FastQCFastQC Report
Thu 26 May 2016
SRR937254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335187
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51660.38691209545928773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44090.3302159173209446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29360.21989429196060178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60650.032.3645631
GGTATCA39700.031.4858741
ATCAACG73350.026.8096053
TCAACGC73900.026.674354
CAACGCA74800.026.3534015
TATCAAC76000.025.8747962
AACGCAG76400.025.8617326
GTACATG52200.023.5819531
ACGCAGA87350.022.5679247
TACATGG53550.022.4414652
CGCAGAG88400.022.0849298
TACCTGG10650.021.8542882
ACATGGG52650.021.4718153
GAGTACT47200.021.33537712-13
GTACCTG11400.020.845661
GTAGGAC3650.020.8217533
AGTACTT50150.020.60130712-13
GCAGAGT95000.020.0005919
AGAGTAC78400.019.9033310-11
GTACTTT52700.019.19670314-15
Produced by FastQC (version 0.11.5)