FastQCFastQC Report
Thu 26 May 2016
SRR937257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850448
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60410.7103314958704118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53840.6330780953097662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.40178823396609786No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16940.19918913325682464No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13230.1555650668823961No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10810.12710947641713544No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9400.11052997949316123No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA1303.0363844E-729.2295766
TACCTGG6200.028.348952
CGTCGCA700.00839203627.1417489
GGTATCA49650.027.0047421
GTATCAA68300.025.6175331
GTACATG40400.024.3612291
TACATGG39450.024.2033792
AAGTGCG1050.002196276322.6181249
ACATGGG40200.022.4519733
GTACCTG8100.022.3053341
AGTACTT50800.021.55177712-13
GAGTACT48950.021.5415112-13
ATCAACG81750.021.093363
TCAACGC81950.021.0418854
TATCAAC82650.021.0360972
CAACGCA82550.020.8889455
AACGCAG85250.020.2806976
ATGGGAG11350.020.0896475
TCGCAAG1200.00476038419.7931865
GTACTTT52650.019.75702714-15
Produced by FastQC (version 0.11.5)