FastQCFastQC Report
Thu 26 May 2016
SRR937254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335187
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43450.32542258125640827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34020.25479577018050653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21500.1610261334180156No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18410.13788330773142637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35400.035.063561
GTATCAA55100.032.36671
ATCAACG65350.026.624093
TCAACGC65550.026.5458474
CAACGCA66600.026.1987175
TATCAAC66900.026.0792772
TACCTGG10300.025.8467752
GTACCTG11450.025.7516421
AACGCAG68300.025.7322566
GTACATG53950.023.9770741
TACATGG54050.023.483952
ACATGGG54800.022.2941973
ACGCAGA78500.022.1474937
GAGTACT38750.021.81910912-13
CGCAGAG79100.021.7993378
TACACCG1656.279633E-520.1698325
CGTACAC1656.28508E-520.167563
GCAGAGT84050.019.9503359
AGAGTAC68400.019.7231110-11
AGTACTT42200.019.69764512-13
Produced by FastQC (version 0.11.5)