FastQCFastQC Report
Thu 26 May 2016
SRR937255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364485
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50650.37120232175509443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42810.31374474618629006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29310.21480631886755808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39150.036.658191
TACCGCG651.3272541E-436.5434278
GTATCAA62050.033.0089231
ATCAACG74600.027.4417293
TCAACGC75000.027.2953724
TACCGTA700.00839405227.1415712
TATCAAC76250.026.9724922
CAACGCA76000.026.9362225
AACGCAG78050.026.4113046
ATAGGCG908.9508883E-426.3876365
GTACATG50100.023.4290711
ACGCAGA88100.023.0252597
CGCAGAG88750.022.7495698
GAGTACT48550.022.26100512-13
TACATGG51900.021.781242
GCAGAGT95300.021.0862839
GTACTTT51250.020.85266914-15
ACATGGG51700.020.5792943
AGTACTT51400.020.42592412-13
TACCTGG10250.020.3892772
Produced by FastQC (version 0.11.5)