FastQCFastQC Report
Thu 26 May 2016
SRR937256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986923
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49500.5015588855462888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40480.41016371084674286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25760.26101327053883633No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20680.20954015662822734No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13210.1338503611730601No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9930.10061575219140703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG8450.030.9217222
GTACATG49500.026.2061611
GTACCTG10650.025.4314541
TACATGG48600.025.122022
ACATGGG48850.023.631943
GTATCAA81050.023.3919181
GTATAGG4250.022.3607851
ACCTGGG11600.021.705793
GAGTACT38300.021.6415412-13
CATGGGG16550.021.5299514
AGTACTT40200.020.91407812-13
CAACGCA90200.020.7535085
ATCAACG90250.020.7399023
TCAACGC90700.020.638054
TATCAAC91750.020.5561662
AACGCAG91900.020.469886
GTACTTT41450.019.93857814-15
TAGACTG4600.019.6244545
GGTATCA59550.018.9109151
CATGGGA33700.018.8909874
Produced by FastQC (version 0.11.5)