FastQCFastQC Report
Thu 26 May 2016
SRR937256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986923
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66550.674318057234455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58760.5953858609030289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39750.40276698384777737No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19010.19261887705525152No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14450.14641466456856309No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11320.11469993099765637No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9970.1010210523009394No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1107.014387E-834.543562
TAACGCA1151.03664206E-733.0416644
ATAACGC1252.1544292E-730.3983293
GTATACG804.4946308E-429.6858714
GGTATCA59150.029.2439791
GTATCAA81050.025.2118821
GAACCGT1151.2820517E-424.7812486
GCGGTAT1351.3377852E-524.640761
GGACCGA1951.5701517E-824.3576356
GTACATG49050.023.6396271
TACCTGG8900.022.948182
GAGTACT55500.022.59793312-13
TACATGG49200.022.5902212
GTATAGG3200.022.2756881
ACATGGG49050.022.0782973
GTACTTT56950.021.64279714-15
ATCAACG94700.021.3161473
GTACCTG10050.021.2782671
TATCAAC96050.021.0659962
TCAACGC95850.021.0603984
Produced by FastQC (version 0.11.5)