FastQCFastQC Report
Thu 26 May 2016
SRR937255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364485
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44230.3241516029857419No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32870.2408967485901274No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21510.15764189419451297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21430.15705559240299455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.036.0741351
GTATCAA52250.035.382291
ATCAACG61850.029.7971863
TCAACGC62450.029.5902214
CAACGCA63350.029.1719845
TATCAAC64300.028.514092
AACGCAG65300.028.2966846
GTACATG51850.025.8477631
ACGCAGA75000.024.5763557
TACATGG53000.024.3767742
CGCAGAG76050.024.1121068
ACATGGG53950.022.8910183
GCAGAGT80550.022.2932439
GAGTACT41750.021.44677512-13
GGACCGA2457.519702E-921.3268036
GTACTTT43200.020.50474414-15
AGAGTAC68150.020.18039910-11
AGTACTT43550.020.12404812-13
TACCTGG9700.020.076842
CACCGTA1200.004760551719.7941029
Produced by FastQC (version 0.11.5)