##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937257_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 850448 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16511767915264 33.0 31.0 34.0 31.0 34.0 2 32.38077460350309 34.0 31.0 34.0 31.0 34.0 3 32.46373323236694 34.0 31.0 34.0 31.0 34.0 4 36.07381286098621 37.0 35.0 37.0 35.0 37.0 5 35.97717085583128 37.0 35.0 37.0 35.0 37.0 6 36.0573533008485 37.0 35.0 37.0 35.0 37.0 7 36.015872810565725 37.0 35.0 37.0 35.0 37.0 8 36.003402912347376 37.0 35.0 37.0 35.0 37.0 9 37.69378021936674 39.0 37.0 39.0 35.0 39.0 10-11 37.63176702161684 39.0 37.0 39.0 35.0 39.0 12-13 37.55557894192238 39.0 37.0 39.0 35.0 39.0 14-15 38.75809690892329 40.0 38.0 41.0 35.0 41.0 16-17 38.73676227117942 40.0 38.0 41.0 35.0 41.0 18-19 38.76730499689575 40.0 38.0 41.0 35.0 41.0 20-21 38.67142905856678 40.0 38.0 41.0 34.0 41.0 22-23 38.554407794480085 40.0 38.0 41.0 34.0 41.0 24-25 38.41945304122063 40.0 38.0 41.0 34.0 41.0 26-27 38.35003433484469 40.0 38.0 41.0 34.0 41.0 28-29 38.25562644629654 40.0 38.0 41.0 34.0 41.0 30-31 38.305954626267564 40.0 38.0 41.0 34.0 41.0 32-33 38.20319760878972 40.0 37.5 41.0 34.0 41.0 34-35 38.06864734822118 40.0 37.0 41.0 34.0 41.0 36-37 37.897422299776125 40.0 37.0 41.0 33.0 41.0 38-39 37.765084402573706 40.0 37.0 41.0 33.0 41.0 40-41 37.58115487366659 40.0 37.0 41.0 33.0 41.0 42-43 37.3448905753203 40.0 37.0 41.0 33.0 41.0 44-45 37.02586460312682 40.0 36.5 41.0 31.5 41.0 46-47 36.643768343273194 40.0 36.0 41.0 31.0 41.0 48-49 36.302649309540385 39.5 36.0 41.0 30.0 41.0 50-51 36.05011241134085 39.0 36.0 41.0 29.5 41.0 52-53 35.80144112279646 39.0 35.0 41.0 28.5 41.0 54-55 35.57058279871315 39.0 35.0 40.0 28.0 41.0 56-57 35.33297744247737 39.0 35.0 40.0 27.5 41.0 58-59 35.10287460256241 38.0 35.0 40.0 27.0 41.0 60-61 34.77918167836246 38.0 34.0 40.0 26.0 41.0 62-63 34.422050495738716 38.0 34.0 40.0 26.0 41.0 64-65 34.08636800839088 37.0 34.0 40.0 25.5 41.0 66-67 33.670044494196 37.0 33.5 39.5 24.0 41.0 68-69 33.2832401275563 36.0 33.0 39.0 23.0 40.5 70-71 32.87408989144545 36.0 32.5 39.0 22.0 40.0 72-73 32.43657989671326 35.0 32.0 38.0 21.5 40.0 74-75 31.995622895226987 35.0 32.0 37.0 20.0 39.5 76-77 30.634680192086996 34.0 30.0 36.0 18.5 38.5 78-79 31.27247286136248 35.0 31.0 36.0 18.5 39.0 80-81 31.123836495588208 35.0 31.0 36.0 18.0 38.0 82-83 30.785818180535436 34.5 31.0 35.5 16.5 37.0 84-85 30.411394347449814 34.0 31.0 35.0 10.0 37.0 86-87 30.03084433145824 34.0 31.0 35.0 7.0 36.0 88-89 29.633029885425096 34.0 30.0 35.0 2.0 36.0 90-91 29.201076373864126 34.0 29.5 35.0 2.0 35.5 92-93 28.844642470791868 34.0 29.0 35.0 2.0 35.0 94-95 28.446606964799727 34.0 29.0 35.0 2.0 35.0 96-97 27.99212650273738 34.0 29.0 35.0 2.0 35.0 98-99 27.52065852350761 34.0 28.5 35.0 2.0 35.0 100-101 25.651956968562452 31.5 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20442503412306934 1101 2 0.1508900097521817 1101 3 0.14041333230054676 1101 4 0.04038888780349481 1101 5 0.04347409284605419 1101 6 0.07725899381041046 1101 7 0.09563197903590037 1101 8 0.1933941083884605 1101 9 0.20673032386146417 1101 10-11 0.08301726453877478 1101 12-13 0.030750911785659696 1101 14-15 0.09604800136049363 1101 16-17 0.049311261577400956 1101 18-19 9.387459985106261E-4 1101 20-21 -0.02418534105629533 1101 22-23 -0.03151566611905565 1101 24-25 0.0326582752493394 1101 26-27 0.22192895567319937 1101 28-29 0.14176224069385768 1101 30-31 0.1608020125271139 1101 32-33 0.23607104899523534 1101 34-35 0.15378335043111235 1101 36-37 0.25121005443990185 1101 38-39 0.12847305941736664 1101 40-41 0.10960450627909779 1101 42-43 0.15282047237799645 1101 44-45 0.25208753508012194 1101 46-47 0.21908525599299367 1101 48-49 0.2839423795360645 1101 50-51 0.2713178607635385 1101 52-53 0.2833648581347816 1101 54-55 0.1733105276704805 1101 56-57 0.46961454488408805 1101 58-59 0.28743938574643124 1101 60-61 0.27301860964363556 1101 62-63 0.32940156710362345 1101 64-65 0.09656350076528497 1101 66-67 0.32978672473850423 1101 68-69 0.19133742886168648 1101 70-71 0.13551636660729827 1101 72-73 -0.059378766778799275 1101 74-75 0.023085819689363518 1101 76-77 0.04965408567926133 1101 78-79 0.22166343870384964 1101 80-81 0.1664735096095491 1101 82-83 0.24088155325977567 1101 84-85 0.28210809243516266 1101 86-87 0.40108664509456204 1101 88-89 0.47481737909403776 1101 90-91 0.4277004092728127 1101 92-93 0.3548518463286676 1101 94-95 0.24243424722021345 1101 96-97 0.3620905037526505 1101 98-99 0.4821143178112237 1101 100-101 0.7601768986166135 1102 1 0.1486633250853373 1102 2 0.10872736552399687 1102 3 0.14201355794622117 1102 4 0.12281640483696776 1102 5 0.0554541825499939 1102 6 0.08098524517382089 1102 7 0.08064463899732743 1102 8 0.13683269412484833 1102 9 0.20221170401736543 1102 10-11 0.1932844687986517 1102 12-13 0.09742358371413218 1102 14-15 0.1236912268545396 1102 16-17 0.03762382648721285 1102 18-19 0.1972663789525626 1102 20-21 0.06979918004174124 1102 22-23 0.14449937257820267 1102 24-25 0.17539506054845333 1102 26-27 0.04749213238604 1102 28-29 0.04059260047154112 1102 30-31 0.15467705308301305 1102 32-33 0.25273768848877154 1102 34-35 0.20574870142915813 1102 36-37 0.19757518631381998 1102 38-39 0.20008132287499336 1102 40-41 0.3983563791871063 1102 42-43 0.47371177279163135 1102 44-45 0.33005471863407365 1102 46-47 0.08906794127434381 1102 48-49 0.2268832893902939 1102 50-51 0.23873048420427523 1102 52-53 0.18106454395904592 1102 54-55 0.13695654764925536 1102 56-57 0.17340242490710978 1102 58-59 0.15425096671719274 1102 60-61 0.06262480576950935 1102 62-63 0.056861047890322425 1102 64-65 0.3212814844320633 1102 66-67 0.2697297589880563 1102 68-69 0.3375893303014834 1102 70-71 0.3263638585001942 1102 72-73 0.35321002821786607 1102 74-75 0.19693611095814845 1102 76-77 0.14889864548269927 1102 78-79 0.1503192980731214 1102 80-81 0.2114273777861726 1102 82-83 0.3605756598969201 1102 84-85 0.1811467342568882 1102 86-87 0.22224247593204538 1102 88-89 0.19080382078892555 1102 90-91 0.27068591023392585 1102 92-93 0.5070844178904643 1102 94-95 0.4770331962722203 1102 96-97 0.39437503577428146 1102 98-99 0.3766645019635284 1102 100-101 0.8915184958423445 1103 1 0.1326995482679294 1103 2 0.1028381389434685 1103 3 0.08318048034779224 1103 4 0.12532741967257977 1103 5 -0.0013288307933621013 1103 6 -0.04319161277538086 1103 7 0.05794237451033979 1103 8 0.12711327312899812 1103 9 0.04518090731719582 1103 10-11 0.08801913972567377 1103 12-13 0.1175072184439756 1103 14-15 0.01045521926051407 1103 16-17 0.03780901383133539 1103 18-19 0.01127416489213573 1103 20-21 0.02869162520521229 1103 22-23 0.1737024751163858 1103 24-25 0.15896450400892093 1103 26-27 0.16022952417699088 1103 28-29 0.1090827720904386 1103 30-31 0.1161688469968496 1103 32-33 0.09584589439557334 1103 34-35 0.1217812621172385 1103 36-37 0.13713012687002646 1103 38-39 0.053368106776161994 1103 40-41 0.13557419268506976 1103 42-43 0.04826021609694919 1103 44-45 -0.016542901103221652 1103 46-47 -0.05855103374733517 1103 48-49 0.05377392662308722 1103 50-51 -0.29824331245912816 1103 52-53 -0.1362053192892958 1103 54-55 -0.01039294167310345 1103 56-57 -0.16403671661279162 1103 58-59 -0.14848415619249522 1103 60-61 -0.4687669152352214 1103 62-63 -0.38766659883140875 1103 64-65 -0.4001272486981762 1103 66-67 -0.4576744704577109 1103 68-69 -0.13014188708334729 1103 70-71 -0.4855100132246122 1103 72-73 -0.21434479037475995 1103 74-75 -0.26025781202723763 1103 76-77 -0.3253217516701703 1103 78-79 -0.4902293766042831 1103 80-81 -0.2832376124791587 1103 82-83 -0.4359518756227274 1103 84-85 -0.35874611715290783 1103 86-87 -0.48981093335351034 1103 88-89 -0.48262166081027047 1103 90-91 -0.42487685751423854 1103 92-93 -0.3888712340003728 1103 94-95 -0.5178003907035027 1103 96-97 -0.2635399968644272 1103 98-99 -0.09315130613036615 1103 100-101 0.008196387343186728 1104 1 0.23003585753689748 1104 2 0.13440132334682886 1104 3 0.14111037841629326 1104 4 0.09335228509902294 1104 5 0.0753081716555073 1104 6 0.020680642713642783 1104 7 0.08280494679189587 1104 8 0.07481843477901151 1104 9 0.19371130124811486 1104 10-11 0.07645932322902382 1104 12-13 0.22717349123681174 1104 14-15 0.15884528374546392 1104 16-17 0.11406736462261335 1104 18-19 -0.019439907439846138 1104 20-21 -0.008824381865487396 1104 22-23 -0.06011444775855068 1104 24-25 0.07759958846346393 1104 26-27 0.09931370091408809 1104 28-29 -0.12913098152720437 1104 30-31 0.08758968501575737 1104 32-33 0.1574034275656473 1104 34-35 0.05013190116326882 1104 36-37 -0.007751225772636872 1104 38-39 0.012026576300414149 1104 40-41 0.15647091211560848 1104 42-43 0.3427793760611024 1104 44-45 0.29755293011327666 1104 46-47 0.4181247852533687 1104 48-49 0.5460239356612462 1104 50-51 0.3089531620846415 1104 52-53 0.38128686507410947 1104 54-55 0.4306372952791122 1104 56-57 0.3786067857057347 1104 58-59 0.5803475036527388 1104 60-61 0.474214138862493 1104 62-63 0.3509243793647201 1104 64-65 0.21052275729555703 1104 66-67 0.5096877545679277 1104 68-69 0.4976511445844878 1104 70-71 0.4408493747677582 1104 72-73 0.41392678848848163 1104 74-75 0.49181906377312146 1104 76-77 0.5355618196048226 1104 78-79 0.6570508960249128 1104 80-81 0.5522729678853722 1104 82-83 0.706847368596172 1104 84-85 0.6851598744863097 1104 86-87 0.6553701059025805 1104 88-89 0.6089088149398485 1104 90-91 0.5348457373341944 1104 92-93 0.5252761623413598 1104 94-95 0.20618871402317396 1104 96-97 0.6127365254008623 1104 98-99 0.7630671435289429 1104 100-101 1.2615000754864418 1105 1 -0.07057829876588073 1105 2 -0.045239395216043476 1105 3 -0.1484259335751119 1105 4 0.00774657344540941 1105 5 -0.07815413450141051 1105 6 -0.006012125595894702 1105 7 -0.04632909282118902 1105 8 -0.03691962755785738 1105 9 -0.03609063334423723 1105 10-11 -0.043876927346751415 1105 12-13 0.01703607376406069 1105 14-15 -0.11999319314690382 1105 16-17 -0.26599558639842513 1105 18-19 -0.1043316623914805 1105 20-21 -0.06637082743588252 1105 22-23 -0.06287815112105477 1105 24-25 -0.11427435595538782 1105 26-27 -0.1212797276134765 1105 28-29 -0.035875185427102 1105 30-31 -0.13697272861850962 1105 32-33 -0.04881874347687898 1105 34-35 -0.1446324163555346 1105 36-37 -0.17986469114958226 1105 38-39 -0.26752969577083263 1105 40-41 -0.09242744501628408 1105 42-43 -0.43727973648304186 1105 44-45 -0.25015549918197877 1105 46-47 -0.333844353392422 1105 48-49 -0.21830509857796443 1105 50-51 -0.37384278448376307 1105 52-53 -0.24443476858166235 1105 54-55 -0.4082541525943739 1105 56-57 -0.3515559657145033 1105 58-59 -0.11516915533697869 1105 60-61 -0.1888098132333127 1105 62-63 -0.32359581102372914 1105 64-65 -0.006186132616925022 1105 66-67 -0.199071649700457 1105 68-69 -0.32037434290207756 1105 70-71 -0.2789072657972653 1105 72-73 -0.07758633910248847 1105 74-75 -0.1350042127507578 1105 76-77 -0.26954335466203005 1105 78-79 -0.08175610535693778 1105 80-81 -0.23355537542800064 1105 82-83 -0.12754154588256128 1105 84-85 -0.3120649067205079 1105 86-87 -0.009960770805538033 1105 88-89 -0.040588344587249026 1105 90-91 -0.11719249389565434 1105 92-93 -0.057216544552545656 1105 94-95 -0.3081556741236078 1105 96-97 -0.3798705681044332 1105 98-99 -0.3297740970667924 1105 100-101 0.11778366215925118 1106 1 -0.2102784291439761 1106 2 -0.3051264008657668 1106 3 -0.17287182666507306 1106 4 -0.1204150953942289 1106 5 -0.27502506018589656 1106 6 -0.279067318990073 1106 7 -0.19611179599372264 1106 8 -0.21803653325103056 1106 9 -0.3383939797153843 1106 10-11 -0.5149764492937337 1106 12-13 -0.3073337654362831 1106 14-15 -0.30251166337723845 1106 16-17 -0.24461357857573773 1106 18-19 -0.22979885057053906 1106 20-21 -0.05709225290307529 1106 22-23 -0.16723495251175535 1106 24-25 -0.2793221612574328 1106 26-27 -0.24358524695289674 1106 28-29 -0.20064050485343188 1106 30-31 -0.2662534761196014 1106 32-33 -0.2990903645112084 1106 34-35 -0.5091478444302879 1106 36-37 -0.3098842478640478 1106 38-39 -0.46076089959525746 1106 40-41 -0.5369515649641272 1106 42-43 -0.6914952949358906 1106 44-45 -0.4648121701945769 1106 46-47 -0.29325330602171107 1106 48-49 -0.0183811524675761 1106 50-51 -0.21343426644812524 1106 52-53 -0.23279852347084073 1106 54-55 -0.22489908957829385 1106 56-57 -0.1653434494172359 1106 58-59 -0.5081830623339343 1106 60-61 -0.43753862679263733 1106 62-63 -0.6625571321884465 1106 64-65 -0.3932569713826837 1106 66-67 -0.3539282337943277 1106 68-69 -0.06815791526192072 1106 70-71 -0.18006980382421744 1106 72-73 -0.314624583344127 1106 74-75 -0.42036927142090263 1106 76-77 -0.36051467148080363 1106 78-79 -0.5133836585946625 1106 80-81 -0.18186046017376256 1106 82-83 -0.44626166756878405 1106 84-85 -0.36303187760273303 1106 86-87 -0.35732061435182416 1106 88-89 -0.49344579786842857 1106 90-91 -0.7121729371811831 1106 92-93 -0.6804564402500688 1106 94-95 -0.9270279035890603 1106 96-97 -0.5701899944408133 1106 98-99 -0.3597719240985171 1106 100-101 -0.08514984501154288 1107 1 -0.13403712484812047 1107 2 -0.03583648031242603 1107 3 -0.04773337272740008 1107 4 -0.13867308587975202 1107 5 -0.0982980714295536 1107 6 -0.019214179771147144 1107 7 -0.0872371974105306 1107 8 -0.12603937006264943 1107 9 0.07721087473864685 1107 10-11 -0.20202672301417834 1107 12-13 -0.09893170316986755 1107 14-15 -0.14094089847402103 1107 16-17 -0.20086412641506257 1107 18-19 -0.11065873916989943 1107 20-21 -0.09180932875590742 1107 22-23 -0.03659519571257164 1107 24-25 -0.21040198244267572 1107 26-27 -0.3332675399891585 1107 28-29 -0.31921484764546193 1107 30-31 -0.09777794361977499 1107 32-33 -0.19881783935044695 1107 34-35 -0.182723262979529 1107 36-37 -0.32338300875111514 1107 38-39 -0.07750944333872667 1107 40-41 0.0920785403007045 1107 42-43 -0.20508469832890341 1107 44-45 0.1289022202495076 1107 46-47 0.2845428658763254 1107 48-49 0.44948358899210206 1107 50-51 0.4482739563212661 1107 52-53 0.3418650035784765 1107 54-55 0.555287853119701 1107 56-57 0.562917290225613 1107 58-59 0.4324620334084557 1107 60-61 0.3060068299260692 1107 62-63 0.39631666953754774 1107 64-65 0.3490623213268691 1107 66-67 0.14776209953102892 1107 68-69 0.22564930904540859 1107 70-71 0.15468193531661 1107 72-73 0.183835490126107 1107 74-75 0.20649338227292446 1107 76-77 0.19273829880439308 1107 78-79 0.3078663314446217 1107 80-81 0.2540562841864791 1107 82-83 0.3156115046400245 1107 84-85 0.34636426401472775 1107 86-87 0.27430923751242275 1107 88-89 0.38787626790416496 1107 90-91 0.36403151246939913 1107 92-93 0.2072106009394794 1107 94-95 -0.00951457615246909 1107 96-97 0.14854431743315644 1107 98-99 0.6188344523527576 1107 100-101 1.2222527591177688 1108 1 -0.11376786449879717 1108 2 -0.09437234319292287 1108 3 -0.15683707926949353 1108 4 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2316 92-93 -0.29431096671806145 2316 94-95 -0.27026832450640015 2316 96-97 -0.813392259682999 2316 98-99 -0.9075903566366037 2316 100-101 -1.0419167874701074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 4.0 5 16.0 6 36.0 7 77.0 8 207.0 9 386.0 10 710.0 11 1234.0 12 1954.0 13 2761.0 14 3691.0 15 5221.0 16 7166.0 17 9846.0 18 9023.0 19 5617.0 20 4364.0 21 4109.0 22 4089.0 23 4490.0 24 4987.0 25 6008.0 26 7122.0 27 8738.0 28 10926.0 29 13665.0 30 17382.0 31 21801.0 32 28455.0 33 37241.0 34 51519.0 35 74450.0 36 117001.0 37 190819.0 38 175838.0 39 19489.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55145003247395 19.736631557149444 14.832947411026081 21.87897099935052 2 15.199283201324478 20.807738979925876 41.22932854213309 22.76364927661656 3 20.08447312475307 25.14533516452505 30.15022670404305 24.619965006678832 4 12.559262882621866 17.281009538502058 37.68966474140688 32.47006283746919 5 12.955699842293367 37.90415999755384 34.462838978117475 14.677301182035322 6 30.585173931857092 36.82529678475345 17.76275562997385 14.826773653415612 7 27.539014731059396 33.65132259703121 20.914388651628318 17.895274020281075 8 25.736552969728894 35.61181871202002 19.88516640641168 18.766461911839407 9 25.936565198577693 17.06324196188362 21.26197016160894 35.73822267792975 10-11 24.741547984121308 27.196254209546026 27.03122354335597 21.03097426297669 12-13 25.709192441630414 24.714215245028495 28.30249470409673 21.274097609244357 14-15 22.46321938554738 25.779530318138207 27.55935695068952 24.197893345624895 16-17 21.035266118563392 29.174564464846764 28.192611423626136 21.597557992963708 18-19 20.658993847948377 27.29337948939853 31.432844806501986 20.61478185615111 20-21 21.436936767444923 26.01405376930747 31.84850808044701 20.700501382800596 22-23 21.185575223164022 26.087687601528387 31.719481515145993 21.0072556601616 24-25 21.06506468370714 26.30449199596447 31.93671320695259 20.693730113375803 26-27 21.053021470688357 26.356050437509595 31.960864894992408 20.630063196809644 28-29 20.670455768110287 26.652606971954494 32.0799744597238 20.596962800211426 30-31 20.836664910729404 26.478220890636468 31.84421622486031 20.840897973773824 32-33 20.75553120237804 26.54700816510827 31.724632193855474 20.972828438658215 34-35 21.186010196978533 26.332650555942276 31.665839651571876 20.81549959550731 36-37 21.056901774123755 26.290555095667223 31.824873478449007 20.827669651760015 38-39 20.957483585122194 26.711744868586912 31.756321374146335 20.574450172144566 40-41 21.018392658928 26.662300340526407 31.572300716798672 20.74700628374692 42-43 20.796857727497237 26.879307100689136 31.287426205988194 21.036408965825434 44-45 21.03491336330969 26.852729384982975 30.90330037815363 21.209056873553703 46-47 21.239805373168025 27.157980264519406 30.504099016048013 21.098115346264557 48-49 21.24115760164055 27.34458779372754 30.25822860421801 21.1560260004139 50-51 21.257443135853105 27.45347158203676 30.15698784640566 21.132097435704473 52-53 21.38408227193197 27.76930511918424 29.6640123793577 21.182600229526084 54-55 21.077831919176717 27.552595808326906 29.89036366714955 21.479208605346827 56-57 21.426354109833877 27.74490621413655 29.501215829774424 21.32752384625515 58-59 21.548877767952888 27.429131469531352 29.769662577841327 21.25232818467443 60-61 21.323231990668447 27.667946776287323 30.083085620755178 20.925735612289053 62-63 21.166902620736362 27.618325870599964 30.05045575978778 21.164315748875886 64-65 21.03802936805072 27.597042970293305 29.99942383308562 21.365503828570354 66-67 20.886697364212743 27.783944462212855 29.746968656519858 21.58238951705454 68-69 21.032855624329766 27.63243608074803 29.792180121535942 21.542528173386263 70-71 21.131568302823922 27.677059620341275 29.689116794912803 21.502255281921997 72-73 21.0374785480333 27.41553995460032 29.81145786360459 21.735523633761794 74-75 20.870588207627037 28.054390156717403 29.30843508362651 21.76658655202905 76-77 20.9735927417079 27.627497507196207 29.521146501608563 21.877763249487327 78-79 20.909391285534213 27.699048031155343 29.50227409553553 21.889286587774915 80-81 21.025212593832897 27.9644375670235 29.222362801723328 21.78798703742028 82-83 20.99745310107873 27.788419252956686 29.473593855459374 21.740533790505218 84-85 20.854124078343116 27.78155034138271 29.27730811810613 22.087017462168046 86-87 20.82496519481497 27.618678625853665 29.2056069271725 22.35074925215886 88-89 20.85342078528023 27.47475448234342 29.135349839143604 22.536474893232743 90-91 20.756540825440204 27.190442283686934 29.53130429618619 22.52171259468667 92-93 20.874157716903515 27.06811697218445 29.475689979851644 22.582035331060396 94-95 20.493257671250916 27.059561548736667 29.64760926006058 22.799571519951837 96-97 20.287777735969748 27.21377438714654 29.865259251594455 22.63318862528926 98-99 20.179247229834246 27.344080249704422 29.435983846113594 23.040688674347738 100-101 19.722030796875625 27.520209420635833 29.673097774317746 23.084662008170792 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 458.0 1 325.0 2 298.5 3 416.5 4 344.5 5 313.5 6 530.5 7 1016.5 8 1578.5 9 1884.5 10 1812.5 11 1616.5 12 1570.5 13 1690.0 14 1864.5 15 1957.5 16 2020.0 17 2081.5 18 2194.5 19 2384.0 20 2587.5 21 2859.0 22 3209.0 23 3649.5 24 4313.0 25 5178.5 26 6222.5 27 7660.5 28 8957.5 29 10372.5 30 11971.0 31 13309.5 32 14771.0 33 16445.0 34 18657.0 35 20583.0 36 22562.0 37 24539.5 38 26135.5 39 27050.0 40 27412.0 41 28390.0 42 29244.0 43 29946.5 44 30980.5 45 31867.5 46 31804.0 47 32938.5 48 36872.5 49 35674.5 50 30663.5 51 28011.0 52 25964.0 53 24646.0 54 23112.5 55 20673.5 56 18022.0 57 16001.0 58 14430.0 59 12663.0 60 10625.5 61 8622.0 62 6587.0 63 4762.0 64 3452.0 65 2555.0 66 1921.0 67 1529.0 68 1133.0 69 745.5 70 531.0 71 392.0 72 291.0 73 227.0 74 181.5 75 153.0 76 116.5 77 64.0 78 32.0 79 16.0 80 9.5 81 7.5 82 6.0 83 3.5 84 2.0 85 3.0 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06302560532801535 2 0.0 3 0.0 4 0.0 5 0.01587398641657103 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0031160047410306096 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.99473218821139E-4 24-25 2.3517016913438566E-4 26-27 0.0011170583033883318 28-29 0.0031160047410306096 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012699189133256824 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.7637762685078923E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.3517016913438566E-4 84-85 0.0011170583033883318 86-87 0.0 88-89 0.0 90-91 0.009112844053957445 92-93 7.643030496867533E-4 94-95 0.0 96-97 0.0 98-99 2.939627114179821E-4 100-101 0.011993678625853668 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 850448.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.88044903419444 #Duplication Level Percentage of deduplicated Percentage of total 1 84.4806693344417 55.6562443046232 2 9.320881497832781 12.281277169434771 3 2.573777164148244 5.086847858641256 4 1.1202816164980112 2.9521862375856878 5 0.6064736069106357 1.9977376775330107 6 0.3865324556133096 1.5278959045256804 7 0.26481884167344283 1.221246894151316 8 0.19260332974752198 1.0151035079398214 9 0.15032547703578994 0.8913158935557595 >10 0.8336910054963146 9.958451499368612 >50 0.04615496140536627 2.0625767009044877 >100 0.021107546543556312 2.443758154029383 >500 0.0016098975920132797 0.6733680839497791 >1k 7.155100408947911E-4 0.8856074817785421 >5k 3.5775502044739553E-4 1.3463826319786882 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6041 0.7103314958704118 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5384 0.6330780953097662 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3417 0.40178823396609786 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1694 0.19918913325682464 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1323 0.1555650668823961 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1081 0.12710947641713544 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 940 0.11052997949316123 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.1758508456719281E-4 0.0 2 0.0 0.0 0.0 1.1758508456719281E-4 0.0 3 0.0 0.0 0.0 1.1758508456719281E-4 0.0 4 0.0 0.0 0.0 1.1758508456719281E-4 0.0 5 1.1758508456719281E-4 0.0 0.0 1.1758508456719281E-4 0.0 6 1.1758508456719281E-4 0.0 0.0 1.1758508456719281E-4 0.0 7 1.1758508456719281E-4 0.0 0.0 1.1758508456719281E-4 0.0 8 1.1758508456719281E-4 0.0 0.0 1.1758508456719281E-4 0.0 9 1.1758508456719281E-4 0.0 0.0 1.1758508456719281E-4 0.0 10-11 1.1758508456719281E-4 0.0 0.0 1.1758508456719281E-4 0.0 12-13 1.1758508456719281E-4 0.0 0.0 1.1758508456719281E-4 0.0 14-15 1.1758508456719281E-4 0.0 0.0 1.1758508456719281E-4 0.0 16-17 1.1758508456719281E-4 0.0 0.0 2.3517016913438563E-4 0.0 18-19 1.1758508456719281E-4 0.0 0.0 2.3517016913438563E-4 0.0 20-21 1.1758508456719281E-4 0.0 0.0 2.939627114179821E-4 0.0 22-23 1.1758508456719281E-4 0.0 0.0 5.879254228359642E-4 0.0 24-25 1.1758508456719281E-4 0.0 0.0 8.230955919703497E-4 0.0 26-27 1.1758508456719281E-4 0.0 0.0 9.406806765375425E-4 0.0 28-29 1.1758508456719281E-4 0.0 0.0 0.001998946437642278 5.879254228359641E-5 30-31 1.1758508456719281E-4 0.0 0.0 0.004291855586702538 1.1758508456719281E-4 32-33 1.1758508456719281E-4 0.0 0.0 0.007760615581434726 1.1758508456719281E-4 34-35 1.1758508456719281E-4 0.0 0.0 0.011523338287584898 1.1758508456719281E-4 36-37 1.1758508456719281E-4 0.0 0.0 0.015286060993735067 1.1758508456719281E-4 38-39 1.1758508456719281E-4 0.0 0.0 0.021400485391229095 1.1758508456719281E-4 40-41 1.1758508456719281E-4 0.0 0.0 0.027573702331006714 1.1758508456719281E-4 42-43 1.1758508456719281E-4 0.0 0.0 0.04180149756363705 1.1758508456719281E-4 44-45 1.1758508456719281E-4 0.0 0.0 0.05720514364193931 1.1758508456719281E-4 46-47 1.1758508456719281E-4 0.0 0.0 0.07584237954583936 1.1758508456719281E-4 48-49 1.1758508456719281E-4 0.0 0.0 0.09977094425526312 2.3517016913438563E-4 50-51 1.1758508456719281E-4 0.0 0.0 0.12957876319304648 2.3517016913438563E-4 52-53 1.1758508456719281E-4 0.0 0.0 0.17902329125355107 2.939627114179821E-4 54-55 1.1758508456719281E-4 0.0 0.0 0.21206470001693223 3.5275525370157847E-4 56-57 1.1758508456719281E-4 0.0 0.0 0.2927868605723101 3.5275525370157847E-4 58-59 1.1758508456719281E-4 0.0 0.0 0.40719714785618877 3.5275525370157847E-4 60-61 1.1758508456719281E-4 0.0 0.0 0.5168452392150961 3.5275525370157847E-4 62-63 1.1758508456719281E-4 0.0 0.0 0.6998076308016481 3.5275525370157847E-4 64-65 1.1758508456719281E-4 0.0 0.0 0.8155113540157658 3.5275525370157847E-4 66-67 1.1758508456719281E-4 0.0 0.0 1.0385702594397306 3.5275525370157847E-4 68-69 1.1758508456719281E-4 0.0 0.0 1.3098390495362444 3.5275525370157847E-4 70-71 1.1758508456719281E-4 0.0 0.0 1.5177882715933249 3.5275525370157847E-4 72-73 1.1758508456719281E-4 0.0 0.0 1.8266842887513404 4.7034033826877126E-4 74-75 1.1758508456719281E-4 0.0 0.0 2.1531592760521514 4.7034033826877126E-4 76-77 1.1758508456719281E-4 0.0 0.0 2.3764533516452504 4.7034033826877126E-4 78-79 1.1758508456719281E-4 0.0 0.0 2.6226765187289525 4.7034033826877126E-4 80-81 1.1758508456719281E-4 0.0 0.0 3.044336631986906 4.7034033826877126E-4 82-83 1.1758508456719281E-4 0.0 0.0 3.3912126314601245 4.7034033826877126E-4 84-85 1.1758508456719281E-4 0.0 0.0 3.8508527270332813 4.7034033826877126E-4 86-87 1.1758508456719281E-4 0.0 0.0 4.468409591180178 4.7034033826877126E-4 88-89 1.1758508456719281E-4 0.0 0.0 5.2992657987319625 4.7034033826877126E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGA 130 3.0363844E-7 29.229576 6 TACCTGG 620 0.0 28.34895 2 CGTCGCA 70 0.008392036 27.141748 9 GGTATCA 4965 0.0 27.004742 1 GTATCAA 6830 0.0 25.617533 1 GTACATG 4040 0.0 24.361229 1 TACATGG 3945 0.0 24.203379 2 AAGTGCG 105 0.0021962763 22.618124 9 ACATGGG 4020 0.0 22.451973 3 GTACCTG 810 0.0 22.305334 1 AGTACTT 5080 0.0 21.551777 12-13 GAGTACT 4895 0.0 21.54151 12-13 ATCAACG 8175 0.0 21.09336 3 TCAACGC 8195 0.0 21.041885 4 TATCAAC 8265 0.0 21.036097 2 CAACGCA 8255 0.0 20.888945 5 AACGCAG 8525 0.0 20.280697 6 ATGGGAG 1135 0.0 20.089647 5 TCGCAAG 120 0.004760384 19.793186 5 GTACTTT 5265 0.0 19.757027 14-15 >>END_MODULE