##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937256_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 986923 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.271265336809456 33.0 31.0 34.0 31.0 34.0 2 32.46662606910569 34.0 31.0 34.0 31.0 34.0 3 32.523843298818655 34.0 31.0 34.0 31.0 34.0 4 36.14920008957132 37.0 35.0 37.0 35.0 37.0 5 36.08412611723508 37.0 35.0 37.0 35.0 37.0 6 36.12897358760511 37.0 35.0 37.0 35.0 37.0 7 36.07597046578102 37.0 35.0 37.0 35.0 37.0 8 36.05436493019212 37.0 35.0 37.0 35.0 37.0 9 37.77765235991055 39.0 37.0 39.0 35.0 39.0 10-11 37.703818332331906 39.0 37.0 39.0 35.0 39.0 12-13 37.60974159078266 39.0 37.0 39.0 35.0 39.0 14-15 38.82729757032717 40.0 38.0 41.0 35.0 41.0 16-17 38.79751915802955 40.0 38.0 41.0 35.0 41.0 18-19 38.81274780302009 40.0 38.0 41.0 35.0 41.0 20-21 38.73127032200081 40.0 38.0 41.0 34.5 41.0 22-23 38.59056076309905 40.0 38.0 41.0 34.0 41.0 24-25 38.5287722547757 40.0 38.0 41.0 34.0 41.0 26-27 38.47719072308579 40.0 38.0 41.0 34.0 41.0 28-29 38.35611592799033 40.0 38.0 41.0 34.0 41.0 30-31 38.40749075662438 40.0 38.0 41.0 34.0 41.0 32-33 38.318667717744944 40.0 38.0 41.0 34.0 41.0 34-35 38.184324916938806 40.0 38.0 41.0 34.0 41.0 36-37 38.01016897974817 40.0 37.0 41.0 33.5 41.0 38-39 37.87579831455949 40.0 37.0 41.0 33.0 41.0 40-41 37.69391685065603 40.0 37.0 41.0 33.0 41.0 42-43 37.4629069339756 40.0 37.0 41.0 33.0 41.0 44-45 37.16410500109937 40.0 37.0 41.0 32.0 41.0 46-47 36.80422332846635 40.0 36.5 41.0 31.0 41.0 48-49 36.45171406482572 40.0 36.0 41.0 30.0 41.0 50-51 36.19616373313825 39.0 36.0 41.0 30.0 41.0 52-53 35.944022481997074 39.0 35.5 41.0 29.0 41.0 54-55 35.711350328242425 39.0 35.0 40.5 28.0 41.0 56-57 35.46269516466837 39.0 35.0 40.0 28.0 41.0 58-59 35.20479155921991 38.5 35.0 40.0 27.5 41.0 60-61 34.88634776978549 38.0 34.5 40.0 26.5 41.0 62-63 34.539436713907776 38.0 34.0 40.0 26.0 41.0 64-65 34.15988633358428 37.0 34.0 40.0 26.0 41.0 66-67 33.75987539048133 37.0 33.5 39.5 25.0 41.0 68-69 33.34046678413615 36.0 33.0 39.0 23.5 40.0 70-71 32.94527485933553 36.0 33.0 39.0 22.5 40.0 72-73 32.48214551692483 35.0 32.0 38.0 21.5 40.0 74-75 32.00678067083248 35.0 32.0 37.0 20.0 39.0 76-77 30.725544444703388 34.0 30.5 36.0 19.5 38.5 78-79 31.321105091278653 35.0 31.0 36.0 20.0 39.0 80-81 31.11700102236953 35.0 31.0 36.0 18.5 38.0 82-83 30.76467211727764 34.0 31.0 35.5 17.5 37.0 84-85 30.40700085011698 34.0 31.0 35.0 13.0 37.0 86-87 30.00536262707425 34.0 30.5 35.0 7.0 36.0 88-89 29.557378336506495 34.0 30.0 35.0 3.5 36.0 90-91 29.071697082751136 34.0 29.0 35.0 2.0 35.5 92-93 28.739666620394907 34.0 29.0 35.0 2.0 35.0 94-95 28.339283307816313 34.0 29.0 35.0 2.0 35.0 96-97 27.8945246994953 34.0 29.0 35.0 2.0 35.0 98-99 27.205199899080274 33.0 27.0 35.0 2.0 35.0 100-101 24.762898929298437 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04713459727110347 1101 2 -0.07127431346335555 1101 3 -0.032237872466687634 1101 4 0.006718171043694099 1101 5 -0.027015906359054043 1101 6 -0.10479710275949827 1101 7 -0.018861110925826097 1101 8 -0.024638504303489128 1101 9 0.016241982057195514 1101 10-11 -0.023625437386442627 1101 12-13 -0.007530340045640571 1101 14-15 -0.13178959325362882 1101 16-17 -0.15264441443894583 1101 18-19 -0.0747488733064472 1101 20-21 -0.06333343032979144 1101 22-23 0.04682249851369846 1101 24-25 0.02956027038971598 1101 26-27 -0.13545865239337473 1101 28-29 -0.11060992015599425 1101 30-31 -0.10769196869303954 1101 32-33 0.03153123321965978 1101 34-35 -0.09457123826933866 1101 36-37 0.06740645240482479 1101 38-39 -0.11737549159375504 1101 40-41 -0.08896178941744637 1101 42-43 -0.11600749190370152 1101 44-45 -0.11614629869467308 1101 46-47 -0.09347103769606946 1101 48-49 -0.017497666776932874 1101 50-51 -0.09721180936438145 1101 52-53 -0.024541141523101828 1101 54-55 0.0741738373091465 1101 56-57 -0.005706673315572175 1101 58-59 -0.014647698177064683 1101 60-61 -0.01639318526663658 1101 62-63 -0.16913524313920192 1101 64-65 -0.0652999084692496 1101 66-67 -0.16177556349612843 1101 68-69 -0.22236604263001425 1101 70-71 -0.1999021893070818 1101 72-73 -0.0022412658711417066 1101 74-75 -0.013961842153712212 1101 76-77 0.11535479465062437 1101 78-79 -0.00364205088267866 1101 80-81 -0.07293213490000383 1101 82-83 -0.04237238016426659 1101 84-85 -0.15783803376883654 1101 86-87 -0.13066083167564457 1101 88-89 -0.1600682276841887 1101 90-91 -0.2211565318922588 1101 92-93 -0.1212936707132819 1101 94-95 -0.03731844388637384 1101 96-97 -0.13062737251173573 1101 98-99 -0.09493225180301934 1101 100-101 0.5920367784225782 1102 1 -0.10922820364339003 1102 2 -0.026859050522979544 1102 3 -0.042843140669511115 1102 4 -0.060763876085879076 1102 5 -0.06697845555532211 1102 6 -0.09253869873071352 1102 7 -0.10396827963746347 1102 8 -0.11091166599092617 1102 9 -0.10624810537326823 1102 10-11 -0.12563597422655448 1102 12-13 -0.1785611984000326 1102 14-15 -0.0667750305607342 1102 16-17 -0.16886350164077157 1102 18-19 -0.09446838329613172 1102 20-21 -0.05931436604593898 1102 22-23 0.04877025529418688 1102 24-25 -0.13599498285125122 1102 26-27 -0.14618665669891584 1102 28-29 -0.3324426634327935 1102 30-31 -0.11430043889613017 1102 32-33 0.04036796107516949 1102 34-35 -0.1538940499688053 1102 36-37 -0.1455260226447166 1102 38-39 0.02208421973744379 1102 40-41 0.022829076952810112 1102 42-43 -0.023914412319115286 1102 44-45 -0.1199942927468669 1102 46-47 0.09731164391399005 1102 48-49 0.40743780173012567 1102 50-51 0.18766112889990438 1102 52-53 0.3866457111593604 1102 54-55 0.454901499474218 1102 56-57 0.3325483234286679 1102 58-59 0.3296523231659947 1102 60-61 0.2370484491003424 1102 62-63 0.28899789729207725 1102 64-65 0.2684602858230889 1102 66-67 0.29863824181725107 1102 68-69 0.18382963238868655 1102 70-71 0.14411325806329955 1102 72-73 0.12097736351416444 1102 74-75 0.15581368654834904 1102 76-77 0.21991101946188962 1102 78-79 0.3084739575437503 1102 80-81 0.16895465570840074 1102 82-83 0.1963395641765402 1102 84-85 -0.12094958948152268 1102 86-87 0.005937390605954107 1102 88-89 0.17498178184686353 1102 90-91 0.0178807174287563 1102 92-93 0.05399656486943272 1102 94-95 0.1207349723861526 1102 96-97 0.032468814034107396 1102 98-99 0.11984751020841955 1102 100-101 0.8892463242501201 1103 1 0.007173903616774169 1103 2 -0.012681062257243525 1103 3 -0.009051414320751405 1103 4 -0.049064170450336064 1103 5 -0.032784145065612336 1103 6 -0.10712675839610597 1103 7 -0.10861556351918011 1103 8 -0.1851651143055122 1103 9 -0.1804819716266408 1103 10-11 -0.11904200308244839 1103 12-13 0.0031551462281171894 1103 14-15 -0.15941488247427316 1103 16-17 -0.2579227160660764 1103 18-19 -0.14243118659490506 1103 20-21 -0.15893983032796655 1103 22-23 -0.08918824571054529 1103 24-25 -0.07921506851700855 1103 26-27 -0.11494462990950893 1103 28-29 -0.19967427256563752 1103 30-31 -0.208751919443948 1103 32-33 -0.1287697889344841 1103 34-35 -0.11966086332814996 1103 36-37 -0.18744084874161615 1103 38-39 -0.17880008310950046 1103 40-41 -0.23747151920576215 1103 42-43 -0.17423668121191582 1103 44-45 -0.18625077282528224 1103 46-47 0.05314545671985371 1103 48-49 -0.2314902992764445 1103 50-51 -0.09475020611863272 1103 52-53 0.008149233338635042 1103 54-55 -0.15869922114563195 1103 56-57 -0.28142042475351303 1103 58-59 -0.30117888349790434 1103 60-61 -0.28345684871112553 1103 62-63 -0.20008107197732983 1103 64-65 -0.3045998086430757 1103 66-67 -0.11876404801683549 1103 68-69 -0.2706455309343454 1103 70-71 -0.1436831437905468 1103 72-73 -0.1823386857704321 1103 74-75 -0.07045386315409274 1103 76-77 0.14337280535739083 1103 78-79 -0.09635808884500463 1103 80-81 -0.271067218605868 1103 82-83 -0.22109584237872681 1103 84-85 -0.227554042787812 1103 86-87 -0.11507398829944293 1103 88-89 -0.023281404511582338 1103 90-91 -0.0702376941435503 1103 92-93 -0.1744339386832685 1103 94-95 -0.3098149437277513 1103 96-97 -0.1473898265216107 1103 98-99 -0.011028044064701703 1103 100-101 0.17759217570405994 1104 1 0.04708353236028273 1104 2 0.12819742215874896 1104 3 0.04385900206214188 1104 4 0.01957297651843959 1104 5 -0.09393516455524775 1104 6 -0.018950232418809776 1104 7 -0.02200780201401642 1104 8 0.07555359183393051 1104 9 0.1350333399646857 1104 10-11 0.06014299742093243 1104 12-13 0.06273384798520709 1104 14-15 0.07211319716613218 1104 16-17 0.08627599410039721 1104 18-19 0.08752042984247765 1104 20-21 0.018463506183557854 1104 22-23 -0.057832203095109946 1104 24-25 0.06100428609245512 1104 26-27 -0.12857641737715397 1104 28-29 -0.07112885893651111 1104 30-31 -0.06242066081988895 1104 32-33 -0.014958265710966145 1104 34-35 -0.04956154969961801 1104 36-37 0.06031704393808468 1104 38-39 -0.017620637536559514 1104 40-41 -0.1222751127364603 1104 42-43 -0.13317105841419163 1104 44-45 -0.00412130386005316 1104 46-47 0.08269703525579075 1104 48-49 0.09840387278426732 1104 50-51 0.13396221074987835 1104 52-53 0.19519226924433752 1104 54-55 0.3449520500332852 1104 56-57 0.1630101356548721 1104 58-59 0.11754739108621237 1104 60-61 0.2515264805754356 1104 62-63 0.239719886218559 1104 64-65 0.257096044109808 1104 66-67 0.19977449597548969 1104 68-69 0.13638577921199158 1104 70-71 0.1587108654123952 1104 72-73 0.3926863351049761 1104 74-75 0.3306639333506851 1104 76-77 0.5816991604820814 1104 78-79 0.30874172816208656 1104 80-81 0.24667954052453211 1104 82-83 0.351752214283934 1104 84-85 0.49112219004550184 1104 86-87 0.36906721826935396 1104 88-89 0.4853823326892801 1104 90-91 0.527012970612251 1104 92-93 0.8616494208060068 1104 94-95 0.9798068709223422 1104 96-97 0.8352261430151025 1104 98-99 1.0406049751998907 1104 100-101 1.5217295921284126 1105 1 -0.06072769237445641 1105 2 -0.07899447375559987 1105 3 -0.16105482001284344 1105 4 -0.015504319559148882 1105 5 -0.04072689723564338 1105 6 -0.100526400261721 1105 7 -0.11676751203370372 1105 8 -4.618193716012797E-4 1105 9 -0.016755032016682492 1105 10-11 -0.06092018372074648 1105 12-13 -0.09355486006607094 1105 14-15 -0.13386944722389416 1105 16-17 -0.18332348259075104 1105 18-19 -0.1384637748721076 1105 20-21 -0.056580302928736614 1105 22-23 -0.15220483260204531 1105 24-25 -0.23302927288781206 1105 26-27 -0.09429548694362211 1105 28-29 -0.16679415731858427 1105 30-31 -0.1172498652944185 1105 32-33 0.04620465617679059 1105 34-35 -0.014431442637345526 1105 36-37 -0.011717139172006341 1105 38-39 -0.055034219561626685 1105 40-41 -0.14299802310181775 1105 42-43 -0.07287891764892152 1105 44-45 -0.10140213827361322 1105 46-47 -0.2838796126869809 1105 48-49 -0.15922851475234268 1105 50-51 -0.21823124548902229 1105 52-53 -0.1866863615108656 1105 54-55 -0.24943798309360687 1105 56-57 -0.20156749649261485 1105 58-59 -0.41260948154329213 1105 60-61 -0.39771556176937395 1105 62-63 -0.15183935810426163 1105 64-65 -0.13941062927825243 1105 66-67 0.0675621958170538 1105 68-69 -0.11234915765487585 1105 70-71 0.020201455455129746 1105 72-73 0.14340228402235056 1105 74-75 -0.045199046149051014 1105 76-77 0.09754965738543575 1105 78-79 -0.03912062720782927 1105 80-81 0.012879310076598927 1105 82-83 0.0314001810610236 1105 84-85 0.2241560610906781 1105 86-87 -0.11239072498284486 1105 88-89 0.1188104857451151 1105 90-91 0.19643000935630894 1105 92-93 0.25797185587250127 1105 94-95 0.3761173034923182 1105 96-97 0.3015897466446553 1105 98-99 0.42204071383352826 1105 100-101 0.9265461939815971 1106 1 -0.21959756204962133 1106 2 -0.1258725130475682 1106 3 -0.04460536141205296 1106 4 -0.0658917279751492 1106 5 -0.13880282280135958 1106 6 -0.14070989244871868 1106 7 -0.17709948376423768 1106 8 -0.12084209558245362 1106 9 -0.052950973143573776 1106 10-11 -0.23500272278577938 1106 12-13 -0.13593879963683975 1106 14-15 -0.17529702292252836 1106 16-17 -0.08133700136141897 1106 18-19 0.03832576023779666 1106 20-21 -0.06485933424559676 1106 22-23 -0.1905444876626703 1106 24-25 -0.21677108706145276 1106 26-27 -0.3329447445459053 1106 28-29 -0.34362826871065266 1106 30-31 -0.31089328745611766 1106 32-33 -0.38329592736726426 1106 34-35 -0.2283894923034282 1106 36-37 -0.5918490849672509 1106 38-39 -0.6015678057640557 1106 40-41 -0.4839701102443925 1106 42-43 -0.7976001132658581 1106 44-45 -0.6514856174929591 1106 46-47 -0.8868427197348936 1106 48-49 -0.8725543916893841 1106 50-51 -0.8179402839380856 1106 52-53 -0.6474430236388571 1106 54-55 -0.8354458933575017 1106 56-57 -0.9840879205346269 1106 58-59 -0.9503331734320213 1106 60-61 -0.9304467100543974 1106 62-63 -0.7333369590898045 1106 64-65 -0.6309046641612213 1106 66-67 -0.5623979286723397 1106 68-69 -0.4689918341769541 1106 70-71 -0.6305109545818297 1106 72-73 -0.778163721294387 1106 74-75 -0.6888076177628761 1106 76-77 -0.693946808931102 1106 78-79 -0.6370263524234154 1106 80-81 -0.7447634961219372 1106 82-83 -0.8552747524797937 1106 84-85 -0.7344568871035762 1106 86-87 -0.7725614116196873 1106 88-89 -0.5470781221781174 1106 90-91 -0.518253417992451 1106 92-93 -0.31429034992403615 1106 94-95 -0.2501713563014967 1106 96-97 -0.19316240922644212 1106 98-99 -0.186036508496759 1106 100-101 0.11870238927434329 1107 1 -0.14981330668928194 1107 2 -0.18623382179351466 1107 3 -0.04966319985162926 1107 4 -0.040971588582088714 1107 5 -0.04856638700444904 1107 6 -0.20786540283059196 1107 7 -0.06576653763384144 1107 8 -0.0826269801475803 1107 9 -0.1282407703460322 1107 10-11 -0.19089183754305594 1107 12-13 -0.19062667776471898 1107 14-15 -0.1070872920285737 1107 16-17 -0.04004241252254559 1107 18-19 -0.07604128395173859 1107 20-21 -0.18243290280472024 1107 22-23 -0.1414892023425054 1107 24-25 -0.16712440974669818 1107 26-27 -0.13308601311954504 1107 28-29 -0.056706350604081024 1107 30-31 -0.16692488079623047 1107 32-33 -0.06221393714414347 1107 34-35 0.011647594478432666 1107 36-37 -0.028387127656372968 1107 38-39 -0.03366662721509073 1107 40-41 0.07461702251781333 1107 42-43 -0.03661020474255139 1107 44-45 -0.07590995561793079 1107 46-47 0.061276623472110714 1107 48-49 0.16177810673760007 1107 50-51 0.20180706614176813 1107 52-53 0.12964756974965042 1107 54-55 0.4172010017588974 1107 56-57 0.33755521145317857 1107 58-59 0.2048444559179572 1107 60-61 0.08623175420193263 1107 62-63 0.27550972064496193 1107 64-65 0.10832776650525489 1107 66-67 0.24836873315428676 1107 68-69 0.20178130119887783 1107 70-71 0.20944264156550219 1107 72-73 0.3591072844652601 1107 74-75 0.27031827159388655 1107 76-77 0.32947896910481234 1107 78-79 0.12877788018875336 1107 80-81 0.3034220950885569 1107 82-83 0.34983239383451803 1107 84-85 0.44469707446653217 1107 86-87 0.6276355173769765 1107 88-89 0.43725377124427567 1107 90-91 0.6637516277458921 1107 92-93 0.8116413571569403 1107 94-95 0.4943684772012382 1107 96-97 0.6337881255976221 1107 98-99 0.6296155654590017 1107 100-101 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74-75 -0.002416490531736315 2316 76-77 -0.31736483200339904 2316 78-79 -0.3028614575709092 2316 80-81 -0.3936435309030948 2316 82-83 -0.39327867727149624 2316 84-85 -0.5114688639717251 2316 86-87 -0.7997885373500004 2316 88-89 -0.5814922621484548 2316 90-91 -0.5801939565048464 2316 92-93 -0.31136313426298656 2316 94-95 -0.06813457403512402 2316 96-97 0.18181153468298206 2316 98-99 -0.11065251616767213 2316 100-101 -0.6597315056794102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 8.0 5 19.0 6 29.0 7 88.0 8 223.0 9 403.0 10 812.0 11 1312.0 12 2116.0 13 3130.0 14 4080.0 15 5595.0 16 7800.0 17 10754.0 18 10320.0 19 6444.0 20 5062.0 21 4691.0 22 4867.0 23 5250.0 24 5758.0 25 6926.0 26 8291.0 27 10221.0 28 12847.0 29 15872.0 30 20321.0 31 25282.0 32 32867.0 33 43422.0 34 60421.0 35 88474.0 36 137889.0 37 222618.0 38 201638.0 39 21070.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63304538264996 19.731921969420448 14.73212475159184 21.90290789633775 2 15.176259951384253 20.70820114639136 41.201795884785334 22.91374301743905 3 19.920703033569996 25.144109520195595 30.244102123468597 24.691085322765808 4 12.54221453953348 17.209346625825926 37.70891954083551 32.53951929380509 5 12.966469991600018 37.873274786783355 34.477818072016916 14.682437149599709 6 30.76552071438197 36.75109405698317 17.72843474110949 14.75495048752537 7 27.736003720655006 33.61366590909321 20.824420952799763 17.82590941745202 8 25.769487589204022 35.6244610775106 19.88736709956096 18.718684233724414 9 25.96828729292964 17.128185278892072 21.163251844368812 35.74027558380948 10-11 24.788927195397815 27.15116941806474 27.112159262758727 20.947744123778715 12-13 25.7352408446701 24.684799069381445 28.30153500383282 21.27842508211564 14-15 22.457425756619312 25.824760391641494 27.353907042393377 24.363906809345814 16-17 21.102102190343118 29.237032676308083 28.039066877557822 21.621798255790978 18-19 20.855933036315903 27.32872777308868 31.184601027638426 20.630738162956987 20-21 21.53227759409802 26.118197670942923 31.609000904832495 20.740523830126563 22-23 21.300832037628386 26.21630343864641 31.42881171570587 21.054052808019335 24-25 21.199868479248245 26.28809022850464 31.7272028586005 20.784838433646616 26-27 21.187037037975234 26.372515691502702 31.69645548598442 20.743991784537645 28-29 20.634181583837776 26.894935018350502 31.794194715947206 20.676688681864515 30-31 20.99118570014358 26.476640940137948 31.656144019924636 20.876029339793845 32-33 20.82756202864864 26.58074642094672 31.574651720549628 21.017039829855015 34-35 21.276229249900954 26.411685612758035 31.44389177271175 20.868193364629256 36-37 21.23783782414526 26.395284330836517 31.504145462283006 20.86273238273522 38-39 21.01923413051243 26.78582556292699 31.49260196520986 20.70233834135072 40-41 21.14525884037324 26.678173835595757 31.441625013552247 20.73494231047875 42-43 20.878405938461928 26.865206541428627 31.086682796448066 21.169704723661376 44-45 21.160102986966535 26.79723098404026 30.708182316517735 21.334483712475468 46-47 21.27901568815399 27.26301849283075 30.292535486557714 21.165430332457547 48-49 21.279117013181374 27.36414087015907 30.109795799672316 21.24694631698724 50-51 21.27481069951759 27.467695048144584 30.051483246413348 21.206011005924474 52-53 21.519307990592985 27.780586732703565 29.484468393177583 21.215636883525868 54-55 21.21082394472517 27.554530596611894 29.784998424395827 21.449647034267112 56-57 21.459374236895886 27.75246903760476 29.46187291207115 21.3262838134282 58-59 21.70260496512899 27.43197797599205 29.529558030363056 21.3358590285159 60-61 21.3622541981492 27.65281587317349 29.947574430831992 21.037355497845322 62-63 21.28656440269403 27.569780013233046 29.917886197808745 21.225769386264176 64-65 21.12282315844296 27.583408229416072 29.900407630585164 21.393360981555805 66-67 21.057468515780865 27.72734043081375 29.595368635648374 21.61982241775701 68-69 21.11137343034867 27.599569571283677 29.68580122258778 21.60325577577987 70-71 21.190305626680093 27.649522809783537 29.633264195889648 21.526907367646718 72-73 21.20550438078756 27.444643604414935 29.62769131938358 21.722160695413926 74-75 21.06618246813581 27.892854863044025 29.27117921053618 21.769783458283978 76-77 21.052148951843254 27.599924208879518 29.49586745875818 21.852059380519048 78-79 21.029401483195752 27.74410972284565 29.369920449721004 21.856568344237594 80-81 21.16518774270523 27.900468375176366 29.16353614392214 21.77080773819626 82-83 21.064534645429543 27.821215408146255 29.325238785939483 21.78901116048472 84-85 21.004965959719087 27.769097565251048 29.10426286250442 22.121673612525445 86-87 20.983146608195373 27.572363801431315 29.1734005591115 22.271089031261813 88-89 21.024385894340288 27.504425370570956 29.110275067051838 22.36091366803692 90-91 20.891262580611166 27.334734444223763 29.347733564644983 22.426269410520085 92-93 20.96982734598794 27.131981675713636 29.39257483379556 22.505616144502866 94-95 20.636209714435676 27.167975617145412 29.578498018589087 22.617316649829824 96-97 20.47991585969726 27.25096081457216 29.712095067193694 22.55702825853689 98-99 20.47796077096265 27.31330338162489 29.245978912213932 22.962756935198524 100-101 20.14546073629237 27.360380139552724 29.587842668516423 22.90631645563848 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 462.0 1 333.0 2 303.0 3 425.5 4 359.0 5 310.0 6 559.0 7 1152.0 8 1813.0 9 2138.0 10 1951.5 11 1723.5 12 1768.5 13 1882.0 14 2004.0 15 2123.0 16 2231.0 17 2287.5 18 2370.0 19 2498.5 20 2721.5 21 3074.0 22 3517.5 23 4141.5 24 4894.5 25 5818.5 26 7142.0 27 8744.5 28 10052.0 29 11807.5 30 13906.0 31 15253.5 32 16965.0 33 18994.5 34 21459.0 35 23735.0 36 26320.0 37 29128.0 38 30573.0 39 31521.5 40 31956.5 41 32807.0 42 34016.0 43 35194.5 44 36662.0 45 37256.5 46 36791.5 47 38101.5 48 42440.0 49 41373.0 50 35980.0 51 33004.5 52 30540.5 53 28829.0 54 27227.0 55 24293.0 56 20855.0 57 18363.5 58 16702.5 59 14861.5 60 12427.0 61 9939.0 62 7730.0 63 5668.0 64 4026.5 65 3038.5 66 2268.5 67 1706.5 68 1300.0 69 868.5 70 581.0 71 458.5 72 372.5 73 303.0 74 235.0 75 170.5 76 127.5 77 76.0 78 43.5 79 31.0 80 18.0 81 14.0 82 14.0 83 8.0 84 2.5 85 2.5 86 2.5 87 0.5 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.065151992607326 2 0.0 3 0.0 4 0.0 5 0.0016212004381294183 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.066251369154432E-5 12-13 0.005319563937612154 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.612627327562535E-4 24-25 6.586126779900762E-4 26-27 3.5463759584081027E-4 28-29 0.002938425794109571 30-31 4.053001095323546E-4 32-33 0.0 34-35 0.0 36-37 5.066251369154432E-5 38-39 5.572876506069876E-4 40-41 2.026500547661773E-4 42-43 0.013729541210408513 44-45 2.026500547661773E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.066251369154432E-5 82-83 3.5463759584081027E-4 84-85 4.053001095323546E-4 86-87 0.0 88-89 0.0 90-91 0.008815277382328712 92-93 0.0036477009857911915 94-95 0.0 96-97 0.0 98-99 1.0132502738308865E-4 100-101 0.012918940991343802 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 986923.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.54833670765375 #Duplication Level Percentage of deduplicated Percentage of total 1 83.5397940829662 53.92354756955362 2 9.958404922314138 12.855969479933787 3 2.7075363501677474 5.243009039365189 4 1.1583493374276683 2.99078092229475 5 0.6494945526810314 2.0961896538121083 6 0.40208316402443584 1.5572279673556835 7 0.2690441426859374 1.2156446337919748 8 0.20743972788131287 1.0711911521461643 9 0.14880764628509235 0.8644757451375223 >10 0.8789512232280573 10.378735361432682 >50 0.05513033578994598 2.4256540601013024 >100 0.022295353144412198 2.5276962947269292 >500 0.0017271044379533382 0.7232123138977693 >1k 6.280379774375776E-4 0.8566863353948773 >5k 3.140189887187888E-4 1.269979471055626 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6655 0.674318057234455 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5876 0.5953858609030289 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3975 0.40276698384777737 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1901 0.19261887705525152 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1445 0.14641466456856309 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1132 0.11469993099765637 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 997 0.1010210523009394 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.066251369154432E-5 0.0 12-13 0.0 0.0 0.0 1.0132502738308865E-4 0.0 14-15 0.0 0.0 0.0 1.0132502738308865E-4 0.0 16-17 0.0 0.0 0.0 1.0132502738308865E-4 0.0 18-19 0.0 0.0 0.0 1.0132502738308865E-4 0.0 20-21 0.0 0.0 0.0 1.0132502738308865E-4 0.0 22-23 0.0 0.0 0.0 2.026500547661773E-4 0.0 24-25 0.0 0.0 0.0 4.053001095323546E-4 0.0 26-27 0.0 0.0 0.0 5.066251369154432E-4 0.0 28-29 0.0 0.0 0.0 0.0016212004381294183 0.0 30-31 0.0 0.0 0.0 0.003698363499482736 0.0 32-33 0.0 0.0 0.0 0.00678877683466694 0.0 34-35 0.0 0.0 0.0 0.011044427984756662 0.0 36-37 0.0 0.0 0.0 0.015908029299144918 0.0 38-39 0.0 0.0 0.0 0.02213951848320487 0.0 40-41 0.0 0.0 0.0 0.029080282858946444 0.0 42-43 0.0 0.0 0.0 0.044329699480101285 0.0 44-45 0.0 0.0 0.0 0.060947003970927824 0.0 46-47 0.0 0.0 0.0 0.0780709335986698 1.0132502738308865E-4 48-49 0.0 0.0 0.0 0.10325020290336734 1.0132502738308865E-4 50-51 0.0 0.0 0.0 0.13506626150165718 1.0132502738308865E-4 52-53 0.0 0.0 0.0 0.18638738787119158 1.0132502738308865E-4 54-55 0.0 0.0 0.0 0.2259548110642877 1.0132502738308865E-4 56-57 0.0 0.0 0.0 0.3078760957035148 1.0132502738308865E-4 58-59 0.0 0.0 0.0 0.41462201205159877 1.0132502738308865E-4 60-61 0.0 0.0 0.0 0.5247623168170161 1.0132502738308865E-4 62-63 0.0 0.0 0.0 0.6938231250056995 1.0132502738308865E-4 64-65 0.0 0.0 0.0 0.8146025576463412 1.0132502738308865E-4 66-67 0.0 0.0 0.0 1.0195830880423296 1.5198754107463298E-4 68-69 0.0 0.0 0.0 1.2662588672064587 2.026500547661773E-4 70-71 0.0 0.0 0.0 1.4714420476572134 2.026500547661773E-4 72-73 0.0 0.0 0.0 1.7630048139520509 2.026500547661773E-4 74-75 0.0 0.0 0.0 2.0659159833137943 2.026500547661773E-4 76-77 0.0 0.0 0.0 2.289135018638739 2.5331256845772165E-4 78-79 0.0 0.0 0.0 2.5299339462146486 3.0397508214926596E-4 80-81 1.0132502738308865E-4 0.0 0.0 2.9323462924665855 3.0397508214926596E-4 82-83 2.026500547661773E-4 0.0 0.0 3.2723930843642313 3.0397508214926596E-4 84-85 2.026500547661773E-4 0.0 0.0 3.7004913250577807 3.0397508214926596E-4 86-87 3.0397508214926596E-4 0.0 0.0 4.274649592724053 3.0397508214926596E-4 88-89 4.053001095323546E-4 0.0 0.0 5.010877241689574 3.0397508214926596E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 110 7.014387E-8 34.54356 2 TAACGCA 115 1.03664206E-7 33.041664 4 ATAACGC 125 2.1544292E-7 30.398329 3 GTATACG 80 4.4946308E-4 29.685871 4 GGTATCA 5915 0.0 29.243979 1 GTATCAA 8105 0.0 25.211882 1 GAACCGT 115 1.2820517E-4 24.781248 6 GCGGTAT 135 1.3377852E-5 24.64076 1 GGACCGA 195 1.5701517E-8 24.357635 6 GTACATG 4905 0.0 23.639627 1 TACCTGG 890 0.0 22.94818 2 GAGTACT 5550 0.0 22.597933 12-13 TACATGG 4920 0.0 22.590221 2 GTATAGG 320 0.0 22.275688 1 ACATGGG 4905 0.0 22.078297 3 GTACTTT 5695 0.0 21.642797 14-15 ATCAACG 9470 0.0 21.316147 3 GTACCTG 1005 0.0 21.278267 1 TATCAAC 9605 0.0 21.065996 2 TCAACGC 9585 0.0 21.060398 4 >>END_MODULE