##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937256_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 986923 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81200255744369 33.0 31.0 34.0 30.0 34.0 2 31.883742703331464 33.0 31.0 34.0 30.0 34.0 3 31.933062660410183 33.0 31.0 34.0 30.0 34.0 4 35.53225631584227 37.0 35.0 37.0 33.0 37.0 5 35.556622958427354 37.0 35.0 37.0 33.0 37.0 6 35.59494307053337 37.0 35.0 37.0 33.0 37.0 7 35.484513989439904 37.0 35.0 37.0 33.0 37.0 8 35.49957798126095 37.0 35.0 37.0 33.0 37.0 9 37.13614638629356 39.0 37.0 39.0 34.0 39.0 10-11 37.02889283155829 39.0 37.0 39.0 33.0 39.0 12-13 36.89162781696241 39.0 37.0 39.0 33.0 39.0 14-15 38.08182147948725 40.0 38.0 41.0 33.0 41.0 16-17 38.02137248802592 40.0 38.0 41.0 33.0 41.0 18-19 37.99796640670042 40.0 38.0 41.0 33.0 41.0 20-21 37.898840639036685 40.0 38.0 41.0 33.0 41.0 22-23 37.75022114187226 40.0 37.5 41.0 32.5 41.0 24-25 37.69256264166505 40.0 37.5 41.0 32.5 41.0 26-27 37.71614249541251 40.0 37.5 41.0 33.0 41.0 28-29 37.64160172576787 40.0 37.0 41.0 32.5 41.0 30-31 37.44812969198205 40.0 37.0 41.0 32.0 41.0 32-33 37.22232484195829 40.0 36.5 41.0 31.5 41.0 34-35 37.247413425363476 40.0 37.0 41.0 32.0 41.0 36-37 37.130447866753535 40.0 36.5 41.0 31.0 41.0 38-39 37.01966465468937 40.0 36.0 41.0 31.0 41.0 40-41 36.8229598459049 39.5 36.0 41.0 30.5 41.0 42-43 36.55320374537831 39.0 36.0 41.0 30.0 41.0 44-45 36.3256525585076 39.0 36.0 41.0 30.0 41.0 46-47 36.01561216021919 39.0 35.0 41.0 29.5 41.0 48-49 35.69537846417603 39.0 35.0 41.0 27.5 41.0 50-51 34.711624412441495 38.0 34.0 39.5 25.5 40.5 52-53 34.821968380511954 38.0 34.5 39.5 26.0 40.5 54-55 35.20567663333411 39.0 35.0 40.0 26.5 41.0 56-57 35.00372622788201 38.5 35.0 40.0 26.0 41.0 58-59 34.74539401756773 38.0 34.0 40.0 25.5 41.0 60-61 34.44724208474217 38.0 34.0 40.0 24.5 41.0 62-63 34.15302966898127 38.0 34.0 40.0 23.0 41.0 64-65 33.74661954377393 37.0 33.5 40.0 22.0 41.0 66-67 33.396888612384146 37.0 33.0 39.5 22.0 41.0 68-69 32.93650720471607 36.0 32.5 39.0 20.5 41.0 70-71 32.47455627237383 35.5 32.0 39.0 18.5 40.0 72-73 32.02433573845173 35.0 31.5 38.0 17.5 40.0 74-75 31.542993222368917 35.0 31.0 37.5 12.5 39.5 76-77 31.06169680917356 35.0 31.0 37.0 8.0 39.0 78-79 30.560210877646988 34.5 30.5 36.0 7.0 39.0 80-81 30.061830558209707 34.0 30.0 36.0 2.0 37.5 82-83 29.58374564175726 34.0 29.5 35.5 2.0 37.0 84-85 29.06135230408046 34.0 29.0 35.0 2.0 37.0 86-87 28.617346034087767 34.0 29.0 35.0 2.0 36.0 88-89 28.163756443005177 34.0 27.5 35.0 2.0 36.0 90-91 27.65574365983972 33.5 26.5 35.0 2.0 35.5 92-93 27.152205896508644 33.0 26.0 35.0 2.0 35.0 94-95 26.62388504472993 33.0 25.0 35.0 2.0 35.0 96-97 25.921862191883257 32.5 24.0 35.0 2.0 35.0 98-99 25.028354795662885 32.0 19.0 34.0 2.0 35.0 100-101 22.45615970040216 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.012080318593270079 1101 2 -0.10145374803642682 1101 3 -0.016284069732794393 1101 4 -0.11770482445331965 1101 5 -0.0493001379639324 1101 6 -0.17447961256586098 1101 7 -0.14155596380071245 1101 8 -0.32073158715050454 1101 9 -0.1839446773567559 1101 10-11 -0.13890676800391333 1101 12-13 0.02592281746085945 1101 14-15 -0.1726364876759945 1101 16-17 -0.26374943582571575 1101 18-19 -0.11489945504307997 1101 20-21 -0.10895821524512428 1101 22-23 -0.17261529916492435 1101 24-25 0.06023506453600902 1101 26-27 -0.05177212184256774 1101 28-29 -0.16617077704610494 1101 30-31 -0.18195036700859646 1101 32-33 -0.3415077257626322 1101 34-35 -0.29601790844566267 1101 36-37 -0.337945607267649 1101 38-39 -0.3061508868568481 1101 40-41 -0.2295858352168807 1101 42-43 -0.25385318977954086 1101 44-45 -0.1340270342078398 1101 46-47 -0.2617696863560113 1101 48-49 -0.2062770474561617 1101 50-51 -0.3072284504901788 1101 52-53 -0.22819954728777958 1101 54-55 -0.03296301331695872 1101 56-57 -0.10507120027573791 1101 58-59 -0.13459273252396287 1101 60-61 -0.05287429558227075 1101 62-63 -0.1577333013419704 1101 64-65 -0.2045549602968535 1101 66-67 -0.2866408429865359 1101 68-69 -0.27223063639804224 1101 70-71 -0.4202252374272746 1101 72-73 -0.45845643196139463 1101 74-75 -0.34437141242012004 1101 76-77 -0.39177036159784606 1101 78-79 -0.3566065959050597 1101 80-81 -0.17135839866711322 1101 82-83 -0.133924550349672 1101 84-85 -0.29104472824616323 1101 86-87 -0.21807394032212812 1101 88-89 -0.36966181883694915 1101 90-91 -0.3094614075522557 1101 92-93 -0.11319309275021894 1101 94-95 -0.17448149183692152 1101 96-97 -0.044342296830190975 1101 98-99 0.15402938393658872 1101 100-101 0.44617045058556215 1102 1 -0.27752746352733837 1102 2 -0.2187579635870911 1102 3 -0.08777784956486201 1102 4 -0.2508333079727407 1102 5 -0.11556677182856134 1102 6 -0.3243463678631997 1102 7 -0.21088937099293759 1102 8 -0.2596644021681911 1102 9 -0.3827315450282285 1102 10-11 -0.35054942781473386 1102 12-13 -0.34144806117214443 1102 14-15 -0.21528212410885317 1102 16-17 -0.10969906297406595 1102 18-19 -0.06622915951518138 1102 20-21 -0.2567802739640328 1102 22-23 -0.19255529967542628 1102 24-25 -0.03337972549021373 1102 26-27 -0.3567922456756918 1102 28-29 -0.23823533406381614 1102 30-31 -0.22850129244423556 1102 32-33 -0.34831577790266266 1102 34-35 -0.14199903423984495 1102 36-37 -0.26667910645765147 1102 38-39 -0.1924049321247523 1102 40-41 -0.21113876190269565 1102 42-43 -0.46507170403187104 1102 44-45 -0.28935581761609797 1102 46-47 -0.11339395747463499 1102 48-49 -0.18864975355371172 1102 50-51 -0.20186656634979983 1102 52-53 -0.3550019622563667 1102 54-55 -0.2906238369848282 1102 56-57 -0.16504869503636854 1102 58-59 -0.2558562735474226 1102 60-61 0.06099439525254269 1102 62-63 -0.2215009521240816 1102 64-65 -0.1842117228117317 1102 66-67 -0.3667071422052075 1102 68-69 -0.16110803009146935 1102 70-71 -0.1077116943672749 1102 72-73 -0.12096962201933081 1102 74-75 -0.23572416521168904 1102 76-77 -0.26593218534771523 1102 78-79 -0.03773249870783957 1102 80-81 -0.0496115163542008 1102 82-83 0.03916295234536449 1102 84-85 0.08467437769192898 1102 86-87 0.008464048945523217 1102 88-89 -0.09550901021674818 1102 90-91 -0.059030880802573904 1102 92-93 0.018233377734194534 1102 94-95 0.061897651932135034 1102 96-97 0.16273251625521468 1102 98-99 0.08148610181027749 1102 100-101 0.44193171040105383 1103 1 -0.03221491943170207 1103 2 -0.14616611076125707 1103 3 -0.12650771099874092 1103 4 -0.012323802321873245 1103 5 -0.2790274519135494 1103 6 -0.1786650474793703 1103 7 0.003262354273047663 1103 8 0.004795366199061846 1103 9 0.044847369238624424 1103 10-11 -0.018055243398947596 1103 12-13 -0.14033741273522793 1103 14-15 -0.1809222300132447 1103 16-17 -0.04775034839240533 1103 18-19 -0.21119141509218053 1103 20-21 -0.25066001591854103 1103 22-23 -0.21350032994986634 1103 24-25 -0.32691511113875293 1103 26-27 -0.20375987710444576 1103 28-29 -0.3045545919267312 1103 30-31 -0.39567882053153625 1103 32-33 -0.26902110071007 1103 34-35 -0.298068062157526 1103 36-37 -0.18563039492848077 1103 38-39 -0.35902782712516057 1103 40-41 -0.12123982261687871 1103 42-43 -0.350978264195156 1103 44-45 -0.20992982542472305 1103 46-47 -0.08301699703673648 1103 48-49 -0.04917738643383274 1103 50-51 -0.1509851720148916 1103 52-53 -0.09040367960313489 1103 54-55 0.00883713798296526 1103 56-57 -0.07437280867453211 1103 58-59 -0.04815654948170334 1103 60-61 0.11996231695654558 1103 62-63 0.0982476142098605 1103 64-65 -0.13700714844222972 1103 66-67 -0.08618490015134483 1103 68-69 0.08185034708552763 1103 70-71 0.08107607813294493 1103 72-73 0.03899853985289781 1103 74-75 0.09240638723857231 1103 76-77 0.14676004282015498 1103 78-79 0.12453482752872347 1103 80-81 0.003678759482170335 1103 82-83 0.005819885575718331 1103 84-85 -0.04646351860723641 1103 86-87 0.18202072144649506 1103 88-89 -0.04421034519497269 1103 90-91 -0.1363918294497708 1103 92-93 -0.030553311993227084 1103 94-95 0.04291226763706035 1103 96-97 0.10028618694138558 1103 98-99 0.6471700945695247 1103 100-101 0.8443338533696831 1104 1 0.012202612355171283 1104 2 -0.02696902917247712 1104 3 -0.06491845605822988 1104 4 -0.02860069606162341 1104 5 -0.04196753802089148 1104 6 -4.192921450396625E-4 1104 7 -0.06622579856453115 1104 8 -0.004598862121603986 1104 9 -0.09641249846164612 1104 10-11 0.023241637430622575 1104 12-13 0.04392401247752531 1104 14-15 0.0947761337103401 1104 16-17 -0.06022525349074215 1104 18-19 -0.09971408785466451 1104 20-21 -0.1283334002214218 1104 22-23 -0.004274585393751806 1104 24-25 -0.08728974322824712 1104 26-27 -0.09664388091827902 1104 28-29 0.01638477168432928 1104 30-31 -0.0339592793758996 1104 32-33 -0.02191671184124999 1104 34-35 0.10504755501419538 1104 36-37 -0.039461994073327844 1104 38-39 0.0887842527758238 1104 40-41 0.19269843356135397 1104 42-43 0.2110267222136173 1104 44-45 0.050839810780630046 1104 46-47 0.2590562711929678 1104 48-49 0.21568501355669412 1104 50-51 0.03176315647218786 1104 52-53 0.12205760466361681 1104 54-55 0.03938659048476012 1104 56-57 -0.11234265333782645 1104 58-59 -0.07455431329648121 1104 60-61 0.00745868966987473 1104 62-63 0.2749950780702406 1104 64-65 0.2997554759959158 1104 66-67 0.0775801345817726 1104 68-69 0.08621908076273854 1104 70-71 0.12225481305380015 1104 72-73 0.18890615508217223 1104 74-75 0.2296487018324065 1104 76-77 0.09676892597119036 1104 78-79 0.09603975175544477 1104 80-81 0.06009594778720739 1104 82-83 0.13477718130766192 1104 84-85 0.28404447918974185 1104 86-87 0.4339436275397439 1104 88-89 -0.050647372537316215 1104 90-91 0.35266857005514396 1104 92-93 0.6325623643995009 1104 94-95 0.8780930989815552 1104 96-97 0.8427568281272286 1104 98-99 0.6980255420138448 1104 100-101 0.9976801363631864 1105 1 -0.07654624740765925 1105 2 -0.08340476780611183 1105 3 -0.10290755779139005 1105 4 -0.02477189967197546 1105 5 -0.06894674969692005 1105 6 -0.038186347692594325 1105 7 -0.12651073783190014 1105 8 -0.024958696967253502 1105 9 -0.039202598802518196 1105 10-11 -0.18579564232356915 1105 12-13 -0.06608246887059721 1105 14-15 -0.16476105002388408 1105 16-17 -0.12732821090587976 1105 18-19 -0.13796964501054987 1105 20-21 -0.18152306367141335 1105 22-23 -0.09042670503463768 1105 24-25 -0.23701008505934595 1105 26-27 -0.18461017790804846 1105 28-29 -0.3478429876896172 1105 30-31 -0.29022038168517383 1105 32-33 -0.08801686067220515 1105 34-35 -0.019424935263785414 1105 36-37 0.08411090909053343 1105 38-39 0.1759835902380118 1105 40-41 0.04058239342500514 1105 42-43 -0.1686950599170629 1105 44-45 0.05055835223724614 1105 46-47 0.016901703042798033 1105 48-49 -0.10424573203838605 1105 50-51 -0.13731361149555 1105 52-53 -0.02414180481355288 1105 54-55 -0.1919279229340347 1105 56-57 -0.26653032437340585 1105 58-59 -0.41348381063192363 1105 60-61 -0.2623394283386631 1105 62-63 -0.19851661236136664 1105 64-65 -0.3174393005175986 1105 66-67 -0.37334225727573767 1105 68-69 -0.4215111271205032 1105 70-71 -0.6070060732105418 1105 72-73 -0.49377484256533677 1105 74-75 -0.3445465307759754 1105 76-77 -0.2112325383333875 1105 78-79 -0.12342169622573351 1105 80-81 -0.039800230617903054 1105 82-83 -0.3781603096904682 1105 84-85 -0.3531950666357879 1105 86-87 -0.5175458822783021 1105 88-89 -0.7577528504767237 1105 90-91 -0.515580025652767 1105 92-93 -0.698963993082856 1105 94-95 -0.5347111643052074 1105 96-97 -0.02652278203168379 1105 98-99 -0.09186295479349127 1105 100-101 0.031177903898832682 1106 1 -0.40671871709578866 1106 2 -0.33056809530400244 1106 3 -0.26904741385052233 1106 4 -0.032601115777787015 1106 5 -0.13263409975851914 1106 6 -0.30449316219041833 1106 7 -0.7221228107100686 1106 8 -0.5121288324791493 1106 9 -0.8021783493050165 1106 10-11 -1.1131241452088716 1106 12-13 -0.6382636268813542 1106 14-15 -0.7715009838888847 1106 16-17 -0.58637881714332 1106 18-19 -0.8946479825583751 1106 20-21 -0.721613350904768 1106 22-23 -0.7269071238895961 1106 24-25 -0.541595768778464 1106 26-27 -0.902653035977167 1106 28-29 -0.6499080852711501 1106 30-31 -0.5732919306704645 1106 32-33 -0.5652019089766824 1106 34-35 -0.45827843328622464 1106 36-37 -0.8096516533162657 1106 38-39 -0.6434014191635313 1106 40-41 -0.4754326490440306 1106 42-43 -0.7041453614148239 1106 44-45 -0.6208293479507816 1106 46-47 -0.5152702155203883 1106 48-49 -0.3060182471132791 1106 50-51 -0.5916674985473449 1106 52-53 -0.2739675520741329 1106 54-55 -0.5271010410044497 1106 56-57 -0.3879236979064373 1106 58-59 -0.387252694766012 1106 60-61 -0.5452124102488014 1106 62-63 -0.6422775538124483 1106 64-65 -0.39649331076203964 1106 66-67 -0.5492443191314038 1106 68-69 -0.6280735663346704 1106 70-71 -0.5686137370891657 1106 72-73 -0.7628494389473701 1106 74-75 -0.4018837868221432 1106 76-77 -0.23449730523995527 1106 78-79 -0.2201481761583004 1106 80-81 -0.44781504615314205 1106 82-83 -0.36980428297711754 1106 84-85 -0.6210701557883525 1106 86-87 -0.47901576492110465 1106 88-89 -0.030073410679019474 1106 90-91 -0.29322542538044516 1106 92-93 -0.15832258090623696 1106 94-95 0.06337286603908154 1106 96-97 0.4238597635171715 1106 98-99 0.21292940077935185 1106 100-101 0.5400688459982952 1107 1 -0.06875104854150749 1107 2 -0.0753106053470809 1107 3 -0.09114278677535737 1107 4 0.024202765931711667 1107 5 0.007947793663873881 1107 6 -0.08077758695204551 1107 7 0.10128844721549513 1107 8 -0.06177550979389679 1107 9 0.0684353673592355 1107 10-11 -0.0632067283365032 1107 12-13 0.15417895818229255 1107 14-15 -0.03478385983873977 1107 16-17 -0.03455261076415894 1107 18-19 0.08435684537832344 1107 20-21 0.21543138313520416 1107 22-23 0.21470993240003367 1107 24-25 0.07997990519665876 1107 26-27 0.13930857043104083 1107 28-29 0.09943699725147326 1107 30-31 0.1516699540398605 1107 32-33 0.18928571262923555 1107 34-35 -0.001495424235351095 1107 36-37 -0.16582542273690848 1107 38-39 -0.05153600848640849 1107 40-41 0.10194743904463621 1107 42-43 0.09556937891719741 1107 44-45 0.10175033115477561 1107 46-47 0.15102286080698946 1107 48-49 0.08249301807233422 1107 50-51 -0.045780924809385226 1107 52-53 -0.026085068167255088 1107 54-55 0.10931388324897284 1107 56-57 -0.08961861828783668 1107 58-59 0.0097074596311586 1107 60-61 0.38308698232658145 1107 62-63 0.4909792464392311 1107 64-65 0.5090427838122764 1107 66-67 0.2418279305176938 1107 68-69 0.11443647674166613 1107 70-71 0.39959327128409683 1107 72-73 0.5273879043564378 1107 74-75 0.5741519810496918 1107 76-77 0.4810038200685973 1107 78-79 0.45454690152428867 1107 80-81 0.6082144296708734 1107 82-83 0.6239490445481728 1107 84-85 0.5561220783726277 1107 86-87 0.6414058627832624 1107 88-89 0.6339282107333055 1107 90-91 0.7584616299411735 1107 92-93 0.8585615849134243 1107 94-95 0.9807352879934896 1107 96-97 0.9156831263747272 1107 98-99 0.8536398576788251 1107 100-101 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0.4057429666313368 2316 16-17 0.07893888849134356 2316 18-19 0.23358595051172415 2316 20-21 0.17684010845070475 2316 22-23 0.1433506676259455 2316 24-25 0.3025828539114812 2316 26-27 0.38877689249514447 2316 28-29 0.3341708989284342 2316 30-31 0.1351002149815912 2316 32-33 0.04110470718583059 2316 34-35 0.07496169368883443 2316 36-37 -0.02063005207272539 2316 38-39 -0.2645145949096275 2316 40-41 -0.06883331131540871 2316 42-43 0.0075023066395090154 2316 44-45 0.04058143301725181 2316 46-47 0.14256864765982158 2316 48-49 0.2753288676585939 2316 50-51 0.052281234977755275 2316 52-53 0.06129318750193846 2316 54-55 0.028595713746689455 2316 56-57 0.2797770233261474 2316 58-59 0.5556003429274696 2316 60-61 0.4905477129146192 2316 62-63 0.5197302838595945 2316 64-65 0.5595567645772874 2316 66-67 0.3735887074845152 2316 68-69 0.4906722274678543 2316 70-71 0.3527415369613536 2316 72-73 0.42974452675984054 2316 74-75 0.7240683771734062 2316 76-77 0.6912607036578251 2316 78-79 0.7229855228536515 2316 80-81 0.8277325784247473 2316 82-83 0.45988864227317094 2316 84-85 0.6469078022479415 2316 86-87 0.6182233317216763 2316 88-89 0.5665797961557146 2316 90-91 0.2685833437596159 2316 92-93 0.4045582102460088 2316 94-95 0.9141786692515765 2316 96-97 1.0600355929942005 2316 98-99 1.233710249701673 2316 100-101 0.9175248186498237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4186.0 3 956.0 4 696.0 5 707.0 6 763.0 7 971.0 8 1188.0 9 1419.0 10 1849.0 11 2379.0 12 3272.0 13 4304.0 14 4914.0 15 6217.0 16 7901.0 17 9834.0 18 8930.0 19 6424.0 20 6106.0 21 6340.0 22 6865.0 23 7571.0 24 8552.0 25 9849.0 26 11213.0 27 13197.0 28 15820.0 29 18706.0 30 23208.0 31 29179.0 32 36742.0 33 48308.0 34 65788.0 35 93041.0 36 137887.0 37 197605.0 38 163701.0 39 20329.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.458977637275204 19.381671319432243 13.920722561461696 22.238628481830858 2 14.761704607524816 20.56502131063989 41.863274833139414 22.809999248695878 3 19.76697085460602 25.354939509272334 29.561321669355255 25.316767966766395 4 12.195166523852649 16.822239188437344 37.85884560247887 33.123748685231135 5 13.011175061753713 38.24334161454108 34.25272675770919 14.492756565996014 6 30.59489826072916 36.69072419350417 18.113215594639303 14.601161951127374 7 28.03013223983118 33.32500614384103 20.82579316479914 17.81906845152865 8 25.582849994313662 35.923766470081716 19.96454159964907 18.528841935955548 9 26.0807892297273 17.402795730150203 21.106676184103513 35.40973885601898 10-11 24.695624888615388 27.434505848358516 27.082847810667232 20.787021452358868 12-13 25.65878897275176 24.893715869884765 27.96187835906341 21.485616798300065 14-15 22.369221303111313 26.22249816984452 26.818149050299066 24.590131476745096 16-17 21.29565974143875 29.75739192511452 27.343192279922864 21.603756053523863 18-19 21.11500002030762 28.101481237790043 30.038828169105614 20.744690572796724 20-21 22.028981681351777 27.067523743575478 30.08560277307904 20.81789180199371 22-23 21.632721647799297 27.0870234231545 30.027825117537134 21.252429811509067 24-25 21.444580096095518 27.212747758152002 30.224095963531422 21.118576182221062 26-27 21.368978419019285 27.370328816777423 30.32470466394895 20.93598810025434 28-29 20.872719703085412 27.743997481709393 30.310623023065713 21.072659792139483 30-31 21.26675237056579 27.360157435219428 30.208796631958617 21.164293562256166 32-33 20.99863728952622 27.601792035353302 30.12696968527684 21.272600989843642 34-35 21.477903498028894 27.524509355902048 29.966305347883576 21.031281798185486 36-37 21.397930796409863 27.431771108313363 30.039241765828695 21.13105632944808 38-39 21.15846384242455 27.74226463943955 30.147523923141357 20.951747594994544 40-41 21.414241404342373 27.673169567843892 29.92253374553909 20.990055282274646 42-43 21.158591840309064 27.72553628404842 29.646944748338033 21.46892712730448 44-45 21.284466646932877 27.838860571633404 29.459231529654755 21.41744125177896 46-47 21.48079055654173 28.065072040490534 29.08119432450108 21.37294307846666 48-49 21.442321118922308 28.094465559219962 29.02454814803531 21.438665173822418 50-51 21.356679049381235 28.300223620044967 29.042932611358115 21.300164719215683 52-53 21.529910341342774 28.67208190398322 28.502972117198667 21.295035637475337 54-55 21.310016217923394 28.334042507817 28.72842137759936 21.627519896660242 56-57 21.467794804993062 28.537865515853213 28.55954226026003 21.434797418893694 58-59 21.75745639859501 28.21112419548048 28.58343654193653 21.44798286398798 60-61 21.47882373422813 28.382180295309972 28.99541721885229 21.143578751609606 62-63 21.356391105678693 28.46229517183688 28.996541232628754 21.184772489855668 64-65 21.291669437142513 28.449953024572892 28.84098348424179 21.417394054042806 66-67 21.168310017145796 28.466999160475126 28.63297535938669 21.731715462992383 68-69 21.091835849009676 28.432836086051914 28.788094896079564 21.687233168858842 70-71 21.287466683450777 28.490592183103846 28.65281605102641 21.569125082418964 72-73 21.32170316575882 28.19625298660084 28.74461498165782 21.737428865982523 74-75 21.03266880983237 28.73400430359044 28.529535534062983 21.70379135251421 76-77 21.18845025076035 28.432502846722702 28.577237691573142 21.8018092109438 78-79 21.219020525480317 28.44829643031373 28.429412155888674 21.903270888317284 80-81 21.242941524041967 28.685433092450047 28.379895754863522 21.691729628644467 82-83 21.303691701855826 28.589484520069625 28.442382924032533 21.66444085404202 84-85 21.18552441361835 28.399815639009812 28.353420830355404 22.061239117016438 86-87 21.220618715794913 28.369643624940927 28.470206135379172 21.93953152388499 88-89 21.27355109615771 28.162227686484886 28.313448109922373 22.25077310743503 90-91 21.294977322870142 27.992472431575177 28.588971817815377 22.1235784277393 92-93 21.428575054378037 27.99495432752709 28.492567277748027 22.083903340346854 94-95 21.15472763515959 27.959622806434243 28.630173353306027 22.255476205100145 96-97 20.960914830149676 27.956008877824324 28.752921539283154 22.330154752742846 98-99 21.03098896435066 28.16887823015355 28.386616292635082 22.41351651286071 100-101 21.040444589496275 28.024436383127 28.606871362319225 22.328247665057503 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1430.0 1 861.5 2 337.5 3 382.5 4 341.5 5 333.0 6 509.5 7 912.0 8 1428.0 9 1712.5 10 1570.0 11 1422.5 12 1473.0 13 1572.5 14 1672.0 15 1771.5 16 1848.5 17 1887.0 18 2024.5 19 2245.0 20 2487.5 21 2782.0 22 3163.5 23 3826.5 24 4576.5 25 5490.5 26 6908.5 27 8670.5 28 10175.0 29 11858.0 30 13868.5 31 15470.5 32 17305.0 33 19385.5 34 21858.0 35 24162.5 36 26729.0 37 29568.0 38 31132.5 39 31761.5 40 32076.0 41 32979.0 42 34239.5 43 35446.0 44 36606.0 45 37366.5 46 37075.5 47 38044.0 48 42189.0 49 41515.0 50 36358.5 51 33015.0 52 30480.5 53 28786.0 54 27100.5 55 24392.5 56 21293.5 57 18643.0 58 16626.0 59 14672.5 60 12314.5 61 9898.0 62 7618.5 63 5712.0 64 4244.5 65 3169.0 66 2307.0 67 1688.0 68 1273.5 69 910.0 70 686.5 71 525.5 72 383.5 73 290.0 74 227.0 75 182.5 76 136.5 77 75.0 78 43.0 79 32.5 80 26.5 81 21.0 82 13.5 83 7.5 84 5.5 85 5.5 86 5.0 87 4.5 88 4.0 89 2.5 90 2.0 91 1.5 92 1.5 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22332036035232739 2 0.19950897891730154 3 0.19211225191833609 4 0.19849572864347065 5 0.19879970372561995 6 0.22291506024279506 7 0.22261108516064576 8 0.21349183269616778 9 0.20913485651869496 10-11 0.21881139663377994 12-13 0.2209392222088248 14-15 0.20609510569720232 16-17 0.21947000931177002 18-19 0.20984413171037658 20-21 0.20518318045075454 22-23 0.2048285428549137 24-25 0.19915434132146076 26-27 0.20548715553290378 28-29 0.21561965827121263 30-31 0.21653158351766047 32-33 0.21491038307953103 34-35 0.22261108516064576 36-37 0.20295402984832656 38-39 0.20244740471141112 40-41 0.20042090416374936 42-43 0.20472721782753062 44-45 0.21769682133256596 46-47 0.22281373521541195 48-49 0.22549884844106377 50-51 0.22463758570830752 52-53 0.23867110200086533 54-55 0.22392831051662593 56-57 0.2022954171703365 58-59 0.18876852601469415 60-61 0.18466486240567906 62-63 0.19150430175403757 64-65 0.18577943770689304 66-67 0.1866407004396493 68-69 0.18912316361053497 70-71 0.18892051355576878 72-73 0.1923655644867938 74-75 0.19570929039043572 76-77 0.20452456777276443 78-79 0.2002689166226747 80-81 0.1962665780410427 82-83 0.191453639240346 84-85 0.19241622700048533 86-87 0.19895169126669457 88-89 0.1962665780410427 90-91 0.2036633050400082 92-93 0.19459471508922174 94-95 0.19368278984277396 96-97 0.20234607968402804 98-99 0.20508185542337143 100-101 0.18588076273427614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 986923.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.12646484407378 #Duplication Level Percentage of deduplicated Percentage of total 1 84.63607763601142 56.8132068997402 2 9.459799391999182 12.700057826380473 3 2.4779796983140585 4.990140513096216 4 1.0513798831183554 2.8230165904764277 5 0.5536442648370303 1.8582091139852999 6 0.37076584264735407 1.4932920181110603 7 0.2549806911835666 1.1981166681855924 8 0.17917306897900923 0.9621803772659417 9 0.14028288816206658 0.8475024924392487 >10 0.8097415860148949 9.947192074310184 >50 0.04568070837354309 2.0746606225805935 >100 0.018535323137757766 2.182223918896517 >500 0.0012055490595933112 0.5966140078122336 >1k 7.534681622458194E-4 1.5135868767199814 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4950 0.5015588855462888 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4048 0.41016371084674286 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2576 0.26101327053883633 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2068 0.20954015662822734 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1321 0.1338503611730601 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 993 0.10061575219140703 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.0132502738308865E-4 0.0 24-25 1.0132502738308865E-4 0.0 0.0 3.0397508214926596E-4 0.0 26-27 1.0132502738308865E-4 0.0 0.0 4.053001095323546E-4 0.0 28-29 1.0132502738308865E-4 0.0 0.0 0.0015198754107463297 0.0 30-31 1.0132502738308865E-4 0.0 0.0 0.003597038472099647 0.0 32-33 1.0132502738308865E-4 0.0 0.0 0.006890101862050028 0.0 34-35 1.0132502738308865E-4 0.0 0.0 0.011044427984756662 0.0 36-37 1.0132502738308865E-4 0.0 0.0 0.015654716730687197 0.0 38-39 2.026500547661773E-4 0.0 0.0 0.021734218373672516 0.0 40-41 2.026500547661773E-4 0.0 0.0 0.028421670180956368 0.0 42-43 2.026500547661773E-4 0.0 0.0 0.043569761774728114 0.0 44-45 2.026500547661773E-4 0.0 0.0 0.06028839129293775 0.0 46-47 2.026500547661773E-4 0.0 0.0 0.07700702081114738 0.0 48-49 2.026500547661773E-4 0.0 0.0 0.10238894017061108 0.0 50-51 2.026500547661773E-4 0.0 0.0 0.13390102368675166 0.0 52-53 2.026500547661773E-4 0.0 0.0 0.18537413759736068 0.0 54-55 2.026500547661773E-4 0.0 0.0 0.2244855981672329 0.0 56-57 2.026500547661773E-4 0.0 0.0 0.305190982477863 0.0 58-59 2.026500547661773E-4 0.0 0.0 0.4113289486616484 0.0 60-61 2.026500547661773E-4 0.0 0.0 0.5212666033722996 0.0 62-63 2.026500547661773E-4 0.0 0.0 0.6884528985543958 0.0 64-65 2.026500547661773E-4 0.0 0.0 0.8077124557842912 0.0 66-67 2.026500547661773E-4 0.0 0.0 1.0125916611528964 0.0 68-69 2.026500547661773E-4 0.0 0.0 1.2590647902622596 0.0 70-71 2.026500547661773E-4 0.0 0.0 1.4639439956308649 0.0 72-73 2.026500547661773E-4 0.0 0.0 1.7525176736179013 0.0 74-75 2.026500547661773E-4 0.0 0.0 2.053047704836142 0.0 76-77 2.026500547661773E-4 0.0 0.0 2.2744935521818825 0.0 78-79 2.026500547661773E-4 0.0 0.0 2.512708691559524 0.0 80-81 2.026500547661773E-4 0.0 0.0 2.9107133991202963 0.0 82-83 2.026500547661773E-4 0.0 0.0 3.2485817029292052 0.0 84-85 2.026500547661773E-4 0.0 0.0 3.6742481429655607 1.0132502738308865E-4 86-87 2.026500547661773E-4 0.0 0.0 4.243441484290061 1.0132502738308865E-4 88-89 2.026500547661773E-4 0.0 0.0 4.967358142428538 1.0132502738308865E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 845 0.0 30.921722 2 GTACATG 4950 0.0 26.206161 1 GTACCTG 1065 0.0 25.431454 1 TACATGG 4860 0.0 25.12202 2 ACATGGG 4885 0.0 23.63194 3 GTATCAA 8105 0.0 23.391918 1 GTATAGG 425 0.0 22.360785 1 ACCTGGG 1160 0.0 21.70579 3 GAGTACT 3830 0.0 21.64154 12-13 CATGGGG 1655 0.0 21.529951 4 AGTACTT 4020 0.0 20.914078 12-13 CAACGCA 9020 0.0 20.753508 5 ATCAACG 9025 0.0 20.739902 3 TCAACGC 9070 0.0 20.63805 4 TATCAAC 9175 0.0 20.556166 2 AACGCAG 9190 0.0 20.46988 6 GTACTTT 4145 0.0 19.938578 14-15 TAGACTG 460 0.0 19.624454 5 GGTATCA 5955 0.0 18.910915 1 CATGGGA 3370 0.0 18.890987 4 >>END_MODULE