##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937255_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1364485 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1831782687241 33.0 31.0 34.0 31.0 34.0 2 32.38771844322217 34.0 31.0 34.0 31.0 34.0 3 32.46359542244876 34.0 31.0 34.0 31.0 34.0 4 36.09690542585664 37.0 35.0 37.0 35.0 37.0 5 36.01820027336321 37.0 35.0 37.0 35.0 37.0 6 36.05310721627574 37.0 35.0 37.0 35.0 37.0 7 35.99425497532036 37.0 35.0 37.0 35.0 37.0 8 35.96887030637933 37.0 35.0 37.0 35.0 37.0 9 37.66333451815154 39.0 37.0 39.0 35.0 39.0 10-11 37.58080154783673 39.0 37.0 39.0 35.0 39.0 12-13 37.466086105746854 39.0 37.0 39.0 35.0 39.0 14-15 38.69697504919438 40.0 38.0 41.0 34.5 41.0 16-17 38.64542299842065 40.0 38.0 41.0 34.0 41.0 18-19 38.60942663349175 40.0 38.0 41.0 34.0 41.0 20-21 38.497934385500756 40.0 38.0 41.0 34.0 41.0 22-23 38.44509906668084 40.0 38.0 41.0 34.0 41.0 24-25 38.48691081250435 40.0 38.0 41.0 34.0 41.0 26-27 38.33312531834355 40.0 38.0 41.0 34.0 41.0 28-29 38.179857968391005 40.0 38.0 41.0 34.0 41.0 30-31 38.24840947317119 40.0 38.0 41.0 34.0 41.0 32-33 38.165822269940676 40.0 38.0 41.0 34.0 41.0 34-35 38.038189133629174 40.0 37.0 41.0 33.0 41.0 36-37 37.88220793925913 40.0 37.0 41.0 33.0 41.0 38-39 37.78009798568691 40.0 37.0 41.0 33.0 41.0 40-41 37.589443636243715 40.0 37.0 41.0 33.0 41.0 42-43 37.40610853179038 40.0 37.0 41.0 32.5 41.0 44-45 37.12830591761727 40.0 36.5 41.0 31.5 41.0 46-47 36.80400114328849 39.0 36.0 41.0 31.0 41.0 48-49 36.50467539034874 39.0 36.0 41.0 30.0 41.0 50-51 36.260739766285454 39.0 35.5 40.0 30.0 41.0 52-53 36.01530540826759 39.0 35.0 40.0 29.5 41.0 54-55 35.79743383034625 39.0 35.0 40.0 29.0 41.0 56-57 35.49103837711664 38.0 35.0 40.0 28.0 41.0 58-59 35.2178781738165 38.0 34.0 40.0 28.0 41.0 60-61 34.86592487275419 38.0 34.0 40.0 27.0 41.0 62-63 34.47061015694566 37.0 34.0 40.0 26.0 41.0 64-65 34.04467876158404 37.0 33.0 39.0 26.0 41.0 66-67 33.58458356083064 36.0 33.0 39.0 25.5 40.0 68-69 33.12228752972733 36.0 32.0 39.0 24.0 40.0 70-71 32.66975598852314 35.0 32.0 38.0 23.0 40.0 72-73 32.172971487411004 35.0 31.0 37.5 22.0 39.5 74-75 31.665760341813943 35.0 31.0 37.0 20.5 39.0 76-77 30.35773240453358 33.5 29.5 35.5 20.0 38.0 78-79 31.020330014620903 34.0 30.5 36.0 20.0 38.0 80-81 30.819301787854023 34.0 31.0 35.5 19.5 37.0 82-83 30.399619270274133 34.0 30.0 35.0 18.0 37.0 84-85 29.928187191504485 34.0 30.0 35.0 14.0 36.0 86-87 29.50636320663107 34.0 29.0 35.0 7.5 36.0 88-89 29.0205495113541 34.0 29.0 35.0 4.5 35.5 90-91 28.416173134919035 33.0 28.5 35.0 2.0 35.0 92-93 27.864302648984783 33.0 27.0 35.0 2.0 35.0 94-95 27.31346551995808 33.0 26.0 34.5 2.0 35.0 96-97 26.582223696119783 32.0 25.0 34.0 2.0 35.0 98-99 25.5160217957691 32.0 22.0 34.0 2.0 35.0 100-101 22.46998574553769 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19650564714699925 1101 2 0.2188021410036356 1101 3 0.18172174230522842 1101 4 0.13670543181665096 1101 5 0.12105942418388338 1101 6 0.18482877774256679 1101 7 0.21914350912406633 1101 8 0.16696261428691628 1101 9 0.29968613921729315 1101 10-11 0.27011509532106714 1101 12-13 0.24040346965896475 1101 14-15 0.3169657616061343 1101 16-17 0.28402778772008475 1101 18-19 0.2926048189877477 1101 20-21 0.3067641460820596 1101 22-23 0.3196789633618806 1101 24-25 0.35009410049976 1101 26-27 0.16438605154096564 1101 28-29 0.1429320334201094 1101 30-31 0.41478481671153133 1101 32-33 0.3197535591654699 1101 34-35 0.3510145831692242 1101 36-37 0.4466020663257311 1101 38-39 0.42114120333236116 1101 40-41 0.41497347458636824 1101 42-43 0.48455685335793675 1101 44-45 0.4704540465110014 1101 46-47 0.6059959046882923 1101 48-49 0.6679961687593092 1101 50-51 0.45266596687109484 1101 52-53 0.631623576578427 1101 54-55 0.6468222993869901 1101 56-57 0.717803670360226 1101 58-59 0.7277373803472997 1101 60-61 0.6365677575967723 1101 62-63 0.8638247829894539 1101 64-65 0.7429532721107535 1101 66-67 0.704060052969858 1101 68-69 0.7773085633549073 1101 70-71 0.8305613730323813 1101 72-73 0.9076635759430047 1101 74-75 1.1182256101372126 1101 76-77 0.9961237912715042 1101 78-79 0.9366259337077238 1101 80-81 0.952318973970673 1101 82-83 0.8834554739585663 1101 84-85 1.134672053902662 1101 86-87 1.3197051122325014 1101 88-89 1.563281316559607 1101 90-91 1.5488333774813263 1101 92-93 1.5131794723691243 1101 94-95 1.6293084381827079 1101 96-97 1.8931638212776072 1101 98-99 2.1640220545976057 1101 100-101 2.6767921445724703 1102 1 0.27211752723953 1102 2 0.26284085306679117 1102 3 0.26339843701654786 1102 4 0.18249711100955324 1102 5 0.23450970667693838 1102 6 0.10751615256842939 1102 7 0.22131566404571146 1102 8 0.152881009573953 1102 9 0.25855492496999233 1102 10-11 0.2755109031608711 1102 12-13 0.34718767827616404 1102 14-15 0.27875686494510177 1102 16-17 0.3792448126723471 1102 18-19 0.42601521512727203 1102 20-21 0.4502830370551223 1102 22-23 0.3737174919421662 1102 24-25 0.24823922980578317 1102 26-27 0.2655112249675753 1102 28-29 0.10929821159891873 1102 30-31 0.3380981504118381 1102 32-33 0.41746312836180266 1102 34-35 0.4616039442677504 1102 36-37 0.4887840728661885 1102 38-39 0.4653196446594805 1102 40-41 0.428459465669107 1102 42-43 0.4123563432755617 1102 44-45 0.35412026011611175 1102 46-47 0.22667047240970106 1102 48-49 0.3721843770208366 1102 50-51 0.3994164146398731 1102 52-53 0.4250564423127159 1102 54-55 0.5567823142349866 1102 56-57 0.6954371082787958 1102 58-59 0.6059661496147655 1102 60-61 0.5556313457031337 1102 62-63 0.5184486219310074 1102 64-65 0.7492022753047678 1102 66-67 0.7125016764827663 1102 68-69 0.7729204999037123 1102 70-71 0.6443989455257579 1102 72-73 0.7341346498682526 1102 74-75 0.7262088087517746 1102 76-77 0.8565991812784652 1102 78-79 0.8204643389310604 1102 80-81 0.7285806657109752 1102 82-83 0.6491810146331893 1102 84-85 0.812246071598878 1102 86-87 1.05877976576744 1102 88-89 1.2578437310812838 1102 90-91 1.4545958687835352 1102 92-93 1.5852296676372397 1102 94-95 1.5751308860418156 1102 96-97 1.7439788380804053 1102 98-99 2.220336476492477 1102 100-101 3.127347744822572 1103 1 0.22485795366342387 1103 2 0.25764439611849355 1103 3 0.2708142583796658 1103 4 0.19587892005829133 1103 5 0.12776871183893235 1103 6 0.12025778758047778 1103 7 0.1405390139688052 1103 8 0.11454515992231507 1103 9 0.23623994990184372 1103 10-11 0.2086932101856931 1103 12-13 0.2372401479977313 1103 14-15 0.2800837153360476 1103 16-17 0.30874328635769643 1103 18-19 0.3773853575235009 1103 20-21 0.30532657927592055 1103 22-23 0.3382697430792305 1103 24-25 0.32546872086742695 1103 26-27 0.11843596108462151 1103 28-29 0.24313400601429436 1103 30-31 0.2327564740434127 1103 32-33 0.23973197879618624 1103 34-35 0.2639842019802856 1103 36-37 0.22828564355867798 1103 38-39 0.3686290517242483 1103 40-41 0.3092867725853381 1103 42-43 0.3947737461895784 1103 44-45 0.38275813405847714 1103 46-47 0.40082047141827815 1103 48-49 0.340252864312248 1103 50-51 0.3350763273760293 1103 52-53 0.2972208631771025 1103 54-55 0.47136204186831776 1103 56-57 0.5011770994221081 1103 58-59 0.3391037483200705 1103 60-61 0.33542257154199717 1103 62-63 0.41195170524711244 1103 64-65 0.6124821569386683 1103 66-67 0.6017843794146316 1103 68-69 0.5489200164684505 1103 70-71 0.4745944346593234 1103 72-73 0.2901517664979565 1103 74-75 0.13345981834777731 1103 76-77 0.3380940915171884 1103 78-79 0.44556265474458456 1103 80-81 0.4984555673951654 1103 82-83 0.595159956873065 1103 84-85 0.7399031007192463 1103 86-87 0.7649233592588125 1103 88-89 1.0052620262732752 1103 90-91 1.1257123587413922 1103 92-93 1.2475756316609719 1103 94-95 1.0655976748891902 1103 96-97 1.3747643637265519 1103 98-99 1.6060189086250567 1103 100-101 2.161821305540439 1104 1 0.18459267917544508 1104 2 0.2749333515955712 1104 3 0.2111775014547277 1104 4 0.18272446119120644 1104 5 0.07269332788170146 1104 6 0.14718988923816312 1104 7 0.09969778049058675 1104 8 0.13476576517928152 1104 9 0.1816970039179111 1104 10-11 0.26294539873725853 1104 12-13 0.2469124625194823 1104 14-15 0.29860977886932716 1104 16-17 0.36186902135359134 1104 18-19 0.29820396007290384 1104 20-21 0.26920969108419257 1104 22-23 0.34427564341795147 1104 24-25 0.1452028167136703 1104 26-27 0.2751713477457045 1104 28-29 0.2785730775165476 1104 30-31 0.27945174729057953 1104 32-33 0.28103064594188965 1104 34-35 0.2981958882067204 1104 36-37 0.3557767587893679 1104 38-39 0.3448077767992075 1104 40-41 0.2859683043029051 1104 42-43 0.3588415908251008 1104 44-45 0.24898888063837177 1104 46-47 0.33229338930801333 1104 48-49 0.4018040829933227 1104 50-51 0.34102018711612914 1104 52-53 0.3510503422768281 1104 54-55 0.4045984869234971 1104 56-57 0.4533604655042325 1104 58-59 0.32307088347041457 1104 60-61 0.4428606837299185 1104 62-63 0.6273821281047205 1104 64-65 0.5366856760295775 1104 66-67 0.6385837141912631 1104 68-69 0.73835265056411 1104 70-71 0.604921823824256 1104 72-73 0.6073222892501917 1104 74-75 0.5482521490186123 1104 76-77 0.6068502608528341 1104 78-79 0.5895940713629457 1104 80-81 0.6841004840477858 1104 82-83 0.4800690905455518 1104 84-85 0.640462669879696 1104 86-87 0.7329266150012756 1104 88-89 0.9998955144920458 1104 90-91 1.0627392991768652 1104 92-93 1.1933873356661984 1104 94-95 1.2973308854994272 1104 96-97 1.4494283722159267 1104 98-99 1.54958835427842 1104 100-101 2.2114334129021564 1105 1 0.0850878633154224 1105 2 0.09670856201351086 1105 3 0.05937724927107979 1105 4 -0.010634879316761214 1105 5 0.0677282415423619 1105 6 0.007169686290367849 1105 7 -0.07234692029739875 1105 8 -0.06520456615612602 1105 9 0.032486578914287634 1105 10-11 3.599765576467462E-5 1105 12-13 0.148981145478011 1105 14-15 0.18631123439988073 1105 16-17 0.24057186495381444 1105 18-19 0.2595229971128177 1105 20-21 0.21682639666155268 1105 22-23 0.15917761320536528 1105 24-25 0.08474055066344732 1105 26-27 -0.03157482976474313 1105 28-29 -0.0073834013780853525 1105 30-31 0.14677277283742285 1105 32-33 0.1721563598441378 1105 34-35 0.2670927966612311 1105 36-37 0.213649285959157 1105 38-39 0.29326465304202287 1105 40-41 0.1624583600022902 1105 42-43 0.13170897895835054 1105 44-45 0.21043242011005958 1105 46-47 0.29999427972114745 1105 48-49 0.41689095213685334 1105 50-51 0.4223083546169306 1105 52-53 0.3480190171117954 1105 54-55 0.22742843602761553 1105 56-57 0.462244729456458 1105 58-59 0.3451890495231069 1105 60-61 0.4251009857851784 1105 62-63 0.435651915161408 1105 64-65 0.33912504069631666 1105 66-67 0.4115809027320907 1105 68-69 0.46895013861718127 1105 70-71 0.5871568279036978 1105 72-73 0.46420586093356775 1105 74-75 0.4021856311662013 1105 76-77 0.4047492602418039 1105 78-79 0.5297538759735971 1105 80-81 0.44139226476810123 1105 82-83 0.4427369039296529 1105 84-85 0.5373980557665945 1105 86-87 0.8303749332471533 1105 88-89 1.081923632615716 1105 90-91 1.0633057410916216 1105 92-93 1.0816132908029417 1105 94-95 1.3580942531754197 1105 96-97 1.5191316325902484 1105 98-99 1.938100841607426 1105 100-101 2.5082675191512926 1106 1 -0.10229557812481005 1106 2 0.08754548033521559 1106 3 -0.03582369582844791 1106 4 0.08227575973992174 1106 5 -0.05895187347447006 1106 6 -0.061361174329739754 1106 7 -0.04633253576374585 1106 8 -0.11954221633556017 1106 9 -0.07825984166986188 1106 10-11 -0.26032889100527257 1106 12-13 -0.2908616351355491 1106 14-15 -0.19291459386245435 1106 16-17 -0.09578827591951011 1106 18-19 0.028069275040444097 1106 20-21 0.07938202899406122 1106 22-23 0.09467940012702769 1106 24-25 0.09958017351524262 1106 26-27 0.05708304717914103 1106 28-29 -0.040351363098579895 1106 30-31 0.03469359103207381 1106 32-33 0.115472678414001 1106 34-35 -0.3044119325514103 1106 36-37 0.026211976817990035 1106 38-39 0.1795183587683411 1106 40-41 0.16607121097822386 1106 42-43 0.1331392592932943 1106 44-45 0.24614020455009467 1106 46-47 0.2872796447955608 1106 48-49 0.17437949131910102 1106 50-51 0.37788886268222655 1106 52-53 0.054163278529891556 1106 54-55 0.10905230573464308 1106 56-57 0.11099202678387599 1106 58-59 0.26795762644990617 1106 60-61 0.27197368712035797 1106 62-63 0.35550513496392 1106 64-65 0.2658157435026425 1106 66-67 0.29144292711744413 1106 68-69 0.28762495602424565 1106 70-71 0.30035032811544937 1106 72-73 0.36323364180719153 1106 74-75 0.5438001018881771 1106 76-77 0.25470913809214935 1106 78-79 0.4212568962798642 1106 80-81 0.3952694389799625 1106 82-83 0.5790389048361142 1106 84-85 0.7824078634031792 1106 86-87 0.8037187800696977 1106 88-89 0.7290409676133756 1106 90-91 0.7597268824924832 1106 92-93 0.6831269731989771 1106 94-95 0.39832228072596365 1106 96-97 1.0038954320092195 1106 98-99 0.9817912620726936 1106 100-101 1.3833158143314357 1107 1 0.09288870760838819 1107 2 0.08936308786438474 1107 3 0.09011182355148861 1107 4 0.09869380568888886 1107 5 -0.03554048326004278 1107 6 0.12695128772462994 1107 7 0.027821974115830983 1107 8 0.10636833694719883 1107 9 0.14627001078428492 1107 10-11 0.1035165121120869 1107 12-13 0.14200462412979675 1107 14-15 0.1902112087881136 1107 16-17 0.19320351931310142 1107 18-19 0.17440795906232864 1107 20-21 0.10832449727103466 1107 22-23 0.08174856945579734 1107 24-25 0.03370372165158386 1107 26-27 0.24276508501791483 1107 28-29 0.18372385105190148 1107 30-31 0.16789173862331097 1107 32-33 0.14219775061708617 1107 34-35 0.19027024146607374 1107 36-37 0.1303539374509981 1107 38-39 0.09264343324107216 1107 40-41 0.2065526972932119 1107 42-43 0.20644856275809076 1107 44-45 0.05857767109646517 1107 46-47 0.060838089105082815 1107 48-49 0.24506640889504183 1107 50-51 0.22384331468585117 1107 52-53 0.29855158077854327 1107 54-55 0.3182403023377418 1107 56-57 0.2262226227359605 1107 58-59 0.22955995813206442 1107 60-61 0.4025066565539106 1107 62-63 0.4999020902348903 1107 64-65 0.5684041319978093 1107 66-67 0.5005829301814586 1107 68-69 0.4850821968544068 1107 70-71 0.5500694595222342 1107 72-73 0.5630066300031444 1107 74-75 0.6397032504534756 1107 76-77 0.6373628072573148 1107 78-79 0.7378631809274125 1107 80-81 0.6773577300695202 1107 82-83 0.7457944659396922 1107 84-85 0.771438384456971 1107 86-87 1.021865018983899 1107 88-89 1.0154630989237 1107 90-91 1.1572769896386497 1107 92-93 1.261883897981047 1107 94-95 1.2740634617358388 1107 96-97 1.2947060168778712 1107 98-99 1.6692244730343262 1107 100-101 2.2156937688893557 1108 1 0.09755470681675149 1108 2 0.11949370790210168 1108 3 0.12009342964243075 1108 4 0.07909986869384511 1108 5 0.1066407083858607 1108 6 0.1223421000834719 1108 7 0.09958941922514697 1108 8 0.19532593782265906 1108 9 0.19439639323789493 1108 10-11 0.08884236370351317 1108 12-13 0.1332782277061355 1108 14-15 0.2097595102619536 1108 16-17 0.11516072437026281 1108 18-19 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-0.22875087090348956 2316 46-47 -0.4116939426048063 2316 48-49 -0.7766917306590102 2316 50-51 -0.6614123104828664 2316 52-53 -0.503820342181875 2316 54-55 -0.49935290782585895 2316 56-57 -0.4254606519478443 2316 58-59 -0.67783228666692 2316 60-61 -0.31440226712705766 2316 62-63 -0.21450755983932623 2316 64-65 -0.298050443867524 2316 66-67 -0.29610526611712373 2316 68-69 -0.018104660339076872 2316 70-71 -0.22030544182222656 2316 72-73 -0.1974659686575464 2316 74-75 0.02303213040660168 2316 76-77 -0.07528735694534205 2316 78-79 -0.0701976204200001 2316 80-81 -0.02714515249244087 2316 82-83 -0.16216378173189838 2316 84-85 -0.019807529366339338 2316 86-87 0.1031163112083 2316 88-89 0.23396287533882187 2316 90-91 0.3024261595583404 2316 92-93 0.2147809878058382 2316 94-95 0.40735610788856746 2316 96-97 0.9235049848842856 2316 98-99 1.1939465718059488 2316 100-101 1.9025478713454937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 13.0 5 27.0 6 46.0 7 135.0 8 247.0 9 574.0 10 972.0 11 1604.0 12 2381.0 13 3556.0 14 4695.0 15 6458.0 16 8446.0 17 11713.0 18 10757.0 19 7383.0 20 6575.0 21 6998.0 22 7678.0 23 8433.0 24 9677.0 25 11960.0 26 14269.0 27 18066.0 28 22288.0 29 27840.0 30 35357.0 31 44505.0 32 56465.0 33 74351.0 34 101554.0 35 142711.0 36 212493.0 37 294744.0 38 196657.0 39 12845.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.4938832640091 19.756122418304823 15.186295578573203 22.56369873911287 2 15.714608180980036 20.635449936312828 39.62324164041739 24.02670024228975 3 19.50545443885422 24.437718260002857 29.93341810280069 26.12340919834223 4 13.105750521258935 17.089011605111086 37.26504871801449 32.54018915561549 5 13.722744035788203 37.43477330425249 34.33034902878183 14.512133631177482 6 31.96290175414167 36.676988021121524 17.22759869108125 14.132511533655556 7 28.69683433676442 32.50647680260318 20.574429180240163 18.222259680392234 8 26.551849232494313 35.43673986888826 19.431946851742598 18.579464046874826 9 26.036343382301748 16.894725848946674 20.246906341953192 36.822024426798386 10-11 24.9650600776117 27.10513490437784 27.19029523959589 20.739509778414565 12-13 25.746955761104616 24.072033458868834 28.128844349051963 22.052166430974594 14-15 22.52948914792028 25.61039513076362 26.339901134860405 25.520214586455698 16-17 22.076021356042755 28.990571534315144 26.930673477539145 22.002733632102956 18-19 21.943956877503233 27.43405020941967 29.302740594436727 21.319252318640366 20-21 22.464519580647643 26.513959479217437 29.685815527470073 21.33570541266485 22-23 22.129622606179 26.421085302678353 29.565478042311465 21.883814048831184 24-25 22.104062010231704 26.64189296397284 29.675932559145963 21.578112466649493 26-27 22.076674260632682 26.854716571128296 29.466701307952853 21.601907860286172 28-29 21.772004297740168 26.966230785129113 29.63586472965426 21.625900187476454 30-31 21.96067452580077 26.68110191064831 29.64998869536444 21.708234868186484 32-33 21.8982253377648 26.812497022686216 29.51318629372987 21.77609134581912 34-35 22.27005060517338 26.779920629394972 29.385519078626736 21.56450968680491 36-37 22.157517305063816 26.712569211094294 29.374232769140008 21.75568071470188 38-39 21.983715467740574 26.84144567364244 29.486802713111544 21.688036145505446 40-41 22.177891292319078 26.71011407234231 29.46771126102522 21.644283374313385 42-43 22.00128042896936 26.7982716041239 29.26055043787079 21.939897529035942 44-45 22.22201050213084 26.904070033748994 28.945133145472468 21.928786318647695 46-47 22.20775603982455 27.11381949966471 28.677449733782346 22.0009747267284 48-49 22.263857792500467 27.191284623869077 28.56139862292367 21.983458960706788 50-51 22.28826260457242 27.25295624356442 28.426365991564584 22.032415160298573 52-53 22.518422701605367 27.422910475380824 28.10551233615613 21.953154486857677 54-55 22.266532794424272 27.25288295584048 28.280523420924382 22.20006082881087 56-57 22.291670483735622 27.569266060088605 28.178433621476234 21.96062983469954 58-59 22.39145171987966 27.273843244887264 28.251977852449826 22.08272718278325 60-61 22.375731503094574 27.25775658948248 28.623913051444315 21.742598855978628 62-63 22.31537906243015 27.34262377380477 28.489100283257052 21.85289688050803 64-65 22.161951212362176 27.25020795391668 28.39782042309003 22.190020410631117 66-67 22.11904124999542 27.472672839935946 28.155347988435196 22.252937921633436 68-69 22.032818242780245 27.345298775728573 28.34985360777143 22.272029373719754 70-71 22.122742280054382 27.349879258474807 28.29162651110126 22.235751950369554 72-73 22.22363504978446 27.290722779780165 28.261567393340474 22.2240747770949 74-75 22.122998787088168 27.5737732551109 27.95999956027366 22.343228397527273 76-77 22.184120748853964 27.428443698538274 28.078395878298405 22.309039674309357 78-79 22.125197418806366 27.413126564234858 28.093713012601825 22.367963004356955 80-81 22.05681997237053 27.715768220244268 27.990450609570644 22.23696119781456 82-83 22.15310752309123 27.510133904589225 28.056679509175297 22.28007906314425 84-85 22.179910698744763 27.413799737263027 27.90940887845758 22.496880685534627 86-87 22.125417281978184 27.28245455245019 28.100015756860646 22.49211240871098 88-89 22.132855985958074 27.244308292139525 28.082573278562972 22.54026244333943 90-91 22.113225248468147 27.359494561552673 28.06095898443226 22.466321205546926 92-93 22.129927161901243 27.147280123447697 28.15390213523327 22.568890579417786 94-95 21.944872974052483 27.07340131991191 28.2199877609501 22.761737945085507 96-97 21.902989039820888 27.107736618577704 28.2070891215367 22.78218522006471 98-99 21.868184216697937 27.025533536585368 28.0773261608818 23.0289560858349 100-101 21.91476893544177 26.92760860395221 28.33683872592081 22.82078373468521 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 167.0 1 127.0 2 112.0 3 162.0 4 150.5 5 147.0 6 291.0 7 626.5 8 1098.0 9 1401.5 10 1363.5 11 1268.5 12 1332.5 13 1457.5 14 1611.0 15 1716.5 16 1796.0 17 1877.0 18 1986.5 19 2188.0 20 2411.5 21 2681.5 22 3106.0 23 3659.5 24 4423.0 25 5470.5 26 6927.0 27 9189.0 28 11300.5 29 13619.5 30 16437.5 31 18695.0 32 21204.0 33 24010.0 34 27282.5 35 30404.5 36 33597.5 37 36979.5 38 39428.0 39 40964.0 40 42420.5 41 45209.5 42 47901.0 43 49990.0 44 51946.5 45 53387.5 46 53456.5 47 53857.0 48 56266.0 49 56169.5 50 53541.5 51 50697.0 52 47707.0 53 45158.5 54 42699.0 55 39299.0 56 34971.0 57 30861.0 58 27595.5 59 24239.5 60 20224.5 61 16200.5 62 12512.5 63 9410.5 64 6971.0 65 5295.0 66 3958.0 67 3013.5 68 2167.5 69 1403.5 70 948.5 71 678.5 72 506.5 73 381.0 74 284.5 75 220.5 76 158.0 77 87.5 78 39.5 79 23.5 80 19.0 81 11.5 82 4.0 83 3.0 84 1.5 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04426578525964008 2 2.198631718194044E-4 3 0.0 4 0.0 5 9.527404112174189E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004653770470177393 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.229456534883125E-4 24-25 3.664386196990073E-5 26-27 0.001795549236525136 28-29 0.0034445230251706686 30-31 3.664386196990073E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.007218840808070444 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.198631718194044E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.397263436388088E-4 84-85 1.8321930984950365E-4 86-87 0.0 88-89 0.0 90-91 0.009160965492475182 92-93 0.0015023983407659299 94-95 0.0 96-97 0.0 98-99 3.664386196990073E-4 100-101 0.011616104244458532 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1364485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.52064408273873 #Duplication Level Percentage of deduplicated Percentage of total 1 82.98702012770633 54.373630092745216 2 10.459573598425667 13.706359979993177 3 2.848980629671492 5.6000113750596805 4 1.1511550627944285 3.0169768461358597 5 0.6374557889402939 2.0883256932819205 6 0.3891640789359165 1.5298968663448214 7 0.2615590824656341 1.1996263684186963 8 0.19129354299879495 1.0026940916920097 9 0.14510335143086847 0.855653853988304 >10 0.8453293570114109 10.113723412856213 >50 0.058794266723930344 2.6367012381335475 >100 0.023785749815512337 2.719268144197561 >500 3.3658417702826375E-4 0.16547260120901777 >1k 3.3658417702826375E-4 0.6193277055468569 >5k 1.1219472567608792E-4 0.37233173039701245 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5065 0.37120232175509443 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4281 0.31374474618629006 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2931 0.21480631886755808 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.328772393980146E-5 0.0 16-17 0.0 0.0 0.0 7.328772393980146E-5 0.0 18-19 0.0 0.0 0.0 7.328772393980146E-5 0.0 20-21 0.0 0.0 0.0 7.328772393980146E-5 0.0 22-23 0.0 0.0 0.0 7.328772393980146E-5 3.664386196990073E-5 24-25 0.0 0.0 0.0 7.328772393980146E-5 7.328772393980146E-5 26-27 0.0 0.0 0.0 2.565070337893051E-4 7.328772393980146E-5 28-29 0.0 0.0 0.0 8.794526872776176E-4 7.328772393980146E-5 30-31 0.0 0.0 0.0 0.0017589053745552352 7.328772393980146E-5 32-33 0.0 0.0 0.0 0.002858221233652257 7.328772393980146E-5 34-35 0.0 0.0 0.0 0.004617126608207492 7.328772393980146E-5 36-37 0.0 0.0 0.0 0.007035621498220941 7.328772393980146E-5 38-39 0.0 0.0 0.0 0.008867814596715977 7.328772393980146E-5 40-41 0.0 0.0 0.0 0.01249555693173615 7.328772393980146E-5 42-43 0.0 0.0 0.0 0.01758905374555235 7.328772393980146E-5 44-45 0.0 0.0 0.0 0.024258236624074284 7.328772393980146E-5 46-47 0.0 0.0 0.0 0.041737358783716934 7.328772393980146E-5 48-49 0.0 0.0 0.0 0.07878430323528657 7.328772393980146E-5 50-51 0.0 0.0 0.0 0.10758637874362856 7.328772393980146E-5 52-53 0.0 0.0 0.0 0.13298057508876976 7.328772393980146E-5 54-55 0.0 0.0 0.0 0.16526381748425228 7.328772393980146E-5 56-57 0.0 0.0 0.0 0.19325972802925645 7.328772393980146E-5 58-59 0.0 0.0 0.0 0.22961043910339798 7.328772393980146E-5 60-61 0.0 0.0 0.0 0.28670157605250335 7.328772393980146E-5 62-63 0.0 0.0 0.0 0.33928551797931084 7.328772393980146E-5 64-65 0.0 0.0 0.0 0.3986119305085802 7.328772393980146E-5 66-67 0.0 0.0 0.0 0.49850309823852956 7.328772393980146E-5 68-69 0.0 0.0 0.0 0.6088744104918706 7.328772393980146E-5 70-71 0.0 0.0 0.0 0.7096083870471277 7.328772393980146E-5 72-73 0.0 0.0 0.0 0.8173779851006058 7.328772393980146E-5 74-75 0.0 0.0 0.0 0.9201640179261772 7.328772393980146E-5 76-77 0.0 0.0 0.0 1.0171236766985345 7.328772393980146E-5 78-79 0.0 0.0 0.0 1.136546022858441 7.328772393980146E-5 80-81 0.0 0.0 0.0 1.322696841665537 7.328772393980146E-5 82-83 0.0 0.0 0.0 1.5231387666408938 7.328772393980146E-5 84-85 0.0 0.0 0.0 1.7195498667995617 7.328772393980146E-5 86-87 0.0 0.0 0.0 2.0008647951424896 7.328772393980146E-5 88-89 0.0 0.0 0.0 2.367633209599226 7.328772393980146E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3915 0.0 36.65819 1 TACCGCG 65 1.3272541E-4 36.543427 8 GTATCAA 6205 0.0 33.008923 1 ATCAACG 7460 0.0 27.441729 3 TCAACGC 7500 0.0 27.295372 4 TACCGTA 70 0.008394052 27.141571 2 TATCAAC 7625 0.0 26.972492 2 CAACGCA 7600 0.0 26.936222 5 AACGCAG 7805 0.0 26.411304 6 ATAGGCG 90 8.9508883E-4 26.387636 5 GTACATG 5010 0.0 23.429071 1 ACGCAGA 8810 0.0 23.025259 7 CGCAGAG 8875 0.0 22.749569 8 GAGTACT 4855 0.0 22.261005 12-13 TACATGG 5190 0.0 21.78124 2 GCAGAGT 9530 0.0 21.086283 9 GTACTTT 5125 0.0 20.852669 14-15 ACATGGG 5170 0.0 20.579294 3 AGTACTT 5140 0.0 20.425924 12-13 TACCTGG 1025 0.0 20.389277 2 >>END_MODULE