2994327695520478 0.0 70-71 1.1758508456719281E-4 0.0 0.0 1.5079699170319643 0.0 72-73 1.1758508456719281E-4 0.0 0.0 1.8148669877523376 0.0 74-75 1.1758508456719281E-4 0.0 0.0 2.1390490659040884 0.0 76-77 1.1758508456719281E-4 0.0 0.0 2.3604029876018284 0.0 78-79 1.1758508456719281E-4 0.0 0.0 2.6045684157056046 0.0 80-81 2.3517016913438563E-4 0.0 0.0 3.0184091208398396 0.0 82-83 2.3517016913438563E-4 0.0 0.0 3.362169115572028 0.0 84-85 2.3517016913438563E-4 0.0 0.0 3.8186344138618704 0.0 86-87 2.3517016913438563E-4 0.0 0.0 4.429782890899855 0.0 88-89 2.3517016913438563E-4 0.0 0.0 5.250409196094294 5.879254228359641E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 725 0.0 32.135414 2 TACATGG 4205 0.0 26.119917 2 GTACATG 4355 0.0 25.98911 1 ACATGGG 4175 0.0 25.282633 3 GTACCTG 945 0.0 25.161753 1 GTATCAA 7375 0.0 23.600529 1 GAGTACT 3605 0.0 22.726015 12-13 ACCTGGG 1055 0.0 22.08358 3 GTCTAGA 290 1.03682396E-10 21.318075 1 CTACACG 135 3.7708966E-4 21.130886 4 CATGGGG 1575 0.0 21.130884 4 CATGGGA 2690 0.0 21.032694 4 TCAACGC 8240 0.0 20.887264 4 GTACTTT 3875 0.0 20.836113 14-15 ATCAACG 8285 0.0 20.775042 3 CAACGCA 8285 0.0 20.770142 5 TATCAAC 8375 0.0 20.551786 2 AACGCAG 8410 0.0 20.50224 6 GGTATCA 5550 0.0 20.050516 1 AGTACTT 3790 0.0 19.736986 12-13 >>END_MODULE