##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937255_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1364485 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.573517480954354 31.0 31.0 34.0 30.0 34.0 2 31.6925983063207 33.0 31.0 34.0 30.0 34.0 3 31.7342572472398 33.0 31.0 34.0 30.0 34.0 4 35.35859683323745 37.0 35.0 37.0 33.0 37.0 5 35.36285851438455 37.0 35.0 37.0 33.0 37.0 6 35.39109627441855 37.0 35.0 37.0 33.0 37.0 7 35.32292256785527 37.0 35.0 37.0 33.0 37.0 8 35.29932465362389 37.0 35.0 37.0 33.0 37.0 9 36.87834384401441 39.0 37.0 39.0 33.0 39.0 10-11 36.75679981824644 39.0 37.0 39.0 33.0 39.0 12-13 36.59663499415531 39.0 37.0 39.0 32.0 39.0 14-15 37.675951732705016 40.0 37.0 41.0 32.0 41.0 16-17 37.579810697809066 40.0 37.0 41.0 32.0 41.0 18-19 37.47389821068022 40.0 37.0 41.0 32.0 41.0 20-21 37.3238569863355 40.0 37.0 41.0 32.0 41.0 22-23 37.40445955800173 40.0 37.0 41.0 32.0 41.0 24-25 37.36933934781254 40.0 37.0 41.0 32.0 41.0 26-27 37.27918335489214 40.0 37.0 41.0 31.5 41.0 28-29 37.12326592084193 39.5 37.0 41.0 31.0 41.0 30-31 36.874800382561915 39.0 36.0 41.0 30.5 41.0 32-33 36.6448733404911 39.0 36.0 40.5 30.0 41.0 34-35 36.63790954096234 39.0 36.0 41.0 30.0 41.0 36-37 36.49990289376578 39.0 35.5 41.0 30.0 41.0 38-39 36.3459374782427 39.0 35.0 41.0 30.0 41.0 40-41 36.10429905788631 39.0 35.0 40.5 29.5 41.0 42-43 35.8302912820588 39.0 35.0 40.0 28.5 41.0 44-45 35.59418242047366 38.5 35.0 40.0 27.5 41.0 46-47 35.215872288812264 38.0 34.5 40.0 26.5 41.0 48-49 34.87857579966068 38.0 34.0 40.0 24.5 41.0 50-51 33.924070620050784 37.5 33.0 39.5 23.5 40.5 52-53 34.03944235370854 38.0 33.5 39.5 24.0 40.0 54-55 34.31800716021063 38.0 34.0 40.0 23.5 41.0 56-57 34.00708435783464 38.0 33.0 40.0 22.5 41.0 58-59 33.69979699300469 38.0 33.0 40.0 21.5 41.0 60-61 33.33305093130375 37.0 32.5 40.0 20.0 41.0 62-63 32.89787135805817 37.0 32.0 39.5 18.0 41.0 64-65 32.43213336900003 36.0 31.0 39.0 15.5 41.0 66-67 31.95730367134853 35.5 31.0 39.0 11.5 40.0 68-69 31.428315811460003 35.0 30.5 38.5 8.0 40.0 70-71 30.918476201643845 35.0 30.0 38.0 7.0 40.0 72-73 30.376134219137626 34.5 29.0 37.0 2.0 39.0 74-75 29.773403884982244 34.0 29.0 36.5 2.0 39.0 76-77 29.192146121063992 34.0 28.0 36.0 2.0 39.0 78-79 28.593095929966253 34.0 27.5 35.5 2.0 37.5 80-81 27.940320340641343 33.5 26.0 35.0 2.0 37.0 82-83 27.319109774017303 33.0 26.0 35.0 2.0 36.5 84-85 26.666948335819008 32.5 24.5 35.0 2.0 36.0 86-87 25.989334071096422 32.0 23.0 35.0 2.0 36.0 88-89 25.349899779037514 31.5 20.0 34.0 2.0 35.0 90-91 24.68704969274122 31.0 18.0 34.0 2.0 35.0 92-93 23.917354899467565 31.0 8.5 34.0 2.0 35.0 94-95 23.04657874582718 30.5 2.0 34.0 2.0 35.0 96-97 22.09777351894671 30.0 2.0 34.0 2.0 35.0 98-99 20.825042048831612 29.0 2.0 34.0 2.0 35.0 100-101 17.832207389601205 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3341456679717112 1101 2 0.29259957298370765 1101 3 0.32618809800633386 1101 4 0.11033841857342708 1101 5 0.09272229777923258 1101 6 0.25885029003772786 1101 7 0.26917024357751274 1101 8 0.19845916710849565 1101 9 0.33188170088818936 1101 10-11 0.37488948147315426 1101 12-13 0.37075370098006744 1101 14-15 0.5433074650866345 1101 16-17 0.4993528963020779 1101 18-19 0.6373870924006866 1101 20-21 0.5835844898951521 1101 22-23 0.6353231156514241 1101 24-25 0.6236500893520756 1101 26-27 0.6106032768654828 1101 28-29 0.6341815054747926 1101 30-31 0.6503067361265025 1101 32-33 0.6332022589622497 1101 34-35 0.6182741913284318 1101 36-37 0.7443315672336439 1101 38-39 0.5026134573403311 1101 40-41 0.7880126044204872 1101 42-43 0.7886259596007648 1101 44-45 0.9170356530747839 1101 46-47 0.9950292532925431 1101 48-49 0.8991934125688417 1101 50-51 1.0033926409504659 1101 52-53 0.888013967061319 1101 54-55 1.0106748289829142 1101 56-57 1.1313315009050768 1101 58-59 0.9656096877942559 1101 60-61 0.7987096422238764 1101 62-63 1.041156383229378 1101 64-65 0.946832975106382 1101 66-67 1.1585913841758462 1101 68-69 1.0670759951156477 1101 70-71 1.0358837150095006 1101 72-73 1.1700494581145158 1101 74-75 1.309744903505507 1101 76-77 1.2519403133049067 1101 78-79 1.3802173945701917 1101 80-81 1.4194511281694595 1101 82-83 1.4284253357684946 1101 84-85 1.5634227260657703 1101 86-87 1.607765541566721 1101 88-89 1.567744516842776 1101 90-91 1.6060428729824636 1101 92-93 1.8440584636033215 1101 94-95 1.7587744504033367 1101 96-97 1.713809814263751 1101 98-99 1.8933088309657613 1101 100-101 1.5954925557068123 1102 1 0.29205046294371684 1102 2 0.28716706856953067 1102 3 0.19629633878667008 1102 4 0.16942131472352884 1102 5 0.05089102580539162 1102 6 -0.0728429887023978 1102 7 -0.14546293887644168 1102 8 -0.030375025065815464 1102 9 0.13068567484491211 1102 10-11 0.1538328459562308 1102 12-13 0.21523079596632755 1102 14-15 0.45407127605411546 1102 16-17 0.4066799319386831 1102 18-19 0.31661033883370493 1102 20-21 0.41245072100171143 1102 22-23 0.3450440382033051 1102 24-25 0.2645959858987865 1102 26-27 0.4007235710590962 1102 28-29 0.41031546490813753 1102 30-31 0.4455666678426482 1102 32-33 0.45384704600214576 1102 34-35 0.5746589012772958 1102 36-37 0.6573515118218154 1102 38-39 0.5927211900142524 1102 40-41 0.7171338767189468 1102 42-43 0.7535828481829085 1102 44-45 0.5883409078830937 1102 46-47 0.498736313007754 1102 48-49 0.46753160819827855 1102 50-51 0.29054333543313504 1102 52-53 0.4274924240550533 1102 54-55 0.6901895309008665 1102 56-57 0.7725243936254671 1102 58-59 0.7583940121468089 1102 60-61 0.8269102539275437 1102 62-63 0.8910913879505387 1102 64-65 0.5552657067570337 1102 66-67 0.7553087344573477 1102 68-69 0.8540530865733906 1102 70-71 0.8277174569450771 1102 72-73 0.7242065448581592 1102 74-75 0.830900478632568 1102 76-77 0.9758853926471325 1102 78-79 1.061556339656594 1102 80-81 1.1499691285448925 1102 82-83 1.3836343439306589 1102 84-85 1.3065791677965528 1102 86-87 1.34286143959541 1102 88-89 1.233140211770042 1102 90-91 1.2166915131626723 1102 92-93 1.5806815036886803 1102 94-95 1.7405482498960758 1102 96-97 1.6079289059388486 1102 98-99 1.9940388105144784 1102 100-101 1.6966403116407172 1103 1 0.2593897844536599 1103 2 0.21877042126860147 1103 3 0.23246192291715317 1103 4 0.0570202560758446 1103 5 0.06996122494861368 1103 6 0.11021609898350704 1103 7 0.11406531517634022 1103 8 0.12301558401539836 1103 9 0.12608218145862793 1103 10-11 0.13823167249267243 1103 12-13 0.01390219878781096 1103 14-15 0.1281990861395954 1103 16-17 0.14799768959221637 1103 18-19 0.13843068975860717 1103 20-21 0.04633136536804017 1103 22-23 -0.07387008311062715 1103 24-25 0.07629110631854275 1103 26-27 0.001383620705631472 1103 28-29 -0.08571248180445679 1103 30-31 0.01755083911469768 1103 32-33 0.13733881768850864 1103 34-35 0.35207638444912703 1103 36-37 0.23327704957272744 1103 38-39 0.259656956386614 1103 40-41 0.21208589671353195 1103 42-43 -0.09035891486394831 1103 44-45 0.2469692830779593 1103 46-47 0.051014360042600515 1103 48-49 0.2738848634077087 1103 50-51 0.3100717122331602 1103 52-53 0.23234037327924284 1103 54-55 0.5329366328858782 1103 56-57 0.3790161171571498 1103 58-59 0.31844101489540577 1103 60-61 0.19624794762032138 1103 62-63 0.1598450408610148 1103 64-65 0.1675421297515598 1103 66-67 0.7159668295902328 1103 68-69 0.4574657429462867 1103 70-71 0.5374053768232372 1103 72-73 0.5112903727212164 1103 74-75 0.4856649125270458 1103 76-77 0.46660522963880524 1103 78-79 0.4193791625219454 1103 80-81 0.22872871109099435 1103 82-83 0.45276204543204557 1103 84-85 0.6392607605268914 1103 86-87 0.9720682469681741 1103 88-89 0.8886035967421897 1103 90-91 0.8809634285010723 1103 92-93 0.7742803298474392 1103 94-95 0.8497975372539592 1103 96-97 0.9612556392478204 1103 98-99 0.906767887540255 1103 100-101 0.6923295231974649 1104 1 0.22155427908205283 1104 2 0.3425080802823608 1104 3 0.25919956896720464 1104 4 0.2132171858995946 1104 5 0.25265504626057833 1104 6 0.23320741885557794 1104 7 0.23593620035678242 1104 8 0.2899084982382547 1104 9 0.24698727045448265 1104 10-11 0.40045830031760943 1104 12-13 0.4305400057730324 1104 14-15 0.539196291534715 1104 16-17 0.40184003980120053 1104 18-19 0.48498484379337725 1104 20-21 0.5114978841867099 1104 22-23 0.5490207680308359 1104 24-25 0.5169298281710937 1104 26-27 0.5913508225264721 1104 28-29 0.5840810618989778 1104 30-31 0.46745398170250496 1104 32-33 0.5825426164251084 1104 34-35 0.6334212394315486 1104 36-37 0.6561008598284985 1104 38-39 0.8207113872891298 1104 40-41 0.8466376024596016 1104 42-43 0.6933309821486588 1104 44-45 0.7034232646954166 1104 46-47 0.9371879181542724 1104 48-49 1.1056418716804117 1104 50-51 1.060547665482325 1104 52-53 0.9038141060305662 1104 54-55 1.117966977220462 1104 56-57 1.1413770119492597 1104 58-59 1.3019724768867391 1104 60-61 1.3208208397678334 1104 62-63 1.156564759955998 1104 64-65 1.0788694466901205 1104 66-67 1.4265877170856278 1104 68-69 1.2710777280787617 1104 70-71 1.3289461874994082 1104 72-73 1.4064593546442516 1104 74-75 1.36428479626111 1104 76-77 1.5446698963141152 1104 78-79 1.5457882711930004 1104 80-81 1.6194872546370114 1104 82-83 1.4582155992471755 1104 84-85 1.527170992410575 1104 86-87 1.5236550047264892 1104 88-89 1.6360765998219975 1104 90-91 1.8016074839931449 1104 92-93 1.8679088529663588 1104 94-95 1.6822656974631585 1104 96-97 1.6441295634916209 1104 98-99 2.0250806980904876 1104 100-101 1.8856600662471585 1105 1 0.1521075290370213 1105 2 0.01286625876763381 1105 3 0.12931615975508493 1105 4 0.1552520324525588 1105 5 0.1328386741025014 1105 6 0.1856192340480476 1105 7 0.17270574194059662 1105 8 0.2510734524813998 1105 9 0.2352081042145926 1105 10-11 0.20451884558527667 1105 12-13 0.2646409569362831 1105 14-15 0.23398122903626017 1105 16-17 0.3207946961921522 1105 18-19 0.1930840360286652 1105 20-21 9.650667584892858E-4 1105 22-23 0.2425231538859478 1105 24-25 0.13016788035262294 1105 26-27 0.27224270143593543 1105 28-29 0.3720450321314175 1105 30-31 0.13919184139091811 1105 32-33 0.1953184202163385 1105 34-35 0.3578642455736656 1105 36-37 0.2848840560619976 1105 38-39 0.11158482924273727 1105 40-41 0.06578042529176287 1105 42-43 0.22811086891325516 1105 44-45 0.053786794136271965 1105 46-47 0.2744817827450703 1105 48-49 0.36306477730227016 1105 50-51 0.2798790079540723 1105 52-53 -0.020366562008867106 1105 54-55 0.12760973471091575 1105 56-57 0.23297857237973574 1105 58-59 0.21870728140908824 1105 60-61 0.13751529137435625 1105 62-63 -0.10623470812722502 1105 64-65 0.20085357395456782 1105 66-67 0.08559630687042485 1105 68-69 0.15530812645122793 1105 70-71 0.2640362707482673 1105 72-73 0.15999796099405117 1105 74-75 0.22145004365892262 1105 76-77 0.2516445931815987 1105 78-79 0.17757144623459098 1105 80-81 0.21312809426022028 1105 82-83 0.5435112439578518 1105 84-85 0.7612236436942759 1105 86-87 0.5409387678433966 1105 88-89 0.7785272491772339 1105 90-91 0.7692624586158416 1105 92-93 0.9703399174709801 1105 94-95 1.042537324683451 1105 96-97 0.9464011589240577 1105 98-99 1.2456937092127092 1105 100-101 0.9076475488962892 1106 1 -0.04085214820257477 1106 2 -0.6090103433320913 1106 3 -0.19925638332050255 1106 4 0.015790412260713538 1106 5 -0.015254780786086997 1106 6 -0.1264493599678076 1106 7 -0.09646187205460421 1106 8 -0.07007277789404043 1106 9 0.04139211628096007 1106 10-11 -0.3207980450967227 1106 12-13 -0.3298467055026961 1106 14-15 -0.265360284757584 1106 16-17 -0.20425099847828676 1106 18-19 -0.11821369261186732 1106 20-21 -0.06677263840315106 1106 22-23 -0.18467650581878559 1106 24-25 -0.24736297283745756 1106 26-27 -0.048832014922759015 1106 28-29 0.06756899768581093 1106 30-31 -0.07252865582653811 1106 32-33 0.01159519617871041 1106 34-35 0.12221162908939931 1106 36-37 -0.11924629634421535 1106 38-39 0.03649696916502165 1106 40-41 0.19344130396683568 1106 42-43 0.23019406293357036 1106 44-45 0.06362786584347191 1106 46-47 0.24224196374055396 1106 48-49 0.4656632437445509 1106 50-51 0.18092311259858462 1106 52-53 0.22339500097176312 1106 54-55 0.6311492141631803 1106 56-57 0.21922466232765458 1106 58-59 0.04167015356060233 1106 60-61 -0.1452817797087178 1106 62-63 0.07093430271318368 1106 64-65 0.21758878262689763 1106 66-67 0.2748502577876657 1106 68-69 0.35078844059279746 1106 70-71 0.45679719474651037 1106 72-73 0.47965261330590536 1106 74-75 0.4721531100313605 1106 76-77 0.4701227006049393 1106 78-79 0.6651920913095957 1106 80-81 0.5789213611691046 1106 82-83 0.5562184488557236 1106 84-85 0.42593342681176694 1106 86-87 0.5406099891170477 1106 88-89 0.7783089921131356 1106 90-91 0.5979947472872809 1106 92-93 0.8930620567245597 1106 94-95 0.7438399942924079 1106 96-97 0.5516818653710907 1106 98-99 0.48228808170971504 1106 100-101 0.2850966717815169 1107 1 0.19018354764227396 1107 2 0.28602565891485554 1107 3 0.2649555674962869 1107 4 0.36847719933205525 1107 5 0.2843166345457462 1107 6 0.2927418141385729 1107 7 0.32893441745377316 1107 8 0.27267107184032113 1107 9 0.2312346974727859 1107 10-11 0.2646841221118166 1107 12-13 0.320894617360004 1107 14-15 0.4645339686899135 1107 16-17 0.4136613251277197 1107 18-19 0.419422698879238 1107 20-21 0.5247269256358393 1107 22-23 0.5260257696896602 1107 24-25 0.5961530291068726 1107 26-27 0.5324397240226588 1107 28-29 0.49723174732314135 1107 30-31 0.4526447828939837 1107 32-33 0.39788313471671444 1107 34-35 0.5945217212559086 1107 36-37 0.676210441381258 1107 38-39 0.5675175813261575 1107 40-41 0.5659922149456804 1107 42-43 0.4962896301273645 1107 44-45 0.6735659329001322 1107 46-47 0.5026929706119034 1107 48-49 0.46102465637171974 1107 50-51 0.34692117961741786 1107 52-53 0.39120476498540313 1107 54-55 0.5029133148068183 1107 56-57 0.5782622003678526 1107 58-59 0.7494564078327315 1107 60-61 0.8473142120978636 1107 62-63 0.7494449709912985 1107 64-65 0.5668287824755822 1107 66-67 0.7414105821199577 1107 68-69 0.8924700018060712 1107 70-71 0.679955330384292 1107 72-73 0.739560796700804 1107 74-75 0.9129896066826895 1107 76-77 0.8750801258437697 1107 78-79 0.947340197006973 1107 80-81 0.9976193602877252 1107 82-83 0.8973172786644881 1107 84-85 0.9705674181530703 1107 86-87 0.9711744498777222 1107 88-89 1.2693618616066438 1107 90-91 1.2156635568772884 1107 92-93 1.7911828850694036 1107 94-95 1.6665576296247337 1107 96-97 1.4868780572847236 1107 98-99 1.3450364896481766 1107 100-101 1.0784015021539055 1108 1 0.08014466431553657 1108 2 0.13344981314429205 1108 3 0.15769812261980576 1108 4 0.12671091839420257 1108 5 0.11604822317411845 1108 6 0.08397467171383255 1108 7 0.1476924793411314 1108 8 -0.046035300834248005 1108 9 0.08396141986446537 1108 10-11 0.2669856890866029 1108 12-13 0.28572849009278656 1108 14-15 0.2533048364633146 1108 16-17 0.4173862081284909 1108 18-19 0.47897263019311964 1108 20-21 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0.05986323689931794 2316 46-47 0.17061997822202812 2316 48-49 -0.03704241634248007 2316 50-51 0.03535033618384631 2316 52-53 0.2120284502823182 2316 54-55 0.12171003666104951 2316 56-57 0.268344717267226 2316 58-59 0.1697533807477143 2316 60-61 -0.05456843435023018 2316 62-63 0.16326691225751233 2316 64-65 0.3608088885736507 2316 66-67 0.3376777794372181 2316 68-69 0.4297434203466537 2316 70-71 0.617073716341924 2316 72-73 0.5844014813169949 2316 74-75 0.9043669925324771 2316 76-77 1.0320112912395665 2316 78-79 0.968157631543999 2316 80-81 0.9338835902112841 2316 82-83 1.1966342285959044 2316 84-85 1.5253439947011813 2316 86-87 1.635528220906206 2316 88-89 1.6464554368558346 2316 90-91 1.7585248799914304 2316 92-93 2.2404287755382306 2316 94-95 2.284705577555215 2316 96-97 2.1721952283968733 2316 98-99 2.599900233386254 2316 100-101 3.292484876300353 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6154.0 3 1297.0 4 1287.0 5 1489.0 6 1685.0 7 1929.0 8 2405.0 9 3120.0 10 3805.0 11 4841.0 12 6010.0 13 7412.0 14 8354.0 15 9884.0 16 11806.0 17 14540.0 18 13649.0 19 11297.0 20 11808.0 21 13161.0 22 14383.0 23 16318.0 24 18386.0 25 20851.0 26 24116.0 27 27640.0 28 32477.0 29 37956.0 30 45166.0 31 54321.0 32 66334.0 33 83619.0 34 107298.0 35 140479.0 36 182736.0 37 213578.0 38 131781.0 39 11113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42102361845906 19.1216614506235 14.551878437039248 22.90543649387819 2 15.153062498989772 19.99393128094055 40.72213348536968 24.130872734699995 3 19.271840522891186 24.38686952995045 29.54127995485831 26.80000999230005 4 12.485452728715432 16.47000911041232 37.58368354541982 33.46085461545243 5 13.691804357872478 37.28194657613052 34.30254129634106 14.723707769655942 6 31.8057022485527 36.331746355852154 17.663338469832492 14.199212925762653 7 28.68402670918905 32.318676968208834 20.73943116272818 18.25786515987393 8 26.354270033559736 35.298390763397634 19.62528269429793 18.722056508744704 9 26.034528860732713 17.03127020133755 20.310057474641813 36.62414346328793 10-11 24.808295560567437 27.012914980054507 27.364660853211483 20.814128606166573 12-13 25.721657909670405 24.0151914448952 28.086248914175314 22.17690173125908 14-15 22.530695530307703 25.57252048572243 26.25125235413196 25.645531629837908 16-17 22.120239602234733 28.996262595881838 26.748678761357365 22.134819040526065 18-19 21.977118902976393 27.549888076801054 28.98895309213541 21.484039928087142 20-21 22.622198311872165 26.716413251638276 29.128816602922736 21.532571833566827 22-23 22.19165798211386 26.63341199004416 29.064389073841102 22.11054095400088 24-25 22.074819777192427 26.850086131647938 29.13728440923531 21.937809681924325 26-27 22.08950751697755 27.053591559070572 29.00240223082126 21.85449869313062 28-29 21.789889841915162 27.157759819016825 29.144975678549194 21.907374660518823 30-31 22.0233374997383 26.864753940301284 29.15178336946952 21.9601251904909 32-33 21.885049382915554 26.991623341232053 29.075320882443243 22.048006393409146 34-35 22.26054023997852 27.005167790682254 28.8186138891674 21.915678080171823 36-37 22.113740229551603 26.960763972406802 28.89001096163062 22.035484836410976 38-39 22.005475500513327 27.056690817814765 29.001536312644028 21.93629736902788 40-41 22.214123866653498 26.9593726511964 28.922903614187977 21.903599867962125 42-43 22.07720623337402 26.93885899701375 28.750181774916385 22.233752994695845 44-45 22.104055633035824 27.122353461311643 28.58078180895496 22.192809096697577 46-47 22.163944781288215 27.313027223750364 28.231576318679096 22.29145167628232 48-49 22.21337948044832 27.323362050412687 28.18267088669889 22.280587582440102 50-51 22.212525412803092 27.429298689277864 28.151527339309357 22.206648558609686 52-53 22.39731492147828 27.524846600526615 27.87792987116795 22.199908606827158 54-55 22.214089359068765 27.296806453567697 28.056602768805305 22.432501418558232 56-57 22.23716672743609 27.65827681409586 27.94762006000568 22.156936398462367 58-59 22.37734431707911 27.4170317084248 27.987991163364313 22.217632811131782 60-61 22.355661200356 27.362725400867678 28.302991050054487 21.978622348721835 62-63 22.278543944957867 27.414950424848566 28.228076172753514 22.078429457440055 64-65 22.094962344620157 27.490593498131915 28.10675241440891 22.307691742839015 66-67 22.103777083417476 27.611849327894312 27.899931339107702 22.38444224958051 68-69 21.97124495178424 27.48472821790594 28.112709919305658 22.431316911004163 70-71 22.117420356416098 27.44056610632265 28.070833789250827 22.371179748010427 72-73 22.193956796492607 27.34455953792883 28.02066542067056 22.440818244908 74-75 21.973659280278827 27.668808116948444 27.836320054285324 22.521212548487398 76-77 22.133072162707244 27.549204516577287 27.79212404576508 22.525599274950387 78-79 22.124580811100223 27.543005880028616 27.85815954957482 22.474253759296335 80-81 22.13322464147292 27.78163952124173 27.727293591035856 22.357842246249486 82-83 22.202271846029117 27.642262093599822 27.77470566463393 22.38076039573713 84-85 22.28113175820795 27.424936539976695 27.759106706092528 22.534824995722825 86-87 22.233750822881408 27.379545398638083 27.90953059136214 22.477173187118368 88-89 22.282341295193948 27.219656880671963 27.8531469250416 22.644854899092486 90-91 22.330995774917323 27.360889327214238 27.798782227408363 22.509332670460076 92-93 22.402748096087894 27.170863718815863 27.88447274302878 22.541915442067463 94-95 22.19404737120195 27.140900080008258 27.840999842480308 22.824052706309487 96-97 22.134807819486586 27.146817150815977 27.942065854685804 22.77630917501163 98-99 22.13524979500318 27.147637735278145 27.735509488240545 22.98160298147813 100-101 22.62047999236301 26.479621528243108 28.161118525766593 22.738779953627297 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1954.0 1 1090.0 2 234.0 3 241.5 4 212.0 5 190.5 6 309.0 7 604.0 8 1010.0 9 1272.0 10 1204.0 11 1117.5 12 1174.0 13 1280.0 14 1416.0 15 1516.5 16 1552.5 17 1648.5 18 1837.5 19 1987.5 20 2131.5 21 2466.0 22 2986.5 23 3578.0 24 4260.0 25 5335.0 26 6984.0 27 9186.0 28 11184.5 29 13510.0 30 16359.5 31 18739.5 32 21221.5 33 24033.0 34 27320.5 35 30298.5 36 33445.5 37 36900.0 38 39177.5 39 40640.0 40 42301.5 41 44898.5 42 47164.0 43 49210.0 44 51464.0 45 52934.5 46 53226.5 47 53594.0 48 55563.0 49 55452.5 50 52913.0 51 50413.5 52 47604.0 53 45388.5 54 43049.5 55 39416.5 56 35317.0 57 31287.5 58 27940.5 59 24813.0 60 20876.5 61 16864.0 62 13227.0 63 9877.0 64 7436.0 65 5818.0 66 4329.5 67 3264.0 68 2391.0 69 1583.5 70 1131.0 71 849.0 72 644.5 73 460.5 74 336.0 75 267.5 76 193.0 77 111.5 78 66.0 79 54.0 80 34.5 81 23.0 82 12.5 83 6.5 84 9.0 85 7.5 86 5.0 87 4.5 88 5.0 89 4.5 90 2.5 91 1.5 92 0.5 93 1.5 94 2.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.288680344598878 2 0.2496912754629036 3 0.2518899071810976 4 0.24954470001502396 5 0.2497645631868434 6 0.21891043140818697 7 0.2203028981630432 8 0.22220837898547802 9 0.23371455164402688 10-11 0.24001729590284981 12-13 0.23397105867781617 14-15 0.22345427029245465 16-17 0.21828748575469867 18-19 0.20791727281721673 20-21 0.2138169345943708 22-23 0.21048234315510977 24-25 0.21301076963103296 26-27 0.20769740964539735 28-29 0.22224502284744793 30-31 0.23448407274539476 32-33 0.2259460529064079 34-35 0.2251032440811002 36-37 0.2138169345943708 38-39 0.20395973572446746 40-41 0.20187103559218314 42-43 0.21334056438876203 44-45 0.20872343778055458 46-47 0.21913029458000638 48-49 0.22294125622487607 50-51 0.23543681315661222 52-53 0.2442679838913583 54-55 0.22481009318534098 56-57 0.20377651641461797 58-59 0.194542263198203 60-61 0.19707068967412616 62-63 0.20260391283158113 64-65 0.19501863340381168 66-67 0.19934260911626 68-69 0.2009182951809657 70-71 0.20242069352173164 72-73 0.2044727497920461 74-75 0.20454603751598588 76-77 0.21422001707603966 78-79 0.21451316797179887 80-81 0.20795391667918664 82-83 0.20322685848506947 84-85 0.190841233139243 86-87 0.194542263198203 88-89 0.20102822676687543 90-91 0.2005518565612667 92-93 0.2063049428905411 94-95 0.20157788469642393 96-97 0.20780734123130706 98-99 0.21095871336071853 100-101 0.19732719670791546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1364485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.19068460275533 #Duplication Level Percentage of deduplicated Percentage of total 1 84.04536018691708 56.47065288644115 2 9.763249890464792 13.119988881762104 3 2.6615483880590203 5.364937748911363 4 1.1424591666906303 3.0705045416254726 5 0.600916425034592 2.018799299355727 6 0.36738583564092625 1.4810943486041508 7 0.2489050774619608 1.1706871789039694 8 0.17596487237723754 0.9458560192850449 9 0.14001749192448681 0.8467084024890329 >10 0.7779772916102686 9.557574578710392 >50 0.05506515796789437 2.5347744708985824 >100 0.020169046454878607 2.2766662130897855 >500 5.450941090006384E-4 0.26245651539198195 >1k 4.360752872005107E-4 0.8792989145312171 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4423 0.3241516029857419 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3287 0.2408967485901274 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2151 0.15764189419451297 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2143 0.15705559240299455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.328772393980146E-5 0.0 16-17 0.0 0.0 0.0 7.328772393980146E-5 0.0 18-19 0.0 0.0 0.0 7.328772393980146E-5 0.0 20-21 0.0 0.0 0.0 7.328772393980146E-5 0.0 22-23 0.0 0.0 0.0 7.328772393980146E-5 0.0 24-25 0.0 0.0 0.0 7.328772393980146E-5 0.0 26-27 0.0 0.0 0.0 2.565070337893051E-4 0.0 28-29 0.0 0.0 0.0 7.328772393980147E-4 0.0 30-31 0.0 0.0 0.0 0.001612329926675632 0.0 32-33 0.0 0.0 0.0 0.0027116457857726543 0.0 34-35 0.0 0.0 0.0 0.00454383888426769 0.0 36-37 0.0 0.0 0.0 0.006925689912311238 0.0 38-39 0.0 0.0 0.0 0.008684595286866473 0.0 40-41 0.0 0.0 0.0 0.012348981483856547 0.0 42-43 0.0 0.0 0.0 0.01747912215964265 0.0 44-45 0.0 0.0 0.0 0.024331524348014087 0.0 46-47 0.0 0.0 0.0 0.041810646507656736 0.0 48-49 0.0 0.0 0.0 0.07863772778740696 0.0 50-51 0.0 0.0 0.0 0.10696343309014023 0.0 52-53 0.0 0.0 0.0 0.13213776626346202 0.0 54-55 0.0 0.0 0.0 0.16420114548712517 0.0 56-57 0.0 0.0 0.0 0.19197719286030993 0.0 58-59 0.0 0.0 0.0 0.22832790393445146 0.0 60-61 0.0 0.0 0.0 0.285345753159617 0.0 62-63 0.0 0.0 0.0 0.33730674943293626 0.0 64-65 0.0 0.0 0.0 0.39666980582417544 0.0 66-67 0.0 0.0 0.0 0.4955349454189676 0.0 68-69 0.0 0.0 0.0 0.6051000927089708 0.0 70-71 0.0 0.0 0.0 0.7045515340952814 0.0 72-73 0.0 0.0 0.0 0.8108187338079935 0.0 74-75 0.0 0.0 0.0 0.9123955191885583 0.0 76-77 0.0 0.0 0.0 1.0076329164483302 0.0 78-79 0.0 0.0 0.0 1.1247466993041328 0.0 80-81 0.0 0.0 0.0 1.3085156670831852 0.0 82-83 0.0 0.0 0.0 1.505696288343221 0.0 84-85 0.0 0.0 0.0 1.6986628654767184 0.0 86-87 0.0 0.0 0.0 1.9736750495608233 0.0 88-89 0.0 0.0 0.0 2.3306229090096267 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3175 0.0 36.074135 1 GTATCAA 5225 0.0 35.38229 1 ATCAACG 6185 0.0 29.797186 3 TCAACGC 6245 0.0 29.590221 4 CAACGCA 6335 0.0 29.171984 5 TATCAAC 6430 0.0 28.51409 2 AACGCAG 6530 0.0 28.296684 6 GTACATG 5185 0.0 25.847763 1 ACGCAGA 7500 0.0 24.576355 7 TACATGG 5300 0.0 24.376774 2 CGCAGAG 7605 0.0 24.112106 8 ACATGGG 5395 0.0 22.891018 3 GCAGAGT 8055 0.0 22.293243 9 GAGTACT 4175 0.0 21.446775 12-13 GGACCGA 245 7.519702E-9 21.326803 6 GTACTTT 4320 0.0 20.504744 14-15 AGAGTAC 6815 0.0 20.180399 10-11 AGTACTT 4355 0.0 20.124048 12-13 TACCTGG 970 0.0 20.07684 2 CACCGTA 120 0.0047605517 19.794102 9 >>END_MODULE