FastQCFastQC Report
Thu 26 May 2016
SRR937253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395083
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45880.3288693217536161No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34130.24464494227225192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22420.16070728408273915No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19820.14207040011239475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35450.034.864051
GTATCAA57050.033.829161
ATCAACG67200.028.2869823
TCAACGC68150.027.7551984
TATCAAC68750.027.5801122
CAACGCA68750.027.511985
AACGCAG71300.026.5813066
GTACATG52700.024.8954091
TACATGG54400.023.1495652
ACGCAGA81650.022.9217957
CGCAGAG81950.022.6648048
ACATGGG54050.022.2443983
GTCCTAC2901.05501385E-1021.309151
GAGTACT41200.021.2719712-13
GTACCTG12800.021.1682851
TACCTGG12450.020.993712
GCAGAGT86050.020.9224479
GTATAGC6100.020.2611581
AGAGTAC70900.020.09942210-11
AGTACTT44450.019.71665212-13
Produced by FastQC (version 0.11.5)